Restriction map for
pFN21AB4495
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbeI BbsI BciVI BclI
BfrI BlnI BpuAI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BsrDI BssHII Bst1107I BstEII
BstPI BstUI BstZ17I ClaI CpoI
DraI DrdI EagI Eam1105I EarI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MfeI MluI MroI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 142 GTAGAC GT^[AC][GT]AC
AcsI 2245 AAATTC [GA]^AATT[CT]
Alw44I 2140 GTGCAC G^TGCAC
AocI 1245 CCTGAGG CC^T[ACGT]AGG
ApaLI 2140 GTGCAC G^TGCAC
ApoI 2245 AAATTC [GA]^AATT[CT]
Asp700I 2451 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1306 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 14 GGATCC G^GATCC
BanI 1536 GGCACC G^G[CT][GA]CC
BbrPI 1018 CACGTG CAC^GTG
BbvCI 1825 CCTCAGC CCTCAGC(-5/-2)
BcgI 641 GCAACATCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 2404 CTAG C^TAG
BglII 1058 AGATCT A^GATCT
BsaBI 180 GATTGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 341 ACCGGA [AT]^CCGG[AT]
BsmBI 94 GAGACG CGTCTC(1/5)
Bsp1407I 464 TGTACA T^GTACA
BsrGI 464 TGTACA T^GTACA
BssSI 2137 CTCGTG CACGAG(-5/-1)
BstAPI 2226 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 848 TTCGAA TT^CGAA
Bsu36I 1245 CCTGAGG CC^T[ACGT]AGG
Eco81I 1245 CCTGAGG CC^T[ACGT]AGG
EcoNI 2267 CCTCCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeI 2404 CTAG C^TAG
MamI 180 GATTGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 104 TTAA T^TAA
NcoI 1365 CCATGG C^CATGG
NdeI 1271 CATATG CA^TATG
NspV 848 TTCGAA TT^CGAA
PflFI 1306 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1018 CACGTG CAC^GTG
PmlI 1018 CACGTG CAC^GTG
ScaI 562 AGTACT AGT^ACT
SfuI 848 TTCGAA TT^CGAA
SspBI 464 TGTACA T^GTACA
Tru9I 104 TTAA T^TAA
Tth111I 1306 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 2451 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 8 enzymes) #
###################################
ApaI 2171 GGGCCC GGGCC^C
2193 GGGCCC
BalI 909 TGGCCA TGG^CCA
2591 TGGCCA
BsrFI 690 GCCGGT [GA]^CCGG[CT]
799 ACCGGC
Cfr10I 690 GCCGGT [GA]^CCGG[CT]
799 ACCGGC
DraIII 353 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1392 CACCGTGTG
MluNI 909 TGGCCA TGG^CCA
2591 TGGCCA
MscI 909 TGGCCA TGG^CCA
2591 TGGCCA
SacI 1093 GAGCTC GAGCT^C
1747 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI Aor51HI AspHI AvaI AvaII
BanII BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eco47III EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII