Restriction map for
pFN21AB4447
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AgeI Aor51HI
ApaI ApoI AscI AseI AsnI
AviII AvrII BamHI BbeI BcgI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII ClaI CpoI DraI DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HgaI Hin1I HindIII HinfI HpaI
KasI KspI MamI MfeI MluI
MroI MslI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PleI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tsp509I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 766 GGTACC G^GTACC
AflIII 693 ACACGT A^C[GA][CT]GT
AhdI 201 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 48 GTGCAC G^TGCAC
AocI 32 CCTCAGG CC^T[ACGT]AGG
ApaLI 48 GTGCAC G^TGCAC
Asp700I 790 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 766 GGTACC G^GTACC
AspEI 201 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 520 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 694 CACGTG CAC^GTG
BbsI 742 GAAGAC GAAGAC(2/6)
BbvCI 601 GCTGAGG CCTCAGC(-5/-2)
BciVI 895 GTATCC GTATCC(6/5)
BclI 165 TGATCA T^GATCA
BfaI 188 CTAG C^TAG
BpuAI 742 GAAGAC GAAGAC(2/6)
BsaAI 694 CACGTG [CT]AC^GT[GA]
BseRI 139 CTCCTC GAGGAG(10/8)
BsmI 1231 GCATTC GAATGC(1/-1)
BsrDI 169 CATTGC GCAATG(2/0)
BsrFI 566 GCCGGT [GA]^CCGG[CT]
BssSI 466 CTCGTG CACGAG(-5/-1)
BstAPI 3 GCAGGGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 32 CCTCAGG CC^T[ACGT]AGG
Cfr10I 566 GCCGGT [GA]^CCGG[CT]
DraIII 694 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 201 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 541 CTCTTC CTCTTC(1/4)
Eco81I 32 CCTCAGG CC^T[ACGT]AGG
EcoT14I 864 CCATGG C^C[AT][AT]GG
FauI 558 GCGGG CCCGC(4/6)
FbaI 165 TGATCA T^GATCA
HincII 526 GTCAAC GT[CT]^[GA]AC
HindII 526 GTCAAC GT[CT]^[GA]AC
HphI 1157 TCACC GGTGA(8/7)
KpnI 766 GGTACC GGTAC^C
Ksp632I 541 CTCTTC CTCTTC(1/4)
MaeI 188 CTAG C^TAG
NcoI 864 CCATGG C^CATGG
PflFI 520 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 694 CACGTG CAC^GTG
PmlI 694 CACGTG CAC^GTG
PstI 553 CTGCAG CTGCA^G
PvuII 194 CAGCTG CAG^CTG
SacI 538 GAGCTC GAGCT^C
SapI 540 GCTCTTC GCTCTTC(1/4)
ScaI 776 AGTACT AGT^ACT
SfaNI 858 GATGC GCATC(5/9)
SmaI 581 CCCGGG CCC^GGG
StyI 864 CCATGG C^C[AT][AT]GG
Tth111I 520 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 581 CCCGGG C^CCGGG
XmaI 581 CCCGGG C^CCGGG
XmnI 790 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 936 GATCC GGATC(4/5)
654 GGATC
AlwNI 1003 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1121 CAGGACCTG
AvaI 18 CTCGGG C^[CT]CG[GA]G
581 CCCGGG
BpmI 219 CTCCAG CTGGAG(16/14)
574 CTGGAG
BsgI 628 CTGCAC GTGCAG(16/14)
919 CTGCAC
BsoBI 18 CTCGGG C^[CT]CG[GA]G
581 CCCGGG
BstYI 654 GGATCT [GA]^GATC[CT]
935 AGATCC
DsaI 275 CCACGG C^C[GA][CT]GG
864 CCATGG
EcoNI 17 CCTCGGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
717 CCTCTGCAAGG
HaeII 563 AGCGCC [GA]GCGC^[CT]
613 AGCGCC
MaeII 449 ACGT A^CGT
695 ACGT
MaeIII 965 GTGAC ^GT[ACGT]AC
1117 GTGAC
MflI 654 GGATCT [GA]^GATC[CT]
935 AGATCC
MseI 394 TTAA T^TAA
1037 TTAA
PflMI 414 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1061 CCATAAACTGG
SmlI 1030 CTTGAG C^T[CT][GA]AG
1224 CTCAAG
Tru9I 394 TTAA T^TAA
1037 TTAA
Tsp45I 965 GTGAC ^GT[GC]AC
1117 GTGAC
Van91I 414 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1061 CCATAAACTGG
XhoII 654 GGATCT [GA]^GATC[CT]
935 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BalI BanI BanII BbvI BcnI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MboI
MboII MluNI MnlI MscI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PpuMI RsaI Sau3AI
Sau96I ScrFI SfcI TaqI TseI
TspRI TthHB8I