(Circular) (Six-base) MAPSORT of: pFN21AB4366.seq Check: 4633 from: 1 to: 7474 pFN21AB4366 7474 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3664 3786 3664 Size: 122 7352 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5284 278 Size: 53 83 186 936 3748 2468 Fragments arranged by size: 3748 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4817 1937 Size: 2880 4594 Acc65I G'GTAC_C Cuts at: 3890 4795 3890 Size: 905 6569 AclI AA'CG_TT Cuts at: 1563 5601 5974 1563 Size: 4038 373 3063 Fragments arranged by size: 4038 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2190 4581 5537 6921 7189 1632 Size: 558 2391 956 1384 268 1917 Fragments arranged by size: 2391 1917 1384 956 558 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7277 AflIII A'CryG_T Cuts at: 1203 3656 6373 7350 1203 Size: 2453 2717 977 1327 Fragments arranged by size: 2717 2453 1327 977 AgeI A'CCGG_T Cuts at: 6305 6305 Size: 7474 AhdI GACnn_n'nnGTC Cuts at: 6203 6203 Size: 7474 AleI CACnn'nnGTG Cuts at: 1166 3770 1166 Size: 2604 4870 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7474 AlwNI CAG_nnn'CTG Cuts at: 5217 6789 5217 Size: 1572 5902 ApaLI G'TGCA_C Cuts at: 3979 5531 6687 3979 Size: 1552 1156 4766 Fragments arranged by size: 4766 1552 1156 ApoI r'AATT_y Cuts at: 1493 2389 3174 3200 4783 5009 5161 7270 1493 Size: 896 785 26 1583 226 152 2109 1697 Fragments arranged by size: 2109 1697 1583 896 785 226 152 26 AseI AT'TA_AT Cuts at: 160 1032 2441 6028 160 Size: 872 1409 3587 1606 Fragments arranged by size: 3587 1606 1409 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7474 AvaI C'yCGr_G Cuts at: 1870 1943 2226 4048 4799 1870 Size: 73 283 1822 751 4545 Fragments arranged by size: 4545 1822 751 283 73 BamHI G'GATC_C Cuts at: 4804 5171 4804 Size: 367 7107 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3890 4795 6250 618 Size: 325 217 627 2103 905 1455 1842 Fragments arranged by size: 2103 1842 1455 905 627 325 217 BanII G_rGCy'C Cuts at: 1391 3297 4793 1391 Size: 1906 1496 4072 Fragments arranged by size: 4072 1906 1496 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4641 928 Size: 971 2742 3761 Fragments arranged by size: 3761 2742 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5665 5699 1903 Size: 34 3728 34 3678 Fragments arranged by size: 3728 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4293 4456 5366 6576 4293 Size: 163 910 1210 5191 Fragments arranged by size: 5191 1210 910 163 BclI T'GATC_A Cuts at: 1548 3478 1548 Size: 1930 5544 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6085 136 Size: 107 122 71 631 605 4413 1525 Fragments arranged by size: 4413 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3826 7469 3826 Size: 3643 3831 BlpI GC'TnA_GC Cuts at: 4891 4891 Size: 7474 Bme1580I G_kGCm'C Cuts at: 1790 3983 4327 5535 6691 1790 Size: 2193 344 1208 1156 2573 Fragments arranged by size: 2573 2193 1208 1156 344 BmgBI CAC'GTC Cuts at: 1318 2857 1318 Size: 1539 5935 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4081 4302 5205 Size: 416 53 83 399 24 2664 221 903 Cuts at: 5205 6163 442 Size: 958 1753 Fragments arranged by size: 2664 1753 958 903 416 399 221 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7474 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2633 3163 3267 3426 4619 4763 6134 1239 Size: 1394 530 104 159 1193 144 1371 2579 Fragments arranged by size: 2579 1394 1371 1193 530 159 144 104 Bpu10I CC'TnA_GC Cuts at: 3663 3663 Size: 7474 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2105 2188 5599 6464 6762 2105 Size: 83 3411 865 298 2817 Fragments arranged by size: 3411 2817 865 298 83 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4665 6137 882 Size: 617 3166 1472 2219 Fragments arranged by size: 3166 2219 1472 617 BsaAI yAC'GTr Cuts at: 493 1204 5265 493 Size: 711 4061 2702 Fragments arranged by size: 4061 2702 711 BsaBI GATnn'nnATC Cuts at: 3444 3450 3753 7468 3444 Size: 6 303 3715 3450 Fragments arranged by size: 3715 3450 303 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2155 5281 5663 6298 Size: 53 83 186 936 622 3126 382 635 Cuts at: 6298 275 Size: 1451 Fragments arranged by size: 3126 1451 936 635 622 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5907 6305 6579 6726 7137 5907 Size: 398 274 147 411 6244 Fragments arranged by size: 6244 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4516 4546 1178 Size: 30 165 30 3113 30 4106 Fragments arranged by size: 4106 3113 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2738 3423 4661 4664 1186 Size: 1552 685 1238 3 3996 Fragments arranged by size: 3996 1552 1238 685 3 BseYI C'CCAG_C Cuts at: 6677 7173 6677 Size: 496 6978 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3227 3765 1163 Size: 573 1491 538 4872 Fragments arranged by size: 4872 1491 573 538 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2530 2673 4112 4924 5685 6713 Size: 275 186 529 143 1439 812 761 1028 Cuts at: 6713 1540 Size: 2301 Fragments arranged by size: 2301 1439 1028 812 761 529 275 186 143 BsiHKAI G_wGCw'C Cuts at: 3664 3983 5535 5620 6691 3664 Size: 319 1552 85 1071 4447 Fragments arranged by size: 4447 1552 1071 319 85 BsmI GAATG_Cn' Cuts at: 3218 4985 5078 3218 Size: 1767 93 5614 Fragments arranged by size: 5614 1767 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4492 4492 Size: 7474 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3297 3664 3983 4327 4793 5535 5620 Size: 399 1507 367 319 344 466 742 85 Cuts at: 5620 6691 1391 Size: 1071 2174 Fragments arranged by size: 2174 1507 1071 742 466 399 367 344 319 85 BspEI T'CCGG_A Cuts at: 7137 7137 Size: 7474 BspHI T'CATG_A Cuts at: 3352 3442 3913 5363 7093 3352 Size: 90 471 1450 1730 3733 Fragments arranged by size: 3733 1730 1450 471 90 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3664 3786 4829 844 Size: 2820 122 1043 3489 Fragments arranged by size: 3489 2820 1043 122 BsrBI CCG'CTC Cuts at: 1394 1823 3147 5361 1394 Size: 429 1324 2214 3507 Fragments arranged by size: 3507 2214 1324 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2691 5969 6143 65 Size: 1183 1443 3278 174 1396 Fragments arranged by size: 3278 1443 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2018 6118 6305 1700 Size: 110 208 4100 187 2869 Fragments arranged by size: 4100 2869 208 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7474 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7474 BssSI C'ACGA_G Cuts at: 2091 5534 6546 2091 Size: 3443 1012 3019 Fragments arranged by size: 3443 3019 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7474 BstBI TT'CG_AA Cuts at: 6278 6278 Size: 7474 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7474 BstXI CCAn_nnnn'nTGG Cuts at: 4597 4741 4597 Size: 144 7330 BstYI r'GATC_y Cuts at: 1972 3826 3908 4187 4804 5171 5556 5573 7025 Size: 1854 82 279 617 367 385 17 1452 Cuts at: 7025 7111 7123 7469 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1854 1452 617 385 367 346 279 86 82 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5983 Fragments arranged by size: 5983 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6735 Fragments arranged by size: 6735 683 56 BtsI GCAGTG_nn' Cuts at: 2888 3215 3367 4990 5784 5804 2888 Size: 327 152 1623 794 20 4558 Fragments arranged by size: 4558 1623 794 327 152 20 ClaI AT'CG_AT Cuts at: 1554 4108 1554 Size: 2554 4920 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2245 2280 529 Size: 35 1681 35 5723 Fragments arranged by size: 5723 1681 35 35 DraI TTT'AAA Cuts at: 4778 4778 Size: 7474 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6481 809 Size: 507 5165 1802 Fragments arranged by size: 5165 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2531 3774 4178 Size: 54 1425 50 275 18 701 1243 404 Cuts at: 4178 4921 5810 7439 8 Size: 743 889 1629 43 Fragments arranged by size: 1629 1425 1243 889 743 701 404 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4921 1537 Size: 275 3109 4090 Fragments arranged by size: 4090 3109 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4495 4557 4653 4689 5404 1353 Size: 3142 62 96 36 715 3423 Fragments arranged by size: 3423 3142 715 96 62 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6061 6435 6581 387 Size: 1064 138 4472 374 146 1280 Fragments arranged by size: 4472 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2190 2633 3163 3267 3426 4581 4619 Size: 393 558 443 530 104 159 1155 38 Cuts at: 4619 4763 5537 6134 6921 7189 1239 Size: 144 774 597 787 268 1524 Fragments arranged by size: 1524 1155 787 774 597 558 530 443 393 268 159 144 104 38 EcoNI CCTnn'n_nnAGG Cuts at: 3306 4742 3306 Size: 1436 6038 EcoO109I rG'GnC_Cy Cuts at: 3311 3311 Size: 7474 EcoRI G'AATT_C Cuts at: 4783 5161 4783 Size: 378 7096 EcoRV GAT'ATC Cuts at: 4204 4380 4204 Size: 176 7298 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2339 4754 2339 Size: 2415 5059 FspI TGC'GCA Cuts at: 5980 5980 Size: 7474 HaeII r_GCGC'y Cuts at: 1678 6621 1678 Size: 4943 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2092 3646 3678 6115 Size: 32 135 32 488 32 1554 32 2437 Cuts at: 6115 6147 6189 6221 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2437 1554 488 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2563 4818 5064 669 Size: 988 281 625 2255 246 3079 Fragments arranged by size: 3079 2255 988 625 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7474 HpaI GTT'AAC Cuts at: 5064 5064 Size: 7474 KpnI G_GTAC'C Cuts at: 3894 4799 3894 Size: 905 6569 MfeI C'AATT_G Cuts at: 2304 5073 2304 Size: 2769 4705 MluI A'CGCG_T Cuts at: 7350 7350 Size: 7474 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2693 6588 6772 1305 Size: 1388 3895 184 2007 Fragments arranged by size: 3895 2007 1388 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3776 4180 10 Size: 54 1425 343 1944 404 3304 Fragments arranged by size: 3304 1944 1425 404 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2292 2478 2970 3606 3759 3770 5432 Size: 648 1126 186 492 636 153 11 1662 Cuts at: 5432 5791 5950 518 Size: 359 159 2042 Fragments arranged by size: 2042 1662 1126 648 636 492 359 186 159 153 11 MspA1I CmG'CkG Cuts at: 1606 2363 4559 4668 4890 5567 6715 6960 1606 Size: 757 2196 109 222 677 1148 245 2120 Fragments arranged by size: 2196 2120 1148 757 677 245 222 109 NaeI GCC'GGC Cuts at: 2020 2020 Size: 7474 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5983 Fragments arranged by size: 5983 939 552 NdeI CA'TA_TG Cuts at: 387 2623 2997 387 Size: 2236 374 4864 Fragments arranged by size: 4864 2236 374 NgoMIV G'CCGG_C Cuts at: 2018 2018 Size: 7474 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7474 NotI GC'GGCC_GC Cuts at: 4921 4921 Size: 7474 NspI r_CATG'y Cuts at: 1132 1229 3114 3660 4832 6377 1132 Size: 97 1885 546 1172 1545 2229 Fragments arranged by size: 2229 1885 1545 1172 546 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7474 PciI A'CATG_T Cuts at: 3656 6373 3656 Size: 2717 4757 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7474 PmeI GTTT'AAAC Cuts at: 4778 4778 Size: 7474 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5543 5575 7056 7088 5543 Size: 32 1481 32 5929 Fragments arranged by size: 5929 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3311 3311 Size: 7474 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7474 PsiI TTA'TAA Cuts at: 5044 5044 Size: 7474 PspXI vC'TCGA_Gb Cuts at: 2226 2226 Size: 7474 PstI C_TGCA'G Cuts at: 830 2467 2895 4826 830 Size: 1637 428 1931 3478 Fragments arranged by size: 3478 1931 1637 428 PvuI CG_AT'CG Cuts at: 2001 4112 2001 Size: 2111 5363 PvuII CAG'CTG Cuts at: 2363 4559 2363 Size: 2196 5278 SalI G'TCGA_C Cuts at: 1936 4816 1936 Size: 2880 4594 SapI GCTCTTCn'nnn_ Cuts at: 4689 4689 Size: 7474 SbfI CC_TGCA'GG Cuts at: 4826 4826 Size: 7474 ScaI AGT'ACT Cuts at: 2053 2709 5722 7434 2053 Size: 656 3013 1712 2093 Fragments arranged by size: 3013 2093 1712 656 SfcI C'TryA_G Cuts at: 826 1045 2463 2644 2891 3288 3501 3903 4455 Size: 219 1418 181 247 397 213 402 552 Cuts at: 4455 4822 5957 6638 6829 826 Size: 367 1135 681 191 1471 Fragments arranged by size: 1471 1418 1135 681 552 402 397 367 247 219 213 191 181 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7474 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7474 SmaI CCC'GGG Cuts at: 1872 4801 1872 Size: 2929 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2084 2203 2226 5578 6479 6741 Size: 197 926 141 119 23 3352 901 262 Cuts at: 6741 820 Size: 1553 Fragments arranged by size: 3352 1553 926 901 262 197 141 119 23 SnaBI TAC'GTA Cuts at: 493 5265 493 Size: 4772 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7474 SphI G_CATG'C Cuts at: 1132 4832 1132 Size: 3700 3774 SspI AAT'ATT Cuts at: 5 52 4065 5398 5 Size: 47 4013 1333 2081 Fragments arranged by size: 4013 2081 1333 47 StuI AGG'CCT Cuts at: 1517 2842 1517 Size: 1325 6149 StyI C'CwwG_G Cuts at: 513 1065 2004 4675 4913 513 Size: 552 939 2671 238 3074 Fragments arranged by size: 3074 2671 939 552 238 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2449 3099 5501 5518 5671 5856 6301 Size: 1255 869 650 2402 17 153 185 445 Cuts at: 6301 325 Size: 1498 Fragments arranged by size: 2402 1498 1255 869 650 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2051 2707 3592 Size: 274 80 33 51 1443 74 656 885 Cuts at: 3592 4068 4317 5720 7432 96 Size: 476 249 1403 1712 138 Fragments arranged by size: 1712 1443 1403 885 656 476 274 249 138 80 74 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4516 4548 7434 7466 4516 Size: 32 2886 32 4524 Fragments arranged by size: 4524 2886 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7474 XbaI T'CTAG_A Cuts at: 4810 4810 Size: 7474 XhoI C'TCGA_G Cuts at: 1943 2226 1943 Size: 283 7191 XmaI C'CCGG_G Cuts at: 1870 4799 1870 Size: 2929 4545 XmnI GAAnn'nnTTC Cuts at: 5603 5603 Size: 7474 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5282 276 Size: 53 83 186 936 3748 2468 Fragments arranged by size: 3748 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI