Restriction map for
pFN21AB4388
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI AlwI AocI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DpnII DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FbaI FseI FspI HapII
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeII
MboI MfeI MflI MluI MroI
MspA1I MspI MunI MvnI NaeI
NarI NciI NdeII NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI Sau3AI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SphI Sse8387I SspBI
SspI SwaI Tsp45I Tth111I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AfaI 968 GTAC GT^AC
Alw44I 164 GTGCAC G^TGCAC
AlwNI 888 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 743 AGCGCT AGC^GCT
ApaLI 164 GTGCAC G^TGCAC
AspHI 164 GTGCAC G[AT]GC[AT]^C
BalI 834 TGGCCA TGG^CCA
BbsI 478 GTCTTC GAAGAC(2/6)
BcgI 842 GCAACCGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 773 CTAG C^TAG
BmyI 164 GTGCAC G[AGT]GC[ACT]^C
BpmI 429 CTGGAG CTGGAG(16/14)
BpuAI 478 GTCTTC GAAGAC(2/6)
BsaBI 16 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 724 GTGCAG GTGCAG(16/14)
BsiHKAI 164 GTGCAC G[AT]GC[AT]^C
BsmBI 342 CGTCTC CGTCTC(1/5)
BsmFI 250 GTCCC GGGAC(10/14)
Bsp1286I 164 GTGCAC G[AGT]GC[ACT]^C
BsrDI 384 CATTGC GCAATG(2/0)
BstBI 764 TTCGAA TT^CGAA
BstXI 306 CCAGATATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 954 CTCAG C^T[ACGT]AG
DraII 1023 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 834 TGGCCA [CT]^GGCC[GA]
EarI 336 GAAGAG CTCTTC(1/4)
Eco47III 743 AGCGCT AGC^GCT
EcoO109I 1023 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 689 CCCGC CCCGC(4/6)
HgaI 341 GCGTC GACGC(5/10)
Ksp632I 336 GAAGAG CTCTTC(1/4)
MaeI 773 CTAG C^TAG
MamI 16 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 834 TGGCCA TGG^CCA
MscI 834 TGGCCA TGG^CCA
NdeI 215 CATATG CA^TATG
NlaIV 249 GGTCCC GG[ACGT]^[ACGT]CC
NspV 764 TTCGAA TT^CGAA
PflMI 204 CCAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 471 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 968 GTAC GT^AC
SapI 336 GAAGAGC GCTCTTC(1/4)
SfcI 989 CTACAG C^T[GA][CT]AG
SfuI 764 TTCGAA TT^CGAA
SpeI 772 ACTAGT A^CTAGT
StuI 728 AGGCCT AGG^CCT
TfiI 767 GAATC G^A[AT]TC
Van91I 204 CCAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 347 CCATGGCTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AluI 241 AGCT AG^CT
826 AGCT
AvaII 249 GGTCC G^G[AT]CC
756 GGTCC
BseRI 265 CTCCTC GAGGAG(10/8)
289 GAGGAG
CfoI 744 GCGC GCG^C
948 GCGC
DsaI 347 CCATGG C^C[GA][CT]GG
752 CCATGG
EcoRV 515 GATATC GAT^ATC
670 GATATC
HaeII 743 AGCGCT [GA]GCGC^[CT]
947 AGCGCC
HhaI 744 GCGC GCG^C
948 GCGC
HinP1I 744 GCGC G^CGC
948 GCGC
MaeIII 421 GTTAC ^GT[ACGT]AC
884 GTAAC
MseI 403 TTAA T^TAA
1031 TTAA
NcoI 347 CCATGG C^CATGG
752 CCATGG
SfaNI 647 GCATC GCATC(5/9)
853 GCATC
TaqI 469 TCGA T^CGA
765 TCGA
Tru9I 403 TTAA T^TAA
1031 TTAA
Tsp509I 76 AATT ^AATT
906 AATT
TthHB8I 469 TCGA T^CGA
765 TCGA
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI BbvI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MboII MnlI MslI MvaI MwoI
NlaIII PleI Sau96I ScrFI StyI
TseI TspRI