(Circular) (Six-base) MAPSORT of: pFN21AB4274.seq Check: 4689 from: 1 to: 6646 pFN21AB4274 6646 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4456 278 Size: 53 83 186 936 2920 2468 Fragments arranged by size: 2920 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2692 3989 1937 Size: 755 1297 4594 Fragments arranged by size: 4594 1297 755 Acc65I G'GTAC_C Cuts at: 3967 3967 Size: 6646 AclI AA'CG_TT Cuts at: 1563 4773 5146 1563 Size: 3210 373 3063 Fragments arranged by size: 3210 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2265 2562 3025 4709 6093 6361 1632 Size: 633 297 463 1684 1384 268 1917 Fragments arranged by size: 1917 1684 1384 633 463 297 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6449 AflIII A'CryG_T Cuts at: 1203 5545 6522 1203 Size: 4342 977 1327 Fragments arranged by size: 4342 1327 977 AgeI A'CCGG_T Cuts at: 5477 5477 Size: 6646 AhdI GACnn_n'nnGTC Cuts at: 5375 5375 Size: 6646 AleI CACnn'nnGTG Cuts at: 1166 3840 1166 Size: 2674 3972 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6646 AlwNI CAG_nnn'CTG Cuts at: 2071 4389 5961 2071 Size: 2318 1572 2756 Fragments arranged by size: 2756 2318 1572 ApaLI G'TGCA_C Cuts at: 4703 5859 4703 Size: 1156 5490 ApoI r'AATT_y Cuts at: 1493 2261 2380 2823 3579 3955 4181 4333 6442 Size: 768 119 443 756 376 226 152 2109 Cuts at: 6442 1493 Size: 1697 Fragments arranged by size: 2109 1697 768 756 443 376 226 152 119 AseI AT'TA_AT Cuts at: 160 1032 2444 5200 160 Size: 872 1412 2756 1606 Fragments arranged by size: 2756 1606 1412 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6646 AvaI C'yCGr_G Cuts at: 1870 1943 3971 1870 Size: 73 2028 4545 Fragments arranged by size: 4545 2028 73 BamHI G'GATC_C Cuts at: 3976 4343 3976 Size: 367 6279 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3967 5422 618 Size: 325 217 627 2180 1455 1842 Fragments arranged by size: 2180 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3965 1391 Size: 2574 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2093 2253 3140 3308 3392 3812 928 Size: 971 194 160 887 168 84 420 3762 Fragments arranged by size: 3762 971 887 420 194 168 160 84 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4837 4871 1903 Size: 34 2900 34 3678 Fragments arranged by size: 3678 2900 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4538 5748 4538 Size: 1210 5436 BclI T'GATC_A Cuts at: 1548 2896 2980 3484 1548 Size: 1348 84 504 4710 Fragments arranged by size: 4710 1348 504 84 BfrBI ATG'CAT Cuts at: 2870 2870 Size: 6646 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5257 136 Size: 107 122 71 631 605 3585 1525 Fragments arranged by size: 3585 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6641 6641 Size: 6646 BlpI GC'TnA_GC Cuts at: 4063 4063 Size: 6646 Bme1580I G_kGCm'C Cuts at: 1790 4707 5863 1790 Size: 2917 1156 2573 Fragments arranged by size: 2917 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6646 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4377 5335 442 Size: 416 53 83 399 24 2960 958 1753 Fragments arranged by size: 2960 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6646 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2604 5306 1239 Size: 1365 2702 2579 Fragments arranged by size: 2702 2579 1365 Bpu10I CC'TnA_GC Cuts at: 2010 2010 Size: 6646 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2156 2948 4771 5636 5934 2156 Size: 792 1823 865 298 2868 Fragments arranged by size: 2868 1823 865 792 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5309 882 Size: 617 3810 2219 Fragments arranged by size: 3810 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4437 493 Size: 711 3233 2702 Fragments arranged by size: 3233 2702 711 BsaBI GATnn'nnATC Cuts at: 6640 6640 Size: 6646 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4453 4835 5470 275 Size: 53 83 186 936 2920 382 635 1451 Fragments arranged by size: 2920 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5079 5477 5751 5898 6309 5079 Size: 398 274 147 411 5416 Fragments arranged by size: 5416 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2602 2632 2996 3026 1178 Size: 30 165 30 1199 30 364 30 4798 Fragments arranged by size: 4798 1199 364 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 2199 3353 1186 Size: 886 127 1154 4479 Fragments arranged by size: 4479 1154 886 127 BseYI C'CCAG_C Cuts at: 5849 6345 5849 Size: 496 6150 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6073 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4096 4857 5885 1540 Size: 275 186 2095 761 1028 2301 Fragments arranged by size: 2301 2095 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4707 4792 5863 4707 Size: 85 1071 5490 Fragments arranged by size: 5490 1071 85 BsmI GAATG_Cn' Cuts at: 3040 4157 4250 3040 Size: 1117 93 5436 Fragments arranged by size: 5436 1117 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3965 4707 4792 5863 1391 Size: 399 2175 742 85 1071 2174 Fragments arranged by size: 2175 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6309 6309 Size: 6646 BspHI T'CATG_A Cuts at: 3319 4535 6265 3319 Size: 1216 1730 3700 Fragments arranged by size: 3700 1730 1216 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2201 2228 4001 844 Size: 1357 27 1773 3489 Fragments arranged by size: 3489 1773 1357 27 BsrBI CCG'CTC Cuts at: 1394 1823 4533 1394 Size: 429 2710 3507 Fragments arranged by size: 3507 2710 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2776 2944 3032 5141 5315 65 Size: 1183 1528 168 88 2109 174 1396 Fragments arranged by size: 2109 1528 1396 1183 174 168 88 BsrFI r'CCGG_y Cuts at: 1700 1810 3671 5290 5477 1700 Size: 110 1861 1619 187 2869 Fragments arranged by size: 2869 1861 1619 187 110 BsrGI T'GTAC_A Cuts at: 96 3232 3652 3820 96 Size: 3136 420 168 2922 Fragments arranged by size: 3136 2922 420 168 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6646 BssSI C'ACGA_G Cuts at: 4706 5718 4706 Size: 1012 5634 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6646 BstBI TT'CG_AA Cuts at: 5450 5450 Size: 6646 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6646 BstYI r'GATC_y Cuts at: 1972 3976 4343 4728 4745 6197 6283 6295 6641 Size: 2004 367 385 17 1452 86 12 346 Cuts at: 6641 1972 Size: 1977 Fragments arranged by size: 2004 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2195 513 Size: 552 939 191 4964 Fragments arranged by size: 4964 939 552 191 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5907 Fragments arranged by size: 5907 683 56 BtsI GCAGTG_nn' Cuts at: 4162 4956 4976 4162 Size: 794 20 5832 Fragments arranged by size: 5832 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6646 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2758 2793 2926 2961 529 Size: 35 2194 35 133 35 4214 Fragments arranged by size: 4214 2194 133 35 35 35 DraI TTT'AAA Cuts at: 3950 3950 Size: 6646 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5653 809 Size: 507 4337 1802 Fragments arranged by size: 4337 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3673 4093 4982 Size: 54 1425 50 275 18 1843 420 889 Cuts at: 4982 6611 8 Size: 1629 43 Fragments arranged by size: 1843 1629 1425 889 420 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4093 1537 Size: 275 2281 4090 Fragments arranged by size: 4090 2281 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2184 2327 2731 3321 4576 1353 Size: 831 143 404 590 1255 3423 Fragments arranged by size: 3423 1255 831 590 404 143 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2948 3116 3368 3788 5233 5607 Size: 1064 138 1359 168 252 420 1445 374 Cuts at: 5607 5753 387 Size: 146 1280 Fragments arranged by size: 1445 1359 1280 1064 420 374 252 168 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2265 2562 2604 3025 4709 5306 6093 Size: 393 633 297 42 421 1684 597 787 Cuts at: 6093 6361 1239 Size: 268 1524 Fragments arranged by size: 1684 1524 787 633 597 421 393 297 268 42 EcoNI CCTnn'n_nnAGG Cuts at: 2700 3715 2700 Size: 1015 5631 EcoRI G'AATT_C Cuts at: 2380 2823 3579 3955 4333 2380 Size: 443 756 376 378 4693 Fragments arranged by size: 4693 756 443 378 376 FspI TGC'GCA Cuts at: 5152 5152 Size: 6646 HaeII r_GCGC'y Cuts at: 1678 5793 1678 Size: 4115 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2344 2376 2521 2553 2800 Size: 32 135 32 772 32 145 32 247 Cuts at: 2800 2832 5287 5319 5361 5393 1373 Size: 32 2455 32 42 32 2626 Fragments arranged by size: 2626 2455 772 247 145 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3990 4236 669 Size: 988 281 2052 246 3079 Fragments arranged by size: 3079 2052 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6646 HpaI GTT'AAC Cuts at: 4236 4236 Size: 6646 KpnI G_GTAC'C Cuts at: 3971 3971 Size: 6646 MfeI C'AATT_G Cuts at: 4245 4245 Size: 6646 MluI A'CGCG_T Cuts at: 6522 6522 Size: 6646 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2091 2157 5760 5944 1305 Size: 786 66 3603 184 2007 Fragments arranged by size: 3603 2007 786 184 66 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4824 Fragments arranged by size: 4824 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2135 2859 3100 3168 3520 3772 3840 Size: 648 969 724 241 68 352 252 68 Cuts at: 3840 3938 4604 4963 5122 518 Size: 98 666 359 159 2042 Fragments arranged by size: 2042 969 724 666 648 359 352 252 241 159 98 68 68 MspA1I CmG'CkG Cuts at: 1606 4062 4739 5887 6132 1606 Size: 2456 677 1148 245 2120 Fragments arranged by size: 2456 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2195 513 Size: 552 939 191 4964 Fragments arranged by size: 4964 939 552 191 NdeI CA'TA_TG Cuts at: 387 3097 3517 387 Size: 2710 420 3516 Fragments arranged by size: 3516 2710 420 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6646 NotI GC'GGCC_GC Cuts at: 4093 4093 Size: 6646 NsiI A_TGCA'T Cuts at: 2872 2872 Size: 6646 NspI r_CATG'y Cuts at: 1132 1229 2769 4004 5549 1132 Size: 97 1540 1235 1545 2229 Fragments arranged by size: 2229 1545 1540 1235 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6646 PciI A'CATG_T Cuts at: 5545 5545 Size: 6646 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6646 PmeI GTTT'AAAC Cuts at: 3950 3950 Size: 6646 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4715 4747 6228 6260 4715 Size: 32 1481 32 5101 Fragments arranged by size: 5101 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6646 PsiI TTA'TAA Cuts at: 3888 4216 3888 Size: 328 6318 PstI C_TGCA'G Cuts at: 830 2795 3998 830 Size: 1965 1203 3478 Fragments arranged by size: 3478 1965 1203 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6646 SalI G'TCGA_C Cuts at: 1936 3988 1936 Size: 2052 4594 SapI GCTCTTCn'nnn_ Cuts at: 2184 2731 3321 2184 Size: 547 590 5509 Fragments arranged by size: 5509 590 547 SbfI CC_TGCA'GG Cuts at: 3998 3998 Size: 6646 ScaI AGT'ACT Cuts at: 2518 4894 6606 2518 Size: 2376 1712 2558 Fragments arranged by size: 2558 2376 1712 SfcI C'TryA_G Cuts at: 826 1045 2791 3994 5129 5810 6001 826 Size: 219 1746 1203 1135 681 191 1471 Fragments arranged by size: 1746 1471 1203 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6646 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6646 SmaI CCC'GGG Cuts at: 1872 3973 1872 Size: 2101 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2171 2963 4750 5651 5913 820 Size: 197 926 228 792 1787 901 262 1553 Fragments arranged by size: 1787 1553 926 901 792 262 228 197 SnaBI TAC'GTA Cuts at: 493 4437 493 Size: 3944 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6646 SphI G_CATG'C Cuts at: 1132 4004 1132 Size: 2872 3774 SspI AAT'ATT Cuts at: 5 52 2749 4570 5 Size: 47 2697 1821 2081 Fragments arranged by size: 2697 2081 1821 47 StuI AGG'CCT Cuts at: 1517 2676 3458 1517 Size: 1159 782 4705 Fragments arranged by size: 4705 1159 782 StyI C'CwwG_G Cuts at: 513 1065 2004 2195 4085 513 Size: 552 939 191 1890 3074 Fragments arranged by size: 3074 1890 939 552 191 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4673 4690 4843 5028 5473 325 Size: 1255 3093 17 153 185 445 1498 Fragments arranged by size: 3093 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2516 3232 3652 Size: 274 80 33 51 1443 539 716 420 Cuts at: 3652 3820 4892 6604 96 Size: 168 1072 1712 138 Fragments arranged by size: 1712 1443 1072 716 539 420 274 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6606 6638 6606 Size: 32 6614 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6646 XbaI T'CTAG_A Cuts at: 2288 3982 2288 Size: 1694 4952 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6646 XmaI C'CCGG_G Cuts at: 1870 3971 1870 Size: 2101 4545 XmnI GAAnn'nnTTC Cuts at: 2657 2786 3038 3122 3290 4775 2657 Size: 129 252 84 168 1485 4528 Fragments arranged by size: 4528 1485 252 168 129 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4454 276 Size: 53 83 186 936 2920 2468 Fragments arranged by size: 2920 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI