(Circular) (Six-base) MAPSORT of: pFN21AB4303.seq Check: 8770 from: 1 to: 6610 pFN21AB4303 6610 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3219 3743 3219 Size: 524 6086 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4420 278 Size: 53 83 186 936 2884 2468 Fragments arranged by size: 2884 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3953 1937 Size: 2016 4594 Acc65I G'GTAC_C Cuts at: 3931 3931 Size: 6610 AclI AA'CG_TT Cuts at: 1563 4737 5110 1563 Size: 3174 373 3063 Fragments arranged by size: 3174 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2276 2378 3387 4673 6057 6325 1632 Size: 644 102 1009 1286 1384 268 1917 Fragments arranged by size: 1917 1384 1286 1009 644 268 102 AflII C'TTAA_G Cuts at: 820 1017 2216 820 Size: 197 1199 5214 Fragments arranged by size: 5214 1199 197 AflIII A'CryG_T Cuts at: 1203 2397 3313 3565 5509 6486 1203 Size: 1194 916 252 1944 977 1327 Fragments arranged by size: 1944 1327 1194 977 916 252 AgeI A'CCGG_T Cuts at: 5441 5441 Size: 6610 AhdI GACnn_n'nnGTC Cuts at: 2746 5339 2746 Size: 2593 4017 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6610 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6610 AlwNI CAG_nnn'CTG Cuts at: 3386 3404 3472 4353 5925 3386 Size: 18 68 881 1572 4071 Fragments arranged by size: 4071 1572 881 68 18 ApaI G_GGCC'C Cuts at: 2805 2805 Size: 6610 ApaLI G'TGCA_C Cuts at: 2591 2609 4667 5823 2591 Size: 18 2058 1156 3378 Fragments arranged by size: 3378 2058 1156 18 ApoI r'AATT_y Cuts at: 1493 3919 4145 4297 6406 1493 Size: 2426 226 152 2109 1697 Fragments arranged by size: 2426 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5164 160 Size: 872 4132 1606 Fragments arranged by size: 4132 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6610 AvaI C'yCGr_G Cuts at: 1870 1943 2753 2804 2964 3642 3935 1870 Size: 73 810 51 160 678 293 4545 Fragments arranged by size: 4545 810 678 293 160 73 51 AvrII C'CTAG_G Cuts at: 2078 3757 2078 Size: 1679 4931 BamHI G'GATC_C Cuts at: 3652 3940 4307 3652 Size: 288 367 5955 Fragments arranged by size: 5955 367 288 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2732 3113 3875 3931 5386 Size: 325 217 627 945 381 762 56 1455 Cuts at: 5386 618 Size: 1842 Fragments arranged by size: 1842 1455 945 762 627 381 325 217 56 BanII G_rGCy'C Cuts at: 1391 2202 2805 3156 3339 3432 3929 1391 Size: 811 603 351 183 93 497 4072 Fragments arranged by size: 4072 811 603 497 351 183 93 BbeI G_GCGC'C Cuts at: 3879 3879 Size: 6610 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5639 BbvCI CC'TCA_GC Cuts at: 3405 3405 Size: 6610 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4801 4835 1903 Size: 34 2864 34 3678 Fragments arranged by size: 3678 2864 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4502 5712 4502 Size: 1210 5400 BclI T'GATC_A Cuts at: 1548 1548 Size: 6610 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2546 5221 136 Size: 107 122 71 631 605 874 2675 1525 Fragments arranged by size: 2675 1525 874 631 605 122 107 71 BglII A'GATC_T Cuts at: 6605 6605 Size: 6610 BlpI GC'TnA_GC Cuts at: 2513 4027 2513 Size: 1514 5096 Bme1580I G_kGCm'C Cuts at: 1790 2152 2595 2613 2735 2805 3090 3700 4671 Size: 362 443 18 122 70 285 610 971 Cuts at: 4671 5827 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 971 610 443 362 285 122 70 18 BmgBI CAC'GTC Cuts at: 1318 2398 3568 1318 Size: 1080 1170 4360 Fragments arranged by size: 4360 1170 1080 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3073 3630 4341 Size: 416 53 83 399 24 1656 557 711 Cuts at: 4341 5299 442 Size: 958 1753 Fragments arranged by size: 1753 1656 958 711 557 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6610 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2941 2941 Size: 6610 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2128 3308 3353 3416 3464 5270 1239 Size: 889 1180 45 63 48 1806 2579 Fragments arranged by size: 2579 1806 1180 889 63 48 45 Bpu10I CC'TnA_GC Cuts at: 3405 3473 3405 Size: 68 6542 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2672 3326 4735 5600 5898 2672 Size: 654 1409 865 298 3384 Fragments arranged by size: 3384 1409 865 654 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2882 3077 5273 882 Size: 617 1383 195 2196 2219 Fragments arranged by size: 2219 2196 1383 617 195 BsaAI yAC'GTr Cuts at: 493 1204 2488 2608 3316 4401 493 Size: 711 1284 120 708 1085 2702 Fragments arranged by size: 2702 1284 1085 711 708 120 BsaBI GATnn'nnATC Cuts at: 6604 6604 Size: 6610 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2861 3876 4417 4799 Size: 53 83 186 936 1328 1015 541 382 Cuts at: 4799 5434 275 Size: 635 1451 Fragments arranged by size: 1451 1328 1015 936 635 541 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3033 3100 5043 5441 5715 5862 6273 3033 Size: 67 1943 398 274 147 411 3370 Fragments arranged by size: 3370 1943 411 398 274 147 67 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3158 3188 3218 3248 1178 Size: 30 165 30 1755 30 30 30 4540 Fragments arranged by size: 4540 1755 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2402 2972 3461 3464 1186 Size: 1216 570 489 3 4332 Fragments arranged by size: 4332 1216 570 489 3 BseYI C'CCAG_C Cuts at: 2191 2824 3007 3326 5813 6309 2191 Size: 633 183 319 2487 496 2492 Fragments arranged by size: 2492 2487 633 496 319 183 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2420 1163 Size: 573 684 5353 Fragments arranged by size: 5353 684 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2541 2717 3680 4060 4821 5849 Size: 275 186 540 176 963 380 761 1028 Cuts at: 5849 1540 Size: 2301 Fragments arranged by size: 2301 1028 963 761 540 380 275 186 176 BsiHKAI G_wGCw'C Cuts at: 2595 2613 2922 3432 3459 4671 4756 5827 2595 Size: 18 309 510 27 1212 85 1071 3378 Fragments arranged by size: 3378 1212 1071 510 309 85 27 18 BsmI GAATG_Cn' Cuts at: 4121 4214 4121 Size: 93 6517 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2152 2202 2595 2613 2735 2805 2922 Size: 399 362 50 393 18 122 70 117 Cuts at: 2922 3090 3156 3339 3432 3459 3700 3929 4671 Size: 168 66 183 93 27 241 229 742 Cuts at: 4671 4756 5827 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 393 362 241 229 183 168 122 117 93 85 70 66 50 27 18 BspEI T'CCGG_A Cuts at: 3033 6273 3033 Size: 3240 3370 BspHI T'CATG_A Cuts at: 2337 4499 6229 2337 Size: 2162 1730 2718 Fragments arranged by size: 2718 2162 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2508 3219 3743 3965 844 Size: 1664 711 524 222 3489 Fragments arranged by size: 3489 1664 711 524 222 BsrBI CCG'CTC Cuts at: 1394 1823 2530 3586 4497 1394 Size: 429 707 1056 911 3507 Fragments arranged by size: 3507 1056 911 707 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5105 5279 65 Size: 1183 3857 174 1396 Fragments arranged by size: 3857 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2415 2595 3853 5254 5441 1700 Size: 110 605 180 1258 1401 187 2869 Fragments arranged by size: 2869 1401 1258 605 187 180 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6610 BssHII G'CGCG_C Cuts at: 1925 2549 1925 Size: 624 5986 BssSI C'ACGA_G Cuts at: 3812 4670 5682 3812 Size: 858 1012 4740 Fragments arranged by size: 4740 1012 858 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6610 BstBI TT'CG_AA Cuts at: 3108 5414 3108 Size: 2306 4304 BstEII G'GTnAC_C Cuts at: 1184 2159 1184 Size: 975 5635 BstXI CCAn_nnnn'nTGG Cuts at: 3280 3286 3280 Size: 6 6604 BstYI r'GATC_y Cuts at: 1972 2757 3652 3940 4307 4692 4709 6161 6247 Size: 785 895 288 367 385 17 1452 86 Cuts at: 6247 6259 6605 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 895 785 385 367 346 288 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2955 3248 2955 Size: 293 6317 BtgI C'CryG_G Cuts at: 513 1065 2004 2880 3162 3279 3648 3680 513 Size: 552 939 876 282 117 369 32 3443 Fragments arranged by size: 3443 939 876 552 369 282 117 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5871 Fragments arranged by size: 5871 683 56 BtsI GCAGTG_nn' Cuts at: 2507 3156 4126 4920 4940 2507 Size: 649 970 794 20 4177 Fragments arranged by size: 4177 970 794 649 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6610 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6575 DraI TTT'AAA Cuts at: 3914 3914 Size: 6610 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2948 3260 5617 809 Size: 507 1632 312 2357 1802 Fragments arranged by size: 2357 1802 1632 507 312 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2538 2577 2653 Size: 54 1425 50 275 18 708 39 76 Cuts at: 2653 2667 3677 3855 4057 4946 6575 8 Size: 14 1010 178 202 889 1629 43 Fragments arranged by size: 1629 1425 1010 889 708 275 202 178 76 54 50 43 39 18 14 EagI C'GGCC_G Cuts at: 1537 1812 2538 3677 4057 1537 Size: 275 726 1139 380 4090 Fragments arranged by size: 4090 1139 726 380 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2354 4540 1353 Size: 1001 2186 3423 Fragments arranged by size: 3423 2186 1001 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2579 2651 2900 5197 5571 5717 Size: 1064 138 990 72 249 2297 374 146 Cuts at: 5717 387 Size: 1280 Fragments arranged by size: 2297 1280 1064 990 374 249 146 138 72 EcoICRI GAG'CTC Cuts at: 3430 3430 Size: 6610 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2128 2276 2378 3308 3353 3387 3416 Size: 393 496 148 102 930 45 34 29 Cuts at: 3416 3464 4673 5270 6057 6325 1239 Size: 48 1209 597 787 268 1524 Fragments arranged by size: 1524 1209 930 787 597 496 393 268 148 102 48 45 34 29 EcoNI CCTnn'n_nnAGG Cuts at: 2787 3446 3772 2787 Size: 659 326 5625 Fragments arranged by size: 5625 659 326 EcoO109I rG'GnC_Cy Cuts at: 2497 2950 3262 3269 3553 3631 3715 3753 2497 Size: 453 312 7 284 78 84 38 5354 Fragments arranged by size: 5354 453 312 284 84 78 38 7 EcoRI G'AATT_C Cuts at: 3919 4297 3919 Size: 378 6232 FspI TGC'GCA Cuts at: 5116 5116 Size: 6610 HaeII r_GCGC'y Cuts at: 1678 2825 3326 3781 3879 5757 1678 Size: 1147 501 455 98 1878 2531 Fragments arranged by size: 2531 1878 1147 501 455 98 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2663 2695 2732 2764 3158 Size: 32 135 32 1091 32 37 32 394 Cuts at: 3158 3190 5251 5283 5325 5357 1373 Size: 32 2061 32 42 32 2626 Fragments arranged by size: 2626 2061 1091 394 135 42 37 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3524 3954 4200 669 Size: 988 281 1586 430 246 3079 Fragments arranged by size: 3079 1586 988 430 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6610 HpaI GTT'AAC Cuts at: 4200 4200 Size: 6610 KasI G'GCGC_C Cuts at: 3875 3875 Size: 6610 KpnI G_GTAC'C Cuts at: 3935 3935 Size: 6610 MfeI C'AATT_G Cuts at: 4209 4209 Size: 6610 MluI A'CGCG_T Cuts at: 6486 6486 Size: 6610 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2251 3156 5724 5908 1305 Size: 946 905 2568 184 2007 Fragments arranged by size: 2568 2007 946 905 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2579 2655 10 Size: 54 1425 343 747 76 3965 Fragments arranged by size: 3965 1425 747 343 76 54 MslI CAynn'nnrTG Cuts at: 518 1166 2207 3161 3278 4568 4927 5086 518 Size: 648 1041 954 117 1290 359 159 2042 Fragments arranged by size: 2042 1290 1041 954 648 359 159 117 MspA1I CmG'CkG Cuts at: 1606 2195 2434 2649 2935 2980 3301 3365 3383 Size: 589 239 215 286 45 321 64 18 Cuts at: 3383 3620 3702 4026 4703 5851 6096 1606 Size: 237 82 324 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 589 324 321 286 245 239 237 215 82 64 45 18 NaeI GCC'GGC Cuts at: 3855 3855 Size: 6610 NarI GG'CG_CC Cuts at: 3876 3876 Size: 6610 NcoI C'CATG_G Cuts at: 513 1065 2004 3162 3279 513 Size: 552 939 1158 117 3844 Fragments arranged by size: 3844 1158 939 552 117 NdeI CA'TA_TG Cuts at: 387 3666 387 Size: 3279 3331 NgoMIV G'CCGG_C Cuts at: 3853 3853 Size: 6610 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6610 NotI GC'GGCC_GC Cuts at: 4057 4057 Size: 6610 NspI r_CATG'y Cuts at: 1132 1229 2356 3129 3968 5513 1132 Size: 97 1127 773 839 1545 2229 Fragments arranged by size: 2229 1545 1127 839 773 97 PasI CC'CwG_GG Cuts at: 1348 2061 3825 1348 Size: 713 1764 4133 Fragments arranged by size: 4133 1764 713 PciI A'CATG_T Cuts at: 5509 5509 Size: 6610 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6610 PfoI T'CCnGG_A Cuts at: 2448 3075 3432 2448 Size: 627 357 5626 Fragments arranged by size: 5626 627 357 PmeI GTTT'AAAC Cuts at: 3914 3914 Size: 6610 PmlI CAC'GTG Cuts at: 3316 3316 Size: 6610 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4679 4711 6192 6224 4679 Size: 32 1481 32 5065 Fragments arranged by size: 5065 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2497 2950 3262 3553 3631 3753 2497 Size: 453 312 291 78 122 5354 Fragments arranged by size: 5354 453 312 291 122 78 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6610 PsiI TTA'TAA Cuts at: 4180 4180 Size: 6610 PspOMI G'GGCC_C Cuts at: 2801 2801 Size: 6610 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2450 2482 2450 Size: 32 6578 PstI C_TGCA'G Cuts at: 830 2235 2766 3603 3962 830 Size: 1405 531 837 359 3478 Fragments arranged by size: 3478 1405 837 531 359 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6610 PvuII CAG'CTG Cuts at: 2434 3301 3365 3620 2434 Size: 867 64 255 5424 Fragments arranged by size: 5424 867 255 64 SacI G_AGCT'C Cuts at: 3432 3432 Size: 6610 SalI G'TCGA_C Cuts at: 1936 3952 1936 Size: 2016 4594 SanDI GG'GwC_CC Cuts at: 2950 3631 3753 2950 Size: 681 122 5807 Fragments arranged by size: 5807 681 122 SbfI CC_TGCA'GG Cuts at: 2766 3962 2766 Size: 1196 5414 ScaI AGT'ACT Cuts at: 4858 6570 4858 Size: 1712 4898 SexAI A'CCwGG_T Cuts at: 2469 2469 Size: 6610 SfcI C'TryA_G Cuts at: 826 1045 2231 2762 3599 3958 5093 5774 5965 Size: 219 1186 531 837 359 1135 681 191 Cuts at: 5965 826 Size: 1471 Fragments arranged by size: 1471 1186 1135 837 681 531 359 219 191 SfoI GGC'GCC Cuts at: 3877 3877 Size: 6610 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6610 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6610 SmaI CCC'GGG Cuts at: 1872 3644 3937 1872 Size: 1772 293 4545 Fragments arranged by size: 4545 1772 293 SmlI C'TyrA_G Cuts at: 820 1017 1943 2216 2687 2964 3305 4714 5615 Size: 197 926 273 471 277 341 1409 901 Cuts at: 5615 5877 820 Size: 262 1553 Fragments arranged by size: 1553 1409 926 901 471 341 277 273 262 197 SnaBI TAC'GTA Cuts at: 493 4401 493 Size: 3908 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6610 SphI G_CATG'C Cuts at: 1132 3968 1132 Size: 2836 3774 SspI AAT'ATT Cuts at: 5 52 4534 5 Size: 47 4482 2081 Fragments arranged by size: 4482 2081 47 StuI AGG'CCT Cuts at: 1517 3762 1517 Size: 2245 4365 StyI C'CwwG_G Cuts at: 513 1065 2004 2078 3162 3279 3757 4049 513 Size: 552 939 74 1084 117 478 292 3074 Fragments arranged by size: 3074 1084 939 552 478 292 117 74 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2816 3292 4637 4654 4807 4992 5437 Size: 1255 1236 476 1345 17 153 185 445 Cuts at: 5437 325 Size: 1498 Fragments arranged by size: 1498 1345 1255 1236 476 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4856 6568 96 Size: 274 80 33 51 1443 2879 1712 138 Fragments arranged by size: 2879 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2405 2437 6570 6602 2405 Size: 32 4133 32 2413 Fragments arranged by size: 4133 2413 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3172 1316 Size: 1856 4754 XbaI T'CTAG_A Cuts at: 3946 3946 Size: 6610 XhoI C'TCGA_G Cuts at: 1943 2964 1943 Size: 1021 5589 XmaI C'CCGG_G Cuts at: 1870 3642 3935 1870 Size: 1772 293 4545 Fragments arranged by size: 4545 1772 293 XmnI GAAnn'nnTTC Cuts at: 4739 4739 Size: 6610 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4418 276 Size: 53 83 186 936 2884 2468 Fragments arranged by size: 2884 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PspXI RsrII SacII SapI SfiI SrfI SwaI XcmI