(Circular) (Six-base) MAPSORT of: pFN21AB4447.seq Check: 2772 from: 1 to: 5986 pFN21AB4447 5986 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2040 2565 2040 Size: 525 5461 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3796 278 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3329 1937 Size: 1392 4594 Acc65I G'GTAC_C Cuts at: 2771 3307 2771 Size: 536 5450 AclI AA'CG_TT Cuts at: 1563 4113 4486 1563 Size: 2550 373 3063 Fragments arranged by size: 3063 2550 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2111 2241 2468 2766 2814 3043 4049 5433 Size: 479 130 227 298 48 229 1006 1384 Cuts at: 5433 5701 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1006 479 298 268 229 227 130 48 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5789 AflIII A'CryG_T Cuts at: 1203 2698 4885 5862 1203 Size: 1495 2187 977 1327 Fragments arranged by size: 2187 1495 1327 977 AgeI A'CCGG_T Cuts at: 4817 4817 Size: 5986 AhdI GACnn_n'nnGTC Cuts at: 2211 4715 2211 Size: 2504 3482 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5986 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5986 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2558 2590 2558 Size: 32 5954 AlwNI CAG_nnn'CTG Cuts at: 3013 3131 3729 5301 3013 Size: 118 598 1572 3698 Fragments arranged by size: 3698 1572 598 118 ApaLI G'TGCA_C Cuts at: 2053 4043 5199 2053 Size: 1990 1156 2840 Fragments arranged by size: 2840 1990 1156 ApoI r'AATT_y Cuts at: 1493 3295 3521 3673 5782 1493 Size: 1802 226 152 2109 1697 Fragments arranged by size: 2109 1802 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4540 160 Size: 872 3508 1606 Fragments arranged by size: 3508 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 AvaI C'yCGr_G Cuts at: 1870 1943 2023 2586 3311 1870 Size: 73 80 563 725 4545 Fragments arranged by size: 4545 725 563 80 73 BamHI G'GATC_C Cuts at: 3316 3683 3316 Size: 367 5619 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2085 2460 2771 2788 3307 Size: 325 217 627 298 375 311 17 519 Cuts at: 3307 4762 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 519 375 325 311 298 217 17 BanII G_rGCy'C Cuts at: 1391 2547 2586 3020 3305 1391 Size: 1156 39 434 285 4072 Fragments arranged by size: 4072 1156 434 285 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2754 928 Size: 971 855 4160 Fragments arranged by size: 4160 971 855 BbvCI CC'TCA_GC Cuts at: 2607 2607 Size: 5986 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4177 4211 1903 Size: 34 50 34 2156 34 3678 Fragments arranged by size: 3678 2156 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2911 3878 5088 2911 Size: 967 1210 3809 Fragments arranged by size: 3809 1210 967 BclI T'GATC_A Cuts at: 1548 2170 1548 Size: 622 5364 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4597 136 Size: 107 122 71 631 605 2925 1525 Fragments arranged by size: 2925 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5981 5981 Size: 5986 BlpI GC'TnA_GC Cuts at: 3403 3403 Size: 5986 Bme1580I G_kGCm'C Cuts at: 1790 2057 4047 5203 1790 Size: 267 1990 1156 2573 Fragments arranged by size: 2573 1990 1156 267 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5986 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3082 3717 4675 Size: 416 53 83 399 24 1665 635 958 Cuts at: 4675 442 Size: 1753 Fragments arranged by size: 1753 1665 958 635 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5986 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3117 3117 Size: 5986 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2209 2600 4646 1239 Size: 970 391 2046 2579 Fragments arranged by size: 2579 2046 970 391 Bpu10I CC'TnA_GC Cuts at: 2607 2607 Size: 5986 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3056 3214 4111 4976 5274 3056 Size: 158 897 865 298 3768 Fragments arranged by size: 3768 897 865 298 158 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4649 882 Size: 617 3150 2219 Fragments arranged by size: 3150 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2701 3777 493 Size: 711 1497 1076 2702 Fragments arranged by size: 2702 1497 1076 711 BsaBI GATnn'nnATC Cuts at: 5980 5980 Size: 5986 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3793 4175 4810 275 Size: 53 83 186 936 2260 382 635 1451 Fragments arranged by size: 2260 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4419 4817 5091 5238 5649 4419 Size: 398 274 147 411 4756 Fragments arranged by size: 4756 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2558 2588 2621 2651 1178 Size: 30 165 30 1155 30 33 30 4513 Fragments arranged by size: 4513 1155 165 33 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 1186 Size: 949 5037 BseYI C'CCAG_C Cuts at: 2197 2201 2274 3013 5189 5685 2197 Size: 4 73 739 2176 496 2498 Fragments arranged by size: 2498 2176 739 496 73 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2618 2909 1163 Size: 573 882 291 4240 Fragments arranged by size: 4240 882 573 291 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3436 4197 5225 1540 Size: 275 186 1435 761 1028 2301 Fragments arranged by size: 2301 1435 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2057 2523 2547 4047 4132 5203 2057 Size: 466 24 1500 85 1071 2840 Fragments arranged by size: 2840 1500 1071 466 85 24 BsmI GAATG_Cn' Cuts at: 3236 3497 3590 3236 Size: 261 93 5632 Fragments arranged by size: 5632 261 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2523 2547 2586 3020 3305 4047 Size: 399 267 466 24 39 434 285 742 Cuts at: 4047 4132 5203 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 466 434 399 285 267 85 39 24 BspEI T'CCGG_A Cuts at: 5649 5649 Size: 5986 BspHI T'CATG_A Cuts at: 3875 5605 3875 Size: 1730 4256 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2040 2565 2640 3139 3341 844 Size: 1196 525 75 499 202 3489 Fragments arranged by size: 3489 1196 525 499 202 75 BsrBI CCG'CTC Cuts at: 1394 1823 3873 1394 Size: 429 2050 3507 Fragments arranged by size: 3507 2050 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2173 4481 4655 65 Size: 1183 925 2308 174 1396 Fragments arranged by size: 2308 1396 1183 925 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2571 4630 4817 1700 Size: 110 761 2059 187 2869 Fragments arranged by size: 2869 2059 761 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5986 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5986 BssSI C'ACGA_G Cuts at: 2471 4046 5058 2471 Size: 1575 1012 3399 Fragments arranged by size: 3399 1575 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2014 794 Size: 1220 4766 BstBI TT'CG_AA Cuts at: 4790 4790 Size: 5986 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5986 BstYI r'GATC_y Cuts at: 1972 2659 2940 3316 3683 4068 4085 5537 5623 Size: 687 281 376 367 385 17 1452 86 Cuts at: 5623 5635 5981 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 687 385 376 367 346 281 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2038 2038 Size: 5986 BtgI C'CryG_G Cuts at: 513 1065 2280 2869 513 Size: 552 1215 589 3630 Fragments arranged by size: 3630 1215 589 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5247 Fragments arranged by size: 5247 683 56 BtsI GCAGTG_nn' Cuts at: 2087 2376 2443 2832 3502 4296 4316 2087 Size: 289 67 389 670 794 20 3757 Fragments arranged by size: 3757 794 670 389 289 67 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5986 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5951 DraI TTT'AAA Cuts at: 3290 3290 Size: 5986 DraIII CAC_nnn'GTG Cuts at: 2704 2704 Size: 5986 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4993 809 Size: 507 3677 1802 Fragments arranged by size: 3677 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2337 2613 2961 Size: 54 1425 50 275 18 507 276 348 Cuts at: 2961 3210 3433 4322 5951 8 Size: 249 223 889 1629 43 Fragments arranged by size: 1629 1425 889 507 348 276 275 249 223 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3433 1537 Size: 275 1621 4090 Fragments arranged by size: 4090 1621 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2552 3916 1353 Size: 1199 1364 3423 Fragments arranged by size: 3423 1364 1199 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2121 4573 4947 5093 387 Size: 1064 138 532 2452 374 146 1280 Fragments arranged by size: 2452 1280 1064 532 374 146 138 EcoICRI GAG'CTC Cuts at: 2545 2545 Size: 5986 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2209 2241 2468 2600 2766 2814 Size: 393 479 98 32 227 132 166 48 Cuts at: 2814 3043 4049 4646 5433 5701 1239 Size: 229 1006 597 787 268 1524 Fragments arranged by size: 1524 1006 787 597 479 393 268 229 227 166 132 98 48 32 EcoNI CCTnn'n_nnAGG Cuts at: 2026 2726 2026 Size: 700 5286 EcoO109I rG'GnC_Cy Cuts at: 2205 2252 2329 3128 3225 2205 Size: 47 77 799 97 4966 Fragments arranged by size: 4966 799 97 77 47 EcoRI G'AATT_C Cuts at: 3295 3673 3295 Size: 378 5608 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2413 2530 2413 Size: 117 5869 FspI TGC'GCA Cuts at: 4492 4492 Size: 5986 HaeII r_GCGC'y Cuts at: 1678 2572 2622 5133 1678 Size: 894 50 2511 2531 Fragments arranged by size: 2531 2511 894 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2197 2229 2741 2773 4627 Size: 32 135 32 625 32 512 32 1854 Cuts at: 4627 4659 4701 4733 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1854 625 512 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2533 3330 3576 669 Size: 988 281 595 797 246 3079 Fragments arranged by size: 3079 988 797 595 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5986 HpaI GTT'AAC Cuts at: 3576 3576 Size: 5986 KpnI G_GTAC'C Cuts at: 2775 3311 2775 Size: 536 5450 MfeI C'AATT_G Cuts at: 3585 3585 Size: 5986 MluI A'CGCG_T Cuts at: 5862 5862 Size: 5986 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5100 5284 1305 Size: 3795 184 2007 Fragments arranged by size: 3795 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2339 2615 3212 10 Size: 54 1425 343 507 276 597 2784 Fragments arranged by size: 2784 1425 597 507 343 276 54 MslI CAynn'nnrTG Cuts at: 518 1166 3944 4303 4462 518 Size: 648 2778 359 159 2042 Fragments arranged by size: 2778 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2201 2563 3010 3402 4079 5227 5472 1606 Size: 595 362 447 392 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 595 447 392 362 245 NcoI C'CATG_G Cuts at: 513 1065 2869 513 Size: 552 1804 3630 Fragments arranged by size: 3630 1804 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5986 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5986 NotI GC'GGCC_GC Cuts at: 3433 3433 Size: 5986 NspI r_CATG'y Cuts at: 1132 1229 3344 4889 1132 Size: 97 2115 1545 2229 Fragments arranged by size: 2229 2115 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5986 PciI A'CATG_T Cuts at: 4885 4885 Size: 5986 PflMI CCAn_nnn'nTGG Cuts at: 1108 2425 3072 1108 Size: 1317 647 4022 Fragments arranged by size: 4022 1317 647 PfoI T'CCnGG_A Cuts at: 2577 2577 Size: 5986 PmeI GTTT'AAAC Cuts at: 3290 3290 Size: 5986 PmlI CAC'GTG Cuts at: 2701 2701 Size: 5986 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2558 2590 4055 4087 5568 5600 2558 Size: 32 1465 32 1481 32 2944 Fragments arranged by size: 2944 1481 1465 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2205 2329 3128 2205 Size: 124 799 5063 Fragments arranged by size: 5063 799 124 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5986 PsiI TTA'TAA Cuts at: 3556 3556 Size: 5986 PstI C_TGCA'G Cuts at: 830 2562 3338 830 Size: 1732 776 3478 Fragments arranged by size: 3478 1732 776 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5986 PvuII CAG'CTG Cuts at: 2201 2201 Size: 5986 SacI G_AGCT'C Cuts at: 2547 2547 Size: 5986 SalI G'TCGA_C Cuts at: 1936 3328 1936 Size: 1392 4594 SanDI GG'GwC_CC Cuts at: 2205 2205 Size: 5986 SapI GCTCTTCn'nnn_ Cuts at: 2552 2552 Size: 5986 SbfI CC_TGCA'GG Cuts at: 3338 3338 Size: 5986 ScaI AGT'ACT Cuts at: 2783 4234 5946 2783 Size: 1451 1712 2823 Fragments arranged by size: 2823 1712 1451 SfcI C'TryA_G Cuts at: 826 1045 2179 2381 2558 2887 2936 3334 4469 Size: 219 1134 202 177 329 49 398 1135 Cuts at: 4469 5150 5341 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1134 681 398 329 219 202 191 177 49 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5986 SmaI CCC'GGG Cuts at: 1872 2588 3313 1872 Size: 716 725 4545 Fragments arranged by size: 4545 725 716 SmlI C'TyrA_G Cuts at: 820 1017 1943 3035 3229 4090 4991 5253 820 Size: 197 926 1092 194 861 901 262 1553 Fragments arranged by size: 1553 1092 926 901 861 262 197 194 SnaBI TAC'GTA Cuts at: 493 3777 493 Size: 3284 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5986 SphI G_CATG'C Cuts at: 1132 3344 1132 Size: 2212 3774 SspI AAT'ATT Cuts at: 5 52 3910 5 Size: 47 3858 2081 Fragments arranged by size: 3858 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5986 StyI C'CwwG_G Cuts at: 513 1065 2869 3425 513 Size: 552 1804 556 3074 Fragments arranged by size: 3074 1804 556 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3179 4013 4030 4183 4368 4813 325 Size: 1255 1599 834 17 153 185 445 1498 Fragments arranged by size: 1599 1498 1255 834 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2781 4232 5944 Size: 274 80 33 51 1443 804 1451 1712 Cuts at: 5944 96 Size: 138 Fragments arranged by size: 1712 1451 1443 804 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2688 2720 2822 2854 5946 5978 2688 Size: 32 102 32 3092 32 2696 Fragments arranged by size: 3092 2696 102 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2528 1316 Size: 1212 4774 XbaI T'CTAG_A Cuts at: 3322 3322 Size: 5986 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5986 XmaI C'CCGG_G Cuts at: 1870 2586 3311 1870 Size: 716 725 4545 Fragments arranged by size: 4545 725 716 XmnI GAAnn'nnTTC Cuts at: 2799 4115 2799 Size: 1316 4670 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3794 276 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI