(Circular) (Six-base) MAPSORT of: pFN21AB4122.seq Check: 9286 from: 1 to: 6547 pFN21AB4122 6547 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4357 278 Size: 53 83 186 936 2821 2468 Fragments arranged by size: 2821 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2597 3664 3890 1937 Size: 660 1067 226 4594 Fragments arranged by size: 4594 1067 660 226 Acc65I G'GTAC_C Cuts at: 3868 3868 Size: 6547 AclI AA'CG_TT Cuts at: 1563 4674 5047 1563 Size: 3111 373 3063 Fragments arranged by size: 3111 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2895 2930 3404 4610 5994 6262 1632 Size: 1263 35 474 1206 1384 268 1917 Fragments arranged by size: 1917 1384 1263 1206 474 268 35 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6350 AflIII A'CryG_T Cuts at: 1203 5446 6423 1203 Size: 4243 977 1327 Fragments arranged by size: 4243 1327 977 AgeI A'CCGG_T Cuts at: 5378 5378 Size: 6547 AhdI GACnn_n'nnGTC Cuts at: 5276 5276 Size: 6547 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6547 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2580 1184 Size: 1396 5151 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3729 3761 3729 Size: 32 6515 AlwNI CAG_nnn'CTG Cuts at: 4290 5862 4290 Size: 1572 4975 ApaI G_GGCC'C Cuts at: 2088 2088 Size: 6547 ApaLI G'TGCA_C Cuts at: 2055 2913 4604 5760 2055 Size: 858 1691 1156 2842 Fragments arranged by size: 2842 1691 1156 858 ApoI r'AATT_y Cuts at: 1493 2162 2173 2287 2341 3161 3236 3856 4082 Size: 669 11 114 54 820 75 620 226 Cuts at: 4082 4234 6343 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 820 669 620 226 152 114 75 54 11 AseI AT'TA_AT Cuts at: 160 1032 3405 5101 160 Size: 872 2373 1696 1606 Fragments arranged by size: 2373 1696 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 AvaI C'yCGr_G Cuts at: 1870 1943 2532 3872 1870 Size: 73 589 1340 4545 Fragments arranged by size: 4545 1340 589 73 BamHI G'GATC_C Cuts at: 3877 4244 3877 Size: 367 6180 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2524 3868 5323 618 Size: 325 217 627 737 1344 1455 1842 Fragments arranged by size: 1842 1455 1344 737 627 325 217 BanII G_rGCy'C Cuts at: 1391 2088 3866 1391 Size: 697 1778 4072 Fragments arranged by size: 4072 1778 697 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2689 3438 928 Size: 971 790 749 4037 Fragments arranged by size: 4037 971 790 749 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4738 4772 1903 Size: 34 2801 34 3678 Fragments arranged by size: 3678 2801 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3125 4439 5649 3125 Size: 1314 1210 4023 Fragments arranged by size: 4023 1314 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6547 BfrBI ATG'CAT Cuts at: 3785 3785 Size: 6547 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5158 136 Size: 107 122 71 631 605 3486 1525 Fragments arranged by size: 3486 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6542 6542 Size: 6547 BlpI GC'TnA_GC Cuts at: 3964 3964 Size: 6547 Bme1580I G_kGCm'C Cuts at: 1790 2059 2088 2917 4608 5764 1790 Size: 269 29 829 1691 1156 2573 Fragments arranged by size: 2573 1691 1156 829 269 29 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6547 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3157 4278 5236 Size: 416 53 83 399 24 1740 1121 958 Cuts at: 5236 442 Size: 1753 Fragments arranged by size: 1753 1740 1121 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6547 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3193 5207 1239 Size: 1954 2014 2579 Fragments arranged by size: 2579 2014 1954 Bpu10I CC'TnA_GC Cuts at: 2370 2370 Size: 6547 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4672 5537 5835 4672 Size: 865 298 5384 Fragments arranged by size: 5384 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2022 5210 882 Size: 617 523 3188 2219 Fragments arranged by size: 3188 2219 617 523 BsaAI yAC'GTr Cuts at: 493 1204 2639 4338 493 Size: 711 1435 1699 2702 Fragments arranged by size: 2702 1699 1435 711 BsaBI GATnn'nnATC Cuts at: 6541 6541 Size: 6547 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4354 4736 5371 275 Size: 53 83 186 936 2821 382 635 1451 Fragments arranged by size: 2821 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4980 5378 5652 5799 6210 4980 Size: 398 274 147 411 5317 Fragments arranged by size: 5317 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3248 3278 1178 Size: 30 165 30 1845 30 4447 Fragments arranged by size: 4447 1845 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3103 3256 3477 1186 Size: 1917 153 221 4256 Fragments arranged by size: 4256 1917 221 153 BseYI C'CCAG_C Cuts at: 5750 6246 5750 Size: 496 6051 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2573 3789 1163 Size: 573 837 1216 3921 Fragments arranged by size: 3921 1216 837 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2038 3997 4758 5786 1540 Size: 275 186 37 1959 761 1028 2301 Fragments arranged by size: 2301 1959 1028 761 275 186 37 BsiHKAI G_wGCw'C Cuts at: 2059 2190 2620 2917 4608 4693 5764 2059 Size: 131 430 297 1691 85 1071 2842 Fragments arranged by size: 2842 1691 1071 430 297 131 85 BsmI GAATG_Cn' Cuts at: 2766 4058 4151 2766 Size: 1292 93 5162 Fragments arranged by size: 5162 1292 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2059 2088 2190 2620 2917 3866 4608 Size: 399 269 29 102 430 297 949 742 Cuts at: 4608 4693 5764 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 949 742 430 399 297 269 102 85 29 BspEI T'CCGG_A Cuts at: 6210 6210 Size: 6547 BspHI T'CATG_A Cuts at: 2965 3012 4436 6166 2965 Size: 47 1424 1730 3346 Fragments arranged by size: 3346 1730 1424 47 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3902 844 Size: 3058 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4434 1394 Size: 429 2611 3507 Fragments arranged by size: 3507 2611 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5042 5216 65 Size: 1183 3794 174 1396 Fragments arranged by size: 3794 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5191 5378 1700 Size: 110 3381 187 2869 Fragments arranged by size: 3381 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6547 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6547 BssSI C'ACGA_G Cuts at: 4607 5619 4607 Size: 1012 5535 BstAPI GCAn_nnn'nTGC Cuts at: 794 3791 794 Size: 2997 3550 BstBI TT'CG_AA Cuts at: 5351 5351 Size: 6547 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6547 BstYI r'GATC_y Cuts at: 1972 2426 3877 4244 4629 4646 6098 6184 6196 Size: 454 1451 367 385 17 1452 86 12 Cuts at: 6196 6542 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1451 454 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3665 3665 Size: 6547 Bsu36I CC'TnA_GG Cuts at: 2622 2622 Size: 6547 BtgI C'CryG_G Cuts at: 513 1065 2032 3108 3118 513 Size: 552 967 1076 10 3942 Fragments arranged by size: 3942 1076 967 552 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2514 487 Size: 683 56 1288 4520 Fragments arranged by size: 4520 1288 683 56 BtsI GCAGTG_nn' Cuts at: 2355 4063 4857 4877 2355 Size: 1708 794 20 4025 Fragments arranged by size: 4025 1708 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6547 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3493 3528 529 Size: 35 2929 35 3548 Fragments arranged by size: 3548 2929 35 35 DraI TTT'AAA Cuts at: 3851 3851 Size: 6547 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5554 809 Size: 507 4238 1802 Fragments arranged by size: 4238 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2035 2378 2849 Size: 54 1425 50 275 18 205 343 471 Cuts at: 2849 3994 4883 6512 8 Size: 1145 889 1629 43 Fragments arranged by size: 1629 1425 1145 889 471 343 275 205 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2035 3994 1537 Size: 275 223 1959 4090 Fragments arranged by size: 4090 1959 275 223 EarI CTCTTCn'nnn_ Cuts at: 1353 3000 4477 1353 Size: 1647 1477 3423 Fragments arranged by size: 3423 1647 1477 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5134 5508 5654 387 Size: 1064 138 3545 374 146 1280 Fragments arranged by size: 3545 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2895 2930 3193 3404 4610 5207 5994 Size: 393 1263 35 263 211 1206 597 787 Cuts at: 5994 6262 1239 Size: 268 1524 Fragments arranged by size: 1524 1263 1206 787 597 393 268 263 211 35 EcoO109I rG'GnC_Cy Cuts at: 2084 2085 2084 Size: 1 6546 EcoRI G'AATT_C Cuts at: 3161 3856 4234 3161 Size: 695 378 5474 Fragments arranged by size: 5474 695 378 FspI TGC'GCA Cuts at: 5053 5053 Size: 6547 HaeII r_GCGC'y Cuts at: 1678 5694 1678 Size: 4016 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2788 2820 3191 3223 3359 Size: 32 135 32 1216 32 371 32 136 Cuts at: 3359 3391 3479 3511 3673 3705 3805 3837 5188 Size: 32 88 32 162 32 100 32 1351 Cuts at: 5188 5220 5262 5294 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1351 1216 371 162 136 135 100 88 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3134 3891 4137 669 Size: 988 281 1196 757 246 3079 Fragments arranged by size: 3079 1196 988 757 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6547 HpaI GTT'AAC Cuts at: 4137 4137 Size: 6547 KpnI G_GTAC'C Cuts at: 3872 3872 Size: 6547 MfeI C'AATT_G Cuts at: 4146 4146 Size: 6547 MluI A'CGCG_T Cuts at: 6423 6423 Size: 6547 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2389 3716 5661 5845 1305 Size: 1084 1327 1945 184 2007 Fragments arranged by size: 2007 1945 1327 1084 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2380 2851 10 Size: 54 1425 343 548 471 3706 Fragments arranged by size: 3706 1425 548 471 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4505 4864 5023 518 Size: 648 3339 359 159 2042 Fragments arranged by size: 3339 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2034 3803 3963 4640 5788 6033 1606 Size: 428 1769 160 677 1148 245 2120 Fragments arranged by size: 2120 1769 1148 677 428 245 160 NcoI C'CATG_G Cuts at: 513 1065 3108 3118 513 Size: 552 2043 10 3942 Fragments arranged by size: 3942 2043 552 10 NdeI CA'TA_TG Cuts at: 387 3781 387 Size: 3394 3153 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6547 NotI GC'GGCC_GC Cuts at: 3994 3994 Size: 6547 NsiI A_TGCA'T Cuts at: 3787 3787 Size: 6547 NspI r_CATG'y Cuts at: 1132 1229 2586 3905 5450 1132 Size: 97 1357 1319 1545 2229 Fragments arranged by size: 2229 1545 1357 1319 97 PasI CC'CwG_GG Cuts at: 1348 2114 1348 Size: 766 5781 PciI A'CATG_T Cuts at: 5446 5446 Size: 6547 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6547 PmeI GTTT'AAAC Cuts at: 3851 3851 Size: 6547 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4616 4648 6129 6161 4616 Size: 32 1481 32 5002 Fragments arranged by size: 5002 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3129 1613 Size: 1516 5031 PsiI TTA'TAA Cuts at: 4117 4117 Size: 6547 PspOMI G'GGCC_C Cuts at: 2084 2084 Size: 6547 PstI C_TGCA'G Cuts at: 830 3802 3899 830 Size: 2972 97 3478 Fragments arranged by size: 3478 2972 97 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6547 PvuII CAG'CTG Cuts at: 3803 3803 Size: 6547 SacII CC_GC'GG Cuts at: 2035 2035 Size: 6547 SalI G'TCGA_C Cuts at: 1936 3889 1936 Size: 1953 4594 SbfI CC_TGCA'GG Cuts at: 3899 3899 Size: 6547 ScaI AGT'ACT Cuts at: 4795 6507 4795 Size: 1712 4835 SfcI C'TryA_G Cuts at: 826 1045 3195 3798 3895 5030 5711 5902 826 Size: 219 2150 603 97 1135 681 191 1471 Fragments arranged by size: 2150 1471 1135 681 603 219 191 97 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6547 SmaI CCC'GGG Cuts at: 1872 3874 1872 Size: 2002 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4651 5552 5814 820 Size: 197 926 2708 901 262 1553 Fragments arranged by size: 2708 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4338 493 Size: 3845 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6547 SphI G_CATG'C Cuts at: 1132 3905 1132 Size: 2773 3774 SspI AAT'ATT Cuts at: 5 52 2573 4471 5 Size: 47 2521 1898 2081 Fragments arranged by size: 2521 2081 1898 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6547 StyI C'CwwG_G Cuts at: 513 1065 3108 3118 3986 513 Size: 552 2043 10 868 3074 Fragments arranged by size: 3074 2043 868 552 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2040 2058 3087 4574 4591 4744 4929 Size: 1255 460 18 1029 1487 17 153 185 Cuts at: 4929 5374 325 Size: 445 1498 Fragments arranged by size: 1498 1487 1255 1029 460 445 185 153 18 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4793 6505 96 Size: 274 80 33 51 1443 2816 1712 138 Fragments arranged by size: 2816 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6507 6539 6507 Size: 32 6515 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6547 XbaI T'CTAG_A Cuts at: 3883 3883 Size: 6547 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3043 3043 Size: 6547 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6547 XmaI C'CCGG_G Cuts at: 1870 3872 1870 Size: 2002 4545 XmnI GAAnn'nnTTC Cuts at: 4676 4676 Size: 6547 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4355 276 Size: 53 83 186 936 2821 2468 Fragments arranged by size: 2821 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI