(Circular) (Six-base) MAPSORT of: pFN21AB4593.seq Check: 340 from: 1 to: 7045 pFN21AB4593 7045 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4855 278 Size: 53 83 186 936 3319 2468 Fragments arranged by size: 3319 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2120 4388 1937 Size: 183 2268 4594 Fragments arranged by size: 4594 2268 183 Acc65I G'GTAC_C Cuts at: 4366 4366 Size: 7045 AclI AA'CG_TT Cuts at: 1563 2471 5172 5545 1563 Size: 908 2701 373 3063 Fragments arranged by size: 3063 2701 908 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3102 3678 3760 4164 4317 5108 6492 6760 Size: 1470 576 82 404 153 791 1384 268 Cuts at: 6760 1632 Size: 1917 Fragments arranged by size: 1917 1470 1384 791 576 404 268 153 82 AfeI AGC'GCT Cuts at: 2438 2438 Size: 7045 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6848 AflIII A'CryG_T Cuts at: 1203 3601 5944 6921 1203 Size: 2398 2343 977 1327 Fragments arranged by size: 2398 2343 1327 977 AgeI A'CCGG_T Cuts at: 5876 5876 Size: 7045 AhdI GACnn_n'nnGTC Cuts at: 5774 5774 Size: 7045 AleI CACnn'nnGTG Cuts at: 1166 3140 1166 Size: 1974 5071 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7045 AlwNI CAG_nnn'CTG Cuts at: 2159 3899 3992 4788 6360 2159 Size: 1740 93 796 1572 2844 Fragments arranged by size: 2844 1740 1572 796 93 ApaLI G'TGCA_C Cuts at: 5102 6258 5102 Size: 1156 5889 ApoI r'AATT_y Cuts at: 1493 2030 2278 2408 2487 2546 2749 2780 2910 Size: 537 248 130 79 59 203 31 130 Cuts at: 2910 3301 3481 3550 4354 4580 4732 6841 1493 Size: 391 180 69 804 226 152 2109 1697 Fragments arranged by size: 2109 1697 804 537 391 248 226 203 180 152 130 130 79 69 59 31 AseI AT'TA_AT Cuts at: 160 1032 5599 160 Size: 872 4567 1606 Fragments arranged by size: 4567 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7045 AvaI C'yCGr_G Cuts at: 1870 1943 2091 4370 1870 Size: 73 148 2279 4545 Fragments arranged by size: 4545 2279 148 73 BamHI G'GATC_C Cuts at: 4375 4742 4375 Size: 367 6678 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2720 4366 5821 618 Size: 325 217 627 933 1646 1455 1842 Fragments arranged by size: 1842 1646 1455 933 627 325 217 BanII G_rGCy'C Cuts at: 1391 2923 4364 1391 Size: 1532 1441 4072 Fragments arranged by size: 4072 1532 1441 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2466 928 Size: 971 567 5507 Fragments arranged by size: 5507 971 567 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5236 5270 1903 Size: 34 3299 34 3678 Fragments arranged by size: 3678 3299 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4937 6147 4937 Size: 1210 5835 BclI T'GATC_A Cuts at: 1548 2215 2385 4177 1548 Size: 667 170 1792 4416 Fragments arranged by size: 4416 1792 667 170 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5656 136 Size: 107 122 71 631 605 3984 1525 Fragments arranged by size: 3984 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3454 7040 3454 Size: 3586 3459 BlpI GC'TnA_GC Cuts at: 4462 4462 Size: 7045 Bme1580I G_kGCm'C Cuts at: 1790 5106 6262 1790 Size: 3316 1156 2573 Fragments arranged by size: 3316 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7045 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4776 5734 442 Size: 416 53 83 399 24 3359 958 1753 Fragments arranged by size: 3359 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7045 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2181 5705 1239 Size: 942 3524 2579 Fragments arranged by size: 3524 2579 942 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2307 5170 6035 6333 2307 Size: 2863 865 298 3019 Fragments arranged by size: 3019 2863 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5708 882 Size: 617 4209 2219 Fragments arranged by size: 4209 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2745 4836 493 Size: 711 1541 2091 2702 Fragments arranged by size: 2702 2091 1541 711 BsaBI GATnn'nnATC Cuts at: 7039 7039 Size: 7045 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2292 4852 5234 5869 Size: 53 83 186 936 759 2560 382 635 Cuts at: 5869 275 Size: 1451 Fragments arranged by size: 2560 1451 936 759 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3073 5478 5876 6150 6297 6708 3073 Size: 2405 398 274 147 411 3410 Fragments arranged by size: 3410 2405 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3094 3124 1178 Size: 30 165 30 1691 30 5099 Fragments arranged by size: 5099 1691 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2991 2994 4017 1186 Size: 1805 3 1023 4214 Fragments arranged by size: 4214 1805 1023 3 BseYI C'CCAG_C Cuts at: 2762 6248 6744 2762 Size: 3486 496 3063 Fragments arranged by size: 3486 3063 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2721 1163 Size: 573 985 5487 Fragments arranged by size: 5487 985 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4495 5256 6284 1540 Size: 275 186 2494 761 1028 2301 Fragments arranged by size: 2494 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2923 5106 5191 6262 2923 Size: 2183 85 1071 3706 Fragments arranged by size: 3706 2183 1071 85 BsmI GAATG_Cn' Cuts at: 2824 4556 4649 2824 Size: 1732 93 5220 Fragments arranged by size: 5220 1732 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2923 4364 5106 5191 6262 1391 Size: 399 1133 1441 742 85 1071 2174 Fragments arranged by size: 2174 1441 1133 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6708 6708 Size: 7045 BspHI T'CATG_A Cuts at: 3754 4934 6664 3754 Size: 1180 1730 4135 Fragments arranged by size: 4135 1730 1180 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4400 844 Size: 3556 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4932 1394 Size: 429 3109 3507 Fragments arranged by size: 3507 3109 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2692 3951 5540 5714 65 Size: 1183 1444 1259 1589 174 1396 Fragments arranged by size: 1589 1444 1396 1259 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5689 5876 1700 Size: 110 3879 187 2869 Fragments arranged by size: 3879 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7045 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7045 BssSI C'ACGA_G Cuts at: 5105 6117 5105 Size: 1012 6033 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7045 BstBI TT'CG_AA Cuts at: 5849 5849 Size: 7045 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7045 BstYI r'GATC_y Cuts at: 1972 2019 3454 4324 4375 4742 5127 5144 6596 Size: 47 1435 870 51 367 385 17 1452 Cuts at: 6596 6682 6694 7040 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1435 870 385 367 346 86 51 47 17 12 Bsu36I CC'TnA_GG Cuts at: 2024 2024 Size: 7045 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5554 Fragments arranged by size: 5554 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6306 Fragments arranged by size: 6306 683 56 BtsI GCAGTG_nn' Cuts at: 2704 3505 4561 5355 5375 2704 Size: 801 1056 794 20 4374 Fragments arranged by size: 4374 1056 801 794 20 ClaI AT'CG_AT Cuts at: 1554 4297 1554 Size: 2743 4302 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3081 3116 4264 4299 529 Size: 35 2517 35 1148 35 3275 Fragments arranged by size: 3275 2517 1148 35 35 35 DraI TTT'AAA Cuts at: 2485 4349 2485 Size: 1864 5181 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6052 809 Size: 507 4736 1802 Fragments arranged by size: 4736 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2594 4492 5381 Size: 54 1425 50 275 18 764 1898 889 Cuts at: 5381 7010 8 Size: 1629 43 Fragments arranged by size: 1898 1629 1425 889 764 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4492 1537 Size: 275 2680 4090 Fragments arranged by size: 4090 2680 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2051 3779 4975 1353 Size: 698 1728 1196 3423 Fragments arranged by size: 3423 1728 1196 698 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5632 6006 6152 387 Size: 1064 138 4043 374 146 1280 Fragments arranged by size: 4043 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2921 2921 Size: 7045 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2181 3102 3678 3760 4164 4317 5108 Size: 393 549 921 576 82 404 153 791 Cuts at: 5108 5705 6492 6760 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 921 791 787 597 576 549 404 393 268 153 82 EcoO109I rG'GnC_Cy Cuts at: 2327 3021 3325 2327 Size: 694 304 6047 Fragments arranged by size: 6047 694 304 EcoRI G'AATT_C Cuts at: 3481 3550 4354 4732 3481 Size: 69 804 378 5794 Fragments arranged by size: 5794 804 378 69 EcoRV GAT'ATC Cuts at: 3676 3676 Size: 7045 FspI TGC'GCA Cuts at: 5551 5551 Size: 7045 HaeII r_GCGC'y Cuts at: 1678 2440 6192 1678 Size: 762 3752 2531 Fragments arranged by size: 3752 2531 762 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2644 2676 3094 3126 3413 Size: 32 135 32 1072 32 418 32 287 Cuts at: 3413 3445 3809 3841 5686 5718 5760 5792 1373 Size: 32 364 32 1845 32 42 32 2626 Fragments arranged by size: 2626 1845 1072 418 364 287 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4389 4635 669 Size: 988 281 2451 246 3079 Fragments arranged by size: 3079 2451 988 281 246 HindIII A'AGCT_T Cuts at: 748 2644 748 Size: 1896 5149 HpaI GTT'AAC Cuts at: 4635 4635 Size: 7045 KpnI G_GTAC'C Cuts at: 4370 4370 Size: 7045 MfeI C'AATT_G Cuts at: 3270 4644 3270 Size: 1374 5671 MluI A'CGCG_T Cuts at: 6921 6921 Size: 7045 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3152 4289 6159 6343 1305 Size: 1847 1137 1870 184 2007 Fragments arranged by size: 2007 1870 1847 1137 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2596 10 Size: 54 1425 343 764 4459 Fragments arranged by size: 4459 1425 764 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2393 2673 2697 3140 3813 3987 5003 Size: 648 1227 280 24 443 673 174 1016 Cuts at: 5003 5362 5521 518 Size: 359 159 2042 Fragments arranged by size: 2042 1227 1016 673 648 443 359 280 174 159 24 MspA1I CmG'CkG Cuts at: 1606 4187 4461 5138 6286 6531 1606 Size: 2581 274 677 1148 245 2120 Fragments arranged by size: 2581 2120 1148 677 274 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5554 Fragments arranged by size: 5554 939 552 NdeI CA'TA_TG Cuts at: 387 2311 3186 387 Size: 1924 875 4246 Fragments arranged by size: 4246 1924 875 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7045 NotI GC'GGCC_GC Cuts at: 4492 4492 Size: 7045 NspI r_CATG'y Cuts at: 1132 1229 3386 3605 4403 5948 1132 Size: 97 2157 219 798 1545 2229 Fragments arranged by size: 2229 2157 1545 798 219 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7045 PciI A'CATG_T Cuts at: 3601 5944 3601 Size: 2343 4702 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7045 PmeI GTTT'AAAC Cuts at: 4349 4349 Size: 7045 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5114 5146 6627 6659 5114 Size: 32 1481 32 5500 Fragments arranged by size: 5500 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2327 3021 3325 2327 Size: 694 304 6047 Fragments arranged by size: 6047 694 304 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7045 PsiI TTA'TAA Cuts at: 2285 4615 2285 Size: 2330 4715 PstI C_TGCA'G Cuts at: 830 2833 3069 3500 4397 830 Size: 2003 236 431 897 3478 Fragments arranged by size: 3478 2003 897 431 236 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7045 SacI G_AGCT'C Cuts at: 2923 2923 Size: 7045 SalI G'TCGA_C Cuts at: 1936 4387 1936 Size: 2451 4594 SbfI CC_TGCA'GG Cuts at: 4397 4397 Size: 7045 ScaI AGT'ACT Cuts at: 3913 5293 7005 3913 Size: 1380 1712 3953 Fragments arranged by size: 3953 1712 1380 SfcI C'TryA_G Cuts at: 826 1045 2829 3065 3229 3496 4393 5528 6209 Size: 219 1784 236 164 267 897 1135 681 Cuts at: 6209 6400 826 Size: 191 1471 Fragments arranged by size: 1784 1471 1135 897 681 267 236 219 191 164 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7045 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7045 SmaI CCC'GGG Cuts at: 1872 2093 4372 1872 Size: 221 2279 4545 Fragments arranged by size: 4545 2279 221 SmlI C'TyrA_G Cuts at: 820 1017 1943 2322 5149 6050 6312 820 Size: 197 926 379 2827 901 262 1553 Fragments arranged by size: 2827 1553 926 901 379 262 197 SnaBI TAC'GTA Cuts at: 493 4836 493 Size: 4343 2702 SpeI A'CTAG_T Cuts at: 152 3908 152 Size: 3756 3289 SphI G_CATG'C Cuts at: 1132 4403 1132 Size: 3271 3774 SspI AAT'ATT Cuts at: 5 52 3874 4969 5 Size: 47 3822 1095 2081 Fragments arranged by size: 3822 2081 1095 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7045 StyI C'CwwG_G Cuts at: 513 1065 2004 3017 3566 4484 513 Size: 552 939 1013 549 918 3074 Fragments arranged by size: 3074 1013 939 918 552 549 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3109 5072 5089 5242 5427 5872 325 Size: 1255 1529 1963 17 153 185 445 1498 Fragments arranged by size: 1963 1529 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2833 3911 5291 Size: 274 80 33 51 1443 856 1078 1380 Cuts at: 5291 7003 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1380 1078 856 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2416 2448 7005 7037 2416 Size: 32 4557 32 2424 Fragments arranged by size: 4557 2424 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7045 XbaI T'CTAG_A Cuts at: 4381 4381 Size: 7045 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 4335 2011 Size: 2324 4721 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7045 XmaI C'CCGG_G Cuts at: 1870 2091 4370 1870 Size: 221 2279 4545 Fragments arranged by size: 4545 2279 221 XmnI GAAnn'nnTTC Cuts at: 2676 2859 5174 2676 Size: 183 2315 4547 Fragments arranged by size: 4547 2315 183 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4853 276 Size: 53 83 186 936 3319 2468 Fragments arranged by size: 3319 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI