(Circular) (Six-base) MAPSORT of: pFN21AB4571.seq Check: 7109 from: 1 to: 6550 pFN21AB4571 6550 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4360 278 Size: 53 83 186 936 2824 2468 Fragments arranged by size: 2824 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2269 2787 3893 1937 Size: 332 518 1106 4594 Fragments arranged by size: 4594 1106 518 332 Acc65I G'GTAC_C Cuts at: 3871 3871 Size: 6550 AclI AA'CG_TT Cuts at: 1563 4677 5050 1563 Size: 3114 373 3063 Fragments arranged by size: 3114 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2390 2969 4613 5997 6265 1632 Size: 758 579 1644 1384 268 1917 Fragments arranged by size: 1917 1644 1384 758 579 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6353 AflIII A'CryG_T Cuts at: 1203 2967 5449 6426 1203 Size: 1764 2482 977 1327 Fragments arranged by size: 2482 1764 1327 977 AgeI A'CCGG_T Cuts at: 5381 5381 Size: 6550 AhdI GACnn_n'nnGTC Cuts at: 5279 5279 Size: 6550 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6550 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6550 AlwNI CAG_nnn'CTG Cuts at: 4293 5865 4293 Size: 1572 4978 ApaLI G'TGCA_C Cuts at: 4607 5763 4607 Size: 1156 5394 ApoI r'AATT_y Cuts at: 1493 2018 2082 2459 2728 3517 3859 4085 4237 Size: 525 64 377 269 789 342 226 152 Cuts at: 4237 6346 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 789 525 377 342 269 226 152 64 AseI AT'TA_AT Cuts at: 160 1032 5104 160 Size: 872 4072 1606 Fragments arranged by size: 4072 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6550 AvaI C'yCGr_G Cuts at: 1870 1943 3875 1870 Size: 73 1932 4545 Fragments arranged by size: 4545 1932 73 BamHI G'GATC_C Cuts at: 3880 4247 3880 Size: 367 6183 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3871 5326 618 Size: 325 217 627 2084 1455 1842 Fragments arranged by size: 2084 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3869 1391 Size: 2478 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2714 2881 928 Size: 971 815 167 4597 Fragments arranged by size: 4597 971 815 167 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4741 4775 1903 Size: 34 50 34 2720 34 3678 Fragments arranged by size: 3678 2720 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4442 5652 4442 Size: 1210 5340 BclI T'GATC_A Cuts at: 1548 1548 Size: 6550 BfrBI ATG'CAT Cuts at: 3533 3533 Size: 6550 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5161 136 Size: 107 122 71 631 605 3489 1525 Fragments arranged by size: 3489 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6545 6545 Size: 6550 BlpI GC'TnA_GC Cuts at: 3967 3967 Size: 6550 Bme1580I G_kGCm'C Cuts at: 1790 2287 3825 4611 5767 1790 Size: 497 1538 786 1156 2573 Fragments arranged by size: 2573 1538 1156 786 497 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6550 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2712 3543 4281 Size: 416 53 83 399 24 1295 831 738 Cuts at: 4281 5239 442 Size: 958 1753 Fragments arranged by size: 1753 1295 958 831 738 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6550 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5210 1239 Size: 3971 2579 Bpu10I CC'TnA_GC Cuts at: 2412 2412 Size: 6550 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2864 3043 4675 5540 5838 2864 Size: 179 1632 865 298 3576 Fragments arranged by size: 3576 1632 865 298 179 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5213 882 Size: 617 3714 2219 Fragments arranged by size: 3714 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2968 4341 493 Size: 711 1764 1373 2702 Fragments arranged by size: 2702 1764 1373 711 BsaBI GATnn'nnATC Cuts at: 6544 6544 Size: 6550 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2227 4357 4739 5374 Size: 53 83 186 936 694 2130 382 635 Cuts at: 5374 275 Size: 1451 Fragments arranged by size: 2130 1451 936 694 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3537 4983 5381 5655 5802 6213 3537 Size: 1446 398 274 147 411 3874 Fragments arranged by size: 3874 1446 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2087 2117 3372 3402 1178 Size: 30 165 30 684 30 1255 30 4326 Fragments arranged by size: 4326 1255 684 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2649 2727 1186 Size: 1463 78 5009 Fragments arranged by size: 5009 1463 78 BseYI C'CCAG_C Cuts at: 2106 5753 6249 2106 Size: 3647 496 2407 Fragments arranged by size: 3647 2407 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5977 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2269 4000 4761 5789 1540 Size: 275 186 268 1731 761 1028 2301 Fragments arranged by size: 2301 1731 1028 761 275 268 186 BsiHKAI G_wGCw'C Cuts at: 4611 4696 5767 4611 Size: 85 1071 5394 Fragments arranged by size: 5394 1071 85 BsmI GAATG_Cn' Cuts at: 3533 4061 4154 3533 Size: 528 93 5929 Fragments arranged by size: 5929 528 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2287 3825 3869 4611 4696 5767 1391 Size: 399 497 1538 44 742 85 1071 2174 Fragments arranged by size: 2174 1538 1071 742 497 399 85 44 BspEI T'CCGG_A Cuts at: 3537 6213 3537 Size: 2676 3874 BspHI T'CATG_A Cuts at: 4439 6169 4439 Size: 1730 4820 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3905 844 Size: 3061 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4437 1394 Size: 429 2614 3507 Fragments arranged by size: 3507 2614 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5045 5219 65 Size: 1183 3797 174 1396 Fragments arranged by size: 3797 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5194 5381 1700 Size: 110 3384 187 2869 Fragments arranged by size: 3384 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2970 96 Size: 2874 3676 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6550 BssSI C'ACGA_G Cuts at: 3759 4610 5622 3759 Size: 851 1012 4687 Fragments arranged by size: 4687 1012 851 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6550 BstBI TT'CG_AA Cuts at: 5354 5354 Size: 6550 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6550 BstXI CCAn_nnnn'nTGG Cuts at: 3649 3649 Size: 6550 BstYI r'GATC_y Cuts at: 1972 2713 3880 4247 4632 4649 6101 6187 6199 Size: 741 1167 367 385 17 1452 86 12 Cuts at: 6199 6545 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1167 741 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3336 513 Size: 552 2271 3727 Fragments arranged by size: 3727 2271 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5811 Fragments arranged by size: 5811 683 56 BtsI GCAGTG_nn' Cuts at: 2589 4066 4860 4880 2589 Size: 1477 794 20 4259 Fragments arranged by size: 4259 1477 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6550 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6515 DraI TTT'AAA Cuts at: 3573 3854 3573 Size: 281 6269 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5557 809 Size: 507 4241 1802 Fragments arranged by size: 4241 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3000 3115 3258 Size: 54 1425 50 275 18 1170 115 143 Cuts at: 3258 3455 3997 4886 6515 8 Size: 197 542 889 1629 43 Fragments arranged by size: 1629 1425 1170 889 542 275 197 143 115 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3997 1537 Size: 275 2185 4090 Fragments arranged by size: 4090 2185 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2512 2687 2948 4480 1353 Size: 1159 175 261 1532 3423 Fragments arranged by size: 3423 1532 1159 261 175 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5137 5511 5657 387 Size: 1064 138 3548 374 146 1280 Fragments arranged by size: 3548 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2390 2969 4613 5210 5997 6265 1239 Size: 393 758 579 1644 597 787 268 1524 Fragments arranged by size: 1644 1524 787 758 597 579 393 268 EcoRI G'AATT_C Cuts at: 3859 4237 3859 Size: 378 6172 FspI TGC'GCA Cuts at: 5056 5056 Size: 6550 HaeII r_GCGC'y Cuts at: 1678 3059 5697 1678 Size: 1381 2638 2531 Fragments arranged by size: 2638 2531 1381 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2087 2119 2511 2543 3016 Size: 32 135 32 515 32 392 32 473 Cuts at: 3016 3048 5191 5223 5265 5297 1373 Size: 32 2143 32 42 32 2626 Fragments arranged by size: 2626 2143 515 473 392 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2270 3330 3894 4140 669 Size: 988 281 332 1060 564 246 3079 Fragments arranged by size: 3079 1060 988 564 332 281 246 HindIII A'AGCT_T