Restriction map for
pFN21AB4391
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccI AccII AclI
AflII AgeI AhdI AocI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BsaBI BsaI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspMI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DraII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HindIII KasI KpnI
KspI MamI MfeI MflI MluI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tth111I TthHB8I Van91I
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 39 TCCGGA T^CCGGA
AcyI 778 GACGCC G[GA]^CG[CT]C
AflIII 499 ACATGT A^C[GA][CT]GT
Alw44I 1381 GTGCAC G^TGCAC
AlwNI 24 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1052 AGCGCT AGC^GCT
ApaLI 1381 GTGCAC G^TGCAC
BalI 162 TGGCCA TGG^CCA
BanII 18 GAGCTC G[GA]GC[CT]^C
BbsI 1100 GTCTTC GAAGAC(2/6)
BlpI 854 GCTCAGC GC^T[ACGT]AGC
BpmI 21 CTCCAG CTGGAG(16/14)
Bpu1102I 854 GCTCAGC GC^T[ACGT]AGC
BpuAI 1100 GTCTTC GAAGAC(2/6)
BsaHI 778 GACGCC G[GA]^CG[CT]C
BsaWI 39 TCCGGA [AT]^CCGG[AT]
BseAI 39 TCCGGA T^CCGGA
BseRI 1483 GAGGAG GAGGAG(10/8)
BspEI 39 TCCGGA T^CCGGA
BspHI 506 TCATGA T^CATGA
BspLU11I 499 ACATGT A^CATGT
BsrBI 852 CCGCTC CCGCTC(-3/-3)
BsrDI 33 CATTGC GCAATG(2/0)
BsrFI 95 GCCGGT [GA]^CCGG[CT]
BstAPI 86 GCAGCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 570 GCCCGC GC[ACGT]^[ACGT]GC
CelII 854 GCTCAGC GC^T[ACGT]AGC
Cfr10I 95 GCCGGT [GA]^CCGG[CT]
DraIII 674 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 162 TGGCCA [CT]^GGCC[GA]
EarI 658 GAAGAG CTCTTC(1/4)
Eco47III 1052 AGCGCT AGC^GCT
HaeII 1052 AGCGCT [GA]GCGC^[CT]
Hin1I 778 GACGCC G[GA]^CG[CT]C
HincII 964 GTTAAC GT[CT]^[GA]AC
HindII 964 GTTAAC GT[CT]^[GA]AC
HpaI 964 GTTAAC GTT^AAC
HphI 1494 GGTGA GGTGA(8/7)
Ksp632I 658 GAAGAG CTCTTC(1/4)
MluNI 162 TGGCCA TGG^CCA
MroI 39 TCCGGA T^CCGGA
MscI 162 TGGCCA TGG^CCA
MslI 1450 CACTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1386 CCATGG C^CATGG
NdeI 975 CATATG CA^TATG
NspI 499 ACATGT [GA]CATG^[CT]
PvuII 380 CAGCTG CAG^CTG
RcaI 506 TCATGA T^CATGA
SacI 18 GAGCTC GAGCT^C
ScaI 1462 AGTACT AGT^ACT
SnaBI 1279 TACGTA TAC^GTA
XbaI 991 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwI 1003 GATCC GGATC(4/5)
1546 GGATC
AspHI 18 GAGCTC G[AT]GC[AT]^C
1381 GTGCAC
BcnI 1344 CCCGG CC^[GC]GG
1394 CCGGG
BmyI 18 GAGCTC G[AGT]GC[ACT]^C
1381 GTGCAC
BsaAI 1279 TACGTA [CT]AC^GT[GA]
1522 TACGTG
BsiHKAI 18 GAGCTC G[AT]GC[AT]^C
1381 GTGCAC
BsmAI 1135 GAGAC GTCTC(1/5)
1486 GAGAC
BsmFI 324 GGGAC GGGAC(10/14)
982 GGGAC
BsmI 510 GAATGC GAATGC(1/-1)
947 GCATTC
Bsp1286I 18 GAGCTC G[AGT]GC[ACT]^C
1381 GTGCAC
BsrI 327 ACTGG ACTGG(1/-1)
1078 CCAGT
BstF5I 516 CATCC GGATG(2/0)
1512 CATCC
CfoI 795 GCGC GCG^C
1053 GCGC
DpnII 1003 GATC ^GATC
1547 GATC
DsaI 575 CCGTGG C^C[GA][CT]GG
1386 CCATGG
EcoRI 787 GAATTC G^AATTC
873 GAATTC
FauI 571 CCCGC CCCGC(4/6)
851 CCCGC
FokI 516 CATCC GGATG(9/13)
1512 CATCC
HgaI 778 GACGC GACGC(5/10)
26 GCGTC
HhaI 795 GCGC GCG^C
1053 GCGC
HinP1I 795 GCGC G^CGC
1053 GCGC
MaeII 1280 ACGT A^CGT
1523 ACGT
MboI 1003 GATC ^GATC
1547 GATC
NciI 1344 CCCGG CC^[GC]GG
1394 CCGGG
NdeII 1003 GATC ^GATC
1547 GATC
PleI 43 GACTC GAGTC(4/5)
718 GAGTC
PstI 84 CTGCAG CTGCA^G
865 CTGCAG
Sau3AI 1003 GATC ^GATC
1547 GATC
SfaNI 187 GATGC GCATC(5/9)
916 GCATC
Tsp45I 525 GTCAC ^GT[GC]AC
928 GTCAC
XcmI 317 CCAAGCTGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1533 CCAGGCCAAAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BfaI BsaJI BsiYI
BslI BssKI BstNI Cfr13I DdeI
EcoRII EcoT14I Fnu4HI HaeIII HapII
HinfI HpaII ItaI MaeI MaeIII
MboII MnlI MseI MspA1I MspI
MvaI MwoI NlaIII NlaIV RsaI
Sau96I ScrFI SfcI StuI StyI
TfiI Tru9I TseI Tsp509I TspRI