Restriction map for
pFN21AB4221
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII AcsI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
BamHI BcgI BciVI BclI BfrI
BglII BsaBI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
FbaI FseI HindIII HpaI KspI
MamI MfeI MflI MluI MseI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I Tsp509I TthHB8I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 172 GTCTAC GT^[AC][GT]AC
AccII 1190 CGCG CG^CG
AclI 274 AACGTT AA^CGTT
AcyI 211 GGCGCC G[GA]^CG[CT]C
AlwI 835 GATCC GGATC(4/5)
AocI 1177 CCTGAGG CC^T[ACGT]AGG
AspI 261 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 374 TGCGCA TGC^GCA
AvrII 1678 CCTAGG C^CTAGG
BbeI 211 GGCGCC GGCGC^C
BfaI 1679 CTAG C^TAG
BlnI 1678 CCTAGG C^CTAGG
BlpI 790 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 790 GCTGAGC GC^T[ACGT]AGC
BsaHI 211 GGCGCC G[GA]^CG[CT]C
BsaI 259 GAGACC GGTCTC(1/5)
BsgI 97 CTGCAC GTGCAG(16/14)
BsmAI 259 GAGAC GTCTC(1/5)
BsrBI 469 GAGCGG CCGCTC(-3/-3)
BsrFI 603 ACCGGC [GA]^CCGG[CT]
BstUI 1190 CGCG CG^CG
Bsu36I 1177 CCTGAGG CC^T[ACGT]AGG
CelII 790 GCTGAGC GC^T[ACGT]AGC
Cfr10I 603 ACCGGC [GA]^CCGG[CT]
Eco81I 1177 CCTGAGG CC^T[ACGT]AGG
EcoRV 481 GATATC GAT^ATC
EcoT22I 16 ATGCAT ATGCA^T
FspI 374 TGCGCA TGC^GCA
HaeII 211 GGCGCC [GA]GCGC^[CT]
HgaI 170 GCGTC GACGC(5/10)
Hin1I 211 GGCGCC G[GA]^CG[CT]C
HincII 1375 GTCAAC GT[CT]^[GA]AC
HindII 1375 GTCAAC GT[CT]^[GA]AC
KasI 211 GGCGCC G^GCGCC
MaeI 1679 CTAG C^TAG
MaeIII 992 GTGAC ^GT[ACGT]AC
MvnI 1190 CGCG CG^CG
NarI 211 GGCGCC GG^CGCC
NcoI 1184 CCATGG C^CATGG
NsiI 16 ATGCAT ATGCA^T
NspI 656 ACATGC [GA]CATG^[CT]
PflFI 261 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 733 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 274 AACGTT AA^CGTT
PstI 121 CTGCAG CTGCA^G
SfcI 121 CTGCAG C^T[GA][CT]AG
SfoI 211 GGCGCC GGC^GCC
Tsp45I 992 GTGAC ^GT[GC]AC
Tth111I 261 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 733 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 112 GGTACC G^GTACC
807 GGTACC
AccIII 872 TCCGGA T^CCGGA
1460 TCCGGA
ApaI 665 GGGCCC GGGCC^C
687 GGGCCC
Asp718I 112 GGTACC G^GTACC
807 GGTACC
BbrPI 318 CACGTG CAC^GTG
1621 CACGTG
BbsI 514 GAAGAC GAAGAC(2/6)
1369 GTCTTC
BbvCI 253 GCTGAGG CCTCAGC(-5/-2)
751 GCTGAGG
BglI 570 GCCCGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
961 GCCATCATGGC
BpuAI 514 GAAGAC GAAGAC(2/6)
1369 GTCTTC
BsaAI 318 CACGTG [CT]AC^GT[GA]
1621 CACGTG
BsaWI 872 TCCGGA [AT]^CCGG[AT]
1460 TCCGGA
BseAI 872 TCCGGA T^CCGGA
1460 TCCGGA
BspEI 872 TCCGGA T^CCGGA
1460 TCCGGA
EarI 10 GAAGAG CTCTTC(1/4)
1090 GAAGAG
HphI 240 GGTGA GGTGA(8/7)
1246 TCACC
KpnI 112 GGTACC GGTAC^C
807 GGTACC
Ksp632I 10 GAAGAG CTCTTC(1/4)
1090 GAAGAG
MroI 872 TCCGGA T^CCGGA
1460 TCCGGA
MslI 526 CACGACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1617 CACCCACGTG
MspA1I 118 CAGCTG C[AC]G^C[GT]G
124 CAGCTG
PmaCI 318 CACGTG CAC^GTG
1621 CACGTG
PmlI 318 CACGTG CAC^GTG
1621 CACGTG
PvuII 118 CAGCTG CAG^CTG
124 CAGCTG
SmaI 662 CCCGGG CCC^GGG
1427 CCCGGG
SmlI 580 CTTGAG C^T[CT][GA]AG
1000 CTCAAG
XmaCI 662 CCCGGG C^CCGGG
1427 CCCGGG
XmaI 662 CCCGGG C^CCGGG
1427 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeII MboI
MboII MluNI MnlI MscI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TseI
TspRI