Restriction map for
pFN21AB4221
(Linear) (Six-base) MAPSORT of: KIBB4221.seq Check: 862 from: 1 to: 1683
KIBB4221.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AccI GT'mk_AC
Cuts at: 0 173 1683
Size: 173 1510
Acc65I G'GTAC_C
Cuts at: 0 112 807 1683
Size: 112 695 876
Fragments arranged by size:
876 695 112
AclI AA'CG_TT
Cuts at: 0 275 1683
Size: 275 1408
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 1099 1109 1183 1683
Size: 29 1070 10 74 500
Fragments arranged by size:
1070 500 74 29 10
AleI CACnn'nnGTG
Cuts at: 0 1621 1683
Size: 1621 62
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1393 1425 1540 1572 1683
Size: 1393 32 115 32 111
Fragments arranged by size:
1393 115 111 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 57 129 989 1177 1644 1683
Size: 57 72 860 188 467 39
Fragments arranged by size:
860 467 188 72 57 39
ApaI G_GGCC'C
Cuts at: 0 669 691 1683
Size: 669 22 992
Fragments arranged by size:
992 669 22
AvaI C'yCGr_G
Cuts at: 0 662 780 1022 1427 1683
Size: 662 118 242 405 256
Fragments arranged by size:
662 405 256 242 118
AvrII C'CTAG_G
Cuts at: 0 1678 1683
Size: 1678 5
BanI G'GyrC_C
Cuts at: 0 46 112 211 807 1350 1655 1683
Size: 46 66 99 596 543 305 28
Fragments arranged by size:
596 543 305 99 66 46 28
BanII G_rGCy'C
Cuts at: 0 572 669 691 920 1046 1439 1683
Size: 572 97 22 229 126 393 244
Fragments arranged by size:
572 393 244 229 126 97 22
BbeI G_GCGC'C
Cuts at: 0 215 1683
Size: 215 1468
BbsI GAAGACnn'nnnn_
Cuts at: 0 521 1362 1683
Size: 521 841 321
Fragments arranged by size:
841 521 321
BbvCI CC'TCA_GC
Cuts at: 0 254 752 1683
Size: 254 498 931
Fragments arranged by size:
931 498 254
BfrBI ATG'CAT
Cuts at: 0 18 1683
Size: 18 1665
BglI GCCn_nnn'nGGC
Cuts at: 0 576 967 1683
Size: 576 391 716
Fragments arranged by size:
716 576 391
BlpI GC'TnA_GC
Cuts at: 0 791 1683
Size: 791 892
Bme1580I G_kGCm'C
Cuts at: 0 51 629 669 691 781 1136 1683
Size: 51 578 40 22 90 355 547
Fragments arranged by size:
578 547 355 90 51 40 22
BmgBI CAC'GTC
Cuts at: 0 266 1683
Size: 266 1417
BmrI ACTGGGnnnn_n'
Cuts at: 0 616 1682 1683
Size: 616 1066 1
Fragments arranged by size:
1066 616 1
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 558 576 1566 1683
Size: 558 18 990 117
Fragments arranged by size:
990 558 117 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 417 571 966 1222 1585 1683
Size: 417 154 395 256 363 98
Fragments arranged by size:
417 395 363 256 154 98
Bpu10I CC'TnA_GC
Cuts at: 0 254 719 752 1683
Size: 254 465 33 931
Fragments arranged by size:
931 465 254 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 985 1683
Size: 601 384 698
Fragments arranged by size:
698 601 384
BsaI GGTCTCn'nnnn_
Cuts at: 0 253 1683
Size: 253 1430
BsaAI yAC'GTr
Cuts at: 0 320 1623 1683
Size: 320 1303 60
Fragments arranged by size:
1303 320 60
BsaHI Gr'CG_yC
Cuts at: 0 212 1683
Size: 212 1471
BsaWI w'CCGG_w
Cuts at: 0 872 1460 1683
Size: 872 588 223
Fragments arranged by size:
872 588 223
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 250 280 854 884 1683
Size: 250 30 574 30 799
Fragments arranged by size:
799 574 250 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 327 358 361 1458 1683
Size: 271 56 31 3 1097 225
Fragments arranged by size:
1097 271 225 56 31 3
BseYI C'CCAG_C
Cuts at: 0 116 907 1630 1683
Size: 116 791 723 53
Fragments arranged by size:
791 723 116 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 1683
Size: 82 1601
BsiHKAI G_wGCw'C
Cuts at: 0 527 850 1610 1683
Size: 527 323 760 73
Fragments arranged by size:
760 527 323 73
Bsp1286I G_dGCh'C
Cuts at: 0 51 527 572 629 669 691 781 850
Size: 51 476 45 57 40 22 90 69
Cuts at: 850 920 1046 1136 1439 1610 1683
Size: 70 126 90 303 171 73
Fragments arranged by size:
476 303 171 126 90 90 73 70
69 57 51 45 40 22
BspEI T'CCGG_A
Cuts at: 0 872 1460 1683
Size: 872 588 223
Fragments arranged by size:
872 588 223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 65 104 419 819 1683
Size: 65 39 315 400 864
Fragments arranged by size:
864 400 315 65 39
BsrBI CCG'CTC
Cuts at: 0 471 1683
Size: 471 1212
BsrFI r'CCGG_y
Cuts at: 0 603 1683
Size: 603 1080
Bsu36I CC'TnA_GG
Cuts at: 0 1178 1683
Size: 1178 505
BtgI C'CryG_G
Cuts at: 0 503 933 1139 1184 1382 1683
Size: 503 430 206 45 198 301
Fragments arranged by size:
503 430 301 206 198 45
BtsI GCAGTG_nn'
Cuts at: 0 971 1354 1683
Size: 971 383 329
Fragments arranged by size:
971 383 329
EaeI y'GGCC_r
Cuts at: 0 80 387 930 968 1683
Size: 80 307 543 38 715
Fragments arranged by size:
715 543 307 80 38
EarI CTCTTCn'nnn_
Cuts at: 0 5 1085 1683
Size: 5 1080 598
Fragments arranged by size:
1080 598 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 417 571 966 1099 1109 1183 1222
Size: 29 388 154 395 133 10 74 39
Cuts at: 1222 1585 1683
Size: 363 98
Fragments arranged by size:
395 388 363 154 133 98 74 39
29 10
EcoO109I rG'GnC_Cy
Cuts at: 0 687 688 768 938 1095 1488 1575 1683
Size: 687 1 80 170 157 393 87 108
Fragments arranged by size:
687 393 170 157 108 87 80 1
EcoRV GAT'ATC
Cuts at: 0 483 1683
Size: 483 1200
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 663 1683
Size: 663 1020
FspI TGC'GCA
Cuts at: 0 376 1683
Size: 376 1307
HaeII r_GCGC'y
Cuts at: 0 215 1683
Size: 215 1468
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 250 282 421 453 819 851 1495 1515
Size: 250 32 139 32 366 32 644 20
Cuts at: 1515 1527 1547 1683
Size: 12 20 136
Fragments arranged by size:
644 366 250 139 136 32 32 32
20 20 12
HincII GTy'rAC
Cuts at: 0 1377 1683
Size: 1377 306
KasI G'GCGC_C
Cuts at: 0 211 1683
Size: 211 1472
KpnI G_GTAC'C
Cuts at: 0 116 811 1683
Size: 116 695 872
Fragments arranged by size:
872 695 116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 1683
Size: 293 1390
MscI TGG'CCA
Cuts at: 0 82 389 932 970 1683
Size: 82 307 543 38 713
Fragments arranged by size:
713 543 307 82 38
MslI CAynn'nnrTG
Cuts at: 0 530 1621 1683
Size: 530 1091 62
Fragments arranged by size:
1091 530 62
MspA1I CmG'CkG
Cuts at: 0 120 126 1683
Size: 120 6 1557
Fragments arranged by size:
1557 120 6
NarI GG'CG_CC
Cuts at: 0 212 1683
Size: 212 1471
NcoI C'CATG_G
Cuts at: 0 1184 1683
Size: 1184 499
NsiI A_TGCA'T
Cuts at: 0 20 1683
Size: 20 1663
NspI r_CATG'y
Cuts at: 0 660 1683
Size: 660 1023
PasI CC'CwG_GG
Cuts at: 0 803 1683
Size: 803 880
PflMI CCAn_nnn'nTGG
Cuts at: 0 739 1683
Size: 739 944
PfoI T'CCnGG_A
Cuts at: 0 890 1683
Size: 890 793
PmlI CAC'GTG
Cuts at: 0 320 1623 1683
Size: 320 1303 60
Fragments arranged by size:
1303 320 60
PpuMI rG'GwC_Cy
Cuts at: 0 768 1095 1488 1575 1683
Size: 768 327 393 87 108
Fragments arranged by size:
768 393 327 108 87
PspOMI G'GGCC_C
Cuts at: 0 665 687 1683
Size: 665 22 996
Fragments arranged by size:
996 665 22
PstI C_TGCA'G
Cuts at: 0 125 1683
Size: 125 1558
PvuII CAG'CTG
Cuts at: 0 120 126 1683
Size: 120 6 1557
Fragments arranged by size:
1557 120 6
SanDI GG'GwC_CC
Cuts at: 0 768 1683
Size: 768 915
SfcI C'TryA_G
Cuts at: 0 121 1683
Size: 121 1562
SfoI GGC'GCC
Cuts at: 0 213 1683
Size: 213 1470
SmaI CCC'GGG
Cuts at: 0 664 1429 1683
Size: 664 765 254
Fragments arranged by size:
765 664 254
SmlI C'TyrA_G
Cuts at: 0 580 1000 1683
Size: 580 420 683
Fragments arranged by size:
683 580 420
StyI C'CwwG_G
Cuts at: 0 83 416 683 1102 1184 1678 1683
Size: 83 333 267 419 82 494 5
Fragments arranged by size:
494 419 333 267 83 82 5
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 614 1047 1356 1633 1683
Size: 614 433 309 277 50
Fragments arranged by size:
614 433 309 277 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 760 792 854 886 1115 1147 1683
Size: 760 32 62 32 229 32 536
Fragments arranged by size:
760 536 229 62 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 264 1683
Size: 264 1419
XmaI C'CCGG_G
Cuts at: 0 662 1427 1683
Size: 662 765 256
Fragments arranged by size:
765 662 256
Enzymes that do cut:
AccI Acc65I AclI AcuI AleI AloI AlwNI ApaI
AvaI AvrII BanI BanII BbeI BbsI BbvCI BfrBI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI Bsu36I
BtgI BtsI EaeI EarI Eco57MI EcoO109I EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NarI NcoI NsiI NspI PasI PflMI
PfoI PmlI PpuMI PspOMI PstI PvuII SanDI SfcI
SfoI SmaI SmlI StyI TaqII TstI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AflIII AgeI AhdI AlfI
ApaLI ApoI AscI AseI AsiSI BaeI BamHI BcgI
BciVI BclI BglII BmtI BsaBI BsiEI BsiWI BsmI
BsmBI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI FseI
FspAI HindIII HpaI MfeI MluI NaeI NdeI NgoMIV
NheI NotI NruI PacI PciI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI XbaI
XcmI XhoI XmnI ZraI