Cuts at: 748 3560 748 Size: 2812 3738 HpaI GTT'AAC Cuts at: 4140 4140 Size: 6550 KpnI G_GTAC'C Cuts at: 3875 3875 Size: 6550 MfeI C'AATT_G Cuts at: 2832 4149 2832 Size: 1317 5233 MluI A'CGCG_T Cuts at: 6426 6426 Size: 6550 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5664 5848 1305 Size: 4359 184 2007 Fragments arranged by size: 4359 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3260 3457 10 Size: 54 1425 343 1428 197 3103 Fragments arranged by size: 3103 1428 1425 343 197 54 MslI CAynn'nnrTG Cuts at: 518 1166 3441 4508 4867 5026 518 Size: 648 2275 1067 359 159 2042 Fragments arranged by size: 2275 2042 1067 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2362 3966 4643 5791 6036 1606 Size: 756 1604 677 1148 245 2120 Fragments arranged by size: 2120 1604 1148 756 677 245 NcoI C'CATG_G Cuts at: 513 1065 3336 513 Size: 552 2271 3727 Fragments arranged by size: 3727 2271 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6550 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6550 NotI GC'GGCC_GC Cuts at: 3997 3997 Size: 6550 NsiI A_TGCA'T Cuts at: 3535 3535 Size: 6550 NspI r_CATG'y Cuts at: 1132 1229 3043 3908 5453 1132 Size: 97 1814 865 1545 2229 Fragments arranged by size: 2229 1814 1545 865 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6550 PciI A'CATG_T Cuts at: 5449 5449 Size: 6550 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6550 PmeI GTTT'AAAC Cuts at: 3854 3854 Size: 6550 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4619 4651 6132 6164 4619 Size: 32 1481 32 5005 Fragments arranged by size: 5005 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6550 PsiI TTA'TAA Cuts at: 4120 4120 Size: 6550 PstI C_TGCA'G Cuts at: 830 2926 3902 830 Size: 2096 976 3478 Fragments arranged by size: 3478 2096 976 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6550 SalI G'TCGA_C Cuts at: 1936 2268 3892 1936 Size: 332 1624 4594 Fragments arranged by size: 4594 1624 332 SbfI CC_TGCA'GG Cuts at: 3902 3902 Size: 6550 ScaI AGT'ACT Cuts at: 3809 4798 6510 3809 Size: 989 1712 3849 Fragments arranged by size: 3849 1712 989 SexAI A'CCwGG_T Cuts at: 2641 2641 Size: 6550 SfcI C'TryA_G Cuts at: 826 1045 2922 3898 5033 5714 5905 826 Size: 219 1877 976 1135 681 191 1471 Fragments arranged by size: 1877 1471 1135 976 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6550 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6550 SmaI CCC'GGG Cuts at: 1872 3877 1872 Size: 2005 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2879 3022 4654 5555 5817 820 Size: 197 926 936 143 1632 901 262 1553 Fragments arranged by size: 1632 1553 936 926 901 262 197 143 SnaBI TAC'GTA Cuts at: 493 4341 493 Size: 3848 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6550 SphI G_CATG'C Cuts at: 1132 3908 1132 Size: 2776 3774 SspI AAT'ATT Cuts at: 5 52 4474 5 Size: 47 4422 2081 Fragments arranged by size: 4422 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6550 StyI C'CwwG_G Cuts at: 513 1065 3255 3336 3669 3989 513 Size: 552 2190 81 333 320 3074 Fragments arranged by size: 3074 2190 552 333 320 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3181 3641 4577 4594 4747 4932 5377 Size: 1255 1601 460 936 17 153 185 445 Cuts at: 5377 325 Size: 1498 Fragments arranged by size: 1601 1498 1255 936 460 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2970 3807 4796 Size: 274 80 33 51 1443 993 837 989 Cuts at: 4796 6508 96 Size: 1712 138 Fragments arranged by size: 1712 1443 993 989 837 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3372 3404 6510 6542 3372 Size: 32 3106 32 3380 Fragments arranged by size: 3380 3106 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6550 XbaI T'CTAG_A Cuts at: 3886 3886 Size: 6550 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3356 3356 Size: 6550 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6550 XmaI C'CCGG_G Cuts at: 1870 3875 1870 Size: 2005 4545 XmnI GAAnn'nnTTC Cuts at: 4679 4679 Size: 6550 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4358 276 Size: 53 83 186 936 2824 2468 Fragments arranged by size: 2824 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI