(Circular) (Six-base) MAPSORT of: pFN21AB4391.seq Check: 5079 from: 1 to: 6265 pFN21AB4391 6265 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4075 278 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3608 1937 Size: 1671 4594 Acc65I G'GTAC_C Cuts at: 3586 3586 Size: 6265 AclI AA'CG_TT Cuts at: 1563 4392 4765 1563 Size: 2829 373 3063 Fragments arranged by size: 3063 2829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2510 2816 4328 5712 5980 1632 Size: 878 306 1512 1384 268 1917 Fragments arranged by size: 1917 1512 1384 878 306 268 AfeI AGC'GCT Cuts at: 3059 3059 Size: 6265 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6068 AflIII A'CryG_T Cuts at: 1203 2504 5164 6141 1203 Size: 1301 2660 977 1327 Fragments arranged by size: 2660 1327 1301 977 AgeI A'CCGG_T Cuts at: 5096 5096 Size: 6265 AhdI GACnn_n'nnGTC Cuts at: 4994 4994 Size: 6265 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6265 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6265 AlwNI CAG_nnn'CTG Cuts at: 2034 4008 5580 2034 Size: 1974 1572 2719 Fragments arranged by size: 2719 1974 1572 ApaLI G'TGCA_C Cuts at: 3386 4322 5478 3386 Size: 936 1156 4173 Fragments arranged by size: 4173 1156 936 ApoI r'AATT_y Cuts at: 1493 2491 2792 2878 3290 3574 3800 3952 6061 Size: 998 301 86 412 284 226 152 2109 Cuts at: 6061 1493 Size: 1697 Fragments arranged by size: 2109 1697 998 412 301 284 226 152 86 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 3590 1870 Size: 73 1647 4545 Fragments arranged by size: 4545 1647 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3270 3303 3270 Size: 33 6232 BamHI G'GATC_C Cuts at: 3595 3962 3595 Size: 367 5898 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3586 5041 618 Size: 325 217 627 1799 1455 1842 Fragments arranged by size: 1842 1799 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2027 3584 1391 Size: 636 1557 4072 Fragments arranged by size: 4072 1557 636 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3098 928 Size: 971 1199 4095 Fragments arranged by size: 4095 1199 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4456 4490 1903 Size: 34 2519 34 3678 Fragments arranged by size: 3678 2519 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4157 5367 4157 Size: 1210 5055 BclI T'GATC_A Cuts at: 1548 1548 Size: 6265 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4876 136 Size: 107 122 71 631 605 3204 1525 Fragments arranged by size: 3204 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6260 6260 Size: 6265 BlpI GC'TnA_GC Cuts at: 2860 3682 2860 Size: 822 5443 Bme1580I G_kGCm'C Cuts at: 1790 3390 4326 5482 1790 Size: 1600 936 1156 2573 Fragments arranged by size: 2573 1600 1156 936 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6265 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3996 4954 442 Size: 416 53 83 399 24 2579 958 1753 Fragments arranged by size: 2579 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6265 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2972 2972 Size: 6265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2011 4925 1239 Size: 772 2914 2579 Fragments arranged by size: 2914 2579 772 Bpu10I CC'TnA_GC Cuts at: 2021 2021 Size: 6265 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4390 5255 5553 4390 Size: 865 298 5102 Fragments arranged by size: 5102 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4928 882 Size: 617 3429 2219 Fragments arranged by size: 3429 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3286 3529 4056 493 Size: 711 2082 243 527 2702 Fragments arranged by size: 2702 2082 711 527 243 BsaBI GATnn'nnATC Cuts at: 6259 6259 Size: 6265 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2784 4072 4454 5089 Size: 53 83 186 936 1251 1288 382 635 Cuts at: 5089 275 Size: 1451 Fragments arranged by size: 1451 1288 1251 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2044 4698 5096 5370 5517 5928 2044 Size: 2654 398 274 147 411 2381 Fragments arranged by size: 2654 2381 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2009 2039 1178 Size: 30 165 30 606 30 5404 Fragments arranged by size: 5404 606 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3503 1186 Size: 2317 3948 BseYI C'CCAG_C Cuts at: 2326 5468 5964 2326 Size: 3142 496 2627 Fragments arranged by size: 3142 2627 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5692 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3715 4476 5504 1540 Size: 275 186 1714 761 1028 2301 Fragments arranged by size: 2301 1714 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2027 3390 4326 4411 5482 2027 Size: 1363 936 85 1071 2810 Fragments arranged by size: 2810 1363 1071 936 85 BsmI GAATG_Cn' Cuts at: 2521 2952 3776 3869 2521 Size: 431 824 93 4917 Fragments arranged by size: 4917 824 431 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2027 3390 3584 4326 4411 5482 1391 Size: 399 237 1363 194 742 85 1071 2174 Fragments arranged by size: 2174 1363 1071 742 399 237 194 85 BspEI T'CCGG_A Cuts at: 2044 5928 2044 Size: 3884 2381 BspHI T'CATG_A Cuts at: 2511 4154 5884 2511 Size: 1643 1730 2892 Fragments arranged by size: 2892 1730 1643 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3620 844 Size: 2776 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2859 4152 1394 Size: 429 1036 1293 3507 Fragments arranged by size: 3507 1293 1036 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2037 4760 4934 65 Size: 1183 789 2723 174 1396 Fragments arranged by size: 2723 1396 1183 789 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2100 4909 5096 1700 Size: 110 290 2809 187 2869 Fragments arranged by size: 2869 2809 290 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6265 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6265 BssSI C'ACGA_G Cuts at: 4325 5337 4325 Size: 1012 5253 BstAPI GCAn_nnn'nTGC Cuts at: 794 2097 794 Size: 1303 4962 BstBI TT'CG_AA Cuts at: 5069 5069 Size: 6265 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6265 BstYI r'GATC_y Cuts at: 1972 3595 3962 4347 4364 5816 5902 5914 6260 Size: 1623 367 385 17 1452 86 12 346 Cuts at: 6260 1972 Size: 1977 Fragments arranged by size: 1977 1623 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2580 3391 513 Size: 552 939 576 811 3387 Fragments arranged by size: 3387 939 811 576 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5526 Fragments arranged by size: 5526 683 56 BtsI GCAGTG_nn' Cuts at: 2463 3781 4575 4595 2463 Size: 1318 794 20 4133 Fragments arranged by size: 4133 1318 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6265 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6230 DraI TTT'AAA Cuts at: 3569 3569 Size: 6265 DraIII CAC_nnn'GTG Cuts at: 2684 2684 Size: 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5272 809 Size: 507 3956 1802 Fragments arranged by size: 3956 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2167 3712 4601 Size: 54 1425 50 275 18 337 1545 889 Cuts at: 4601 6230 8 Size: 1629 43 Fragments arranged by size: 1629 1545 1425 889 337 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3712 1537 Size: 275 1900 4090 Fragments arranged by size: 4090 1900 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2658 4195 1353 Size: 1305 1537 3423 Fragments arranged by size: 3423 1537 1305 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2629 4852 5226 5372 387 Size: 1064 138 1040 2223 374 146 1280 Fragments arranged by size: 2223 1280 1064 1040 374 146 138 EcoICRI GAG'CTC Cuts at: 2025 2025 Size: 6265 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2011 2510 2816 4328 4925 5712 5980 Size: 393 379 499 306 1512 597 787 268 Cuts at: 5980 1239 Size: 1524 Fragments arranged by size: 1524 1512 787 597 499 393 379 306 268 EcoRI G'AATT_C Cuts at: 2792 2878 3574 3952 2792 Size: 86 696 378 5105 Fragments arranged by size: 5105 696 378 86 FspI TGC'GCA Cuts at: 4771 4771 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 3061 5412 1678 Size: 1383 2351 2531 Fragments arranged by size: 2531 2351 1383 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4906 4938 4980 5012 1373 Size: 32 135 32 3334 32 42 32 2626 Fragments arranged by size: 3334 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2971 3609 3855 669 Size: 988 281 1033 638 246 3079 Fragments arranged by size: 3079 1033 988 638 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6265 HpaI GTT'AAC Cuts at: 2971 3855 2971 Size: 884 5381 KpnI G_GTAC'C Cuts at: 3590 3590 Size: 6265 MfeI C'AATT_G Cuts at: 3864 3864 Size: 6265 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2130 5379 5563 1305 Size: 825 3249 184 2007 Fragments arranged by size: 3249 2007 825 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2169 10 Size: 54 1425 343 337 4106 Fragments arranged by size: 4106 1425 343 337 54 MslI CAynn'nnrTG Cuts at: 518 1166 3459 4223 4582 4741 518 Size: 648 2293 764 359 159 2042 Fragments arranged by size: 2293 2042 764 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2387 3377 3447 3681 4358 5506 5751 1606 Size: 781 990 70 234 677 1148 245 2120 Fragments arranged by size: 2120 1148 990 781 677 245 234 70 NcoI C'CATG_G Cuts at: 513 1065 2004 3391 513 Size: 552 939 1387 3387 Fragments arranged by size: 3387 1387 939 552 NdeI CA'TA_TG Cuts at: 387 2981 387 Size: 2594 3671 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6265 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NspI r_CATG'y Cuts at: 1132 1229 2508 3623 5168 1132 Size: 97 1279 1115 1545 2229 Fragments arranged by size: 2229 1545 1279 1115 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6265 PciI A'CATG_T Cuts at: 2504 5164 2504 Size: 2660 3605 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6265 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4334 4366 5847 5879 4334 Size: 32 1481 32 4720 Fragments arranged by size: 4720 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6265 PsiI TTA'TAA Cuts at: 3835 3835 Size: 6265 PstI C_TGCA'G Cuts at: 830 2093 2874 3617 830 Size: 1263 781 743 3478 Fragments arranged by size: 3478 1263 781 743 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 PvuII CAG'CTG Cuts at: 2387 2387 Size: 6265 SacI G_AGCT'C Cuts at: 2027 2027 Size: 6265 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SbfI CC_TGCA'GG Cuts at: 3617 3617 Size: 6265 ScaI AGT'ACT Cuts at: 3469 4513 6225 3469 Size: 1044 1712 3509 Fragments arranged by size: 3509 1712 1044 SfcI C'TryA_G Cuts at: 826 1045 2089 2275 2870 3613 4748 5429 5620 Size: 219 1044 186 595 743 1135 681 191 Cuts at: 5620 826 Size: 1471 Fragments arranged by size: 1471 1135 1044 743 681 595 219 191 186 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6265 SmaI CCC'GGG Cuts at: 1872 3592 1872 Size: 1720 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4369 5270 5532 820 Size: 197 926 2426 901 262 1553 Fragments arranged by size: 2426 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3286 4056 493 Size: 2793 770 2702 Fragments arranged by size: 2793 2702 770 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 3623 1132 Size: 2491 3774 SspI AAT'ATT Cuts at: 5 52 4189 5 Size: 47 4137 2081 Fragments arranged by size: 4137 2081 47 StuI AGG'CCT Cuts at: 1517 2484 3306 3512 1517 Size: 967 822 206 4270 Fragments arranged by size: 4270 967 822 206 StyI C'CwwG_G Cuts at: 513 1065 2004 2117 2635 3216 3391 3704 513 Size: 552 939 113 518 581 175 313 3074 Fragments arranged by size: 3074 939 581 552 518 313 175 113 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4292 4309 4462 4647 5092 325 Size: 1255 2712 17 153 185 445 1498 Fragments arranged by size: 2712 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2279 3467 4511 Size: 274 80 33 51 1443 302 1188 1044 Cuts at: 4511 6223 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1188 1044 302 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2009 2041 2145 2177 3380 3412 6225 6257 2009 Size: 32 104 32 1203 32 2813 32 2017 Fragments arranged by size: 2813 2017 1203 104 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6265 XbaI T'CTAG_A Cuts at: 2996 3601 2996 Size: 605 5660 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2329 3545 2329 Size: 1216 5049 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6265 XmaI C'CCGG_G Cuts at: 1870 3590 1870 Size: 1720 4545 XmnI GAAnn'nnTTC Cuts at: 4394 4394 Size: 6265 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4073 276 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI