(Circular) (Six-base) MAPSORT of: pFN21AB4221.seq Check: 4639 from: 1 to: 6388 pFN21AB4221 6388 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4198 278 Size: 53 83 186 936 2662 2468 Fragments arranged by size: 2662 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2178 3731 1937 Size: 241 1553 4594 Fragments arranged by size: 4594 1553 241 Acc65I G'GTAC_C Cuts at: 2117 2812 3709 2117 Size: 695 897 4796 Fragments arranged by size: 4796 897 695 AclI AA'CG_TT Cuts at: 1563 2280 4515 4888 1563 Size: 717 2235 373 3063 Fragments arranged by size: 3063 2235 717 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2034 3104 3114 3188 4451 5835 6103 1632 Size: 402 1070 10 74 1263 1384 268 1917 Fragments arranged by size: 1917 1384 1263 1070 402 268 74 10 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6191 AflIII A'CryG_T Cuts at: 1203 5287 6264 1203 Size: 4084 977 1327 Fragments arranged by size: 4084 1327 977 AgeI A'CCGG_T Cuts at: 5219 5219 Size: 6388 AhdI GACnn_n'nnGTC Cuts at: 5117 5117 Size: 6388 AleI CACnn'nnGTG Cuts at: 1166 3626 1166 Size: 2460 3928 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6388 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3398 3430 3545 3577 3398 Size: 32 115 32 6209 Fragments arranged by size: 6209 115 32 32 AlwNI CAG_nnn'CTG Cuts at: 2062 2134 2994 3182 3649 4131 5703 2062 Size: 72 860 188 467 482 1572 2747 Fragments arranged by size: 2747 1572 860 482 467 188 72 ApaI G_GGCC'C Cuts at: 2674 2696 2674 Size: 22 6366 ApaLI G'TGCA_C Cuts at: 4445 5601 4445 Size: 1156 5232 ApoI r'AATT_y Cuts at: 1493 3697 3923 4075 6184 1493 Size: 2204 226 152 2109 1697 Fragments arranged by size: 2204 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4942 160 Size: 872 3910 1606 Fragments arranged by size: 3910 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6388 AvaI C'yCGr_G Cuts at: 1870 1943 2667 2785 3027 3432 3713 1870 Size: 73 724 118 242 405 281 4545 Fragments arranged by size: 4545 724 405 281 242 118 73 AvrII C'CTAG_G Cuts at: 3683 3683 Size: 6388 BamHI G'GATC_C Cuts at: 3718 4085 3718 Size: 367 6021 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2051 2117 2216 2812 3355 Size: 325 217 627 264 66 99 596 543 Cuts at: 3355 3660 3709 5164 618 Size: 305 49 1455 1842 Fragments arranged by size: 1842 1455 627 596 543 325 305 264 217 99 66 49 BanII G_rGCy'C Cuts at: 1391 2577 2674 2696 2925 3051 3444 3707 1391 Size: 1186 97 22 229 126 393 263 4072 Fragments arranged by size: 4072 1186 393 263 229 126 97 22 BbeI G_GCGC'C Cuts at: 2220 2220 Size: 6388 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2526 3367 928 Size: 971 627 841 3949 Fragments arranged by size: 3949 971 841 627 BbvCI CC'TCA_GC Cuts at: 2259 2757 2259 Size: 498 5890 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4579 4613 1903 Size: 34 2642 34 3678 Fragments arranged by size: 3678 2642 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4280 5490 4280 Size: 1210 5178 BclI T'GATC_A Cuts at: 1548 1548 Size: 6388 BfrBI ATG'CAT Cuts at: 2023 2023 Size: 6388 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2581 2972 Size: 107 122 71 631 605 337 572 391 Cuts at: 2972 4999 136 Size: 2027 1525 Fragments arranged by size: 2027 1525 631 605 572 391 337 122 107 71 BglII A'GATC_T Cuts at: 6383 6383 Size: 6388 BlpI GC'TnA_GC Cuts at: 2796 3805 2796 Size: 1009 5379 Bme1580I G_kGCm'C Cuts at: 1790 2056 2634 2674 2696 2786 3141 4449 5605 Size: 266 578 40 22 90 355 1308 1156 Cuts at: 5605 1790 Size: 2573 Fragments arranged by size: 2573 1308 1156 578 355 266 90 40 22 BmgBI CAC'GTC Cuts at: 1318 2271 1318 Size: 953 5435 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2621 3687 4119 Size: 416 53 83 399 24 1204 1066 432 Cuts at: 4119 5077 442 Size: 958 1753 Fragments arranged by size: 1753 1204 1066 958 432 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6388 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2563 2581 3571 2563 Size: 18 990 5380 Fragments arranged by size: 5380 990 18 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2422 2576 2971 3227 3590 5048 1239 Size: 1183 154 395 256 363 1458 2579 Fragments arranged by size: 2579 1458 1183 395 363 256 154 Bpu10I CC'TnA_GC Cuts at: 2259 2724 2757 2259 Size: 465 33 5890 Fragments arranged by size: 5890 465 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2606 2990 4513 5378 5676 2606 Size: 384 1523 865 298 3318 Fragments arranged by size: 3318 1523 865 384 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2258 5051 882 Size: 617 759 2793 2219 Fragments arranged by size: 2793 2219 759 617 BsaAI yAC'GTr Cuts at: 493 1204 2325 3628 4179 493 Size: 711 1121 1303 551 2702 Fragments arranged by size: 2702 1303 1121 711 551 BsaBI GATnn'nnATC Cuts at: 6382 6382 Size: 6388 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2217 4195 4577 5212 Size: 53 83 186 936 684 1978 382 635 Cuts at: 5212 275 Size: 1451 Fragments arranged by size: 1978 1451 936 684 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2877 3465 4821 5219 5493 5640 6051 2877 Size: 588 1356 398 274 147 411 3214 Fragments arranged by size: 3214 1356 588 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2255 2285 2859 2889 1178 Size: 30 165 30 852 30 574 30 4677 Fragments arranged by size: 4677 852 574 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2276 2332 2363 2366 3463 1186 Size: 1090 56 31 3 1097 4111 Fragments arranged by size: 4111 1097 1090 56 31 3 BseYI C'CCAG_C Cuts at: 2121 2912 3635 5591 6087 2121 Size: 791 723 1956 496 2422 Fragments arranged by size: 2422 1956 791 723 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2087 1163 Size: 573 351 5464 Fragments arranged by size: 5464 573 351 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3838 4599 5627 1540 Size: 275 186 1837 761 1028 2301 Fragments arranged by size: 2301 1837 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2532 2855 3615 4449 4534 5605 2532 Size: 323 760 834 85 1071 3315 Fragments arranged by size: 3315 1071 834 760 323 85 BsmI GAATG_Cn' Cuts at: 3899 3992 3899 Size: 93 6295 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2056 2532 2577 2634 2674 2696 2786 Size: 399 266 476 45 57 40 22 90 Cuts at: 2786 2855 2925 3051 3141 3444 3615 3707 4449 Size: 69 70 126 90 303 171 92 742 Cuts at: 4449 4534 5605 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 476 399 303 266 171 126 92 90 90 85 70 69 57 45 40 22 BspEI T'CCGG_A Cuts at: 2877 3465 6051 2877 Size: 588 2586 3214 Fragments arranged by size: 3214 2586 588 BspHI T'CATG_A Cuts at: 4277 6007 4277 Size: 1730 4658 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2070 2109 2424 2824 3743 844 Size: 1226 39 315 400 919 3489 Fragments arranged by size: 3489 1226 919 400 315 39 BsrBI CCG'CTC Cuts at: 1394 1823 2476 4275 1394 Size: 429 653 1799 3507 Fragments arranged by size: 3507 1799 653 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4883 5057 65 Size: 1183 3635 174 1396 Fragments arranged by size: 3635 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2608 5032 5219 1700 Size: 110 798 2424 187 2869 Fragments arranged by size: 2869 2424 798 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6388 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6388 BssSI C'ACGA_G Cuts at: 4448 5460 4448 Size: 1012 5376 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6388 BstBI TT'CG_AA Cuts at: 5192 5192 Size: 6388 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6388 BstYI r'GATC_y Cuts at: 1972 3718 4085 4470 4487 5939 6025 6037 6383 Size: 1746 367 385 17 1452 86 12 346 Cuts at: 6383 1972 Size: 1977 Fragments arranged by size: 1977 1746 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3183 3183 Size: 6388 BtgI C'CryG_G Cuts at: 513 1065 2004 2508 2938 3144 3189 3387 513 Size: 552 939 504 430 206 45 198 3514 Fragments arranged by size: 3514 939 552 504 430 206 198 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5649 Fragments arranged by size: 5649 683 56 BtsI GCAGTG_nn' Cuts at: 2976 3359 3904 4698 4718 2976 Size: 383 545 794 20 4646 Fragments arranged by size: 4646 794 545 383 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6388 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6353 DraI TTT'AAA Cuts at: 3692 3692 Size: 6388 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5395 809 Size: 507 4079 1802 Fragments arranged by size: 4079 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 2392 2935 Size: 54 1425 50 275 18 255 307 543 Cuts at: 2935 2973 3835 4724 6353 8 Size: 38 862 889 1629 43 Fragments arranged by size: 1629 1425 889 862 543 307 275 255 54 50 43 38 18 EagI C'GGCC_G Cuts at: 1537 1812 3835 1537 Size: 275 2023 4090 Fragments arranged by size: 4090 2023 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2010 3090 4318 1353 Size: 657 1080 1228 3423 Fragments arranged by size: 3423 1228 1080 657 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4975 5349 5495 387 Size: 1064 138 3386 374 146 1280 Fragments arranged by size: 3386 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 2422 2576 2971 3104 3114 3188 Size: 393 402 388 154 395 133 10 74 Cuts at: 3188 3227 3590 4451 5048 5835 6103 1239 Size: 39 363 861 597 787 268 1524 Fragments arranged by size: 1524 861 787 597 402 395 393 388 363 268 154 133 74 39 10 EcoO109I rG'GnC_Cy Cuts at: 2692 2693 2773 2943 3100 3493 3580 2692 Size: 1 80 170 157 393 87 5500 Fragments arranged by size: 5500 393 170 157 87 80 1 EcoRI G'AATT_C Cuts at: 3697 4075 3697 Size: 378 6010 EcoRV GAT'ATC Cuts at: 2488 2488 Size: 6388 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2668 2668 Size: 6388 FspI TGC'GCA Cuts at: 2381 4894 2381 Size: 2513 3875 HaeII r_GCGC'y Cuts at: 1678 2220 5535 1678 Size: 542 3315 2531 Fragments arranged by size: 3315 2531 542 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2255 2287 2426 2458 2824 Size: 32 135 32 683 32 139 32 366 Cuts at: 2824 2856 3500 3520 3532 3552 5029 5061 5103 Size: 32 644 20 12 20 1477 32 42 Cuts at: 5103 5135 1373 Size: 32 2626 Fragments arranged by size: 2626 1477 683 644 366 139 135 42 32 32 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 3382 3732 3978 669 Size: 988 281 1444 350 246 3079 Fragments arranged by size: 3079 1444 988 350 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6388 HpaI GTT'AAC Cuts at: 3978 3978 Size: 6388 KasI G'GCGC_C Cuts at: 2216 2216 Size: 6388 KpnI G_GTAC'C Cuts at: 2121 2816 3713 2121 Size: 695 897 4796 Fragments arranged by size: 4796 897 695 MfeI C'AATT_G Cuts at: 3987 3987 Size: 6388 MluI A'CGCG_T Cuts at: 6264 6264 Size: 6388 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2298 5502 5686 1305 Size: 993 3204 184 2007 Fragments arranged by size: 3204 2007 993 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2087 2394 2937 2975 10 Size: 54 1425 343 255 307 543 38 3423 Fragments arranged by size: 3423 1425 543 343 307 255 54 38 MslI CAynn'nnrTG Cuts at: 518 1166 2535 3626 4346 4705 4864 518 Size: 648 1369 1091 720 359 159 2042 Fragments arranged by size: 2042 1369 1091 720 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2125 2131 3804 4481 5629 5874 1606 Size: 519 6 1673 677 1148 245 2120 Fragments arranged by size: 2120 1673 1148 677 519 245 6 NarI GG'CG_CC Cuts at: 2217 2217 Size: 6388 NcoI C'CATG_G Cuts at: 513 1065 2004 3189 513 Size: 552 939 1185 3712 Fragments arranged by size: 3712 1185 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6388 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6388 NotI GC'GGCC_GC Cuts at: 3835 3835 Size: 6388 NsiI A_TGCA'T Cuts at: 2025 2025 Size: 6388 NspI r_CATG'y Cuts at: 1132 1229 2665 3746 5291 1132 Size: 97 1436 1081 1545 2229 Fragments arranged by size: 2229 1545 1436 1081 97 PasI CC'CwG_GG Cuts at: 1348 2808 1348 Size: 1460 4928 PciI A'CATG_T Cuts at: 5287 5287 Size: 6388 PflMI CCAn_nnn'nTGG Cuts at: 1108 2744 1108 Size: 1636 4752 PfoI T'CCnGG_A Cuts at: 2895 2895 Size: 6388 PmeI GTTT'AAAC Cuts at: 3692 3692 Size: 6388 PmlI CAC'GTG Cuts at: 2325 3628 2325 Size: 1303 5085 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4457 4489 5970 6002 4457 Size: 32 1481 32 4843 Fragments arranged by size: 4843 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2773 3100 3493 3580 2773 Size: 327 393 87 5581 Fragments arranged by size: 5581 393 327 87 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6388 PsiI TTA'TAA Cuts at: 3958 3958 Size: 6388 PspOMI G'GGCC_C Cuts at: 2670 2692 2670 Size: 22 6366 PstI C_TGCA'G Cuts at: 830 2130 3740 830 Size: 1300 1610 3478 Fragments arranged by size: 3478 1610 1300 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6388 PvuII CAG'CTG Cuts at: 2125 2131 2125 Size: 6 6382 SalI G'TCGA_C Cuts at: 1936 3730 1936 Size: 1794 4594 SanDI GG'GwC_CC Cuts at: 2773 2773 Size: 6388 SbfI CC_TGCA'GG Cuts at: 3740 3740 Size: 6388 ScaI AGT'ACT Cuts at: 4636 6348 4636 Size: 1712 4676 SfcI C'TryA_G Cuts at: 826 1045 2126 3736 4871 5552 5743 826 Size: 219 1081 1610 1135 681 191 1471 Fragments arranged by size: 1610 1471 1135 1081 681 219 191 SfoI GGC'GCC Cuts at: 2218 2218 Size: 6388 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6388 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6388 SmaI CCC'GGG Cuts at: 1872 2669 3434 3715 1872 Size: 797 765 281 4545 Fragments arranged by size: 4545 797 765 281 SmlI C'TyrA_G Cuts at: 820 1017 1943 2585 3005 4492 5393 5655 820 Size: 197 926 642 420 1487 901 262 1553 Fragments arranged by size: 1553 1487 926 901 642 420 262 197 SnaBI TAC'GTA Cuts at: 493 4179 493 Size: 3686 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6388 SphI G_CATG'C Cuts at: 1132 3746 1132 Size: 2614 3774 SspI AAT'ATT Cuts at: 5 52 4312 5 Size: 47 4260 2081 Fragments arranged by size: 4260 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6388 StyI C'CwwG_G Cuts at: 513 1065 2004 2088 2421 2688 3107 3189 3683 Size: 552 939 84 333 267 419 82 494 Cuts at: 3683 3827 513 Size: 144 3074 Fragments arranged by size: 3074 939 552 494 419 333 267 144 84 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2619 3052 3361 3638 4415 4432 4585 Size: 1255 1039 433 309 277 777 17 153 Cuts at: 4585 4770 5215 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1039 777 445 433 309 277 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4634 6346 96 Size: 274 80 33 51 1443 2657 1712 138 Fragments arranged by size: 2657 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2765 2797 2859 2891 3120 3152 6348 6380 2765 Size: 32 62 32 229 32 3196 32 2773 Fragments arranged by size: 3196 2773 229 62 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2269 1316 Size: 953 5435 XbaI T'CTAG_A Cuts at: 3724 3724 Size: 6388 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6388 XmaI C'CCGG_G Cuts at: 1870 2667 3432 3713 1870 Size: 797 765 281 4545 Fragments arranged by size: 4545 797 765 281 XmnI GAAnn'nnTTC Cuts at: 4517 4517 Size: 6388 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4196 276 Size: 53 83 186 936 2662 2468 Fragments arranged by size: 2662 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FseI FspAI NaeI NgoMIV NruI PacI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI