Restriction map for
pFN21AB4139
(Linear) (Six-base) MAPSORT of: KIBB4139.seq Check: 9047 from: 1 to: 1308
KIBB4139.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 668 1308
Size: 668 640
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 1119 1308
Size: 204 915 189
Fragments arranged by size:
915 204 189
AfeI AGC'GCT
Cuts at: 0 45 1308
Size: 45 1263
AleI CACnn'nnGTG
Cuts at: 0 506 1010 1308
Size: 506 504 298
Fragments arranged by size:
506 504 298
ApoI r'AATT_y
Cuts at: 0 7 169 413 497 749 833 1001 1085
Size: 7 162 244 84 252 84 168 84
Cuts at: 1085 1169 1308
Size: 84 139
Fragments arranged by size:
252 244 168 162 139 84 84 84
84 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 967 1308
Size: 967 341
BfrBI ATG'CAT
Cuts at: 0 1031 1308
Size: 1031 277
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 207 1308
Size: 207 1101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 1308
Size: 695 613
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 795 1308
Size: 149 646 513
Fragments arranged by size:
646 513 149
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 1270 1308
Size: 106 1164 38
Fragments arranged by size:
1164 106 38
BsmI GAATG_Cn'
Cuts at: 0 292 1033 1308
Size: 292 741 275
Fragments arranged by size:
741 292 275
BspHI T'CATG_A
Cuts at: 0 864 1023 1308
Size: 864 159 285
Fragments arranged by size:
864 285 159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 668 1308
Size: 668 640
BsrDI GCAATG_nn'
Cuts at: 0 360 387 622 1308
Size: 360 27 235 686
Fragments arranged by size:
686 360 235 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 706 1308
Size: 706 602
DraI TTT'AAA
Cuts at: 0 12 321 1308
Size: 12 309 987
Fragments arranged by size:
987 309 12
EarI CTCTTCn'nnn_
Cuts at: 0 1250 1308
Size: 1250 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 695 1119 1308
Size: 204 491 424 189
Fragments arranged by size:
491 424 204 189
EcoRI G'AATT_C
Cuts at: 0 413 497 749 1001 1085 1169 1308
Size: 413 84 252 252 84 84 139
Fragments arranged by size:
413 252 252 139 84 84 84
HaeII r_GCGC'y
Cuts at: 0 47 1308
Size: 47 1261
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 173 205 1308
Size: 173 32 1103
Fragments arranged by size:
1103 173 32
HindIII A'AGCT_T
Cuts at: 0 813 1149 1308
Size: 813 336 159
Fragments arranged by size:
813 336 159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 1308
Size: 352 956
MslI CAynn'nnrTG
Cuts at: 0 422 506 590 758 869 926 1010 1094
Size: 422 84 84 168 111 57 84 84
Cuts at: 1094 1308
Size: 214
Fragments arranged by size:
422 214 168 111 84 84 84 84
57
NsiI A_TGCA'T
Cuts at: 0 1033 1308
Size: 1033 275
NspI r_CATG'y
Cuts at: 0 135 1308
Size: 135 1173
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 470 502 1308
Size: 470 32 806
Fragments arranged by size:
806 470 32
SmlI C'TyrA_G
Cuts at: 0 164 810 1308
Size: 164 646 498
Fragments arranged by size:
646 498 164
StuI AGG'CCT
Cuts at: 0 379 460 544 796 1048 1132 1308
Size: 379 81 84 252 252 84 176
Fragments arranged by size:
379 252 252 176 84 84 81
XmnI GAAnn'nnTTC
Cuts at: 0 258 1132 1173 1308
Size: 258 874 41 135
Fragments arranged by size:
874 258 135 41
Enzymes that do cut:
AarI AcuI AfeI AleI ApoI BbsI BfrBI BplI
BpmI BpuEI BseRI BsmI BspHI BspMI BsrDI BtgZI
DraI EarI Eco57MI EcoRI HaeII Hin4I HindIII MmeI
MslI NsiI NspI PsrI SmlI StuI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI ClaI
CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI MscI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI