Restriction map for
pFN21AB4431
(Linear) (Six-base) MAPSORT of: KIBB4431.seq Check: 4233 from: 1 to: 2670
KIBB4431.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AccI GT'mk_AC
Cuts at: 0 2125 2670
Size: 2125 545
Acc65I G'GTAC_C
Cuts at: 0 1287 2670
Size: 1287 1383
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 331 1630 2670
Size: 148 183 1299 1040
Fragments arranged by size:
1299 1040 183 148
AfeI AGC'GCT
Cuts at: 0 325 972 2670
Size: 325 647 1698
Fragments arranged by size:
1698 647 325
AflIII A'CryG_T
Cuts at: 0 1176 1734 2141 2179 2279 2670
Size: 1176 558 407 38 100 391
Fragments arranged by size:
1176 558 407 391 100 38
AhdI GACnn_n'nnGTC
Cuts at: 0 953 2670
Size: 953 1717
AlwNI CAG_nnn'CTG
Cuts at: 0 652 745 1224 1893 2537 2666 2670
Size: 652 93 479 669 644 129 4
Fragments arranged by size:
669 652 644 479 129 93 4
ApaI G_GGCC'C
Cuts at: 0 917 1264 2670
Size: 917 347 1406
Fragments arranged by size:
1406 917 347
AvaI C'yCGr_G
Cuts at: 0 67 1039 1406 1462 2328 2670
Size: 67 972 367 56 866 342
Fragments arranged by size:
972 866 367 342 67 56
BamHI G'GATC_C
Cuts at: 0 1152 2670
Size: 1152 1518
BanI G'GyrC_C
Cuts at: 0 347 864 936 1287 2670
Size: 347 517 72 351 1383
Fragments arranged by size:
1383 517 351 347 72
BanII G_rGCy'C
Cuts at: 0 35 144 759 917 1264 1942 2438 2670
Size: 35 109 615 158 347 678 496 232
Fragments arranged by size:
678 615 496 347 232 158 109 35
BbvCI CC'TCA_GC
Cuts at: 0 7 304 2429 2670
Size: 7 297 2125 241
Fragments arranged by size:
2125 297 241 7
BglI GCCn_nnn'nGGC
Cuts at: 0 63 393 1259 2670
Size: 63 330 866 1411
Fragments arranged by size:
1411 866 330 63
BglII A'GATC_T
Cuts at: 0 377 438 2036 2670
Size: 377 61 1598 634
Fragments arranged by size:
1598 634 377 61
BlpI GC'TnA_GC
Cuts at: 0 2570 2670
Size: 2570 100
Bme1580I G_kGCm'C
Cuts at: 0 836 917 941 1264 2670
Size: 836 81 24 323 1406
Fragments arranged by size:
1406 836 323 81 24
BmgBI CAC'GTC
Cuts at: 0 2142 2280 2670
Size: 2142 138 390
Fragments arranged by size:
2142 390 138
BmrI ACTGGGnnnn_n'
Cuts at: 0 268 942 2525 2670
Size: 268 674 1583 145
Fragments arranged by size:
1583 674 268 145
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1641 2670
Size: 1641 1029
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1233 1543 1870 2122 2325 2377 2476 2670
Size: 1233 310 327 252 203 52 99 194
Fragments arranged by size:
1233 327 310 252 203 194 99 52
Bpu10I CC'TnA_GC
Cuts at: 0 7 304 1936 2429 2670
Size: 7 297 1632 493 241
Fragments arranged by size:
1632 493 297 241 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1659 2670
Size: 1659 1011
BsaI GGTCTCn'nnnn_
Cuts at: 0 1236 1955 2670
Size: 1236 719 715
Fragments arranged by size:
1236 719 715
BsaAI yAC'GTr
Cuts at: 0 1177 1737 2182 2670
Size: 1177 560 445 488
Fragments arranged by size:
1177 560 488 445
BsaHI Gr'CG_yC
Cuts at: 0 332 2191 2670
Size: 332 1859 479
Fragments arranged by size:
1859 479 332
BsaWI w'CCGG_w
Cuts at: 0 1763 1898 2262 2670
Size: 1763 135 364 408
Fragments arranged by size:
1763 408 364 135
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 131 161 2266 2296 2670
Size: 131 30 2105 30 374
Fragments arranged by size:
2105 374 131 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 223 1256 2446 2670
Size: 223 1033 1190 224
Fragments arranged by size:
1190 1033 224 223
BseYI C'CCAG_C
Cuts at: 0 383 803 963 1148 1604 2491 2670
Size: 383 420 160 185 456 887 179
Fragments arranged by size:
887 456 420 383 185 179 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1268 2152 2670
Size: 1268 884 518
Fragments arranged by size:
1268 884 518
BsiEI CG_ry'CG
Cuts at: 0 28 339 2670
Size: 28 311 2331
Fragments arranged by size:
2331 311 28
BsiHKAI G_wGCw'C
Cuts at: 0 595 759 1535 1942 2594 2670
Size: 595 164 776 407 652 76
Fragments arranged by size:
776 652 595 407 164 76
BsiWI C'GTAC_G
Cuts at: 0 1173 2670
Size: 1173 1497
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1191 1353 1363 1789 2670
Size: 1191 162 10 426 881
Fragments arranged by size:
1191 881 426 162 10
Bsp1286I G_dGCh'C
Cuts at: 0 35 144 595 759 836 917 941 1264
Size: 35 109 451 164 77 81 24 323
Cuts at: 1264 1535 1942 2438 2594 2670
Size: 271 407 496 156 76
Fragments arranged by size:
496 451 407 323 271 164 156 109
81 77 76 35 24
BspEI T'CCGG_A
Cuts at: 0 1763 2262 2670
Size: 1763 499 408
Fragments arranged by size:
1763 499 408
BspHI T'CATG_A
Cuts at: 0 1239 1814 2174 2670
Size: 1239 575 360 496
Fragments arranged by size:
1239 575 496 360
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 112 853 1838 2670
Size: 112 741 985 832
Fragments arranged by size:
985 832 741 112
BsrBI CCG'CTC
Cuts at: 0 550 2670
Size: 550 2120
BsrDI GCAATG_nn'
Cuts at: 0 1667 1913 2670
Size: 1667 246 757
Fragments arranged by size:
1667 757 246
BsrFI r'CCGG_y
Cuts at: 0 174 334 338 2670
Size: 174 160 4 2332
Fragments arranged by size:
2332 174 160 4
BsrGI T'GTAC_A
Cuts at: 0 266 772 2670
Size: 266 506 1898
Fragments arranged by size:
1898 506 266
BstAPI GCAn_nnn'nTGC
Cuts at: 0 673 2670
Size: 673 1997
BstBI TT'CG_AA
Cuts at: 0 1353 2670
Size: 1353 1317
BstEII G'GTnAC_C
Cuts at: 0 582 2157 2670
Size: 582 1575 513
Fragments arranged by size:
1575 582 513
BstXI CCAn_nnnn'nTGG
Cuts at: 0 803 909 1797 2670
Size: 803 106 888 873
Fragments arranged by size:
888 873 803 106
BstYI r'GATC_y
Cuts at: 0 377 438 1152 2036 2670
Size: 377 61 714 884 634
Fragments arranged by size:
884 714 634 377 61
BstZ17I GTA'TAC
Cuts at: 0 2126 2670
Size: 2126 544
Bsu36I CC'TnA_GG
Cuts at: 0 886 1696 2670
Size: 886 810 974
Fragments arranged by size:
974 886 810
BtgI C'CryG_G
Cuts at: 0 61 1796 2670
Size: 61 1735 874
Fragments arranged by size:
1735 874 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1517 2670
Size: 1517 1153
BtsI GCAGTG_nn'
Cuts at: 0 198 452 667 2438 2508 2522 2670
Size: 198 254 215 1771 70 14 148
Fragments arranged by size:
1771 254 215 198 148 70 14
DraI TTT'AAA
Cuts at: 0 569 2670
Size: 569 2101
DrdI GACnn_nn'nnGTC
Cuts at: 0 1214 2670
Size: 1214 1456
EaeI y'GGCC_r
Cuts at: 0 25 336 750 1739 2111 2670
Size: 25 311 414 989 372 559
Fragments arranged by size:
989 559 414 372 311 25
EagI C'GGCC_G
Cuts at: 0 25 336 2670
Size: 25 311 2334
Fragments arranged by size:
2334 311 25
EarI CTCTTCn'nnn_
Cuts at: 0 642 1239 1682 2670
Size: 642 597 443 988
Fragments arranged by size:
988 642 597 443
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 27 102 2670
Size: 27 75 2568
Fragments arranged by size:
2568 75 27
EcoICRI GAG'CTC
Cuts at: 0 757 1940 2670
Size: 757 1183 730
Fragments arranged by size:
1183 757 730
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 331 1233 1543 1630 1870 2122 2325
Size: 148 183 902 310 87 240 252 203
Cuts at: 2325 2377 2476 2670
Size: 52 99 194
Fragments arranged by size:
902 310 252 240 203 194 183 148
99 87 52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1052 2670
Size: 1052 1618
EcoO109I rG'GnC_Cy
Cuts at: 0 490 544 792 858 913 914 1044 1057
Size: 490 54 248 66 55 1 130 13
Cuts at: 1057 1066 1164 1260 1261 1827 2400 2670
Size: 9 98 96 1 566 573 270
Fragments arranged by size:
573 566 490 270 248 130 98 96
66 55 54 13 9 1 1
HaeII r_GCGC'y
Cuts at: 0 7 327 974 2328 2670
Size: 7 320 647 1354 342
Fragments arranged by size:
1354 647 342 320 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 147 179 939 971 2670
Size: 147 32 760 32 1699
Fragments arranged by size:
1699 760 147 32 32
HindIII A'AGCT_T
Cuts at: 0 703 2670
Size: 703 1967
KpnI G_GTAC'C
Cuts at: 0 1291 2670
Size: 1291 1379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 2670
Size: 492 2178
MscI TGG'CCA
Cuts at: 0 1741 2670
Size: 1741 929
MslI CAynn'nnrTG
Cuts at: 0 694 1244 1795 2002 2670
Size: 694 550 551 207 668
Fragments arranged by size:
694 668 551 550 207
MspA1I CmG'CkG
Cuts at: 0 42 48 63 1608 2376 2670
Size: 42 6 15 1545 768 294
Fragments arranged by size:
1545 768 294 42 15 6
NaeI GCC'GGC
Cuts at: 0 336 2670
Size: 336 2334
NcoI C'CATG_G
Cuts at: 0 1796 2670
Size: 1796 874
NdeI CA'TA_TG
Cuts at: 0 2196 2670
Size: 2196 474
NgoMIV G'CCGG_C
Cuts at: 0 334 2670
Size: 334 2336
NspI r_CATG'y
Cuts at: 0 1837 1986 2670
Size: 1837 149 684
Fragments arranged by size:
1837 684 149
PasI CC'CwG_GG
Cuts at: 0 1062 1557 2670
Size: 1062 495 1113
Fragments arranged by size:
1113 1062 495
PflMI CCAn_nnn'nTGG
Cuts at: 0 255 515 2200 2670
Size: 255 260 1685 470
Fragments arranged by size:
1685 470 260 255
PfoI T'CCnGG_A
Cuts at: 0 104 854 1852 2029 2670
Size: 104 750 998 177 641
Fragments arranged by size:
998 750 641 177 104
PmlI CAC'GTG
Cuts at: 0 1737 2182 2670
Size: 1737 445 488
Fragments arranged by size:
1737 488 445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1534 1566 2670
Size: 1534 32 1104
Fragments arranged by size:
1534 1104 32
PpuMI rG'GwC_Cy
Cuts at: 0 490 544 858 1066 1164 1827 2400 2670
Size: 490 54 314 208 98 663 573 270
Fragments arranged by size:
663 573 490 314 270 208 98 54
PspOMI G'GGCC_C
Cuts at: 0 913 1260 2670
Size: 913 347 1410
Fragments arranged by size:
1410 913 347
PstI C_TGCA'G
Cuts at: 0 193 678 2642 2670
Size: 193 485 1964 28
Fragments arranged by size:
1964 485 193 28
PvuII CAG'CTG
Cuts at: 0 1608 2376 2670
Size: 1608 768 294
Fragments arranged by size:
1608 768 294
SacI G_AGCT'C
Cuts at: 0 759 1942 2670
Size: 759 1183 728
Fragments arranged by size:
1183 759 728
SacII CC_GC'GG
Cuts at: 0 64 2670
Size: 64 2606
SanDI GG'GwC_CC
Cuts at: 0 490 544 1066 2670
Size: 490 54 522 1604
Fragments arranged by size:
1604 522 490 54
SexAI A'CCwGG_T
Cuts at: 0 860 2670
Size: 860 1810
SfcI C'TryA_G
Cuts at: 0 189 674 763 879 2040 2481 2638 2670
Size: 189 485 89 116 1161 441 157 32
Fragments arranged by size:
1161 485 441 189 157 116 89 32
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1259 2670
Size: 1259 1411
SmaI CCC'GGG
Cuts at: 0 1408 2670
Size: 1408 1262
SmlI C'TyrA_G
Cuts at: 0 1674 2670
Size: 1674 996
SphI G_CATG'C
Cuts at: 0 1837 1986 2670
Size: 1837 149 684
Fragments arranged by size:
1837 684 149
StuI AGG'CCT
Cuts at: 0 629 1010 2670
Size: 629 381 1660
Fragments arranged by size:
1660 629 381
StyI C'CwwG_G
Cuts at: 0 908 950 1299 1796 2670
Size: 908 42 349 497 874
Fragments arranged by size:
908 874 497 349 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 216 468 924 2509 2670
Size: 216 252 456 1585 161
Fragments arranged by size:
1585 456 252 216 161
TatI w'GTAC_w
Cuts at: 0 266 772 2670
Size: 266 506 1898
Fragments arranged by size:
1898 506 266
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 131 163 726 758 2670
Size: 131 32 563 32 1912
Fragments arranged by size:
1912 563 131 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 159 1396 2670
Size: 159 1237 1274
Fragments arranged by size:
1274 1237 159
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 358 2670
Size: 358 2312
XmaI C'CCGG_G
Cuts at: 0 1406 2670
Size: 1406 1264
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AhdI AlwNI ApaI
AvaI BamHI BanI BanII BbvCI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I HindIII KpnI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NspI PasI PflMI PfoI PmlI
PpiI PpuMI PspOMI PstI PvuII SacI SacII SanDI
SexAI SfcI SfiI SmaI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AleI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BbsI BcgI BciVI BclI BfrBI BmtI BsaBI BsmI
BssHII BssSI ClaI CspCI DraIII EcoRI EcoRV FalI
FseI FspI FspAI HincII HpaI KasI MfeI MluI
NarI NheI NotI NruI NsiI PacI PciI PmeI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI
SbfI ScaI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI XbaI XhoI XmnI ZraI