(Circular) (Six-base) MAPSORT of: pFN21AB4165.seq Check: 6987 from: 1 to: 7183 pFN21AB4165 7183 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2848 2856 2866 3157 3453 2848 Size: 8 10 291 296 6578 Fragments arranged by size: 6578 296 291 10 8 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4125 4993 278 Size: 53 83 186 936 2589 868 2468 Fragments arranged by size: 2589 2468 936 868 186 83 53 AccI GT'mk_AC Cuts at: 1937 4526 1937 Size: 2589 4594 Acc65I G'GTAC_C Cuts at: 4504 4504 Size: 7183 AclI AA'CG_TT Cuts at: 1563 5310 5683 1563 Size: 3747 373 3063 Fragments arranged by size: 3747 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2351 2903 5246 6630 6898 1632 Size: 719 552 2343 1384 268 1917 Fragments arranged by size: 2343 1917 1384 719 552 268 AfeI AGC'GCT Cuts at: 3724 3724 Size: 7183 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6986 AflIII A'CryG_T Cuts at: 1203 3516 6082 7059 1203 Size: 2313 2566 977 1327 Fragments arranged by size: 2566 2313 1327 977 AgeI A'CCGG_T Cuts at: 6014 6014 Size: 7183 AhdI GACnn_n'nnGTC Cuts at: 2554 5912 2554 Size: 3358 3825 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7183 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2521 2609 4091 1184 Size: 1337 88 1482 4276 Fragments arranged by size: 4276 1482 1337 88 AlwNI CAG_nnn'CTG Cuts at: 2848 2881 2902 2989 3007 3265 3538 4926 6498 Size: 33 21 87 18 258 273 1388 1572 Cuts at: 6498 2848 Size: 3533 Fragments arranged by size: 3533 1572 1388 273 258 87 33 21 18 ApaI G_GGCC'C Cuts at: 2080 2752 2977 3658 4414 2080 Size: 672 225 681 756 4849 Fragments arranged by size: 4849 756 681 672 225 ApaLI G'TGCA_C Cuts at: 3074 5240 6396 3074 Size: 2166 1156 3861 Fragments arranged by size: 3861 2166 1156 ApoI r'AATT_y Cuts at: 1493 4492 4718 4870 6979 1493 Size: 2999 226 152 2109 1697 Fragments arranged by size: 2999 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5737 160 Size: 872 4705 1606 Fragments arranged by size: 4705 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7183 AvaI C'yCGr_G Cuts at: 1870 1943 2087 2158 4508 1870 Size: 73 144 71 2350 4545 Fragments arranged by size: 4545 2350 144 73 71 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2181 2214 3625 3658 4223 4256 2181 Size: 33 1411 33 565 33 5108 Fragments arranged by size: 5108 1411 565 33 33 33 BamHI G'GATC_C Cuts at: 4451 4513 4880 4451 Size: 62 367 6754 Fragments arranged by size: 6754 367 62 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2390 2709 3596 4504 5959 Size: 325 217 627 603 319 887 908 1455 Cuts at: 5959 618 Size: 1842 Fragments arranged by size: 1842 1455 908 887 627 603 325 319 217 BanII G_rGCy'C Cuts at: 1391 2080 2088 2263 2304 2752 2912 2977 3055 Size: 689 8 175 41 448 160 65 78 Cuts at: 3055 3159 3321 3658 4414 4502 1391 Size: 104 162 337 756 88 4072 Fragments arranged by size: 4072 756 689 448 337 175 162 160 104 88 78 65 41 8 BbeI G_GCGC'C Cuts at: 2713 3600 2713 Size: 887 6296 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2633 3481 928 Size: 971 734 848 4630 Fragments arranged by size: 4630 971 848 734 BbvCI CC'TCA_GC Cuts at: 2643 2819 2643 Size: 176 7007 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2717 2751 5374 5408 1903 Size: 34 780 34 2623 34 3678 Fragments arranged by size: 3678 2623 780 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5075 6285 5075 Size: 1210 5973 BclI T'GATC_A Cuts at: 1548 1548 Size: 7183 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2129 3594 3807 Size: 107 122 71 631 605 457 1465 213 Cuts at: 3807 5794 136 Size: 1987 1525 Fragments arranged by size: 1987 1525 1465 631 605 457 213 122 107 71 BglII A'GATC_T Cuts at: 3732 7178 3732 Size: 3446 3737 BlpI GC'TnA_GC Cuts at: 4600 4600 Size: 7183 Bme1580I G_kGCm'C Cuts at: 1790 2080 2224 2393 2752 2977 3078 3658 3803 Size: 290 144 169 359 225 101 580 145 Cuts at: 3803 4161 4414 5244 6400 1790 Size: 358 253 830 1156 2573 Fragments arranged by size: 2573 1156 830 580 359 358 290 253 225 169 145 144 101 BmgBI CAC'GTC Cuts at: 1318 2025 3517 1318 Size: 707 1492 4984 Fragments arranged by size: 4984 1492 707 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2184 2189 2284 Size: 416 53 83 399 24 767 5 95 Cuts at: 2284 2746 3709 4914 5872 442 Size: 462 963 1205 958 1753 Fragments arranged by size: 1753 1205 963 958 767 462 416 399 95 83 53 24 5 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7183 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3952 3952 Size: 7183 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2200 2591 2987 3011 3806 5843 1239 Size: 961 391 396 24 795 2037 2579 Fragments arranged by size: 2579 2037 961 795 396 391 24 Bpu10I CC'TnA_GC Cuts at: 2264 2643 2819 2264 Size: 379 176 6628 Fragments arranged by size: 6628 379 176 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2645 3143 4265 5308 6173 6471 2645 Size: 498 1122 1043 865 298 3357 Fragments arranged by size: 3357 1122 1043 865 498 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2723 5846 882 Size: 617 1224 3123 2219 Fragments arranged by size: 3123 2219 1224 617 BsaAI yAC'GTr Cuts at: 493 1204 4156 4974 493 Size: 711 2952 818 2702 Fragments arranged by size: 2952 2702 818 711 BsaBI GATnn'nnATC Cuts at: 7177 7177 Size: 7183 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2710 3093 3597 3692 Size: 53 83 186 936 1177 383 504 95 Cuts at: 3692 4122 4391 4990 5372 6007 275 Size: 430 269 599 382 635 1451 Fragments arranged by size: 1451 1177 936 635 599 504 430 383 382 269 186 95 83 53 BsaWI w'CCGG_w Cuts at: 3096 4458 5616 6014 6288 6435 6846 3096 Size: 1362 1158 398 274 147 411 3433 Fragments arranged by size: 3433 1362 1158 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2198 2228 2780 2810 2987 Size: 30 165 30 795 30 552 30 177 Cuts at: 2987 3017 3416 3446 3677 3707 1178 Size: 30 399 30 231 30 4654 Fragments arranged by size: 4654 795 552 399 231 177 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2705 2708 3008 3050 3254 1186 Size: 1519 3 300 42 204 5115 Fragments arranged by size: 5115 1519 300 204 42 3 BseYI C'CCAG_C Cuts at: 2113 2119 2239 2383 2538 2559 4312 4424 6386 Size: 6 120 144 155 21 1753 112 1962 Cuts at: 6386 6882 2113 Size: 496 2414 Fragments arranged by size: 2414 1962 1753 496 155 144 120 112 21 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2250 2639 3434 3551 3701 3872 1163 Size: 573 514 389 795 117 150 171 4474 Fragments arranged by size: 4474 795 573 514 389 171 150 117 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2133 2635 3909 4482 4633 5394 Size: 275 186 132 502 1274 573 151 761 Cuts at: 5394 6422 1540 Size: 1028 2301 Fragments arranged by size: 2301 1274 1028 761 573 502 275 186 151 132 BsiHKAI G_wGCw'C Cuts at: 2523 2912 3078 3321 3933 5244 5329 6400 2523 Size: 389 166 243 612 1311 85 1071 3306 Fragments arranged by size: 3306 1311 1071 612 389 243 166 85 BsiWI C'GTAC_G Cuts at: 3582 3582 Size: 7183 BsmI GAATG_Cn' Cuts at: 4694 4787 4694 Size: 93 7090 BsmBI CGTCTCn'nnnn_ Cuts at: 4259 4259 Size: 7183 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2080 2088 2224 2263 2304 2393 2523 Size: 399 290 8 136 39 41 89 130 Cuts at: 2523 2752 2912 2977 3055 3078 3159 3321 3658 Size: 229 160 65 78 23 81 162 337 Cuts at: 3658 3803 3933 4161 4414 4502 5244 5329 6400 Size: 145 130 228 253 88 742 85 1071 Cuts at: 6400 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 337 290 253 229 228 162 160 145 136 130 130 89 88 85 81 78 65 41 39 23 8 BspEI T'CCGG_A Cuts at: 3096 4458 6846 3096 Size: 1362 2388 3433 Fragments arranged by size: 3433 2388 1362 BspHI T'CATG_A Cuts at: 5072 6802 5072 Size: 1730 5453 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2308 2424 2489 2848 2856 2866 3157 3453 Size: 1464 116 65 359 8 10 291 296 Cuts at: 3453 3765 4194 4538 844 Size: 312 429 344 3489 Fragments arranged by size: 3489 1464 429 359 344 312 296 291 116 65 10 8 BsrBI CCG'CTC Cuts at: 1394 1823 2698 2971 3942 5070 1394 Size: 429 875 273 971 1128 3507 Fragments arranged by size: 3507 1128 971 875 429 273 BsrDI GCAATG_nn' Cuts at: 65 1248 2733 4125 5678 5852 65 Size: 1183 1485 1392 1553 174 1396 Fragments arranged by size: 1553 1485 1396 1392 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2153 2634 3121 3599 4378 5827 6014 Size: 110 343 481 487 478 779 1449 187 Cuts at: 6014 1700 Size: 2869 Fragments arranged by size: 2869 1449 779 487 481 478 343 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7183 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7183 BssSI C'ACGA_G Cuts at: 2726 3077 3320 3354 5243 6255 2726 Size: 351 243 34 1889 1012 3654 Fragments arranged by size: 3654 1889 1012 351 243 34 BstAPI GCAn_nnn'nTGC Cuts at: 794 2452 2482 3952 794 Size: 1658 30 1470 4025 Fragments arranged by size: 4025 1658 1470 30 BstBI TT'CG_AA Cuts at: 5987 5987 Size: 7183 BstEII G'GTnAC_C Cuts at: 1184 3541 1184 Size: 2357 4826 BstYI r'GATC_y Cuts at: 1972 3732 3789 4451 4513 4880 5265 5282 6734 Size: 1760 57 662 62 367 385 17 1452 Cuts at: 6734 6820 6832 7178 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1760 1452 662 385 367 346 86 62 57 17 12 Bsu36I CC'TnA_GG Cuts at: 3203 3393 3749 3774 3203 Size: 190 356 25 6612 Fragments arranged by size: 6612 356 190 25 BtgI C'CryG_G Cuts at: 513 1065 2004 2296 2977 4002 4245 513 Size: 552 939 292 681 1025 243 3451 Fragments arranged by size: 3451 1025 939 681 552 292 243 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6444 Fragments arranged by size: 6444 683 56 BtsI GCAGTG_nn' Cuts at: 3125 3182 3817 4699 5493 5513 3125 Size: 57 635 882 794 20 4795 Fragments arranged by size: 4795 882 794 635 57 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7183 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7148 DraI TTT'AAA Cuts at: 4487 4487 Size: 7183 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6190 809 Size: 507 4874 1802 Fragments arranged by size: 4874 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2130 3362 3586 Size: 54 1425 50 275 18 300 1232 224 Cuts at: 3586 3889 4479 4630 5519 7148 8 Size: 303 590 151 889 1629 43 Fragments arranged by size: 1629 1425 1232 889 590 303 300 275 224 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2130 4479 4630 1537 Size: 275 318 2349 151 4090 Fragments arranged by size: 4090 2349 318 275 151 EarI CTCTTCn'nnn_ Cuts at: 1353 3006 3674 5113 1353 Size: 1653 668 1439 3423 Fragments arranged by size: 3423 1653 1439 668 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2594 5770 6144 6290 387 Size: 1064 138 1005 3176 374 146 1280 Fragments arranged by size: 3176 1280 1064 1005 374 146 138 EcoICRI GAG'CTC Cuts at: 2910 3319 2910 Size: 409 6774 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2200 2351 2591 2903 2987 3011 3806 Size: 393 568 151 240 312 84 24 795 Cuts at: 3806 5246 5843 6630 6898 1239 Size: 1440 597 787 268 1524 Fragments arranged by size: 1524 1440 795 787 597 568 393 312 268 240 151 84 24 EcoNI CCTnn'n_nnAGG Cuts at: 2017 3011 4079 4190 2017 Size: 994 1068 111 5010 Fragments arranged by size: 5010 1068 994 111 EcoO109I rG'GnC_Cy Cuts at: 2056 2077 2413 2501 2815 3199 3389 3535 3655 Size: 21 336 88 314 384 190 146 120 Cuts at: 3655 3745 3754 4316 4344 2056 Size: 90 9 562 28 4895 Fragments arranged by size: 4895 562 384 336 314 190 146 120 90 88 28 21 9 EcoRI G'AATT_C Cuts at: 4492 4870 4492 Size: 378 6805 FspI TGC'GCA Cuts at: 2486 3272 5689 2486 Size: 786 2417 3980 Fragments arranged by size: 3980 2417 786 HaeII r_GCGC'y Cuts at: 1678 2461 2538 2713 3600 3726 6330 1678 Size: 783 77 175 887 126 2604 2531 Fragments arranged by size: 2604 2531 887 783 175 126 77 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2198 2230 2540 2572 2780 Size: 32 135 32 626 32 310 32 208 Cuts at: 2780 2812 2987 3019 3416 3448 3677 3709 4328 Size: 32 175 32 397 32 229 32 619 Cuts at: 4328 4360 5824 5856 5898 5930 1373 Size: 32 1464 32 42 32 2626 Fragments arranged by size: 2626 1464 626 619 397 310 229 208 175 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3247 4527 4773 669 Size: 988 281 1309 1280 246 3079 Fragments arranged by size: 3079 1309 1280 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7183 HpaI GTT'AAC Cuts at: 4773 4773 Size: 7183 KasI G'GCGC_C Cuts at: 2709 3596 2709 Size: 887 6296 KpnI G_GTAC'C Cuts at: 4508 4508 Size: 7183 MfeI C'AATT_G Cuts at: 4782 4782 Size: 7183 MluI A'CGCG_T Cuts at: 7059 7059 Size: 7183 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6297 6481 1305 Size: 4992 184 2007 Fragments arranged by size: 4992 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3891 10 Size: 54 1425 343 2059 3302 Fragments arranged by size: 3302 2059 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3344 5141 5500 5659 518 Size: 648 2178 1797 359 159 2042 Fragments arranged by size: 2178 2042 1797 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2563 2569 2902 2920 3295 3629 3652 3664 Size: 957 6 333 18 375 334 23 12 Cuts at: 3664 3820 4599 5276 6424 6669 1606 Size: 156 779 677 1148 245 2120 Fragments arranged by size: 2120 1148 957 779 677 375 334 333 245 156 23 18 12 6 NaeI GCC'GGC Cuts at: 3123 3601 4380 3123 Size: 478 779 5926 Fragments arranged by size: 5926 779 478 NarI GG'CG_CC Cuts at: 2710 3597 2710 Size: 887 6296 NcoI C'CATG_G Cuts at: 513 1065 2004 2977 513 Size: 552 939 973 4719 Fragments arranged by size: 4719 973 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7183 NgoMIV G'CCGG_C Cuts at: 3121 3599 4378 3121 Size: 478 779 5926 Fragments arranged by size: 5926 779 478 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7183 NotI GC'GGCC_GC Cuts at: 4630 4630 Size: 7183 NspI r_CATG'y Cuts at: 1132 1229 2596 4541 6086 1132 Size: 97 1367 1945 1545 2229 Fragments arranged by size: 2229 1945 1545 1367 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7183 PciI A'CATG_T Cuts at: 6082 6082 Size: 7183 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7183 PfoI T'CCnGG_A Cuts at: 3522 3522 Size: 7183 PmeI GTTT'AAAC Cuts at: 4487 4487 Size: 7183 PmlI CAC'GTG Cuts at: 4156 4156 Size: 7183 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5252 5284 6765 6797 5252 Size: 32 1481 32 5638 Fragments arranged by size: 5638 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2413 2501 3389 3745 3754 4344 2413 Size: 88 888 356 9 590 5252 Fragments arranged by size: 5252 888 590 356 88 9 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7183 PsiI TTA'TAA Cuts at: 4753 4753 Size: 7183 PspOMI G'GGCC_C Cuts at: 2076 2748 2973 3654 4410 2076 Size: 672 225 681 756 4849 Fragments arranged by size: 4849 756 681 672 225 PstI C_TGCA'G Cuts at: 830 2319 2568 2853 2907 3195 3294 3663 3986 Size: 1489 249 285 54 288 99 369 323 Cuts at: 3986 4194 4473 4535 830 Size: 208 279 62 3478 Fragments arranged by size: 3478 1489 369 323 288 285 279 249 208 99 62 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7183 PvuII CAG'CTG Cuts at: 2563 2569 2902 2920 3295 3664 3820 2563 Size: 6 333 18 375 369 156 5926 Fragments arranged by size: 5926 375 369 333 156 18 6 SacI G_AGCT'C Cuts at: 2912 3321 2912 Size: 409 6774 SalI G'TCGA_C Cuts at: 1936 4525 1936 Size: 2589 4594 SapI GCTCTTCn'nnn_ Cuts at: 3674 3674 Size: 7183 SbfI CC_TGCA'GG Cuts at: 2319 2853 4535 2319 Size: 534 1682 4967 Fragments arranged by size: 4967 1682 534 ScaI AGT'ACT Cuts at: 5431 7143 5431 Size: 1712 5471 SexAI A'CCwGG_T Cuts at: 4017 4017 Size: 7183 SfcI C'TryA_G Cuts at: 826 1045 2315 2564 2849 2903 3191 3290 3659 Size: 219 1270 249 285 54 288 99 369 Cuts at: 3659 3982 4190 4469 4531 5666 6347 6538 826 Size: 323 208 279 62 1135 681 191 1471 Fragments arranged by size: 1471 1270 1135 681 369 323 288 285 279 249 219 208 191 99 62 54 SfoI GGC'GCC Cuts at: 2711 3598 2711 Size: 887 6296 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7183 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7183 SmaI CCC'GGG Cuts at: 1872 2160 4510 1872 Size: 288 2350 4545 Fragments arranged by size: 4545 2350 288 SmlI C'TyrA_G Cuts at: 820 1017 1943 2660 3158 4280 5287 6188 6450 Size: 197 926 717 498 1122 1007 901 262 Cuts at: 6450 820 Size: 1553 Fragments arranged by size: 1553 1122 1007 926 901 717 498 262 197 SnaBI TAC'GTA Cuts at: 493 4974 493 Size: 4481 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7183 SphI G_CATG'C Cuts at: 1132 2596 4541 1132 Size: 1464 1945 3774 Fragments arranged by size: 3774 1945 1464 SspI AAT'ATT Cuts at: 5 52 5107 5 Size: 47 5055 2081 Fragments arranged by size: 5055 2081 47 StuI AGG'CCT Cuts at: 1517 3041 3503 3993 1517 Size: 1524 462 490 4707 Fragments arranged by size: 4707 1524 490 462 StyI C'CwwG_G Cuts at: 513 1065 2004 2977 4622 513 Size: 552 939 973 1645 3074 Fragments arranged by size: 3074 1645 973 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2645 3923 5210 5227 5380 5565 6010 Size: 1255 1065 1278 1287 17 153 185 445 Cuts at: 6010 325 Size: 1498 Fragments arranged by size: 1498 1287 1278 1255 1065 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3705 5429 7141 Size: 274 80 33 51 1443 1728 1724 1712 Cuts at: 7141 96 Size: 138 Fragments arranged by size: 1728 1724 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7143 7175 7143 Size: 32 7151 Tth111I GACn'n_nGTC Cuts at: 1316 3485 1316 Size: 2169 5014 XbaI T'CTAG_A Cuts at: 4366 4519 4366 Size: 153 7030 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3055 3055 Size: 7183 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7183 XmaI C'CCGG_G Cuts at: 1870 2158 4508 1870 Size: 288 2350 4545 Fragments arranged by size: 4545 2350 288 XmnI GAAnn'nnTTC Cuts at: 5312 5312 Size: 7183 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4123 4991 276 Size: 53 83 186 936 2589 868 2468 Fragments arranged by size: 2589 2468 936 868 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BfrBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI