Restriction map for
pFN21AB4431
(Circular) (Six-base) MAPSORT of: pFN21AB4431.seq Check: 9349 from: 1 to: 7375
pFN21AB4431 7375 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5185 278
Size: 53 83 186 936 3649 2468
Fragments arranged by size:
3649 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4130 4718 1937
Size: 2193 588 4594
Fragments arranged by size:
4594 2193 588
Acc65I G'GTAC_C
Cuts at: 3292 4696 3292
Size: 1404 5971
AclI AA'CG_TT
Cuts at: 1563 5502 5875 1563
Size: 3939 373 3063
Fragments arranged by size:
3939 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2153 2336 3635 5438 6822 7090 1632
Size: 521 183 1299 1803 1384 268 1917
Fragments arranged by size:
1917 1803 1384 1299 521 268 183
AfeI AGC'GCT
Cuts at: 2330 2977 2330
Size: 647 6728
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7178
AflIII A'CryG_T
Cuts at: 1203 3181 3739 4146 4184 4284 6274 7251 1203
Size: 1978 558 407 38 100 1990 977 1327
Fragments arranged by size:
1990 1978 1327 977 558 407 100 38
AgeI A'CCGG_T
Cuts at: 6206 6206
Size: 7375
AhdI GACnn_n'nnGTC
Cuts at: 2958 6104 2958
Size: 3146 4229
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7375
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7375
AlwNI CAG_nnn'CTG
Cuts at: 2657 2750 3229 3898 4542 4671 5118 6690 2657
Size: 93 479 669 644 129 447 1572 3342
Fragments arranged by size:
3342 1572 669 644 479 447 129 93
ApaI G_GGCC'C
Cuts at: 2922 3269 2922
Size: 347 7028
ApaLI G'TGCA_C
Cuts at: 5432 6588 5432
Size: 1156 6219
ApoI r'AATT_y
Cuts at: 1493 4684 4910 5062 7171 1493
Size: 3191 226 152 2109 1697
Fragments arranged by size:
3191 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5929 160
Size: 872 4897 1606
Fragments arranged by size:
4897 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7375
AvaI C'yCGr_G
Cuts at: 1870 1943 2072 3044 3411 3467 4333 4700 1870
Size: 73 129 972 367 56 866 367 4545
Fragments arranged by size:
4545 972 866 367 367 129 73 56
BamHI G'GATC_C
Cuts at: 3157 4705 5072 3157
Size: 1548 367 5460
Fragments arranged by size:
5460 1548 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2352 2869 2941 3292 4696
Size: 325 217 627 565 517 72 351 1404
Cuts at: 4696 6151 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1404 627 565 517 351 325
217 72
BanII G_rGCy'C
Cuts at: 1391 2040 2149 2764 2922 3269 3947 4443 4694
Size: 649 109 615 158 347 678 496 251
Cuts at: 4694 1391
Size: 4072
Fragments arranged by size:
4072 678 649 615 496 347 251 158
109
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6404
BbvCI CC'TCA_GC
Cuts at: 2012 2309 4434 2012
Size: 297 2125 4953
Fragments arranged by size:
4953 2125 297
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5566 5600 1903
Size: 34 3629 34 3678
Fragments arranged by size:
3678 3629 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5267 6477 5267
Size: 1210 6165
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7375
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2068 2398 3264
Size: 107 122 71 631 605 396 330 866
Cuts at: 3264 5986 136
Size: 2722 1525
Fragments arranged by size:
2722 1525 866 631 605 396 330 122
107 71
BglII A'GATC_T
Cuts at: 2382 2443 4041 7370 2382
Size: 61 1598 3329 2387
Fragments arranged by size:
3329 2387 1598 61
BlpI GC'TnA_GC
Cuts at: 4575 4792 4575
Size: 217 7158
Bme1580I G_kGCm'C
Cuts at: 1790 2841 2922 2946 3269 5436 6592 1790
