Restriction map for
pFN21AB4237
(Linear) (Six-base) MAPSORT of: KIBB4237.seq Check: 1565 from: 1 to: 2451
KIBB4237.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 345 427 2451
Size: 345 82 2024
Fragments arranged by size:
2024 345 82
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 628 913 1421 2451
Size: 411 217 285 508 1030
Fragments arranged by size:
1030 508 411 285 217
AflIII A'CryG_T
Cuts at: 0 569 956 2451
Size: 569 387 1495
Fragments arranged by size:
1495 569 387
AleI CACnn'nnGTG
Cuts at: 0 1381 2451
Size: 1381 1070
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2053 2085 2451
Size: 2053 32 366
Fragments arranged by size:
2053 366 32
AlwNI CAG_nnn'CTG
Cuts at: 0 622 2451
Size: 622 1829
ApaI G_GGCC'C
Cuts at: 0 698 1524 1903 2451
Size: 698 826 379 548
Fragments arranged by size:
826 698 548 379
ApaLI G'TGCA_C
Cuts at: 0 324 2451
Size: 324 2127
ApoI r'AATT_y
Cuts at: 0 1868 1939 1951 2326 2451
Size: 1868 71 12 375 125
Fragments arranged by size:
1868 375 125 71 12
AseI AT'TA_AT
Cuts at: 0 1607 2451
Size: 1607 844
AvaI C'yCGr_G
Cuts at: 0 73 270 661 1039 1544 1779 2033 2451
Size: 73 197 391 378 505 235 254 418
Fragments arranged by size:
505 418 391 378 254 235 197 73
BamHI G'GATC_C
Cuts at: 0 1957 2451
Size: 1957 494
BanI G'GyrC_C
Cuts at: 0 68 136 252 2451
Size: 68 68 116 2199
Fragments arranged by size:
2199 116 68 68
BanII G_rGCy'C
Cuts at: 0 698 1471 1524 1574 1719 1903 2451
Size: 698 773 53 50 145 184 548
Fragments arranged by size:
773 698 548 184 145 53 50
BbeI G_GCGC'C
Cuts at: 0 72 140 2451
Size: 72 68 2311
Fragments arranged by size:
2311 72 68
BbvCI CC'TCA_GC
Cuts at: 0 1127 2451
Size: 1127 1324
BclI T'GATC_A
Cuts at: 0 1889 2451
Size: 1889 562
BglII A'GATC_T
Cuts at: 0 2294 2451
Size: 2294 157
BlpI GC'TnA_GC
Cuts at: 0 1573 2451
Size: 1573 878
Bme1580I G_kGCm'C
Cuts at: 0 31 328 698 1524 1903 2420 2451
Size: 31 297 370 826 379 517 31
Fragments arranged by size:
826 517 379 370 297 31 31
BmgBI CAC'GTC
Cuts at: 0 570 1990 2451
Size: 570 1420 461
Fragments arranged by size:
1420 570 461
BmrI ACTGGGnnnn_n'
Cuts at: 0 1383 1679 2451
Size: 1383 296 772
Fragments arranged by size:
1383 772 296
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2338 2451
Size: 2338 113
Bpu10I CC'TnA_GC
Cuts at: 0 260 1127 2451
Size: 260 867 1324
Fragments arranged by size:
1324 867 260
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 2451
Size: 421 2030
BsaI GGTCTCn'nnnn_
Cuts at: 0 658 1280 2451
Size: 658 622 1171
Fragments arranged by size:
1171 658 622
BsaBI GATnn'nnATC
Cuts at: 0 1535 2451
Size: 1535 916
BsaHI Gr'CG_yC
Cuts at: 0 69 137 810 2451
Size: 69 68 673 1641
Fragments arranged by size:
1641 673 69 68
BsaWI w'CCGG_w
Cuts at: 0 1289 2451
Size: 1289 1162
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1159 1189 1918 1948 2451
Size: 1159 30 729 30 503
Fragments arranged by size:
1159 729 503 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1064 1067 1144 1220 2451
Size: 1064 3 77 76 1231
Fragments arranged by size:
1231 1064 77 76 3
BseYI C'CCAG_C
Cuts at: 0 307 528 721 1254 2451
Size: 307 221 193 533 1197
Fragments arranged by size:
1197 533 307 221 193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1150 2451
Size: 1150 1301
BsiEI CG_ry'CG
Cuts at: 0 237 2451
Size: 237 2214
BsiHKAI G_wGCw'C
Cuts at: 0 283 328 1471 1719 2451
Size: 283 45 1143 248 732
Fragments arranged by size:
1143 732 283 248 45
BsmI GAATG_Cn'
Cuts at: 0 1329 2451
Size: 1329 1122
Bsp1286I G_dGCh'C
Cuts at: 0 31 283 328 698 1471 1524 1574 1719
Size: 31 252 45 370 773 53 50 145
Cuts at: 1719 1903 2420 2451
Size: 184 517 31
Fragments arranged by size:
773 517 370 252 184 145 53 50
45 31 31
BspHI T'CATG_A
Cuts at: 0 1332 2451
Size: 1332 1119
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 345 427 692 2451
Size: 345 82 265 1759
Fragments arranged by size:
1759 345 265 82
BsrDI GCAATG_nn'
Cuts at: 0 716 2181 2213 2451
Size: 716 1465 32 238
Fragments arranged by size:
1465 716 238 32
BsrFI r'CCGG_y
Cuts at: 0 232 249 827 1363 2451
Size: 232 17 578 536 1088
Fragments arranged by size:
1088 578 536 232 17
BsrGI T'GTAC_A
Cuts at: 0 2352 2451
Size: 2352 99
BssSI C'ACGA_G
Cuts at: 0 871 2451
Size: 871 1580
BstAPI GCAn_nnn'nTGC
Cuts at: 0 174 2153 2451
Size: 174 1979 298
Fragments arranged by size:
1979 298 174
BstEII G'GTnAC_C
Cuts at: 0 45 1644 2451
Size: 45 1599 807
Fragments arranged by size:
1599 807 45
BstXI CCAn_nnnn'nTGG
Cuts at: 0 705 1185 2316 2451
Size: 705 480 1131 135
Fragments arranged by size:
1131 705 480 135
BstYI r'GATC_y
Cuts at: 0 1292 1957 2294 2451
Size: 1292 665 337 157
Fragments arranged by size:
1292 665 337 157
Bsu36I CC'TnA_GG
Cuts at: 0 1079 2451
Size: 1079 1372
BtgI C'CryG_G
Cuts at: 0 515 2451
Size: 515 1936
BtsI GCAGTG_nn'
Cuts at: 0 15 372 2151 2451
Size: 15 357 1779 300
Fragments arranged by size:
1779 357 300 15
DraIII CAC_nnn'GTG
Cuts at: 0 2025 2451
Size: 2025 426
DrdI GACnn_nn'nnGTC
Cuts at: 0 100 2451
Size: 100 2351
EaeI y'GGCC_r
Cuts at: 0 56 234 316 926 1365 2451
Size: 56 178 82 610 439 1086
Fragments arranged by size:
1086 610 439 178 82 56
EagI C'GGCC_G
Cuts at: 0 234 2451
Size: 234 2217
EarI CTCTTCn'nnn_
Cuts at: 0 1687 2366 2451
Size: 1687 679 85
Fragments arranged by size:
1687 679 85
EcoICRI GAG'CTC
Cuts at: 0 1469 1717 2451
Size: 1469 248 734
Fragments arranged by size:
1469 734 248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 628 913 1421 2338 2451
Size: 411 217 285 508 917 113
Fragments arranged by size:
917 508 411 285 217 113
EcoO109I rG'GnC_Cy
Cuts at: 0 619 694 1481 1505 1520 1521 2430 2451
Size: 619 75 787 24 15 1 909 21
Fragments arranged by size:
909 787 619 75 24 21 15 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1524 2221 2451
Size: 1524 697 230
Fragments arranged by size:
1524 697 230
FseI GG_CCGG'CC
Cuts at: 0 1367 2451
Size: 1367 1084
FspI TGC'GCA
Cuts at: 0 1279 2451
Size: 1279 1172
HaeII r_GCGC'y
Cuts at: 0 72 140 607 2451
Size: 72 68 467 1844
Fragments arranged by size:
1844 467 72 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1277 1309 1918 1950 2395 2427 2451
Size: 1277 32 609 32 445 32 24
Fragments arranged by size:
1277 609 445 32 32 32 24
HincII GTy'rAC
Cuts at: 0 681 2451
Size: 681 1770
KasI G'GCGC_C
Cuts at: 0 68 136 2451
Size: 68 68 2315
Fragments arranged by size:
2315 68 68
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 2451
Size: 554 1897
MscI TGG'CCA
Cuts at: 0 318 928 2451
Size: 318 610 1523
Fragments arranged by size:
1523 610 318
MslI CAynn'nnrTG
Cuts at: 0 1381 2451
Size: 1381 1070
MspA1I CmG'CkG
Cuts at: 0 302 311 511 1258 1348 1553 2451
Size: 302 9 200 747 90 205 898
Fragments arranged by size:
898 747 302 205 200 90 9
NaeI GCC'GGC
Cuts at: 0 1365 2451
Size: 1365 1086
NarI GG'CG_CC
Cuts at: 0 69 137 2451
Size: 69 68 2314
Fragments arranged by size:
2314 69 68
NcoI C'CATG_G
Cuts at: 0 515 2451
Size: 515 1936
NdeI CA'TA_TG
Cuts at: 0 1845 2277 2451
Size: 1845 432 174
Fragments arranged by size:
1845 432 174
NgoMIV G'CCGG_C
Cuts at: 0 1363 2451
Size: 1363 1088
NspI r_CATG'y
Cuts at: 0 378 960 1122 1603 2451
Size: 378 582 162 481 848
Fragments arranged by size:
848 582 481 378 162
PciI A'CATG_T
Cuts at: 0 956 2451
Size: 956 1495
PflMI CCAn_nnn'nTGG
Cuts at: 0 314 2025 2451
Size: 314 1711 426
Fragments arranged by size:
1711 426 314
PpuMI rG'GwC_Cy
Cuts at: 0 1481 1505 2430 2451
Size: 1481 24 925 21
Fragments arranged by size:
1481 925 24 21
PsiI TTA'TAA
Cuts at: 0 2381 2451
Size: 2381 70
PspOMI G'GGCC_C
Cuts at: 0 694 1520 1899 2451
Size: 694 826 379 552
Fragments arranged by size:
826 694 552 379
PspXI vC'TCGA_Gb
Cuts at: 0 73 2451
Size: 73 2378
PstI C_TGCA'G
Cuts at: 0 860 1347 2195 2451
Size: 860 487 848 256
Fragments arranged by size:
860 848 487 256
PvuII CAG'CTG
Cuts at: 0 311 511 1258 1348 1553 2451
Size: 311 200 747 90 205 898
Fragments arranged by size:
898 747 311 205 200 90
SacI G_AGCT'C
Cuts at: 0 1471 1719 2451
Size: 1471 248 732
Fragments arranged by size:
1471 732 248
SanDI GG'GwC_CC
Cuts at: 0 1481 2430 2451
Size: 1481 949 21
Fragments arranged by size:
1481 949 21
ScaI AGT'ACT
Cuts at: 0 2338 2451
Size: 2338 113
SfcI C'TryA_G
Cuts at: 0 159 814 856 1343 2191 2451
Size: 159 655 42 487 848 260
Fragments arranged by size:
848 655 487 260 159 42
SfoI GGC'GCC
Cuts at: 0 70 138 2451
Size: 70 68 2313
Fragments arranged by size:
2313 70 68
SmaI CCC'GGG
Cuts at: 0 272 1781 2451
Size: 272 1509 670
Fragments arranged by size:
1509 670 272
SmlI C'TyrA_G
Cuts at: 0 73 400 2451
Size: 73 327 2051
Fragments arranged by size:
2051 327 73
StuI AGG'CCT
Cuts at: 0 704 1184 1884 2451
Size: 704 480 700 567
Fragments arranged by size:
704 700 567 480
StyI C'CwwG_G
Cuts at: 0 212 515 995 2451
Size: 212 303 480 1456
Fragments arranged by size:
1456 480 303 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1928 2451
Size: 1928 523
TatI w'GTAC_w
Cuts at: 0 1391 2336 2352 2451
Size: 1391 945 16 99
Fragments arranged by size:
1391 945 99 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1159 1191 1949 1981 2451
Size: 1159 32 758 32 470
Fragments arranged by size:
1159 758 470 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1915 2451
Size: 1915 536
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1119 2451
Size: 1119 1332
XhoI C'TCGA_G
Cuts at: 0 73 2451
Size: 73 2378
XmaI C'CCGG_G
Cuts at: 0 270 1779 2451
Size: 270 1509 672
Fragments arranged by size:
1509 672 270
Enzymes that do cut:
AarI AcuI AflIII AleI AloI AlwNI ApaI ApaLI
ApoI AseI AvaI BamHI BanI BanII BbeI BbvCI
BclI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtsI DraIII DrdI EaeI EagI EarI EcoICRI
Eco57MI EcoO109I FalI FseI FspI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PflMI PpuMI PsiI PspOMI
PspXI PstI PvuII SacI SanDI ScaI SfcI SfoI
SmaI SmlI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AscI AsiSI AvrII BaeI BbsI BcgI BciVI
BfrBI BglI BmtI BplI BsaAI BsiWI BsmBI BspEI
BsrBI BssHII BstBI BstZ17I BtgZI ClaI CspCI DraI
EciI EcoNI EcoRI EcoRV FspAI HindIII HpaI KpnI
MfeI MluI NheI NotI NruI NsiI PacI PasI
PfoI PmeI PmlI PpiI PshAI PsrI PvuI RsrII
SacII SalI SapI SbfI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI XbaI XmnI
ZraI