Restriction map for
pFN21AB4571
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BbvI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaI BsgI BsiHKAI BsiWI
BsmBI BsoBI BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FbaI FseI FspI HpaI KasI
KpnI KspI MamI MluI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TseI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 1237 CGCG CG^CG
AccIII 1532 TCCGGA T^CCGGA
AcyI 221 GACGCC G[GA]^CG[CT]C
AflIII 962 ACGTGT A^C[GA][CT]GT
BfaI 1603 CTAG C^TAG
BsaAI 961 TACGTG [CT]AC^GT[GA]
BsaHI 221 GACGCC G[GA]^CG[CT]C
BsaWI 1532 TCCGGA [AT]^CCGG[AT]
BseAI 1532 TCCGGA T^CCGGA
BsiEI 261 CGGTCG CG[GA][CT]^CG
BsmAI 1376 GAGAC GTCTC(1/5)
BsmFI 1674 GTCCC GGGAC(10/14)
BsmI 1528 GCATTC GAATGC(1/-1)
Bsp1407I 965 TGTACA T^GTACA
BspEI 1532 TCCGGA T^CCGGA
BsrGI 965 TGTACA T^GTACA
BssSI 1754 CTCGTG CACGAG(-5/-1)
BstUI 1237 CGCG CG^CG
BstXI 1637 CCAATTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 708 AGATCC [GA]^GATC[CT]
DraI 1566 TTTAAA TTT^AAA
DsaI 1331 CCATGG C^C[GA][CT]GG
EcoT22I 1526 ATGCAT ATGCA^T
FauI 281 CCCGC CCCGC(4/6)
Fnu4HI 994 GCGGC GC^[ACGT]GC
HaeII 1050 AGCGCC [GA]GCGC^[CT]
Hin1I 221 GACGCC G[GA]^CG[CT]C
HindIII 1555 AAGCTT A^AGCTT
ItaI 994 GCGGC GC^[ACGT]GC
MaeI 1603 CTAG C^TAG
MfeI 827 CAATTG C^AATTG
MflI 708 AGATCC [GA]^GATC[CT]
MroI 1532 TCCGGA T^CCGGA
MslI 1432 CATCCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 355 CCGCTG C[AC]G^C[GT]G
MunI 827 CAATTG C^AATTG
MvnI 1237 CGCG CG^CG
NcoI 1331 CCATGG C^CATGG
NsiI 1526 ATGCAT ATGCA^T
NspI 1034 ACATGC [GA]CATG^[CT]
PstI 917 CTGCAG CTGCA^G
SalI 263 GTCGAC G^TCGAC
ScaI 1802 AGTACT AGT^ACT
SexAI 636 ACCTGGT A^CC[AT]GGT
SfcI 917 CTGCAG C^T[GA][CT]AG
SspBI 965 TGTACA T^GTACA
XcmI 1344 CCACGATGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 708 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 263 GTCGAC GT^[AC][GT]AC
781 GTCTAC
AvaII 1233 GGACC G^G[AT]CC
1673 GGTCC
BalI 1253 TGGCCA TGG^CCA
1450 TGGCCA
BbsI 869 GAAGAC GAAGAC(2/6)
716 GTCTTC
BmyI 278 GTGCCC G[AGT]GC[ACT]^C
1816 GTGCCC
BpuAI 869 GAAGAC GAAGAC(2/6)
716 GTCTTC
BseRI 731 CTCCTC GAGGAG(10/8)
629 GAGGAG
Bsp1286I 278 GTGCCC G[AGT]GC[ACT]^C
1816 GTGCCC
Cfr13I 1233 GGACC G^G[ACGT]CC
1673 GGTCC
HgaI 221 GACGC GACGC(5/10)
427 GACGC
HincII 263 GTCGAC GT[CT]^[GA]AC
1323 GTTGAC
HindII 263 GTCGAC GT[CT]^[GA]AC
1323 GTTGAC
MaeII 560 ACGT A^CGT
962 ACGT
MluNI 1253 TGGCCA TGG^CCA
1450 TGGCCA
MscI 1253 TGGCCA TGG^CCA
1450 TGGCCA
Sau96I 1233 GGACC G^G[ACGT]CC
1673 GGTCC
SmlI 874 CTCAAG C^T[CT][GA]AG
1017 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BcnI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI Cac8I
CfoI DdeI DpnII EaeI EarI
EcoRII EcoT14I FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
Ksp632I MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI ScrFI SfaNI StyI TaqI
TfiI Tru9I Tsp45I Tsp509I TspRI
TthHB8I