(Circular) (Six-base) MAPSORT of: pFN21AB4139.seq Check: 8535 from: 1 to: 6013 pFN21AB4139 6013 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2673 2673 Size: 6013 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3823 278 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3356 1937 Size: 1419 4594 Acc65I G'GTAC_C Cuts at: 3334 3334 Size: 6013 AclI AA'CG_TT Cuts at: 1563 4140 4513 1563 Size: 2577 373 3063 Fragments arranged by size: 3063 2577 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2209 3124 4076 5460 5728 1632 Size: 577 915 952 1384 268 1917 Fragments arranged by size: 1917 1384 952 915 577 268 AfeI AGC'GCT Cuts at: 2050 2050 Size: 6013 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5816 AflIII A'CryG_T Cuts at: 1203 4912 5889 1203 Size: 3709 977 1327 Fragments arranged by size: 3709 1327 977 AgeI A'CCGG_T Cuts at: 4844 4844 Size: 6013 AhdI GACnn_n'nnGTC Cuts at: 4742 4742 Size: 6013 AleI CACnn'nnGTG Cuts at: 1166 2511 3015 1166 Size: 1345 504 4164 Fragments arranged by size: 4164 1345 504 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6013 AlwNI CAG_nnn'CTG Cuts at: 3756 5328 3756 Size: 1572 4441 ApaLI G'TGCA_C Cuts at: 4070 5226 4070 Size: 1156 4857 ApoI r'AATT_y Cuts at: 1493 2012 2174 2418 2502 2754 2838 3006 3090 Size: 519 162 244 84 252 84 168 84 Cuts at: 3090 3174 3322 3548 3700 5809 1493 Size: 84 148 226 152 2109 1697 Fragments arranged by size: 2109 1697 519 252 244 226 168 162 152 148 84 84 84 84 AseI AT'TA_AT Cuts at: 160 1032 4567 160 Size: 872 3535 1606 Fragments arranged by size: 3535 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 AvaI C'yCGr_G Cuts at: 1870 1943 3338 1870 Size: 73 1395 4545 Fragments arranged by size: 4545 1395 73 BamHI G'GATC_C Cuts at: 3343 3710 3343 Size: 367 5646 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3334 4789 618 Size: 325 217 627 1547 1455 1842 Fragments arranged by size: 1842 1547 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3332 1391 Size: 1941 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2972 928 Size: 971 1073 3969 Fragments arranged by size: 3969 1073 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4204 4238 1903 Size: 34 2267 34 3678 Fragments arranged by size: 3678 2267 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3905 5115 3905 Size: 1210 4803 BclI T'GATC_A Cuts at: 1548 1548 Size: 6013 BfrBI ATG'CAT Cuts at: 3036 3036 Size: 6013 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4624 136 Size: 107 122 71 631 605 2952 1525 Fragments arranged by size: 2952 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6008 6008 Size: 6013 BlpI GC'TnA_GC Cuts at: 3430 3430 Size: 6013 Bme1580I G_kGCm'C Cuts at: 1790 4074 5230 1790 Size: 2284 1156 2573 Fragments arranged by size: 2573 2284 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6013 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3744 4702 442 Size: 416 53 83 399 24 2327 958 1753 Fragments arranged by size: 2327 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6013 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2212 2212 Size: 6013 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2700 4673 1239 Size: 1461 1973 2579 Fragments arranged by size: 2579 1973 1461 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2154 2800 4138 5003 5301 2154 Size: 646 1338 865 298 2866 Fragments arranged by size: 2866 1338 865 646 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4676 882 Size: 617 3177 2219 Fragments arranged by size: 3177 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3804 493 Size: 711 2600 2702 Fragments arranged by size: 2702 2600 711 BsaBI GATnn'nnATC Cuts at: 6007 6007 Size: 6013 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3820 4202 4837 275 Size: 53 83 186 936 2287 382 635 1451 Fragments arranged by size: 2287 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4446 4844 5118 5265 5676 4446 Size: 398 274 147 411 4783 Fragments arranged by size: 4783 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5788 Fragments arranged by size: 5788 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2111 3275 1186 Size: 925 1164 3924 Fragments arranged by size: 3924 1164 925 BseYI C'CCAG_C Cuts at: 5216 5712 5216 Size: 496 5517 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5440 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3463 4224 5252 1540 Size: 275 186 1462 761 1028 2301 Fragments arranged by size: 2301 1462 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4074 4159 5230 4074 Size: 85 1071 4857 Fragments arranged by size: 4857 1071 85 BsmI GAATG_Cn' Cuts at: 2297 3038 3524 3617 2297 Size: 741 486 93 4693 Fragments arranged by size: 4693 741 486 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3332 4074 4159 5230 1391 Size: 399 1542 742 85 1071 2174 Fragments arranged by size: 2174 1542 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5676 5676 Size: 6013 BspHI T'CATG_A Cuts at: 2869 3028 3902 5632 2869 Size: 159 874 1730 3250 Fragments arranged by size: 3250 1730 874 159 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2673 3368 844 Size: 1829 695 3489 Fragments arranged by size: 3489 1829 695 BsrBI CCG'CTC Cuts at: 1394 1823 3900 1394 Size: 429 2077 3507 Fragments arranged by size: 3507 2077 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2365 2392 2627 4508 4682 65 Size: 1183 1117 27 235 1881 174 1396 Fragments arranged by size: 1881 1396 1183 1117 235 174 27 BsrFI r'CCGG_y Cuts at: 1700 1810 4657 4844 1700 Size: 110 2847 187 2869 Fragments arranged by size: 2869 2847 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6013 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6013 BssSI C'ACGA_G Cuts at: 4073 5085 4073 Size: 1012 5001 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6013 BstBI TT'CG_AA Cuts at: 4817 4817 Size: 6013 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6013 BstYI r'GATC_y Cuts at: 1972 3343 3710 4095 4112 5564 5650 5662 6008 Size: 1371 367 385 17 1452 86 12 346 Cuts at: 6008 1972 Size: 1977 Fragments arranged by size: 1977 1452 1371 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4522 Fragments arranged by size: 4522 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2711 487 Size: 683 56 1485 3789 Fragments arranged by size: 3789 1485 683 56 BtsI GCAGTG_nn' Cuts at: 3529 4323 4343 3529 Size: 794 20 5199 Fragments arranged by size: 5199 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6013 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5978 DraI TTT'AAA Cuts at: 2017 2326 3317 2017 Size: 309 991 4713 Fragments arranged by size: 4713 991 309 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5020 809 Size: 507 3704 1802 Fragments arranged by size: 3704 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3460 4349 5978 Size: 54 1425 50 275 18 1630 889 1629 Cuts at: 5978 8 Size: 43 Fragments arranged by size: 1630 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3460 1537 Size: 275 1648 4090 Fragments arranged by size: 4090 1648 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3255 3943 1353 Size: 1902 688 3423 Fragments arranged by size: 3423 1902 688 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4600 4974 5120 387 Size: 1064 138 3011 374 146 1280 Fragments arranged by size: 3011 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2209 2700 3124 4076 4673 5460 5728 Size: 393 577 491 424 952 597 787 268 Cuts at: 5728 1239 Size: 1524 Fragments arranged by size: 1524 952 787 597 577 491 424 393 268 EcoRI G'AATT_C Cuts at: 2418 2502 2754 3006 3090 3174 3322 3700 2418 Size: 84 252 252 84 84 148 378 4731 Fragments arranged by size: 4731 378 252 252 148 84 84 84 FspI TGC'GCA Cuts at: 4519 4519 Size: 6013 HaeII r_GCGC'y Cuts at: 1678 2052 5160 1678 Size: 374 3108 2531 Fragments arranged by size: 3108 2531 374 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2178 2210 4654 4686 4728 Size: 32 135 32 606 32 2444 32 42 Cuts at: 4728 4760 1373 Size: 32 2626 Fragments arranged by size: 2626 2444 606 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3357 3603 669 Size: 988 281 1419 246 3079 Fragments arranged by size: 3079 1419 988 281 246 HindIII A'AGCT_T Cuts at: 748 2818 3154 748 Size: 2070 336 3607 Fragments arranged by size: 3607 2070 336 HpaI GTT'AAC Cuts at: 3603 3603 Size: 6013 KpnI G_GTAC'C Cuts at: 3338 3338 Size: 6013 MfeI C'AATT_G Cuts at: 3612 3612 Size: 6013 MluI A'CGCG_T Cuts at: 5889 5889 Size: 6013 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2357 5127 5311 1305 Size: 1052 2770 184 2007 Fragments arranged by size: 2770 2007 1052 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4191 Fragments arranged by size: 4191 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2427 2511 2595 2763 2874 2931 3015 Size: 648 1261 84 84 168 111 57 84 Cuts at: 3015 3099 3971 4330 4489 518 Size: 84 872 359 159 2042 Fragments arranged by size: 2042 1261 872 648 359 168 159 111 84 84 84 84 57 MspA1I CmG'CkG Cuts at: 1606 3429 4106 5254 5499 1606 Size: 1823 677 1148 245 2120 Fragments arranged by size: 2120 1823 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4522 Fragments arranged by size: 4522 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6013 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6013 NotI GC'GGCC_GC Cuts at: 3460 3460 Size: 6013 NsiI A_TGCA'T Cuts at: 3038 3038 Size: 6013 NspI r_CATG'y Cuts at: 1132 1229 2140 3371 4916 1132 Size: 97 911 1231 1545 2229 Fragments arranged by size: 2229 1545 1231 911 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6013 PciI A'CATG_T Cuts at: 4912 4912 Size: 6013 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6013 PmeI GTTT'AAAC Cuts at: 3317 3317 Size: 6013 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4082 4114 5595 5627 4082 Size: 32 1481 32 4468 Fragments arranged by size: 4468 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6013 PsiI TTA'TAA Cuts at: 3583 3583 Size: 6013 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2475 2507 2475 Size: 32 5981 PstI C_TGCA'G Cuts at: 830 3365 830 Size: 2535 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6013 SalI G'TCGA_C Cuts at: 1936 3355 1936 Size: 1419 4594 SbfI CC_TGCA'GG Cuts at: 3365 3365 Size: 6013 ScaI AGT'ACT Cuts at: 4261 5973 4261 Size: 1712 4301 SfcI C'TryA_G Cuts at: 826 1045 3361 4496 5177 5368 826 Size: 219 2316 1135 681 191 1471 Fragments arranged by size: 2316 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6013 SmaI CCC'GGG Cuts at: 1872 3340 1872 Size: 1468 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2169 2815 4117 5018 5280 820 Size: 197 926 226 646 1302 901 262 1553 Fragments arranged by size: 1553 1302 926 901 646 262 226 197 SnaBI TAC'GTA Cuts at: 493 3804 493 Size: 3311 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6013 SphI G_CATG'C Cuts at: 1132 3371 1132 Size: 2239 3774 SspI AAT'ATT Cuts at: 5 52 3937 5 Size: 47 3885 2081 Fragments arranged by size: 3885 2081 47 StuI AGG'CCT Cuts at: 1517 2384 2465 2549 2801 3053 3137 1517 Size: 867 81 84 252 252 84 4393 Fragments arranged by size: 4393 867 252 252 84 84 81 StyI C'CwwG_G Cuts at: 513 1065 2004 3452 513 Size: 552 939 1448 3074 Fragments arranged by size: 3074 1448 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4040 4057 4210 4395 4840 325 Size: 1255 2460 17 153 185 445 1498 Fragments arranged by size: 2460 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4259 5971 96 Size: 274 80 33 51 1443 2282 1712 138 Fragments arranged by size: 2282 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5973 6005 5973 Size: 32 5981 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6013 XbaI T'CTAG_A Cuts at: 3349 3349 Size: 6013 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6013 XmaI C'CCGG_G Cuts at: 1870 3338 1870 Size: 1468 4545 XmnI GAAnn'nnTTC Cuts at: 2263 3137 3178 4142 2263 Size: 874 41 964 4134 Fragments arranged by size: 4134 964 874 41 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3821 276 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI