(Circular) (Six-base) MAPSORT of: pFN21AB4575.seq Check: 8710 from: 1 to: 7360 pFN21AB4575 7360 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5170 278 Size: 53 83 186 936 3634 2468 Fragments arranged by size: 3634 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4703 1937 Size: 2766 4594 Acc65I G'GTAC_C Cuts at: 3313 3928 4681 3313 Size: 615 753 5992 Fragments arranged by size: 5992 753 615 AclI AA'CG_TT Cuts at: 1563 2431 3073 3230 5487 5860 1563 Size: 868 642 157 2257 373 3063 Fragments arranged by size: 3063 2257 868 642 373 157 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2144 2463 2737 3180 3347 3668 5423 6807 Size: 512 319 274 443 167 321 1755 1384 Cuts at: 6807 7075 1632 Size: 268 1917 Fragments arranged by size: 1917 1755 1384 512 443 321 319 274 268 167 AfeI AGC'GCT Cuts at: 4574 4574 Size: 7360 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7163 AflIII A'CryG_T Cuts at: 1203 2545 6259 7236 1203 Size: 1342 3714 977 1327 Fragments arranged by size: 3714 1342 1327 977 AgeI A'CCGG_T Cuts at: 6191 6191 Size: 7360 AhdI GACnn_n'nnGTC Cuts at: 6089 6089 Size: 7360 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7360 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3414 1184 Size: 2230 5130 AlwNI CAG_nnn'CTG Cuts at: 3752 5103 6675 3752 Size: 1351 1572 4437 Fragments arranged by size: 4437 1572 1351 ApaLI G'TGCA_C Cuts at: 3899 5417 6573 3899 Size: 1518 1156 4686 Fragments arranged by size: 4686 1518 1156 ApoI r'AATT_y Cuts at: 1493 2910 3112 3255 3463 4669 4895 5047 7156 Size: 1417 202 143 208 1206 226 152 2109 Cuts at: 7156 1493 Size: 1697 Fragments arranged by size: 2109 1697 1417 1206 226 208 202 152 143 AseI AT'TA_AT Cuts at: 160 1032 5914 160 Size: 872 4882 1606 Fragments arranged by size: 4882 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7360 AvaI C'yCGr_G Cuts at: 1870 1943 2176 4685 1870 Size: 73 233 2509 4545 Fragments arranged by size: 4545 2509 233 73 BamHI G'GATC_C Cuts at: 4690 5057 4690 Size: 367 6993 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3313 3928 4681 6136 618 Size: 325 217 627 1526 615 753 1455 1842 Fragments arranged by size: 1842 1526 1455 753 627 615 325 217 BanII G_rGCy'C Cuts at: 1391 2333 3599 4679 1391 Size: 942 1266 1080 4072 Fragments arranged by size: 4072 1266 1080 942 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4548 928 Size: 971 2649 3740 Fragments arranged by size: 3740 2649 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5551 5585 1903 Size: 34 3614 34 3678 Fragments arranged by size: 3678 3614 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5252 6462 5252 Size: 1210 6150 BclI T'GATC_A Cuts at: 1548 1548 Size: 7360 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5971 136 Size: 107 122 71 631 605 4299 1525 Fragments arranged by size: 4299 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7355 7355 Size: 7360 BlpI GC'TnA_GC Cuts at: 4569 4777 4569 Size: 208 7152 Bme1580I G_kGCm'C Cuts at: 1790 2494 2990 3903 5421 6577 1790 Size: 704 496 913 1518 1156 2573 Fragments arranged by size: 2573 1518 1156 913 704 496 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7360 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5091 6049 442 Size: 416 53 83 399 24 3674 958 1753 Fragments arranged by size: 3674 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7360 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3594 3594 Size: 7360 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2922 3407 3879 4548 6020 1239 Size: 1683 485 472 669 1472 2579 Fragments arranged by size: 2579 1683 1472 669 485 472 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2317 4597 5485 6350 6648 2317 Size: 2280 888 865 298 3029 Fragments arranged by size: 3029 2280 888 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6023 882 Size: 617 4524 2219 Fragments arranged by size: 4524 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5151 493 Size: 711 3947 2702 Fragments arranged by size: 3947 2702 711 BsaBI GATnn'nnATC Cuts at: 2614 4269 7354 2614 Size: 1655 3085 2620 Fragments arranged by size: 3085 2620 1655 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4171 5167 5549 6184 Size: 53 83 186 936 2638 996 382 635 Cuts at: 6184 275 Size: 1451 Fragments arranged by size: 2638 1451 996 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5793 6191 6465 6612 7023 5793 Size: 398 274 147 411 6130 Fragments arranged by size: 6130 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3791 3821 1178 Size: 30 165 30 2388 30 4717 Fragments arranged by size: 4717 2388 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2164 2341 2732 4483 4529 1186 Size: 978 177 391 1751 46 4017 Fragments arranged by size: 4017 1751 978 391 177 46 BseYI C'CCAG_C Cuts at: 2797 6563 7059 2797 Size: 3766 496 3098 Fragments arranged by size: 3766 3098 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3589 3771 1163 Size: 573 1853 182 4752 Fragments arranged by size: 4752 1853 573 182 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4810 5571 6599 1540 Size: 275 186 2809 761 1028 2301 Fragments arranged by size: 2809 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2333 2788 3580 3599 3903 3969 4012 5421 5506 Size: 455 792 19 304 66 43 1409 85 Cuts at: 5506 6577 2333 Size: 1071 3116 Fragments arranged by size: 3116 1409 1071 792 455 304 85 66 43 19 BsmI GAATG_Cn' Cuts at: 3403 4871 4964 3403 Size: 1468 93 5799 Fragments arranged by size: 5799 1468 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2333 2494 2788 2990 3580 3599 3903 Size: 399 543 161 294 202 590 19 304 Cuts at: 3903 3969 4012 4679 5421 5506 6577 1391 Size: 66 43 667 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 667 590 543 399 304 294 202 161 85 66 43 19 BspEI T'CCGG_A Cuts at: 7023 7023 Size: 7360 BspHI T'CATG_A Cuts at: 3778 4174 4390 5249 6979 3778 Size: 396 216 859 1730 4159 Fragments arranged by size: 4159 1730 859 396 216 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4715 844 Size: 3871 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5247 1394 Size: 429 3424 3507 Fragments arranged by size: 3507 3424 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3373 5855 6029 65 Size: 1183 2125 2482 174 1396 Fragments arranged by size: 2482 2125 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6004 6191 1700 Size: 110 4194 187 2869 Fragments arranged by size: 4194 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7360 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7360 BssSI C'ACGA_G Cuts at: 5420 6432 5420 Size: 1012 6348 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7360 BstBI TT'CG_AA Cuts at: 6164 6164 Size: 7360 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7360 BstXI CCAn_nnnn'nTGG Cuts at: 2032 2032 Size: 7360 BstYI r'GATC_y Cuts at: 1972 4690 5057 5442 5459 6911 6997 7009 7355 Size: 2718 367 385 17 1452 86 12 346 Cuts at: 7355 1972 Size: 1977 Fragments arranged by size: 2718 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6808 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4521 487 Size: 683 56 3295 3326 Fragments arranged by size: 3326 3295 683 56 BtsI GCAGTG_nn' Cuts at: 3709 4417 4876 5670 5690 3709 Size: 708 459 794 20 5379 Fragments arranged by size: 5379 794 708 459 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7360 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7325 DraI TTT'AAA Cuts at: 3001 3078 4664 3001 Size: 77 1586 5697 Fragments arranged by size: 5697 1586 77 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2756 6367 809 Size: 507 1440 3611 1802 Fragments arranged by size: 3611 1802 1440 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4807 5696 7325 Size: 54 1425 50 275 18 2977 889 1629 Cuts at: 7325 8 Size: 43 Fragments arranged by size: 2977 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4807 1537 Size: 275 2995 4090 Fragments arranged by size: 4090 2995 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4088 4167 4228 4501 4506 4563 4624 5290 Size: 2735 79 61 273 5 57 61 666 Cuts at: 5290 1353 Size: 3423 Fragments arranged by size: 3423 2735 666 273 79 61 61 57 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2212 5947 6321 6467 387 Size: 1064 138 623 3735 374 146 1280 Fragments arranged by size: 3735 1280 1064 623 374 146 138 EcoICRI GAG'CTC Cuts at: 2331 3597 2331 Size: 1266 6094 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2463 2737 2922 3180 3347 3407 Size: 393 512 319 274 185 258 167 60 Cuts at: 3407 3668 3879 4548 5423 6020 6807 7075 1239 Size: 261 211 669 875 597 787 268 1524 Fragments arranged by size: 1524 875 787 669 597 512 393 319 274 268 261 258 211 185 167 60 EcoNI CCTnn'n_nnAGG Cuts at: 2394 3570 2394 Size: 1176 6184 EcoO109I rG'GnC_Cy Cuts at: 2801 3944 2801 Size: 1143 6217 EcoRI G'AATT_C Cuts at: 3255 4669 5047 3255 Size: 1414 378 5568 Fragments arranged by size: 5568 1414 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3875 3875 Size: 7360 FspI TGC'GCA Cuts at: 5866 5866 Size: 7360 HaeII r_GCGC'y Cuts at: 1678 2316 4576 6507 1678 Size: 638 2260 1931 2531 Fragments arranged by size: 2531 2260 1931 638 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2138 2170 3110 3142 4475 Size: 32 135 32 566 32 940 32 1333 Cuts at: 4475 4478 4507 4510 6001 6033 6075 6107 1373 Size: 3 29 3 1491 32 42 32 2626 Fragments arranged by size: 2626 1491 1333 940 566 135 42 32 32 32 32 32 32 29 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 2853 3234 4704 4950 669 Size: 988 281 915 381 1470 246 3079 Fragments arranged by size: 3079 1470 988 915 381 281 246 HindIII A'AGCT_T Cuts at: 748 3668 4286 748 Size: 2920 618 3822 Fragments arranged by size: 3822 2920 618 HpaI GTT'AAC Cuts at: 3234 4950 3234 Size: 1716 5644 KpnI G_GTAC'C Cuts at: 3317 3932 4685 3317 Size: 615 753 5992 Fragments arranged by size: 5992 753 615 MfeI C'AATT_G Cuts at: 4959 4959 Size: 7360 MluI A'CGCG_T Cuts at: 7236 7236 Size: 7360 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3294 4570 6474 6658 1305 Size: 1989 1276 1904 184 2007 Fragments arranged by size: 2007 1989 1904 1276 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5538 Fragments arranged by size: 5538 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2036 3816 3827 4395 5318 5677 5836 Size: 648 870 1780 11 568 923 359 159 Cuts at: 5836 518 Size: 2042 Fragments arranged by size: 2042 1780 923 870 648 568 359 159 11 MspA1I CmG'CkG Cuts at: 1606 3522 3739 4776 5453 6601 6846 1606 Size: 1916 217 1037 677 1148 245 2120 Fragments arranged by size: 2120 1916 1148 1037 677 245 217 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6808 NdeI CA'TA_TG Cuts at: 387 387 Size: 7360 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7360 NotI GC'GGCC_GC Cuts at: 4807 4807 Size: 7360 NspI r_CATG'y Cuts at: 1132 1229 2549 2728 2874 3832 4718 6263 1132 Size: 97 1320 179 146 958 886 1545 2229 Fragments arranged by size: 2229 1545 1320 958 886 179 146 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7360 PciI A'CATG_T Cuts at: 2545 6259 2545 Size: 3714 3646 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2213 1108 Size: 902 203 6255 Fragments arranged by size: 6255 902 203 PfoI T'CCnGG_A Cuts at: 3910 3910 Size: 7360 PmeI GTTT'AAAC Cuts at: 4664 4664 Size: 7360 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5429 5461 6942 6974 5429 Size: 32 1481 32 5815 Fragments arranged by size: 5815 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2801 3944 2801 Size: 1143 6217 PshAI GACnn'nnGTC Cuts at: 1613 4458 1613 Size: 2845 4515 PsiI TTA'TAA Cuts at: 2816 4930 2816 Size: 2114 5246 PstI C_TGCA'G Cuts at: 830 3744 3762 4412 4712 830 Size: 2914 18 650 300 3478 Fragments arranged by size: 3478 2914 650 300 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7360 PvuII CAG'CTG Cuts at: 3739 3739 Size: 7360 SacI G_AGCT'C Cuts at: 2333 3599 2333 Size: 1266 6094 SalI G'TCGA_C Cuts at: 1936 4702 1936 Size: 2766 4594 SanDI GG'GwC_CC Cuts at: 3944 3944 Size: 7360 SbfI CC_TGCA'GG Cuts at: 4712 4712 Size: 7360 ScaI AGT'ACT Cuts at: 3432 5608 7320 3432 Size: 2176 1712 3472 Fragments arranged by size: 3472 2176 1712 SfcI C'TryA_G Cuts at: 826 1045 3740 3758 4408 4456 4602 4708 5843 Size: 219 2695 18 650 48 146 106 1135 Cuts at: 5843 6524 6715 826 Size: 681 191 1471 Fragments arranged by size: 2695 1471 1135 681 650 219 191 146 106 48 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7360 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7360 SmaI CCC'GGG Cuts at: 1872 4687 1872 Size: 2815 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2332 4576 5464 6365 6627 820 Size: 197 926 389 2244 888 901 262 1553 Fragments arranged by size: 2244 1553 926 901 888 389 262 197 SnaBI TAC'GTA Cuts at: 493 5151 493 Size: 4658 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7360 SphI G_CATG'C Cuts at: 1132 4718 1132 Size: 3586 3774 SspI AAT'ATT Cuts at: 5 52 2595 5284 5 Size: 47 2543 2689 2081 Fragments arranged by size: 2689 2543 2081 47 StuI AGG'CCT Cuts at: 1517 2389 3656 1517 Size: 872 1267 5221 Fragments arranged by size: 5221 1267 872 StyI C'CwwG_G Cuts at: 513 1065 2226 4799 513 Size: 552 1161 2573 3074 Fragments arranged by size: 3074 2573 1161 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5387 5404 5557 5742 6187 325 Size: 1255 3807 17 153 185 445 1498 Fragments arranged by size: 3807 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2763 3430 4478 Size: 274 80 33 51 1443 786 667 1048 Cuts at: 4478 5606 7318 96 Size: 1128 1712 138 Fragments arranged by size: 1712 1443 1128 1048 786 667 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2767 2799 3791 3823 3846 3878 4110 4142 7320 Size: 32 992 32 23 32 232 32 3178 Cuts at: 7320 7352 2767 Size: 32 2775 Fragments arranged by size: 3178 2775 992 232 32 32 32 32 32 23 Tth111I GACn'n_nGTC Cuts at: 1316 2193 1316 Size: 877 6483 XbaI T'CTAG_A Cuts at: 4696 4696 Size: 7360 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2032 4208 2011 Size: 21 2176 5163 Fragments arranged by size: 5163 2176 21 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7360 XmaI C'CCGG_G Cuts at: 1870 4685 1870 Size: 2815 4545 XmnI GAAnn'nnTTC Cuts at: 2462 2534 3193 5489 2462 Size: 72 659 2296 4333 Fragments arranged by size: 4333 2296 659 72 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5168 276 Size: 53 83 186 936 3634 2468 Fragments arranged by size: 3634 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI