Restriction map for
pFN21AB4388
(Linear) (Six-base) MAPSORT of: KIBB4388.seq Check: 2276 from: 1 to: 1047
KIBB4388.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 678 1047
Size: 355 323 369
Fragments arranged by size:
369 355 323
AfeI AGC'GCT
Cuts at: 0 745 1047
Size: 745 302
AlwNI CAG_nnn'CTG
Cuts at: 0 893 1047
Size: 893 154
ApaLI G'TGCA_C
Cuts at: 0 164 1047
Size: 164 883
BbsI GAAGACnn'nnnn_
Cuts at: 0 471 1047
Size: 471 576
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 831 865 1047
Size: 831 34 182
Fragments arranged by size:
831 182 34
Bme1580I G_kGCm'C
Cuts at: 0 168 1047
Size: 168 879
BmrI ACTGGGnnnn_n'
Cuts at: 0 510 725 1047
Size: 510 215 322
Fragments arranged by size:
510 322 215
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 450 1047
Size: 450 597
BsaBI GATnn'nnATC
Cuts at: 0 20 1047
Size: 20 1027
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 547 577 1047
Size: 547 30 470
Fragments arranged by size:
547 470 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 256 304 1047
Size: 256 48 743
Fragments arranged by size:
743 256 48
BseYI C'CCAG_C
Cuts at: 0 603 1047
Size: 603 444
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 1047
Size: 745 302
BsiHKAI G_wGCw'C
Cuts at: 0 168 1047
Size: 168 879
BsmBI CGTCTCn'nnnn_
Cuts at: 0 348 1047
Size: 348 699
Bsp1286I G_dGCh'C
Cuts at: 0 168 1047
Size: 168 879
BsrDI GCAATG_nn'
Cuts at: 0 383 1047
Size: 383 664
BstBI TT'CG_AA
Cuts at: 0 765 1047
Size: 765 282
BstXI CCAn_nnnn'nTGG
Cuts at: 0 313 1047
Size: 313 734
BtgI C'CryG_G
Cuts at: 0 347 752 1047
Size: 347 405 295
Fragments arranged by size:
405 347 295
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 22 57 855 890 1047
Size: 22 35 798 35 157
Fragments arranged by size:
798 157 35 35 22
EaeI y'GGCC_r
Cuts at: 0 834 1047
Size: 834 213
EarI CTCTTCn'nnn_
Cuts at: 0 331 1047
Size: 331 716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 450 678 1047
Size: 355 95 228 369
Fragments arranged by size:
369 355 228 95
EcoO109I rG'GnC_Cy
Cuts at: 0 1024 1047
Size: 1024 23
EcoRV GAT'ATC
Cuts at: 0 517 672 1047
Size: 517 155 375
Fragments arranged by size:
517 375 155
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1045 1047
Size: 1045 2
HaeII r_GCGC'y
Cuts at: 0 747 951 1047
Size: 747 204 96
Fragments arranged by size:
747 204 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1028 1047
Size: 1028 19
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 800 944 1047
Size: 800 144 103
Fragments arranged by size:
800 144 103
MscI TGG'CCA
Cuts at: 0 836 1047
Size: 836 211
MslI CAynn'nnrTG
Cuts at: 0 142 524 586 1047
Size: 142 382 62 461
Fragments arranged by size:
461 382 142 62
NcoI C'CATG_G
Cuts at: 0 347 752 1047
Size: 347 405 295
Fragments arranged by size:
405 347 295
NdeI CA'TA_TG
Cuts at: 0 216 1047
Size: 216 831
PflMI CCAn_nnn'nTGG
Cuts at: 0 210 1047
Size: 210 837
PshAI GACnn'nnGTC
Cuts at: 0 475 1047
Size: 475 572
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 314 346 1047
Size: 314 32 701
Fragments arranged by size:
701 314 32
SapI GCTCTTCn'nnn_
Cuts at: 0 331 1047
Size: 331 716
SfcI C'TryA_G
Cuts at: 0 989 1047
Size: 989 58
SpeI A'CTAG_T
Cuts at: 0 772 1047
Size: 772 275
StuI AGG'CCT
Cuts at: 0 730 1047
Size: 730 317
StyI C'CwwG_G
Cuts at: 0 11 347 752 1047
Size: 11 336 405 295
Fragments arranged by size:
405 336 295 11
TatI w'GTAC_w
Cuts at: 0 967 1047
Size: 967 80
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 547 579 1047
Size: 547 32 468
Fragments arranged by size:
547 468 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 354 1047
Size: 354 693
Enzymes that do cut:
AcuI AfeI AlwNI ApaLI BbsI BcgI Bme1580I BmrI
BpmI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BsrDI BstBI BstXI BtgI CspCI EaeI EarI
Eco57MI EcoO109I EcoRV FalI HaeII Hin4I MmeI MscI
MslI NcoI NdeI PflMI PshAI PsrI SapI SfcI
SpeI StuI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SphI SrfI SspI SwaI TaqII
Tth111I XbaI XhoI XmaI XmnI ZraI