(Circular) (Six-base) MAPSORT of: pFN21AB4351.seq Check: 474 from: 1 to: 5785 pFN21AB4351 5785 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2044 2044 Size: 5785 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3595 278 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3128 1937 Size: 1191 4594 Acc65I G'GTAC_C Cuts at: 3106 3106 Size: 5785 AclI AA'CG_TT Cuts at: 1563 2436 3912 4285 1563 Size: 873 1476 373 3063 Fragments arranged by size: 3063 1476 873 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2221 3848 5232 5500 1632 Size: 589 1627 1384 268 1917 Fragments arranged by size: 1917 1627 1384 589 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5588 AflIII A'CryG_T Cuts at: 1203 2573 4684 5661 1203 Size: 1370 2111 977 1327 Fragments arranged by size: 2111 1370 1327 977 AgeI A'CCGG_T Cuts at: 4616 4616 Size: 5785 AhdI GACnn_n'nnGTC Cuts at: 4514 4514 Size: 5785 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5785 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5785 AlwNI CAG_nnn'CTG Cuts at: 2926 3528 5100 2926 Size: 602 1572 3611 Fragments arranged by size: 3611 1572 602 ApaI G_GGCC'C Cuts at: 2036 2036 Size: 5785 ApaLI G'TGCA_C Cuts at: 3842 4998 3842 Size: 1156 4629 ApoI r'AATT_y Cuts at: 1493 2605 2773 3094 3320 3472 5581 1493 Size: 1112 168 321 226 152 2109 1697 Fragments arranged by size: 2109 1697 1112 321 226 168 152 AseI AT'TA_AT Cuts at: 160 1032 4339 160 Size: 872 3307 1606 Fragments arranged by size: 3307 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 AvaI C'yCGr_G Cuts at: 1870 1943 2959 3110 1870 Size: 73 1016 151 4545 Fragments arranged by size: 4545 1016 151 73 BamHI G'GATC_C Cuts at: 2738 3115 3482 2738 Size: 377 367 5041 Fragments arranged by size: 5041 377 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2105 2821 3106 4561 618 Size: 325 217 627 318 716 285 1455 1842 Fragments arranged by size: 1842 1455 716 627 325 318 285 217 BanII G_rGCy'C Cuts at: 1391 2036 2590 2973 3104 1391 Size: 645 554 383 131 4072 Fragments arranged by size: 4072 645 554 383 131 BbeI G_GCGC'C Cuts at: 2109 2109 Size: 5785 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2360 2760 928 Size: 971 461 400 3953 Fragments arranged by size: 3953 971 461 400 BbvCI CC'TCA_GC Cuts at: 2366 2366 Size: 5785 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3976 4010 1903 Size: 34 2039 34 3678 Fragments arranged by size: 3678 2039 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3068 3677 4887 3068 Size: 609 1210 3966 Fragments arranged by size: 3966 1210 609 BclI T'GATC_A Cuts at: 1548 2539 1548 Size: 991 4794 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4396 136 Size: 107 122 71 631 605 337 2387 1525 Fragments arranged by size: 2387 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2335 2496 3080 5780 2335 Size: 161 584 2700 2340 Fragments arranged by size: 2700 2340 584 161 BlpI GC'TnA_GC Cuts at: 3202 3202 Size: 5785 Bme1580I G_kGCm'C Cuts at: 1790 2036 3846 5002 1790 Size: 246 1810 1156 2573 Fragments arranged by size: 2573 1810 1156 246 BmgBI CAC'GTC Cuts at: 1318 2065 1318 Size: 747 5038 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3516 4474 442 Size: 416 53 83 399 24 2099 958 1753 Fragments arranged by size: 2099 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5785 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2574 2963 4445 1239 Size: 1335 389 1482 2579 Fragments arranged by size: 2579 1482 1335 389 Bpu10I CC'TnA_GC Cuts at: 2366 2366 Size: 5785 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2438 2477 3910 4775 5073 2438 Size: 39 1433 865 298 3150 Fragments arranged by size: 3150 1433 865 298 39 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4448 882 Size: 617 2949 2219 Fragments arranged by size: 2949 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3576 493 Size: 711 2372 2702 Fragments arranged by size: 2702 2372 711 BsaBI GATnn'nnATC Cuts at: 2222 2743 3079 5779 2222 Size: 521 336 2700 2228 Fragments arranged by size: 2700 2228 521 336 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2106 3592 3974 4609 Size: 53 83 186 936 573 1486 382 635 Cuts at: 4609 275 Size: 1451 Fragments arranged by size: 1486 1451 936 635 573 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4218 4616 4890 5037 5448 4218 Size: 398 274 147 411 4555 Fragments arranged by size: 4555 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5560 Fragments arranged by size: 5560 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5785 BseYI C'CCAG_C Cuts at: 4988 5484 4988 Size: 496 5289 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2426 1163 Size: 573 690 4522 Fragments arranged by size: 4522 690 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3235 3996 5024 1540 Size: 275 186 1234 761 1028 2301 Fragments arranged by size: 2301 1234 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2126 2187 2281 3846 3931 5002 2126 Size: 61 94 1565 85 1071 2909 Fragments arranged by size: 2909 1565 1071 94 85 61 BsmI GAATG_Cn' Cuts at: 2652 3296 3389 2652 Size: 644 93 5048 Fragments arranged by size: 5048 644 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2126 2187 2281 2590 2973 3104 Size: 399 246 90 61 94 309 383 131 Cuts at: 3104 3846 3931 5002 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 383 309 246 131 94 90 85 61 BspEI T'CCGG_A Cuts at: 5448 5448 Size: 5785 BspHI T'CATG_A Cuts at: 3674 5404 3674 Size: 1730 4055 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2044 2475 3140 844 Size: 1200 431 665 3489 Fragments arranged by size: 3489 1200 665 431 BsrBI CCG'CTC Cuts at: 1394 1823 3672 1394 Size: 429 1849 3507 Fragments arranged by size: 3507 1849 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2377 4280 4454 65 Size: 1183 1129 1903 174 1396 Fragments arranged by size: 1903 1396 1183 1129 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4429 4616 1700 Size: 110 2619 187 2869 Fragments arranged by size: 2869 2619 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5785 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5785 BssSI C'ACGA_G Cuts at: 3845 4857 3845 Size: 1012 4773 BstAPI GCAn_nnn'nTGC Cuts at: 794 2121 794 Size: 1327 4458 BstBI TT'CG_AA Cuts at: 4589 4589 Size: 5785 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5785 BstXI CCAn_nnnn'nTGG Cuts at: 2863 2863 Size: 5785 BstYI r'GATC_y Cuts at: 1972 2335 2400 2496 2527 2738 3080 3115 3482 Size: 363 65 96 31 211 342 35 367 Cuts at: 3482 3867 3884 5336 5422 5434 5780 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 363 346 342 211 96 86 65 35 31 17 12 Bsu36I CC'TnA_GG Cuts at: 2210 3021 2210 Size: 811 4974 BtgI C'CryG_G Cuts at: 513 1065 2004 2047 2825 513 Size: 552 939 43 778 3473 Fragments arranged by size: 3473 939 778 552 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2959 487 Size: 683 56 1733 3313 Fragments arranged by size: 3313 1733 683 56 BtsI GCAGTG_nn' Cuts at: 3301 4095 4115 3301 Size: 794 20 4971 Fragments arranged by size: 4971 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5785 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5750 DraI TTT'AAA Cuts at: 2778 3089 2778 Size: 311 5474 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4792 809 Size: 507 3476 1802 Fragments arranged by size: 3476 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2568 3232 4121 Size: 54 1425 50 275 18 738 664 889 Cuts at: 4121 5750 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 738 664 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3232 1537 Size: 275 1420 4090 Fragments arranged by size: 4090 1420 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2808 3045 3715 1353 Size: 1455 237 670 3423 Fragments arranged by size: 3423 1455 670 237 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4372 4746 4892 387 Size: 1064 138 2783 374 146 1280 Fragments arranged by size: 2783 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2221 2574 2963 3848 4445 5232 5500 Size: 393 589 353 389 885 597 787 268 Cuts at: 5500 1239 Size: 1524 Fragments arranged by size: 1524 885 787 597 589 393 389 353 268 EcoO109I rG'GnC_Cy Cuts at: 2320 2320 Size: 5785 EcoRI G'AATT_C Cuts at: 2605 3094 3472 2605 Size: 489 378 4918 Fragments arranged by size: 4918 489 378 FspI TGC'GCA Cuts at: 4291 4291 Size: 5785 HaeII r_GCGC'y Cuts at: 1678 2109 4932 1678 Size: 431 2823 2531 Fragments arranged by size: 2823 2531 431 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2145 2177 2624 2656 4426 Size: 32 135 32 573 32 447 32 1770 Cuts at: 4426 4458 4500 4532 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1770 573 447 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2143 2886 3129 3375 669 Size: 988 281 205 743 243 246 3079 Fragments arranged by size: 3079 988 743 281 246 243 205 HindIII A'AGCT_T Cuts at: 748 748 Size: 5785 HpaI GTT'AAC Cuts at: 3375 3375 Size: 5785 KasI G'GCGC_C Cuts at: 2105 2105 Size: 5785 KpnI G_GTAC'C Cuts at: 3110 3110 Size: 5785 MfeI C'AATT_G Cuts at: 3384 3384 Size: 5785 MluI A'CGCG_T Cuts at: 5661 5661 Size: 5785 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2487 4899 5083 1305 Size: 1182 2412 184 2007 Fragments arranged by size: 2412 2007 1182 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2570 10 Size: 54 1425 343 738 3225 Fragments arranged by size: 3225 1425 738 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3743 4102 4261 518 Size: 648 2577 359 159 2042 Fragments arranged by size: 2577 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2049 2713 3201 3878 5026 5271 1606 Size: 443 664 488 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 664 488 443 245 NarI GG'CG_CC Cuts at: 2106 2106 Size: 5785 NcoI C'CATG_G Cuts at: 513 1065 2004 2825 513 Size: 552 939 821 3473 Fragments arranged by size: 3473 939 821 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5785 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5785 NotI GC'GGCC_GC Cuts at: 3232 3232 Size: 5785 NspI r_CATG'y Cuts at: 1132 1229 3143 4688 1132 Size: 97 1914 1545 2229 Fragments arranged by size: 2229 1914 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5785 PciI A'CATG_T Cuts at: 4684 4684 Size: 5785 PflMI CCAn_nnn'nTGG Cuts at: 1108 2577 2725 1108 Size: 1469 148 4168 Fragments arranged by size: 4168 1469 148 PmeI GTTT'AAAC Cuts at: 3089 3089 Size: 5785 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3854 3886 5367 5399 3854 Size: 32 1481 32 4240 Fragments arranged by size: 4240 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2320 2320 Size: 5785 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5785 PsiI TTA'TAA Cuts at: 3355 3355 Size: 5785 PspOMI G'GGCC_C Cuts at: 2032 2032 Size: 5785 PstI C_TGCA'G Cuts at: 830 3137 830 Size: 2307 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5785 PvuII CAG'CTG Cuts at: 2713 2713 Size: 5785 SacII CC_GC'GG Cuts at: 2050 2050 Size: 5785 SalI G'TCGA_C Cuts at: 1936 3127 1936 Size: 1191 4594 SbfI CC_TGCA'GG Cuts at: 3137 3137 Size: 5785 ScaI AGT'ACT Cuts at: 4033 5745 4033 Size: 1712 4073 SfcI C'TryA_G Cuts at: 826 1045 2643 3133 4268 4949 5140 826 Size: 219 1598 490 1135 681 191 1471 Fragments arranged by size: 1598 1471 1135 681 490 219 191 SfoI GGC'GCC Cuts at: 2107 2107 Size: 5785 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5785 SmaI CCC'GGG Cuts at: 1872 3112 1872 Size: 1240 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2453 2492 3889 4790 5052 820 Size: 197 926 510 39 1397 901 262 1553 Fragments arranged by size: 1553 1397 926 901 510 262 197 39 SnaBI TAC'GTA Cuts at: 493 3576 493 Size: 3083 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5785 SphI G_CATG'C Cuts at: 1132 3143 1132 Size: 2011 3774 SspI AAT'ATT Cuts at: 5 52 3709 5 Size: 47 3657 2081 Fragments arranged by size: 3657 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5785 StyI C'CwwG_G Cuts at: 513 1065 2004 2610 2825 3224 513 Size: 552 939 606 215 399 3074 Fragments arranged by size: 3074 939 606 552 399 215 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2949 3812 3829 3982 4167 4612 325 Size: 1255 1369 863 17 153 185 445 1498 Fragments arranged by size: 1498 1369 1255 863 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2314 4031 5743 Size: 274 80 33 51 1443 337 1717 1712 Cuts at: 5743 96 Size: 138 Fragments arranged by size: 1717 1712 1443 337 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5745 5777 5745 Size: 32 5753 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5785 XbaI T'CTAG_A Cuts at: 3121 3121 Size: 5785 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5785 XmaI C'CCGG_G Cuts at: 1870 3110 1870 Size: 1240 4545 XmnI GAAnn'nnTTC Cuts at: 3914 3914 Size: 5785 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3593 276 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI