Restriction map for
pFN21AB4431
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# NO CUT ENZYMES ( 73 enzymes) #
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AatII AclI AcsI AflII AgeI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp700I AviII AvrII BbeI
BbsI BcgI BciVI BclI BfrI
BlnI BpuAI BsaBI BsmI BspDI
BspLU11I BssHII BssSI ClaI CpoI
DraIII EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KasI MamI MfeI MluI MunI
NarI NheI NotI NruI NsiI
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
SapI SbfI ScaI SfoI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 1287 GGTACC G^GTACC
AccI 2124 GTATAC GT^[AC][GT]AC
AhdI 948 GACCAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1287 GGTACC G^GTACC
AspEI 948 GACCAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1739 TGGCCA TGG^CCA
BamHI 1152 GGATCC G^GATCC
BlpI 2569 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2569 GCTGAGC GC^T[ACGT]AGC
BsiWI 1173 CGTACG C^GTACG
BsrBI 548 CCGCTC CCGCTC(-3/-3)
Bst1107I 2124 GTATAC GTA^TAC
BstAPI 667 GCACTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1352 TTCGAA TT^CGAA
BstZ17I 2124 GTATAC GTA^TAC
CelII 2569 GCTGAGC GC^T[ACGT]AGC
DraI 567 TTTAAA TTT^AAA
DrdI 1208 GACCCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 948 GACCAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1048 CCTTTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 703 AAGCTT A^AGCTT
KpnI 1287 GGTACC GGTAC^C
KspI 61 CCGCGG CCGC^GG
MluNI 1739 TGGCCA TGG^CCA
MscI 1739 TGGCCA TGG^CCA
NaeI 334 GCCGGC GCC^GGC
NcoI 1796 CCATGG C^CATGG
NdeI 2195 CATATG CA^TATG
NgoMIV 334 GCCGGC G^CCGGC
NspV 1352 TTCGAA TT^CGAA
SacII 61 CCGCGG CCGC^GG
SexAI 860 ACCTGGT A^CC[AT]GGT
SfiI 1252 GGCCAGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1352 TTCGAA TT^CGAA
SmaI 1406 CCCGGG CCC^GGG
SmlI 1674 CTCAAG C^T[CT][GA]AG
TfiI 1003 GATTC G^A[AT]TC
XcmI 351 CCAGGTGGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1406 CCCGGG C^CCGGG
XmaI 1406 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 46 enzymes) #
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AccII 62 CGCG CG^CG
1373 CGCG
AccIII 1763 TCCGGA T^CCGGA
2262 TCCGGA
AcyI 331 GACGCC G[GA]^CG[CT]C
2190 GACGCC
AocI 885 CCTCAGG CC^T[ACGT]AGG
1695 CCTCAGG
Aor51HI 323 AGCGCT AGC^GCT
970 AGCGCT
ApaI 913 GGGCCC GGGCC^C
1260 GGGCCC
AspI 156 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1393 GACAGTGTC
BbrPI 1735 CACGTG CAC^GTG
2180 CACGTG
BfaI 722 CTAG C^TAG
958 CTAG
BsaHI 331 GACGCC G[GA]^CG[CT]C
2190 GACGCC
BsaI 1961 GAGACC GGTCTC(1/5)
1230 GGTCTC
BseAI 1763 TCCGGA T^CCGGA
2262 TCCGGA
BsgI 1247 GTGCAG GTGCAG(16/14)
2131 GTGCAG
BsiEI 25 CGGCCG CG[GA][CT]^CG
336 CGGCCG
Bsp1407I 266 TGTACA T^GTACA
772 TGTACA
BspEI 1763 TCCGGA T^CCGGA
2262 TCCGGA
BsrDI 1668 CATTGC GCAATG(2/0)
1914 CATTGC
BsrGI 266 TGTACA T^GTACA
772 TGTACA
BstEII 582 GGTGACC G^GT[ACGT]ACC
2157 GGTGACC
BstPI 582 GGTGACC G^GT[ACGT]ACC
2157 GGTGACC
BstUI 62 CGCG CG^CG
1373 CGCG
Bsu36I 885 CCTCAGG CC^T[ACGT]AGG
1695 CCTCAGG
DsaI 61 CCGCGG C^C[GA][CT]GG
1796 CCATGG
EagI 25 CGGCCG C^GGCCG
336 CGGCCG
EclXI 25 CGGCCG C^GGCCG
336 CGGCCG
Eco47III 323 AGCGCT AGC^GCT
970 AGCGCT
Eco52I 25 CGGCCG C^GGCCG
336 CGGCCG
Eco81I 885 CCTCAGG CC^T[ACGT]AGG
1695 CCTCAGG
EcoO65I 582 GGTGACC G^GT[ACGT]ACC
2157 GGTGACC
Hin1I 331 GACGCC G[GA]^CG[CT]C
2190 GACGCC
MaeI 722 CTAG C^TAG
958 CTAG
MroI 1763 TCCGGA T^CCGGA
2262 TCCGGA
MseI 568 TTAA T^TAA
1874 TTAA
MvnI 62 CGCG CG^CG
1373 CGCG
NspI 1833 GCATGC [GA]CATG^[CT]
1982 GCATGC
PflFI 156 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1393 GACAGTGTC
PmaCI 1735 CACGTG CAC^GTG
2180 CACGTG
PmlI 1735 CACGTG CAC^GTG
2180 CACGTG
PvuII 1606 CAGCTG CAG^CTG
2374 CAGCTG
SacI 755 GAGCTC GAGCT^C
1938 GAGCTC
SphI 1833 GCATGC GCATG^C
1982 GCATGC
SspBI 266 TGTACA T^GTACA
772 TGTACA
StuI 627 AGGCCT AGG^CCT
1008 AGGCCT
Tru9I 568 TTAA T^TAA
1874 TTAA
Tsp509I 1138 AATT ^AATT
1978 AATT
Tth111I 156 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1393 GACAGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BglI
BglII BmyI BpmI BsaAI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrFI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XhoII