Restriction map for
pFN21AB4188
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BclI BcnI BfrI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI HphI KasI KpnI
Ksp632I KspI MfeI MluI MroI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SspBI SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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ApaI 1041 GGGCCC GGGCC^C
Asp700I 574 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 569 CTCGAG C^[CT]CG[GA]G
AvaII 1003 GGTCC G^G[AT]CC
BbsI 1028 GAAGAC GAAGAC(2/6)
BbvCI 448 GCTGAGG CCTCAGC(-5/-2)
BciVI 802 GGATAC GTATCC(6/5)
BglI 577 GCCTTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 44 AGATCT A^GATCT
BpmI 266 CTCCAG CTGGAG(16/14)
BpuAI 1028 GAAGAC GAAGAC(2/6)
BsaBI 468 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 263 GGTCTC GGTCTC(1/5)
BsgI 622 GTGCAG GTGCAG(16/14)
BsiYI 177 CCTGTTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 177 CCTGTTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 3 GTCCC GGGAC(10/14)
BsmI 228 GAATGC GAATGC(1/-1)
BsoBI 569 CTCGAG C^[CT]CG[GA]G
BsrBI 823 GAGCGG CCGCTC(-3/-3)
BsrDI 255 CATTGC GCAATG(2/0)
BsrFI 13 ACCGGC [GA]^CCGG[CT]
BstBI 1025 TTCGAA TT^CGAA
BstYI 44 AGATCT [GA]^GATC[CT]
Cac8I 969 GCATGC GC[ACGT]^[ACGT]GC
Cfr10I 13 ACCGGC [GA]^CCGG[CT]
EcoNI 177 CCTGTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1022 GAATTC G^AATTC
EcoT14I 710 CCAAGG C^C[AT][AT]GG
HapII 14 CCGG C^CGG
HincII 927 GTTGAC GT[CT]^[GA]AC
HindII 927 GTTGAC GT[CT]^[GA]AC
HpaII 14 CCGG C^CGG
MaeII 515 ACGT A^CGT
MamI 468 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 44 AGATCT [GA]^GATC[CT]
MspI 14 CCGG C^CGG
NspV 1025 TTCGAA TT^CGAA
PaeR7I 569 CTCGAG C^TCGAG
PleI 772 GAGTC GAGTC(4/5)
PpuMI 1002 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 446 CAGCTG CAG^CTG
SbfI 1006 CCTGCAGG CCTGCA^GG
SfuI 1025 TTCGAA TT^CGAA
SmlI 569 CTCGAG C^T[CT][GA]AG
SphI 969 GCATGC GCATG^C
Sse8387I 1006 CCTGCAGG CCTGCA^GG
StyI 710 CCAAGG C^C[AT][AT]GG
Tsp45I 417 GTCAC ^GT[GC]AC
TspRI 437 CACTG CA[GC]TG[ACGT][ACGT]^
XhoI 569 CTCGAG C^TCGAG
XhoII 44 AGATCT [GA]^GATC[CT]
XmnI 574 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 825 GCGG CCGC(-3/-1)
1048 GCGG
AcsI 331 GAATTT [GA]^AATT[CT]
1022 GAATTC
AlwI 118 GGATC GGATC(4/5)
492 GGATC
ApoI 331 GAATTT [GA]^AATT[CT]
1022 GAATTC
BalI 342 TGGCCA TGG^CCA
662 TGGCCA
BanII 483 GAGCCC G[GA]GC[CT]^C
1041 GGGCCC
BfaI 839 CTAG C^TAG
859 CTAG
BmyI 483 GAGCCC G[AGT]GC[ACT]^C
1041 GGGCCC
BsmAI 761 GAGAC GTCTC(1/5)
264 GTCTC
Bsp1286I 483 GAGCCC G[AGT]GC[ACT]^C
1041 GGGCCC
DraII 1002 AGGTCCT [GA]G^G[ACGT]CC[CT]
1040 GGGGCCC
EaeI 342 TGGCCA [CT]^GGCC[GA]
662 TGGCCA
EcoO109I 1002 AGGTCCT [GA]G^G[ACGT]CC[CT]
1040 GGGGCCC
MaeI 839 CTAG C^TAG
859 CTAG
MaeIII 417 GTCAC ^GT[ACGT]AC
725 GTTAC
MluNI 342 TGGCCA TGG^CCA
662 TGGCCA
MscI 342 TGGCCA TGG^CCA
662 TGGCCA
MslI 274 CATTCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
748 CATTCCCATG
MspA1I 446 CAGCTG C[AC]G^C[GT]G
1046 CAGCGG
NlaIV 1040 GGGGCC GG[ACGT]^[ACGT]CC
1041 GGGCCC
NspI 808 ACATGC [GA]CATG^[CT]
969 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AfaI AluI BbvI BsaJI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp509I TthHB8I