Size: 1051 81 24 323 2167 1156 2573
Fragments arranged by size:
2573 2167 1156 1051 323 81 24
BmgBI CAC'GTC
Cuts at: 1318 4147 4285 1318
Size: 2829 138 4408
Fragments arranged by size:
4408 2829 138
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2273 2947 4530
Size: 416 53 83 399 24 856 674 1583
Cuts at: 4530 5106 6064 442
Size: 576 958 1753
Fragments arranged by size:
1753 1583 958 856 674 576 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7375
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3646 3646
Size: 7375
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3238 3548 3875 4127 4330 4382 4481 6035
Size: 1999 310 327 252 203 52 99 1554
Cuts at: 6035 1239
Size: 2579
Fragments arranged by size:
2579 1999 1554 327 310 252 203 99
52
Bpu10I CC'TnA_GC
Cuts at: 2012 2309 3941 4434 2012
Size: 297 1632 493 4953
Fragments arranged by size:
4953 1632 493 297
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3664 5500 6365 6663 3664
Size: 1836 865 298 4376
Fragments arranged by size:
4376 1836 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3241 3960 6038 882
Size: 617 1742 719 2078 2219
Fragments arranged by size:
2219 2078 1742 719 617
BsaAI yAC'GTr
Cuts at: 493 1204 3182 3742 4187 5166 493
Size: 711 1978 560 445 979 2702
Fragments arranged by size:
2702 1978 979 711 560 445
BsaBI GATnn'nnATC
Cuts at: 7369 7369
Size: 7375
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2337 4196 5182 5564
Size: 53 83 186 936 804 1859 986 382
Cuts at: 5564 6199 275
Size: 635 1451
Fragments arranged by size:
1859 1451 986 936 804 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3768 3903 4267 5808 6206 6480 6627 7038 3768
Size: 135 364 1541 398 274 147 411 4105
Fragments arranged by size:
4105 1541 411 398 364 274 147 135
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2136 2166 4271 4301 1178
Size: 30 165 30 733 30 2105 30 4252
Fragments arranged by size:
4252 2105 733 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2228 3261 4451 1186
Size: 1042 1033 1190 4110
Fragments arranged by size:
4110 1190 1042 1033
BseYI C'CCAG_C
Cuts at: 2388 2808 2968 3153 3609 4496 6578 7074 2388
Size: 420 160 185 456 887 2082 496 2689
Fragments arranged by size:
2689 2082 887 496 456 420 185 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3273 4157 1163
Size: 573 1537 884 4381
Fragments arranged by size:
4381 1537 884 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2033 2344 4825 5586 6614 1540
Size: 275 186 32 311 2481 761 1028 2301
Fragments arranged by size:
2481 2301 1028 761 311 275 186 32
BsiHKAI G_wGCw'C
Cuts at: 2600 2764 3540 3947 4599 5436 5521 6592 2600
Size: 164 776 407 652 837 85 1071 3383
Fragments arranged by size:
3383 1071 837 776 652 407 164 85
BsiWI C'GTAC_G
Cuts at: 3178 3178
Size: 7375
BsmI GAATG_Cn'
Cuts at: 4886 4979 4886
Size: 93 7282
BsmBI CGTCTCn'nnnn_
Cuts at: 3196 3358 3368 3794 3196
Size: 162 10 426 6777
Fragments arranged by size:
6777 426 162 10
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2149 2600 2764 2841 2922 2946
Size: 399 250 109 451 164 77 81 24
Cuts at: 2946 3269 3540 3947 4443 4599 4694 5436 5521
Size: 323 271 407 496 156 95 742 85
Cuts at: 5521 6592 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 496 451 407 399 323
271 250 164 156 109 95 85 81
77 24
BspEI T'CCGG_A
Cuts at: 3768 4267 7038 3768
Size: 499 2771 4105
Fragments arranged by size:
4105 2771 499
BspHI T'CATG_A
Cuts at: 3244 3819 4179 5264 6994 3244
Size: 575 360 1085 1730 3625
Fragments arranged by size:
3625 1730 1085 575 360
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2117 2858 3843 4730 844
Size: 1273 741 985 887 3489
Fragments arranged by size:
3489 1273 985 887 741
BsrBI CCG'CTC
Cuts at: 1394 1823 2555 5262 1394
Size: 429 732 2707 3507
Fragments arranged by size:
3507 2707 732 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3672 3918 5870 6044 65
Size: 1183 2424 246 1952 174 1396
Fragments arranged by size:
2424 1952 1396 1183 246 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2179 2339 2343 6019 6206 1700
Size: 110 369 160 4 3676 187 2869
Fragments arranged by size:
3676 2869 369 187 160 110 4
BsrGI T'GTAC_A
Cuts at: 96 2271 2777 96
Size: 2175 506 4694
Fragments arranged by size:
4694 2175 506
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7375
BssSI C'ACGA_G
Cuts at: 5435 6447 5435
Size: 1012 6363
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2678 794
Size: 1884 5491
BstBI TT'CG_AA
Cuts at: 3358 6179 3358
Size: 2821 4554
BstEII G'GTnAC_C
Cuts at: 1184 2587 4162 1184
Size: 1403 1575 4397
Fragments arranged by size:
4397 1575 1403
BstXI CCAn_nnnn'nTGG
Cuts at: 2808 2914 3802 2808
Size: 106 888 6381
Fragments arranged by size:
6381 888 106
BstYI r'GATC_y
Cuts at: 1972 2382 2443 3157 4041 4705 5072 5457 5474
Size: 410 61 714 884 664 367 385 17
Cuts at: 5474 6926 7012 7024 7370 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 884 714 664 410 385 367
346 86 61 17 12
BstZ17I GTA'TAC
Cuts at: 4131 4131
Size: 7375
Bsu36I CC'TnA_GG
Cuts at: 2891 3701 2891
Size: 810 6565
BtgI C'CryG_G
Cuts at: 513 1065 2004 2066 3801 513
Size: 552 939 62 1735 4087
Fragments arranged by size:
4087 1735 939 552 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3522 487
Size: 683 56 2296 4340
Fragments arranged by size:
4340 2296 683 56
BtsI GCAGTG_nn'
Cuts at: 2203 2457 2672 4443 4513 4527 4891 5685 5705
Size: 254 215 1771 70 14 364 794 20
Cuts at: 5705 2203
Size: 3873
Fragments arranged by size:
3873 1771 794 364 254 215 70 20
14
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7375
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7340
DraI TTT'AAA
Cuts at: 2574 4679 2574
Size: 2105 5270
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3219 6382 809
Size: 507 1903 3163 1802
Fragments arranged by size:
3163 1903 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2030 2341 2755
Size: 54 1425 50 275 18 200 311 414
Cuts at: 2755 3744 4116 4822 5711 7340 8
Size: 989 372 706 889 1629 43
Fragments arranged by size:
1629 1425 989 889 706 414 372 311
275 200 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2030 2341 4822 1537
Size: 275 218 311 2481 4090
Fragments arranged by size:
4090 2481 311 275 218
EarI CTCTTCn'nnn_
Cuts at: 1353 2647 3244 3687 5305 1353
Size: 1294 597 443 1618 3423
Fragments arranged by size:
3423 1618 1294 597 443
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2032 2107 5962 6336 6482 387
Size: 1064 138 443 75 3855 374 146 1280
Fragments arranged by size:
3855 1280 1064 443 374 146 138 75
EcoICRI GAG'CTC
Cuts at: 2762 3945 2762
Size: 1183 6192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2336 3238 3548 3635 3875 4127
Size: 393 521 183 902 310 87 240 252
Cuts at: 4127 4330 4382 4481 5438 6035 6822 7090 1239
Size: 203 52 99 957 597 787 268 1524
Fragments arranged by size:
1524 957 902 787 597 521 393 310
268 252 240 203 183 99 87 52
EcoNI CCTnn'n_nnAGG
Cuts at: 3057 3057
Size: 7375
EcoO109I rG'GnC_Cy
Cuts at: 2495 2549 2797 2863 2918 2919 3049 3062 3071
Size: 54 248 66 55 1 130 13 9
Cuts at: 3071 3169 3265 3266 3832 4405 2495
Size: 98 96 1 566 573 5465
Fragments arranged by size:
5465 573 566 248 130 98 96 66
55 54 13 9 1 1
EcoRI G'AATT_C
Cuts at: 4684 5062 4684
Size: 378 6997
FspI TGC'GCA
Cuts at: 5881 5881
Size: 7375
HaeII r_GCGC'y
Cuts at: 1678 2012 2332 2979 4333 6522 1678
Size: 334 320 647 1354 2189 2531
Fragments arranged by size:
2531 2189 1354 647 334 320
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2152 2184 2944 2976 6016
Size: 32 135 32 580 32 760 32 3040
Cuts at: 6016 6048 6090 6122 1373
Size: 32 42 32 2626
Fragments arranged by size:
3040 2626 760 580 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4719 4965 669
Size: 988 281 2781 246 3079
Fragments arranged by size:
3079 2781 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2708 748
Size: 1960 5415
HpaI GTT'AAC
Cuts at: 4965 4965
Size: 7375
KpnI G_GTAC'C
Cuts at: 3296 4700 3296
Size: 1404 5971
MfeI C'AATT_G
Cuts at: 4974 4974
Size: 7375
MluI A'CGCG_T
Cuts at: 7251 7251
Size: 7375
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2497 6489 6673 1305
Size: 1192 3992 184 2007
Fragments arranged by size:
3992 2007 1192 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3746 10
Size: 54 1425 343 1914 3639
Fragments arranged by size:
3639 1914 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2699 3249 3800 4007 5333 5692 5851
Size: 648 1533 550 551 207 1326 359 159
Cuts at: 5851 518
Size: 2042
Fragments arranged by size:
2042 1533 1326 648 551 550 359 207
159
MspA1I CmG'CkG
Cuts at: 1606 2047 2053 2068 3613 4381 4791 5468 6616
Size: 441 6 15 1545 768 410 677 1148
Cuts at: 6616 6861 1606
Size: 245 2120
Fragments arranged by size:
2120 1545 1148 768 677 441 410 245
15 6
NaeI GCC'GGC
Cuts at: 2341 2341
Size: 7375
NcoI C'CATG_G
Cuts at: 513 1065 2004 3801 513
Size: 552 939 1797 4087
Fragments arranged by size:
4087 1797 939 552
NdeI CA'TA_TG
Cuts at: 387 4201 387
Size: 3814 3561
NgoMIV G'CCGG_C
Cuts at: 2339 2339
Size: 7375
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7375
NotI GC'GGCC_GC
Cuts at: 4822 4822
Size: 7375
NspI r_CATG'y
Cuts at: 1132 1229 3842 3991 4733 6278 1132
Size: 97 2613 149 742 1545 2229
Fragments arranged by size:
2613 2229 1545 742 149 97
PasI CC'CwG_GG
Cuts at: 1348 3067 3562 1348
Size: 1719 495 5161
Fragments arranged by size:
5161 1719 495
PciI A'CATG_T
Cuts at: 6274 6274
Size: 7375
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2260 2520 4205 1108
Size: 1152 260 1685 4278
Fragments arranged by size:
4278 1685 1152 260
PfoI T'CCnGG_A
Cuts at: 2109 2859 3857 4034 2109
Size: 750 998 177 5450
Fragments arranged by size:
5450 998 750 177
PmeI GTTT'AAAC
Cuts at: 4679 4679
Size: 7375
PmlI CAC'GTG
Cuts at: 3742 4187 3742
Size: 445 6930
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3539 3571 5444 5476 6957 6989 3539
Size: 32 1873 32 1481 32 3925
Fragments arranged by size:
3925 1873 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2495 2549 2863 3071 3169 3832 4405 2495
Size: 54 314 208 98 663 573 5465
Fragments arranged by size:
5465 663 573 314 208 98 54
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7375
PsiI TTA'TAA
Cuts at: 4945 4945
Size: 7375
PspOMI G'GGCC_C
Cuts at: 2918 3265 2918
Size: 347 7028
PstI C_TGCA'G
Cuts at: 830 2198 2683 4647 4727 830
Size: 1368 485 1964 80 3478
Fragments arranged by size:
3478 1964 1368 485 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7375
PvuII CAG'CTG
Cuts at: 3613 4381 3613
Size: 768 6607
SacI G_AGCT'C
Cuts at: 2764 3947 2764
Size: 1183 6192
SacII CC_GC'GG
Cuts at: 2069 2069
Size: 7375
SalI G'TCGA_C
Cuts at: 1936 4717 1936
Size: 2781 4594
SanDI GG'GwC_CC
Cuts at: 2495 2549 3071 2495
Size: 54 522 6799
Fragments arranged by size:
6799 522 54
SbfI CC_TGCA'GG
Cuts at: 4727 4727
Size: 7375
ScaI AGT'ACT
Cuts at: 5623 7335 5623
Size: 1712 5663
SexAI A'CCwGG_T
Cuts at: 2865 2865
Size: 7375
SfcI C'TryA_G
Cuts at: 826 1045 2194 2679 2768 2884 4045 4486 4643
Size: 219 1149 485 89 116 1161 441 157
Cuts at: 4643 4723 5858 6539 6730 826
Size: 80 1135 681 191 1471
Fragments arranged by size:
1471 1161 1149 1135 681 485 441 219
191 157 116 89 80
SfiI GGCCn_nnn'nGGCC
Cuts at: 3264 3264
Size: 7375
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7375
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7375
SmaI CCC'GGG
Cuts at: 1872 3413 4702 1872
Size: 1541 1289 4545
Fragments arranged by size:
4545 1541 1289
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3679 5479 6380 6642 820
Size: 197 926 1736 1800 901 262 1553
Fragments arranged by size:
1800 1736 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5166 493
Size: 4673 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7375
SphI G_CATG'C
Cuts at: 1132 3842 3991 4733 1132
Size: 2710 149 742 3774
Fragments arranged by size:
3774 2710 742 149
SspI AAT'ATT
Cuts at: 5 52 5299 5
Size: 47 5247 2081
Fragments arranged by size:
5247 2081 47
StuI AGG'CCT
Cuts at: 1517 2634 3015 1517
Size: 1117 381 5877
Fragments arranged by size:
5877 1117 381
StyI C'CwwG_G
Cuts at: 513 1065 2004 2913 2955 3304 3801 4814 513
Size: 552 939 909 42 349 497 1013 3074
Fragments arranged by size:
3074 1013 939 909 552 497 349 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2221 2473 2929 4514 5402 5419 5572
Size: 1255 641 252 456 1585 888 17 153
Cuts at: 5572 5757 6202 325
Size: 185 445 1498
Fragments arranged by size:
1585 1498 1255 888 641 456 445 252
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2271 2777 5621
Size: 274 80 33 51 1443 294 506 2844
Cuts at: 5621 7333 96
Size: 1712 138
Fragments arranged by size:
2844 1712 1443 506 294 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2136 2168 2731 2763 7335 7367 2136
Size: 32 563 32 4572 32 2144
Fragments arranged by size:
4572 2144 563 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2164 3401 1316
Size: 848 1237 5290
Fragments arranged by size:
5290 1237 848
XbaI T'CTAG_A
Cuts at: 4711 4711
Size: 7375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2363 2363
Size: 7375
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7375
XmaI C'CCGG_G
Cuts at: 1870 3411 4700 1870
Size: 1541 1289 4545
Fragments arranged by size:
4545 1541 1289
XmnI GAAnn'nnTTC
Cuts at: 5504 5504
Size: 7375
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5183 276
Size: 53 83 186 936 3649 2468
Fragments arranged by size:
3649 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI DraIII
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PspXI PsrI RsrII SapI SfoI SrfI SwaI