(Circular) (Six-base) MAPSORT of: pFN21AB4239.seq Check: 8439 from: 1 to: 8329 pFN21AB4239 8329 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4528 6139 278 Size: 53 83 186 936 2992 1611 2468 Fragments arranged by size: 2992 2468 1611 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2544 5672 1937 Size: 607 3128 4594 Fragments arranged by size: 4594 3128 607 Acc65I G'GTAC_C Cuts at: 5650 5650 Size: 8329 AclI AA'CG_TT Cuts at: 1563 6456 6829 1563 Size: 4893 373 3063 Fragments arranged by size: 4893 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2076 2817 3087 5512 6392 7776 8044 1632 Size: 444 741 270 2425 880 1384 268 1917 Fragments arranged by size: 2425 1917 1384 880 741 444 270 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8132 AflIII A'CryG_T Cuts at: 1203 7228 8205 1203 Size: 6025 977 1327 Fragments arranged by size: 6025 1327 977 AgeI A'CCGG_T Cuts at: 7160 7160 Size: 8329 AhdI GACnn_n'nnGTC Cuts at: 4396 7058 4396 Size: 2662 5667 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8329 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8329 AlwNI CAG_nnn'CTG Cuts at: 6072 7644 6072 Size: 1572 6757 ApaLI G'TGCA_C Cuts at: 6386 7542 6386 Size: 1156 7173 ApoI r'AATT_y Cuts at: 1493 2063 2375 2399 2686 3947 4664 4973 5376 Size: 570 312 24 287 1261 717 309 403 Cuts at: 5376 5638 5864 6016 8125 1493 Size: 262 226 152 2109 1697 Fragments arranged by size: 2109 1697 1261 717 570 403 312 309 287 262 226 152 24 AseI AT'TA_AT Cuts at: 160 1032 3687 6883 160 Size: 872 2655 3196 1606 Fragments arranged by size: 3196 2655 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8329 AvaI C'yCGr_G Cuts at: 1870 1943 5654 1870 Size: 73 3711 4545 Fragments arranged by size: 4545 3711 73 BamHI G'GATC_C Cuts at: 2283 3841 5659 6026 2283 Size: 1558 1818 367 4586 Fragments arranged by size: 4586 1818 1558 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4938 5552 5650 7105 618 Size: 325 217 627 3151 614 98 1455 1842 Fragments arranged by size: 3151 1842 1455 627 614 325 217 98 BanII G_rGCy'C Cuts at: 1391 4273 5648 1391 Size: 2882 1375 4072 Fragments arranged by size: 4072 2882 1375 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2739 3139 4428 928 Size: 971 840 400 1289 4829 Fragments arranged by size: 4829 1289 971 840 400 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3329 3363 3449 3483 6520 6554 1903 Size: 34 1392 34 86 34 3037 34 3678 Fragments arranged by size: 3678 3037 1392 86 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3590 6221 7431 3590 Size: 2631 1210 4488 Fragments arranged by size: 4488 2631 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8329 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4936 6940 136 Size: 107 122 71 631 605 3264 2004 1525 Fragments arranged by size: 3264 2004 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2563 8324 2563 Size: 5761 2568 BlpI GC'TnA_GC Cuts at: 5746 5746 Size: 8329 Bme1580I G_kGCm'C Cuts at: 1790 2301 6390 7546 1790 Size: 511 4089 1156 2573 Fragments arranged by size: 4089 2573 1156 511 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8329 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2357 3107 4268 Size: 416 53 83 399 24 940 750 1161 Cuts at: 4268 5096 6060 7018 442 Size: 828 964 958 1753 Fragments arranged by size: 1753 1161 964 958 940 828 750 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8329 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2920 5047 2920 Size: 2127 6202 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3422 3679 3927 4119 4425 4437 6989 1239 Size: 2183 257 248 192 306 12 2552 2579 Fragments arranged by size: 2579 2552 2183 306 257 248 192 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2804 3801 5002 5226 6454 7319 7617 2804 Size: 997 1201 224 1228 865 298 3516 Fragments arranged by size: 3516 1228 1201 997 865 298 224 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5508 5528 6992 882 Size: 617 4009 20 1464 2219 Fragments arranged by size: 4009 2219 1464 617 20 BsaAI yAC'GTr Cuts at: 493 1204 6120 493 Size: 711 4916 2702 Fragments arranged by size: 4916 2702 711 BsaBI GATnn'nnATC Cuts at: 4143 5361 8323 4143 Size: 1218 2962 4149 Fragments arranged by size: 4149 2962 1218 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4325 4525 6136 6518 Size: 53 83 186 936 2792 200 1611 382 Cuts at: 6518 7153 275 Size: 635 1451 Fragments arranged by size: 2792 1611 1451 936 635 382 200 186 83 53 BsaWI w'CCGG_w Cuts at: 3035 3838 6762 7160 7434 7581 7992 3035 Size: 803 2924 398 274 147 411 3372 Fragments arranged by size: 3372 2924 803 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4260 4290 5041 5071 1178 Size: 30 165 30 2857 30 751 30 4436 Fragments arranged by size: 4436 2857 751 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2450 2985 1186 Size: 1264 535 6530 Fragments arranged by size: 6530 1264 535 BseYI C'CCAG_C Cuts at: 3470 4931 7532 8028 3470 Size: 1461 2601 496 3771 Fragments arranged by size: 3771 2601 1461 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2917 1163 Size: 573 1181 6575 Fragments arranged by size: 6575 1181 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5779 6540 7568 1540 Size: 275 186 3778 761 1028 2301 Fragments arranged by size: 3778 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 6390 6475 7546 6390 Size: 85 1071 7173 Fragments arranged by size: 7173 1071 85 BsmI GAATG_Cn' Cuts at: 4960 5840 5933 4960 Size: 880 93 7356 Fragments arranged by size: 7356 880 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2301 4273 5648 6390 6475 7546 1391 Size: 399 511 1972 1375 742 85 1071 2174 Fragments arranged by size: 2174 1972 1375 1071 742 511 399 85 BspEI T'CCGG_A Cuts at: 3838 7992 3838 Size: 4154 4175 BspHI T'CATG_A Cuts at: 4114 6218 7948 4114 Size: 2104 1730 4495 Fragments arranged by size: 4495 2104 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5684 844 Size: 4840 3489 BsrBI CCG'CTC Cuts at: 1394 1823 6216 1394 Size: 429 4393 3507 Fragments arranged by size: 4393 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2608 3049 3334 3437 4310 5599 6824 Size: 1183 1360 441 285 103 873 1289 1225 Cuts at: 6824 6998 65 Size: 174 1396 Fragments arranged by size: 1396 1360 1289 1225 1183 873 441 285 174 103 BsrFI r'CCGG_y Cuts at: 1700 1810 6973 7160 1700 Size: 110 5163 187 2869 Fragments arranged by size: 5163 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2647 96 Size: 2551 5778 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8329 BssSI C'ACGA_G Cuts at: 6389 7401 6389 Size: 1012 7317 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8329 BstBI TT'CG_AA Cuts at: 7133 7133 Size: 8329 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8329 BstXI CCAn_nnnn'nTGG Cuts at: 4281 4281 Size: 8329 BstYI r'GATC_y Cuts at: 1972 2283 2443 2563 3841 4379 5564 5579 5659 Size: 311 160 120 1278 538 1185 15 80 Cuts at: 5659 6026 6411 6428 7880 7966 7978 8324 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1278 1185 538 385 367 346 311 160 120 86 80 17 15 12 BstZ17I GTA'TAC Cuts at: 2545 2545 Size: 8329 Bsu36I CC'TnA_GG Cuts at: 3357 5038 3357 Size: 1681 6648 BtgI C'CryG_G Cuts at: 513 1065 2004 3223 4215 4450 5518 513 Size: 552 939 1219 992 235 1068 3324 Fragments arranged by size: 3324 1219 1068 992 939 552 235 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7590 Fragments arranged by size: 7590 683 56 BtsI GCAGTG_nn' Cuts at: 2746 4794 5845 6639 6659 2746 Size: 2048 1051 794 20 4416 Fragments arranged by size: 4416 2048 1051 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8329 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2818 2853 529 Size: 35 2254 35 6005 Fragments arranged by size: 6005 2254 35 35 DraI TTT'AAA Cuts at: 5633 5633 Size: 8329 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5495 7336 809 Size: 507 4179 1841 1802 Fragments arranged by size: 4179 1841 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5776 6665 8294 Size: 54 1425 50 275 18 3946 889 1629 Cuts at: 8294 8 Size: 43 Fragments arranged by size: 3946 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5776 1537 Size: 275 3964 4090 Fragments arranged by size: 4090 3964 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2020 2982 3960 5283 5309 5531 6259 1353 Size: 667 962 978 1323 26 222 728 3423 Fragments arranged by size: 3423 1323 978 962 728 667 222 26 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6916 7290 7436 387 Size: 1064 138 5327 374 146 1280 Fragments arranged by size: 5327 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2076 2817 3087 3422 3679 3927 4119 Size: 393 444 741 270 335 257 248 192 Cuts at: 4119 4425 4437 5512 6392 6989 7776 8044 1239 Size: 306 12 1075 880 597 787 268 1524 Fragments arranged by size: 1524 1075 880 787 741 597 444 393 335 306 270 268 257 248 192 12 EcoNI CCTnn'n_nnAGG Cuts at: 2472 5436 2472 Size: 2964 5365 EcoRI G'AATT_C Cuts at: 5638 6016 5638 Size: 378 7951 EcoRV GAT'ATC Cuts at: 5070 5070 Size: 8329 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4737 5462 4737 Size: 725 7604 FspI TGC'GCA Cuts at: 6835 6835 Size: 8329 HaeII r_GCGC'y Cuts at: 1678 7476 1678 Size: 5798 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2019 2051 3458 3490 4382 Size: 32 135 32 447 32 1407 32 892 Cuts at: 4382 4414 5219 5251 5564 5596 6970 7002 7044 Size: 32 805 32 313 32 1374 32 42 Cuts at: 7044 7076 1373 Size: 32 2626 Fragments arranged by size: 2626 1407 1374 892 805 447 313 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2677 3118 4497 5673 5919 669 Size: 988 281 739 441 1379 1176 246 3079 Fragments arranged by size: 3079 1379 1176 988 739 441 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8329 HpaI GTT'AAC Cuts at: 2677 5919 2677 Size: 3242 5087 KpnI G_GTAC'C Cuts at: 5654 5654 Size: 8329 MfeI C'AATT_G Cuts at: 2076 2448 5928 2076 Size: 372 3480 4477 Fragments arranged by size: 4477 3480 372 MluI A'CGCG_T Cuts at: 8205 8205 Size: 8329 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2132 4551 7443 7627 1305 Size: 827 2419 2892 184 2007 Fragments arranged by size: 2892 2419 2007 827 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6507 Fragments arranged by size: 6507 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2206 3423 4067 6287 6646 6805 518 Size: 648 1040 1217 644 2220 359 159 2042 Fragments arranged by size: 2220 2042 1217 1040 648 644 359 159 MspA1I CmG'CkG Cuts at: 1606 2328 4935 5745 6422 7570 7815 1606 Size: 722 2607 810 677 1148 245 2120 Fragments arranged by size: 2607 2120 1148 810 722 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3223 4215 4450 5518 513 Size: 552 939 1219 992 235 1068 3324 Fragments arranged by size: 3324 1219 1068 992 939 552 235 NdeI CA'TA_TG Cuts at: 387 3594 387 Size: 3207 5122 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8329 NotI GC'GGCC_GC Cuts at: 5776 5776 Size: 8329 NspI r_CATG'y Cuts at: 1132 1229 5687 7232 1132 Size: 97 4458 1545 2229 Fragments arranged by size: 4458 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3153 1348 Size: 1805 6524 PciI A'CATG_T Cuts at: 7228 7228 Size: 8329 PflMI CCAn_nnn'nTGG Cuts at: 1108 3134 4456 1108 Size: 2026 1322 4981 Fragments arranged by size: 4981 2026 1322 PfoI T'CCnGG_A Cuts at: 3656 3656 Size: 8329 PmeI GTTT'AAAC Cuts at: 5633 5633 Size: 8329 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2947 2979 6398 6430 7911 7943 2947 Size: 32 3419 32 1481 32 3333 Fragments arranged by size: 3419 3333 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3995 5520 1613 Size: 2382 1525 4422 Fragments arranged by size: 4422 2382 1525 PsiI TTA'TAA Cuts at: 4193 4466 5899 4193 Size: 273 1433 6623 Fragments arranged by size: 6623 1433 273 PstI C_TGCA'G Cuts at: 830 3517 5681 830 Size: 2687 2164 3478 Fragments arranged by size: 3478 2687 2164 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8329 PvuII CAG'CTG Cuts at: 4935 4935 Size: 8329 SalI G'TCGA_C Cuts at: 1936 5671 1936 Size: 3735 4594 SapI GCTCTTCn'nnn_ Cuts at: 2982 3960 5283 2982 Size: 978 1323 6028 Fragments arranged by size: 6028 1323 978 SbfI CC_TGCA'GG Cuts at: 5681 5681 Size: 8329 ScaI AGT'ACT Cuts at: 5336 6577 8289 5336 Size: 1241 1712 5376 Fragments arranged by size: 5376 1712 1241 SexAI A'CCwGG_T Cuts at: 4490 4490 Size: 8329 SfcI C'TryA_G Cuts at: 826 1045 3306 3501 3513 5604 5677 6812 7493 Size: 219 2261 195 12 2091 73 1135 681 Cuts at: 7493 7684 826 Size: 191 1471 Fragments arranged by size: 2261 2091 1471 1135 681 219 195 191 73 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8329 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8329 SmaI CCC'GGG Cuts at: 1872 5656 1872 Size: 3784 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2783 3816 4981 5241 6433 7334 Size: 197 926 840 1033 1165 260 1192 901 Cuts at: 7334 7596 820 Size: 262 1553 Fragments arranged by size: 1553 1192 1165 1033 926 901 840 262 260 197 SnaBI TAC'GTA Cuts at: 493 6120 493 Size: 5627 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8329 SphI G_CATG'C Cuts at: 1132 5687 1132 Size: 4555 3774 SspI AAT'ATT Cuts at: 5 52 2509 3250 3928 6253 5 Size: 47 2457 741 678 2325 2081 Fragments arranged by size: 2457 2325 2081 741 678 47 StuI AGG'CCT Cuts at: 1517 2478 4236 1517 Size: 961 1758 5610 Fragments arranged by size: 5610 1758 961 StyI C'CwwG_G Cuts at: 513 1065 2004 3223 3760 4000 4179 4215 4450 Size: 552 939 1219 537 240 179 36 235 Cuts at: 4450 5518 5768 513 Size: 1068 250 3074 Fragments arranged by size: 3074 1219 1068 939 552 537 250 240 235 179 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5115 5439 6356 6373 6526 6711 7156 Size: 1255 3535 324 917 17 153 185 445 Cuts at: 7156 325 Size: 1498 Fragments arranged by size: 3535 1498 1255 917 445 324 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2647 4242 4472 Size: 274 80 33 51 1443 670 1595 230 Cuts at: 4472 4516 4968 5334 6575 8287 96 Size: 44 452 366 1241 1712 138 Fragments arranged by size: 1712 1595 1443 1241 670 452 366 274 230 138 80 51 44 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4260 4292 5311 5343 8289 8321 4260 Size: 32 1019 32 2946 32 4268 Fragments arranged by size: 4268 2946 1019 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8329 XbaI T'CTAG_A Cuts at: 3793 5665 3793 Size: 1872 6457 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2205 2820 2947 2205 Size: 615 127 7587 Fragments arranged by size: 7587 615 127 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8329 XmaI C'CCGG_G Cuts at: 1870 5654 1870 Size: 3784 4545 XmnI GAAnn'nnTTC Cuts at: 3855 6458 3855 Size: 2603 5726 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4526 6137 276 Size: 53 83 186 936 2992 1611 2468 Fragments arranged by size: 2992 2468 1611 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI