(Circular) (Six-base) MAPSORT of: pFN21AB4367.seq Check: 3857 from: 1 to: 8077 pFN21AB4367 8077 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5887 278 Size: 53 83 186 936 4351 2468 Fragments arranged by size: 4351 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5420 1937 Size: 3483 4594 Acc65I G'GTAC_C Cuts at: 5398 5398 Size: 8077 AclI AA'CG_TT Cuts at: 1563 2061 6204 6577 1563 Size: 498 4143 373 3063 Fragments arranged by size: 4143 3063 498 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2313 2454 3072 5292 5376 6140 7524 7792 Size: 681 141 618 2220 84 764 1384 268 Cuts at: 7792 1632 Size: 1917 Fragments arranged by size: 2220 1917 1384 764 681 618 268 141 84 AfeI AGC'GCT Cuts at: 4488 4907 4488 Size: 419 7658 AflII C'TTAA_G Cuts at: 820 1017 2779 4480 820 Size: 197 1762 1701 4417 Fragments arranged by size: 4417 1762 1701 197 AflIII A'CryG_T Cuts at: 1203 2692 6976 7953 1203 Size: 1489 4284 977 1327 Fragments arranged by size: 4284 1489 1327 977 AgeI A'CCGG_T Cuts at: 6908 6908 Size: 8077 AhdI GACnn_n'nnGTC Cuts at: 6806 6806 Size: 8077 AleI CACnn'nnGTG Cuts at: 1166 4445 5037 1166 Size: 3279 592 4206 Fragments arranged by size: 4206 3279 592 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5055 1184 Size: 3871 4206 AlwNI CAG_nnn'CTG Cuts at: 3701 3734 4427 5012 5820 7392 3701 Size: 33 693 585 808 1572 4386 Fragments arranged by size: 4386 1572 808 693 585 33 ApaLI G'TGCA_C Cuts at: 4179 6134 7290 4179 Size: 1955 1156 4966 Fragments arranged by size: 4966 1955 1156 ApoI r'AATT_y Cuts at: 1493 2092 2720 2784 3065 3475 5386 5612 5764 Size: 599 628 64 281 410 1911 226 152 Cuts at: 5764 7873 1493 Size: 2109 1697 Fragments arranged by size: 2109 1911 1697 628 599 410 281 226 152 64 AseI AT'TA_AT Cuts at: 160 1032 3627 6631 160 Size: 872 2595 3004 1606 Fragments arranged by size: 3004 2595 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8077 AvaI C'yCGr_G Cuts at: 1870 1943 5402 1870 Size: 73 3459 4545 Fragments arranged by size: 4545 3459 73 BamHI G'GATC_C Cuts at: 5407 5774 5407 Size: 367 7710 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2428 5039 5398 6853 618 Size: 325 217 627 641 2611 359 1455 1842 Fragments arranged by size: 2611 1842 1455 641 627 359 325 217 BanII G_rGCy'C Cuts at: 1391 3837 5396 1391 Size: 2446 1559 4072 Fragments arranged by size: 4072 2446 1559 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2118 3380 3590 3918 4703 5112 928 Size: 971 219 1262 210 328 785 409 3893 Fragments arranged by size: 3893 1262 971 785 409 328 219 210 BbvCI CC'TCA_GC Cuts at: 4173 4173 Size: 8077 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2330 2364 6268 6302 1903 Size: 34 393 34 3904 34 3678 Fragments arranged by size: 3904 3678 393 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5969 7179 5969 Size: 1210 6867 BclI T'GATC_A Cuts at: 1548 3400 3982 1548 Size: 1852 582 5643 Fragments arranged by size: 5643 1852 582 BfrBI ATG'CAT Cuts at: 2498 2498 Size: 8077 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6688 136 Size: 107 122 71 631 605 5016 1525 Fragments arranged by size: 5016 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3535 8072 3535 Size: 4537 3540 BlpI GC'TnA_GC Cuts at: 5494 5494 Size: 8077 Bme1580I G_kGCm'C Cuts at: 1790 2456 4183 6138 7294 1790 Size: 666 1727 1955 1156 2573 Fragments arranged by size: 2573 1955 1727 1156 666 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8077 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4058 4426 5808 Size: 416 53 83 399 24 2641 368 1382 Cuts at: 5808 6766 442 Size: 958 1753 Fragments arranged by size: 2641 1753 1382 958 416 399 368 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4493 1056 Size: 3437 4640 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3756 4890 6737 1239 Size: 2517 1134 1847 2579 Fragments arranged by size: 2579 2517 1847 1134 Bpu10I CC'TnA_GC Cuts at: 2298 4173 5317 2298 Size: 1875 1144 5058 Fragments arranged by size: 5058 1875 1144 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2906 3461 6202 7067 7365 2906 Size: 555 2741 865 298 3618 Fragments arranged by size: 3618 2741 865 555 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6740 882 Size: 617 5241 2219 Fragments arranged by size: 5241 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5035 5868 493 Size: 711 3831 833 2702 Fragments arranged by size: 3831 2702 833 711 BsaBI GATnn'nnATC Cuts at: 8071 8071 Size: 8077 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5048 5884 6266 6901 Size: 53 83 186 936 3515 836 382 635 Cuts at: 6901 275 Size: 1451 Fragments arranged by size: 3515 1451 936 836 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6510 6908 7182 7329 7740 6510 Size: 398 274 147 411 6847 Fragments arranged by size: 6847 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2206 2236 3145 3175 3661 Size: 30 165 30 803 30 909 30 486 Cuts at: 3661 3691 1178 Size: 30 5564 Fragments arranged by size: 5564 909 803 486 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2230 3153 3669 5141 5144 5213 5216 5219 Size: 1044 923 516 1472 3 69 3 3 Cuts at: 5219 5222 1186 Size: 3 4041 Fragments arranged by size: 4041 1472 1044 923 516 69 3 3 3 3 BseYI C'CCAG_C Cuts at: 2250 7280 7776 2250 Size: 5030 496 2551 Fragments arranged by size: 5030 2551 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2638 3846 3941 1163 Size: 573 902 1208 95 5299 Fragments arranged by size: 5299 1208 902 573 95 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5527 6288 7316 1540 Size: 275 186 3526 761 1028 2301 Fragments arranged by size: 3526 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2778 3837 4183 5034 6138 6223 7294 2778 Size: 1059 346 851 1104 85 1071 3561 Fragments arranged by size: 3561 1104 1071 1059 851 346 85 BsmI GAATG_Cn' Cuts at: 2242 2342 2816 3817 5588 5681 2242 Size: 100 474 1001 1771 93 4638 Fragments arranged by size: 4638 1771 1001 474 100 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2952 5055 2952 Size: 2103 5974 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2456 2778 3837 4183 5034 5396 6138 Size: 399 666 322 1059 346 851 362 742 Cuts at: 6138 6223 7294 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1059 851 742 666 399 362 346 322 85 BspEI T'CCGG_A Cuts at: 7740 7740 Size: 8077 BspHI T'CATG_A Cuts at: 2473 2581 4798 5966 7696 2473 Size: 108 2217 1168 1730 2854 Fragments arranged by size: 2854 2217 1730 1168 108 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2193 5432 844 Size: 1349 3239 3489 Fragments arranged by size: 3489 3239 1349 BsrBI CCG'CTC Cuts at: 1394 1823 5964 1394 Size: 429 4141 3507 Fragments arranged by size: 4141 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4452 6572 6746 65 Size: 1183 3204 2120 174 1396 Fragments arranged by size: 3204 2120 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6721 6908 1700 Size: 110 4911 187 2869 Fragments arranged by size: 4911 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2318 4164 96 Size: 2222 1846 4009 Fragments arranged by size: 4009 2222 1846 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8077 BssSI C'ACGA_G Cuts at: 6137 7149 6137 Size: 1012 7065 BstAPI GCAn_nnn'nTGC Cuts at: 794 3803 794 Size: 3009 5068 BstBI TT'CG_AA Cuts at: 6881 6881 Size: 8077 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8077 BstXI CCAn_nnnn'nTGG Cuts at: 4447 4595 4447 Size: 148 7929 BstYI r'GATC_y Cuts at: 1972 3535 5407 5774 6159 6176 7628 7714 7726 Size: 1563 1872 367 385 17 1452 86 12 Cuts at: 7726 8072 1972 Size: 346 1977 Fragments arranged by size: 1977 1872 1563 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2424 3165 2424 Size: 741 7336 BtgI C'CryG_G Cuts at: 513 1065 2004 2048 513 Size: 552 939 44 6542 Fragments arranged by size: 6542 939 552 44 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4931 487 Size: 683 56 3705 3633 Fragments arranged by size: 3705 3633 683 56 BtsI GCAGTG_nn' Cuts at: 2227 2406 4425 5593 6387 6407 2227 Size: 179 2019 1168 794 20 3897 Fragments arranged by size: 3897 2019 1168 794 179 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8077 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8042 DraI TTT'AAA Cuts at: 3480 3771 4828 5381 3480 Size: 291 1057 553 6176 Fragments arranged by size: 6176 1057 553 291 DraIII CAC_nnn'GTG Cuts at: 2736 4391 2736 Size: 1655 6422 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2411 7084 809 Size: 507 1095 4673 1802 Fragments arranged by size: 4673 1802 1095 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3361 4449 5524 Size: 54 1425 50 275 18 1531 1088 1075 Cuts at: 5524 6413 8042 8 Size: 889 1629 43 Fragments arranged by size: 1629 1531 1425 1088 1075 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5524 1537 Size: 275 3712 4090 Fragments arranged by size: 4090 3712 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2379 2430 2674 3825 4182 4995 5091 5118 Size: 1026 51 244 1151 357 813 96 27 Cuts at: 5118 5190 5223 5232 6007 1353 Size: 72 33 9 775 3423 Fragments arranged by size: 3423 1151 1026 813 775 357 244 96 72 51 33 27 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 6664 7038 7184 387 Size: 1064 138 435 4640 374 146 1280 Fragments arranged by size: 4640 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 3835 3835 Size: 8077 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2313 2454 3072 3756 4890 5292 5376 Size: 393 681 141 618 684 1134 402 84 Cuts at: 5376 6140 6737 7524 7792 1239 Size: 764 597 787 268 1524 Fragments arranged by size: 1524 1134 787 764 684 681 618 597 402 393 268 141 84 EcoNI CCTnn'n_nnAGG Cuts at: 3948 3948 Size: 8077 EcoO109I rG'GnC_Cy Cuts at: 2288 5009 2288 Size: 2721 5356 EcoRI G'AATT_C Cuts at: 2784 3065 5386 5764 2784 Size: 281 2321 378 5097 Fragments arranged by size: 5097 2321 378 281 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2848 3041 3326 3796 2848 Size: 193 285 470 7129 Fragments arranged by size: 7129 470 285 193 FspI TGC'GCA Cuts at: 4321 6583 4321 Size: 2262 5815 HaeII r_GCGC'y Cuts at: 1678 4490 4909 7224 1678 Size: 2812 419 2315 2531 Fragments arranged by size: 2812 2531 2315 419 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4610 4642 5336 5368 6718 Size: 32 135 32 3038 32 694 32 1350 Cuts at: 6718 6750 6792 6824 1373 Size: 32 42 32 2626 Fragments arranged by size: 3038 2626 1350 694 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3034 4254 5421 5667 669 Size: 988 281 1096 1220 1167 246 3079 Fragments arranged by size: 3079 1220 1167 1096 988 281 246 HindIII A'AGCT_T Cuts at: 748 2745 3565 748 Size: 1997 820 5260 Fragments arranged by size: 5260 1997 820 HpaI GTT'AAC Cuts at: 3034 5667 3034 Size: 2633 5444 KpnI G_GTAC'C Cuts at: 5402 5402 Size: 8077 MfeI C'AATT_G Cuts at: 5676 5676 Size: 8077 MluI A'CGCG_T Cuts at: 7953 7953 Size: 8077 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3270 7191 7375 1305 Size: 1965 3921 184 2007 Fragments arranged by size: 3921 2007 1965 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3363 4451 10 Size: 54 1425 343 1531 1088 3636 Fragments arranged by size: 3636 1531 1425 1088 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2809 2932 4445 4773 4803 5037 6035 Size: 648 1643 123 1513 328 30 234 998 Cuts at: 6035 6394 6553 518 Size: 359 159 2042 Fragments arranged by size: 2042 1643 1513 998 648 359 328 234 159 123 30 MspA1I CmG'CkG Cuts at: 1606 2050 2156 4172 5493 6170 7318 7563 1606 Size: 444 106 2016 1321 677 1148 245 2120 Fragments arranged by size: 2120 2016 1321 1148 677 444 245 106 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6586 Fragments arranged by size: 6586 939 552 NdeI CA'TA_TG Cuts at: 387 2806 387 Size: 2419 5658 NheI G'CTAG_C Cuts at: 1052 4489 1052 Size: 3437 4640 NotI GC'GGCC_GC Cuts at: 5524 5524 Size: 8077 NsiI A_TGCA'T Cuts at: 2500 2500 Size: 8077 NspI r_CATG'y Cuts at: 1132 1229 2696 4858 5435 6980 1132 Size: 97 1467 2162 577 1545 2229 Fragments arranged by size: 2229 2162 1545 1467 577 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8077 PciI A'CATG_T Cuts at: 2692 6976 2692 Size: 4284 3793 PflMI CCAn_nnn'nTGG Cuts at: 1108 3200 1108 Size: 2092 5985 PfoI T'CCnGG_A Cuts at: 3616 4293 3616 Size: 677 7400 PmeI GTTT'AAAC Cuts at: 5381 5381 Size: 8077 PmlI CAC'GTG Cuts at: 5035 5035 Size: 8077 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6146 6178 7659 7691 6146 Size: 32 1481 32 6532 Fragments arranged by size: 6532 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8077 PsiI TTA'TAA Cuts at: 2073 5647 2073 Size: 3574 4503 PstI C_TGCA'G Cuts at: 830 3694 4561 5429 830 Size: 2864 867 868 3478 Fragments arranged by size: 3478 2864 868 867 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8077 PvuII CAG'CTG Cuts at: 2156 4172 2156 Size: 2016 6061 SacI G_AGCT'C Cuts at: 3837 3837 Size: 8077 SacII CC_GC'GG Cuts at: 2051 2051 Size: 8077 SalI G'TCGA_C Cuts at: 1936 5419 1936 Size: 3483 4594 SapI GCTCTTCn'nnn_ Cuts at: 3825 4182 3825 Size: 357 7720 SbfI CC_TGCA'GG Cuts at: 5429 5429 Size: 8077 ScaI AGT'ACT Cuts at: 6325 8037 6325 Size: 1712 6365 SfcI C'TryA_G Cuts at: 826 1045 2851 3433 3690 4419 4557 5425 6560 Size: 219 1806 582 257 729 138 868 1135 Cuts at: 6560 7241 7432 826 Size: 681 191 1471 Fragments arranged by size: 1806 1471 1135 868 729 681 582 257 219 191 138 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8077 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8077 SmaI CCC'GGG Cuts at: 1872 5404 1872 Size: 3532 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2779 2921 3440 4480 6181 7082 Size: 197 926 836 142 519 1040 1701 901 Cuts at: 7082 7344 820 Size: 262 1553 Fragments arranged by size: 1701 1553 1040 926 901 836 519 262 197 142 SnaBI TAC'GTA Cuts at: 493 5868 493 Size: 5375 2702 SpeI A'CTAG_T Cuts at: 152 4262 152 Size: 4110 3967 SphI G_CATG'C Cuts at: 1132 5435 1132 Size: 4303 3774 SspI AAT'ATT Cuts at: 5 52 6001 5 Size: 47 5949 2081 Fragments arranged by size: 5949 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8077 StyI C'CwwG_G Cuts at: 513 1065 2004 5516 513 Size: 552 939 3512 3074 Fragments arranged by size: 3512 3074 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2697 3361 5027 6104 6121 6274 6459 Size: 1255 1117 664 1666 1077 17 153 185 Cuts at: 6459 6904 325 Size: 445 1498 Fragments arranged by size: 1666 1498 1255 1117 1077 664 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2318 3247 4106 Size: 274 80 33 51 1443 341 929 859 Cuts at: 4106 4164 6323 8035 96 Size: 58 2159 1712 138 Fragments arranged by size: 2159 1712 1443 929 859 341 274 138 80 58 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2206 2238 8037 8069 2206 Size: 32 5799 32 2214 Fragments arranged by size: 5799 2214 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2701 1316 Size: 1385 6692 XbaI T'CTAG_A Cuts at: 5413 5413 Size: 8077 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8077 XmaI C'CCGG_G Cuts at: 1870 5402 1870 Size: 3532 4545 XmnI GAAnn'nnTTC Cuts at: 2388 6206 2388 Size: 3818 4259 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5885 276 Size: 53 83 186 936 4351 2468 Fragments arranged by size: 4351 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI