(Circular) (Six-base) MAPSORT of: pFN21AB4270.seq Check: 4614 from: 1 to: 5962 pFN21AB4270 5962 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3772 278 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3305 1937 Size: 1368 4594 Acc65I G'GTAC_C Cuts at: 3283 3283 Size: 5962 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2228 2865 4025 5409 5677 1632 Size: 596 637 1160 1384 268 1917 Fragments arranged by size: 1917 1384 1160 637 596 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 2385 2933 4861 5838 1203 Size: 1182 548 1928 977 1327 Fragments arranged by size: 1928 1327 1182 977 548 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 4691 4691 Size: 5962 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5962 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5962 AlwNI CAG_nnn'CTG Cuts at: 3705 5277 3705 Size: 1572 4390 ApaI G_GGCC'C Cuts at: 2989 2989 Size: 5962 ApaLI G'TGCA_C Cuts at: 4019 5175 4019 Size: 1156 4806 ApoI r'AATT_y Cuts at: 1493 2456 3134 3271 3497 3649 5758 1493 Size: 963 678 137 226 152 2109 1697 Fragments arranged by size: 2109 1697 963 678 226 152 137 AseI AT'TA_AT Cuts at: 160 1032 4516 160 Size: 872 3484 1606 Fragments arranged by size: 3484 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 2676 3287 1870 Size: 73 733 611 4545 Fragments arranged by size: 4545 733 611 73 BamHI G'GATC_C Cuts at: 2829 3292 3659 2829 Size: 463 367 5132 Fragments arranged by size: 5132 463 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3283 4738 618 Size: 325 217 627 1496 1455 1842 Fragments arranged by size: 1842 1496 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2683 2989 3281 1391 Size: 1292 306 292 4072 Fragments arranged by size: 4072 1292 306 292 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2002 2962 928 Size: 971 103 960 3928 Fragments arranged by size: 3928 971 960 103 BbvCI CC'TCA_GC Cuts at: 2858 2858 Size: 5962 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4153 4187 1903 Size: 34 2216 34 3678 Fragments arranged by size: 3678 2216 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3207 3854 5064 3207 Size: 647 1210 4105 Fragments arranged by size: 4105 1210 647 BclI T'GATC_A Cuts at: 1548 1548 Size: 5962 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4573 136 Size: 107 122 71 631 605 2901 1525 Fragments arranged by size: 2901 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5957 5957 Size: 5962 BlpI GC'TnA_GC Cuts at: 3379 3379 Size: 5962 Bme1580I G_kGCm'C Cuts at: 1790 2205 2989 4023 5179 1790 Size: 415 784 1034 1156 2573 Fragments arranged by size: 2573 1156 1034 784 415 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5962 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2680 2885 3693 Size: 416 53 83 399 24 1263 205 808 Cuts at: 3693 4651 442 Size: 958 1753 Fragments arranged by size: 1753 1263 958 808 416 399 205 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3142 4622 1239 Size: 1903 1480 2579 Fragments arranged by size: 2579 1903 1480 Bpu10I CC'TnA_GC Cuts at: 2589 2858 2589 Size: 269 5693 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2845 3074 4087 4952 5250 2845 Size: 229 1013 865 298 3557 Fragments arranged by size: 3557 1013 865 298 229 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2885 2890 4625 882 Size: 617 1386 5 1735 2219 Fragments arranged by size: 2219 1735 1386 617 5 BsaAI yAC'GTr Cuts at: 493 1204 2936 3753 493 Size: 711 1732 817 2702 Fragments arranged by size: 2702 1732 817 711 BsaBI GATnn'nnATC Cuts at: 5956 5956 Size: 5962 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3769 4151 4786 275 Size: 53 83 186 936 2236 382 635 1451 Fragments arranged by size: 2236 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4395 4793 5067 5214 5625 4395 Size: 398 274 147 411 4732 Fragments arranged by size: 4732 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2083 2113 2143 2173 1178 Size: 30 165 30 680 30 30 30 4967 Fragments arranged by size: 4967 680 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5962 BseYI C'CCAG_C Cuts at: 2982 5165 5661 2982 Size: 2183 496 3283 Fragments arranged by size: 3283 2183 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5389 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3412 4173 5201 1540 Size: 275 186 1411 761 1028 2301 Fragments arranged by size: 2301 1411 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2683 4023 4108 5179 2683 Size: 1340 85 1071 3466 Fragments arranged by size: 3466 1340 1071 85 BsmI GAATG_Cn' Cuts at: 3473 3566 3473 Size: 93 5869 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2205 2683 2989 3281 4023 4108 5179 Size: 399 415 478 306 292 742 85 1071 Cuts at: 5179 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 478 415 399 306 292 85 BspEI T'CCGG_A Cuts at: 5625 5625 Size: 5962 BspHI T'CATG_A Cuts at: 2883 3851 5581 2883 Size: 968 1730 3264 Fragments arranged by size: 3264 1730 968 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2670 3317 844 Size: 1826 647 3489 Fragments arranged by size: 3489 1826 647 BsrBI CCG'CTC Cuts at: 1394 1823 3849 1394 Size: 429 2026 3507 Fragments arranged by size: 3507 2026 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2129 4457 4631 65 Size: 1183 881 2328 174 1396 Fragments arranged by size: 2328 1396 1183 881 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4606 4793 1700 Size: 110 2796 187 2869 Fragments arranged by size: 2869 2796 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5962 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5962 BssSI C'ACGA_G Cuts at: 4022 5034 4022 Size: 1012 4950 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5962 BstBI TT'CG_AA Cuts at: 4766 4766 Size: 5962 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5962 BstXI CCAn_nnnn'nTGG Cuts at: 2875 2875 Size: 5962 BstYI r'GATC_y Cuts at: 1972 2829 3292 3659 4044 4061 5513 5599 5611 Size: 857 463 367 385 17 1452 86 12 Cuts at: 5611 5957 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 857 463 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2154 513 Size: 552 939 150 4321 Fragments arranged by size: 4321 939 552 150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5223 Fragments arranged by size: 5223 683 56 BtsI GCAGTG_nn' Cuts at: 2025 2403 3478 4272 4292 2025 Size: 378 1075 794 20 3695 Fragments arranged by size: 3695 1075 794 378 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5962 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5927 DraI TTT'AAA Cuts at: 2929 3266 2929 Size: 337 5625 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3216 4969 809 Size: 507 1900 1753 1802 Fragments arranged by size: 1900 1802 1753 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3409 4298 5927 Size: 54 1425 50 275 18 1579 889 1629 Cuts at: 5927 8 Size: 43 Fragments arranged by size: 1629 1579 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3409 1537 Size: 275 1597 4090 Fragments arranged by size: 4090 1597 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2247 3892 1353 Size: 894 1645 3423 Fragments arranged by size: 3423 1645 894 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4549 4923 5069 387 Size: 1064 138 2960 374 146 1280 Fragments arranged by size: 2960 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2681 2681 Size: 5962 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2228 2865 3142 4025 4622 5409 5677 Size: 393 596 637 277 883 597 787 268 Cuts at: 5677 1239 Size: 1524 Fragments arranged by size: 1524 883 787 637 597 596 393 277 268 EcoNI CCTnn'n_nnAGG Cuts at: 2861 2861 Size: 5962 EcoO109I rG'GnC_Cy Cuts at: 2986 2986 Size: 5962 EcoRI G'AATT_C Cuts at: 3271 3649 3271 Size: 378 5584 FspI TGC'GCA Cuts at: 4468 4468 Size: 5962 HaeII r_GCGC'y Cuts at: 1678 5109 1678 Size: 3431 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2499 2531 4603 4635 4677 Size: 32 135 32 927 32 2072 32 42 Cuts at: 4677 4709 1373 Size: 32 2626 Fragments arranged by size: 2626 2072 927 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2025 3306 3552 669 Size: 988 281 87 1281 246 3079 Fragments arranged by size: 3079 1281 988 281 246 87 HindIII A'AGCT_T Cuts at: 748 748 Size: 5962 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KpnI G_GTAC'C Cuts at: 3287 3287 Size: 5962 MfeI C'AATT_G Cuts at: 3561 3561 Size: 5962 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2073 5076 5260 1305 Size: 768 3003 184 2007 Fragments arranged by size: 3003 2007 768 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4140 Fragments arranged by size: 4140 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2081 2918 3920 4279 4438 518 Size: 648 915 837 1002 359 159 2042 Fragments arranged by size: 2042 1002 915 837 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2198 3109 3378 4055 5203 5448 1606 Size: 592 911 269 677 1148 245 2120 Fragments arranged by size: 2120 1148 911 677 592 269 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2154 513 Size: 552 939 150 4321 Fragments arranged by size: 4321 939 552 150 NdeI CA'TA_TG Cuts at: 387 387 Size: 5962 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5962 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NspI r_CATG'y Cuts at: 1132 1229 2389 2410 3320 4865 1132 Size: 97 1160 21 910 1545 2229 Fragments arranged by size: 2229 1545 1160 910 97 21 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5962 PciI A'CATG_T Cuts at: 2385 4861 2385 Size: 2476 3486 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5962 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PmlI CAC'GTG Cuts at: 2936 2936 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2577 2609 4031 4063 5544 5576 2577 Size: 32 1422 32 1481 32 2963 Fragments arranged by size: 2963 1481 1422 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2124 1613 Size: 511 5451 PsiI TTA'TAA Cuts at: 3532 3532 Size: 5962 PspOMI G'GGCC_C Cuts at: 2985 2985 Size: 5962 PstI C_TGCA'G Cuts at: 830 2667 2895 3314 830 Size: 1837 228 419 3478 Fragments arranged by size: 3478 1837 419 228 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 PvuII CAG'CTG Cuts at: 2198 2198 Size: 5962 SacI G_AGCT'C Cuts at: 2683 2683 Size: 5962 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 4210 5922 4210 Size: 1712 4250 SfcI C'TryA_G Cuts at: 826 1045 2464 2663 2891 3310 4445 5126 5317 Size: 219 1419 199 228 419 1135 681 191 Cuts at: 5317 826 Size: 1471 Fragments arranged by size: 1471 1419 1135 681 419 228 219 199 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 3289 1872 Size: 1417 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2824 3089 4066 4967 5229 820 Size: 197 926 881 265 977 901 262 1553 Fragments arranged by size: 1553 977 926 901 881 265 262 197 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 3320 1132 Size: 2188 3774 SspI AAT'ATT Cuts at: 5 52 2089 3886 5 Size: 47 2037 1797 2081 Fragments arranged by size: 2081 2037 1797 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5962 StyI C'CwwG_G Cuts at: 513 1065 2004 2154 3401 513 Size: 552 939 150 1247 3074 Fragments arranged by size: 3074 1247 939 552 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3989 4006 4159 4344 4789 325 Size: 1255 2409 17 153 185 445 1498 Fragments arranged by size: 2409 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2192 2781 4208 Size: 274 80 33 51 1443 215 589 1427 Cuts at: 4208 5920 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1427 589 274 215 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2695 2727 5922 5954 2695 Size: 32 3195 32 2703 Fragments arranged by size: 3195 2703 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5962 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2693 2693 Size: 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 3287 1870 Size: 1417 4545 XmnI GAAnn'nnTTC Cuts at: 2459 4091 2459 Size: 1632 4330 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3770 276 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI