(Circular) (Six-base) MAPSORT of: pFN21AB4108.seq Check: 2547 from: 1 to: 7636 pFN21AB4108 7636 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5446 278 Size: 53 83 186 936 3910 2468 Fragments arranged by size: 3910 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4731 4979 1937 Size: 2794 248 4594 Fragments arranged by size: 4594 2794 248 Acc65I G'GTAC_C Cuts at: 4005 4957 4005 Size: 952 6684 AclI AA'CG_TT Cuts at: 1563 3692 5763 6136 1563 Size: 2129 2071 373 3063 Fragments arranged by size: 3063 2129 2071 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2669 3266 3448 4206 5699 7083 7351 1632 Size: 1037 597 182 758 1493 1384 268 1917 Fragments arranged by size: 1917 1493 1384 1037 758 597 268 182 AflII C'TTAA_G Cuts at: 820 1017 2272 820 Size: 197 1255 6184 Fragments arranged by size: 6184 1255 197 AflIII A'CryG_T Cuts at: 1203 2999 3411 3846 6535 7512 1203 Size: 1796 412 435 2689 977 1327 Fragments arranged by size: 2689 1796 1327 977 435 412 AgeI A'CCGG_T Cuts at: 6467 6467 Size: 7636 AhdI GACnn_n'nnGTC Cuts at: 6365 6365 Size: 7636 AleI CACnn'nnGTG Cuts at: 1166 2743 3428 4033 1166 Size: 1577 685 605 4769 Fragments arranged by size: 4769 1577 685 605 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3696 1184 Size: 2512 5124 AlwNI CAG_nnn'CTG Cuts at: 2692 2932 3715 5379 6951 2692 Size: 240 783 1664 1572 3377 Fragments arranged by size: 3377 1664 1572 783 240 ApaLI G'TGCA_C Cuts at: 5693 6849 5693 Size: 1156 6480 ApoI r'AATT_y Cuts at: 1493 2172 2417 2711 3499 3776 3911 4321 4381 Size: 679 245 294 788 277 135 410 60 Cuts at: 4381 4513 4945 5171 5323 7432 1493 Size: 132 432 226 152 2109 1697 Fragments arranged by size: 2109 1697 788 679 432 410 294 277 245 226 152 135 132 60 AseI AT'TA_AT Cuts at: 160 1032 2193 6190 160 Size: 872 1161 3997 1606 Fragments arranged by size: 3997 1606 1161 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7636 AvaI C'yCGr_G Cuts at: 1870 1943 3985 4668 4961 1870 Size: 73 2042 683 293 4545 Fragments arranged by size: 4545 2042 683 293 73 BamHI G'GATC_C Cuts at: 4966 5333 4966 Size: 367 7269 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2383 3794 4005 4957 6412 Size: 325 217 627 596 1411 211 952 1455 Cuts at: 6412 618 Size: 1842 Fragments arranged by size: 1842 1455 1411 952 627 596 325 217 211 BanII G_rGCy'C Cuts at: 1391 2259 3738 3992 4955 1391 Size: 868 1479 254 963 4072 Fragments arranged by size: 4072 1479 963 868 254 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2980 3022 4114 4308 928 Size: 971 1081 42 1092 194 4256 Fragments arranged by size: 4256 1092 1081 971 194 42 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5827 5861 1903 Size: 34 3890 34 3678 Fragments arranged by size: 3890 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5528 6738 5528 Size: 1210 6426 BclI T'GATC_A Cuts at: 1548 4011 1548 Size: 2463 5173 BfrBI ATG'CAT Cuts at: 3683 3683 Size: 7636 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6247 136 Size: 107 122 71 631 605 4575 1525 Fragments arranged by size: 4575 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7631 7631 Size: 7636 BlpI GC'TnA_GC Cuts at: 4575 5053 4575 Size: 478 7158 Bme1580I G_kGCm'C Cuts at: 1790 3388 5697 6853 1790 Size: 1598 2309 1156 2573 Fragments arranged by size: 2573 2309 1598 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7636 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2989 4769 5367 Size: 416 53 83 399 24 1572 1780 598 Cuts at: 5367 6325 442 Size: 958 1753 Fragments arranged by size: 1780 1753 1572 958 598 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3590 1056 Size: 2534 5102 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2409 2572 2986 6296 1239 Size: 1170 163 414 3310 2579 Fragments arranged by size: 3310 2579 1170 414 163 Bpu10I CC'TnA_GC Cuts at: 2760 4694 2760 Size: 1934 5702 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4123 4664 5761 6626 6924 4123 Size: 541 1097 865 298 4835 Fragments arranged by size: 4835 1097 865 541 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2043 4712 6299 882 Size: 617 544 2669 1587 2219 Fragments arranged by size: 2669 2219 1587 617 544 BsaAI yAC'GTr Cuts at: 493 1204 4921 5427 493 Size: 711 3717 506 2702 Fragments arranged by size: 3717 2702 711 506 BsaBI GATnn'nnATC Cuts at: 7630 7630 Size: 7636 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5443 5825 6460 275 Size: 53 83 186 936 3910 382 635 1451 Fragments arranged by size: 3910 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6069 6467 6741 6888 7299 6069 Size: 398 274 147 411 6406 Fragments arranged by size: 6406 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2490 2520 2570 2600 3101 Size: 30 165 30 1087 30 50 30 501 Cuts at: 3101 3131 1178 Size: 30 5683 Fragments arranged by size: 5683 1087 501 165 50 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2216 2983 1186 Size: 1030 767 5839 Fragments arranged by size: 5839 1030 767 BseYI C'CCAG_C Cuts at: 2910 6839 7335 2910 Size: 3929 496 3211 Fragments arranged by size: 3929 3211 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7063 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5086 5847 6875 1540 Size: 275 186 3085 761 1028 2301 Fragments arranged by size: 3085 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2259 5697 5782 6853 2259 Size: 3438 85 1071 3042 Fragments arranged by size: 3438 3042 1071 85 BsmI GAATG_Cn' Cuts at: 2122 2549 3543 4306 4698 5147 5240 2122 Size: 427 994 763 392 449 93 4518 Fragments arranged by size: 4518 994 763 449 427 392 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2259 3388 3738 3992 4955 5697 5782 Size: 399 469 1129 350 254 963 742 85 Cuts at: 5782 6853 1391 Size: 1071 2174 Fragments arranged by size: 2174 1129 1071 963 742 469 399 350 254 85 BspEI T'CCGG_A Cuts at: 7299 7299 Size: 7636 BspHI T'CATG_A Cuts at: 5525 7255 5525 Size: 1730 5906 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3656 4991 844 Size: 2812 1335 3489 Fragments arranged by size: 3489 2812 1335 BsrBI CCG'CTC Cuts at: 1394 1823 5523 1394 Size: 429 3700 3507 Fragments arranged by size: 3700 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2229 2628 4170 4465 6131 6305 65 Size: 1183 981 399 1542 295 1666 174 1396 Fragments arranged by size: 1666 1542 1396 1183 981 399 295 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6280 6467 1700 Size: 110 4470 187 2869 Fragments arranged by size: 4470 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7636 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7636 BssSI C'ACGA_G Cuts at: 2519 3396 5696 6708 2519 Size: 877 2300 1012 3447 Fragments arranged by size: 3447 2300 1012 877 BstAPI GCAn_nnn'nTGC Cuts at: 794 4433 794 Size: 3639 3997 BstBI TT'CG_AA Cuts at: 6440 6440 Size: 7636 BstEII G'GTnAC_C Cuts at: 1184 4688 1184 Size: 3504 4132 BstXI CCAn_nnnn'nTGG Cuts at: 2797 3430 4933 2797 Size: 633 1503 5500 Fragments arranged by size: 5500 1503 633 BstYI r'GATC_y Cuts at: 1972 2647 4020 4966 5333 5718 5735 7187 7273 Size: 675 1373 946 367 385 17 1452 86 Cuts at: 7273 7285 7631 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1373 946 675 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3227 513 Size: 552 939 1223 4922 Fragments arranged by size: 4922 1223 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6897 Fragments arranged by size: 6897 683 56 BtsI GCAGTG_nn' Cuts at: 2750 2991 3704 5152 5946 5966 2750 Size: 241 713 1448 794 20 4420 Fragments arranged by size: 4420 1448 794 713 241 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7636 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3787 3822 529 Size: 35 3223 35 4343 Fragments arranged by size: 4343 3223 35 35 DraI TTT'AAA Cuts at: 2182 4940 2182 Size: 2758 4878 DraIII CAC_nnn'GTG Cuts at: 4688 4688 Size: 7636 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3709 6643 809 Size: 507 2393 2934 1802 Fragments arranged by size: 2934 2393 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2613 2877 5083 Size: 54 1425 50 275 18 783 264 2206 Cuts at: 5083 5972 7601 8 Size: 889 1629 43 Fragments arranged by size: 2206 1629 1425 889 783 275 264 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5083 1537 Size: 275 3271 4090 Fragments arranged by size: 4090 3271 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2724 4197 4854 5566 1353 Size: 1371 1473 657 712 3423 Fragments arranged by size: 3423 1473 1371 712 657 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3291 6223 6597 6743 387 Size: 1064 138 1702 2932 374 146 1280 Fragments arranged by size: 2932 1702 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2257 2257 Size: 7636 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2409 2572 2669 2986 3266 3448 4206 Size: 393 777 163 97 317 280 182 758 Cuts at: 4206 5699 6296 7083 7351 1239 Size: 1493 597 787 268 1524 Fragments arranged by size: 1524 1493 787 777 758 597 393 317 280 268 182 163 97 EcoO109I rG'GnC_Cy Cuts at: 2764 3232 3512 2764 Size: 468 280 6888 Fragments arranged by size: 6888 468 280 EcoRI G'AATT_C Cuts at: 2417 2711 4945 5323 2417 Size: 294 2234 378 4730 Fragments arranged by size: 4730 2234 378 294 EcoRV GAT'ATC Cuts at: 4528 4528 Size: 7636 FspI TGC'GCA Cuts at: 6142 6142 Size: 7636 HaeII r_GCGC'y Cuts at: 1678 6783 1678 Size: 5105 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2040 2072 2583 2615 3063 Size: 32 135 32 468 32 511 32 448 Cuts at: 3063 3095 4096 4128 4771 4803 6277 6309 6351 Size: 32 1001 32 643 32 1474 32 42 Cuts at: 6351 6383 1373 Size: 32 2626 Fragments arranged by size: 2626 1474 1001 643 511 468 448 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2924 3645 4625 4980 5226 669 Size: 988 281 986 721 980 355 246 3079 Fragments arranged by size: 3079 988 986 980 721 355 281 246 HindIII A'AGCT_T Cuts at: 748 2275 3926 748 Size: 1527 1651 4458 Fragments arranged by size: 4458 1651 1527 HpaI GTT'AAC Cuts at: 3645 5226 3645 Size: 1581 6055 KpnI G_GTAC'C Cuts at: 4009 4961 4009 Size: 952 6684 MfeI C'AATT_G Cuts at: 2235 2917 3611 4431 5235 2235 Size: 682 694 820 804 4636 Fragments arranged by size: 4636 820 804 694 682 MluI A'CGCG_T Cuts at: 7512 7512 Size: 7636 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2272 2311 2700 6750 6934 1305 Size: 967 39 389 4050 184 2007 Fragments arranged by size: 4050 2007 967 389 184 39 MscI TGG'CCA Cuts at: 10 64 1489 1832 2615 2879 10 Size: 54 1425 343 783 264 4767 Fragments arranged by size: 4767 1425 783 343 264 54 MslI CAynn'nnrTG Cuts at: 518 1166 2743 3428 4033 5594 5953 6112 518 Size: 648 1577 685 605 1561 359 159 2042 Fragments arranged by size: 2042 1577 1561 685 648 605 359 159 MspA1I CmG'CkG Cuts at: 1606 2529 2929 5052 5729 6877 7122 1606 Size: 923 400 2123 677 1148 245 2120 Fragments arranged by size: 2123 2120 1148 923 677 400 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6145 Fragments arranged by size: 6145 939 552 NdeI CA'TA_TG Cuts at: 387 3245 387 Size: 2858 4778 NheI G'CTAG_C Cuts at: 1052 3586 1052 Size: 2534 5102 NotI GC'GGCC_GC Cuts at: 5083 5083 Size: 7636 NsiI A_TGCA'T Cuts at: 3685 3685 Size: 7636 NspI r_CATG'y Cuts at: 1132 1229 2312 3003 3415 3850 4853 4994 6539 Size: 97 1083 691 412 435 1003 141 1545 Cuts at: 6539 1132 Size: 2229 Fragments arranged by size: 2229 1545 1083 1003 691 435 412 141 97 PasI CC'CwG_GG Cuts at: 1348 4491 1348 Size: 3143 4493 PciI A'CATG_T Cuts at: 2999 3411 3846 6535 2999 Size: 412 435 2689 4100 Fragments arranged by size: 4100 2689 435 412 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7636 PfoI T'CCnGG_A Cuts at: 3543 3543 Size: 7636 PmeI GTTT'AAAC Cuts at: 4940 4940 Size: 7636 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5705 5737 7218 7250 5705 Size: 32 1481 32 6091 Fragments arranged by size: 6091 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2764 3232 3512 2764 Size: 468 280 6888 Fragments arranged by size: 6888 468 280 PshAI GACnn'nnGTC Cuts at: 1613 2829 3758 1613 Size: 1216 929 5491 Fragments arranged by size: 5491 1216 929 PsiI TTA'TAA Cuts at: 5206 5206 Size: 7636 PstI C_TGCA'G Cuts at: 830 3711 4636 4988 830 Size: 2881 925 352 3478 Fragments arranged by size: 3478 2881 925 352 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7636 PvuII CAG'CTG Cuts at: 2929 2929 Size: 7636 SacI G_AGCT'C Cuts at: 2259 2259 Size: 7636 SalI G'TCGA_C Cuts at: 1936 4978 1936 Size: 3042 4594 SapI GCTCTTCn'nnn_ Cuts at: 2724 2724 Size: 7636 SbfI CC_TGCA'GG Cuts at: 4988 4988 Size: 7636 ScaI AGT'ACT Cuts at: 5884 7596 5884 Size: 1712 5924 SexAI A'CCwGG_T Cuts at: 4140 4140 Size: 7636 SfcI C'TryA_G Cuts at: 826 1045 2044 3707 4632 4984 6119 6800 6991 Size: 219 999 1663 925 352 1135 681 191 Cuts at: 6991 826 Size: 1471 Fragments arranged by size: 1663 1471 1135 999 925 681 352 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7636 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7636 SmaI CCC'GGG Cuts at: 1872 4963 1872 Size: 3091 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2272 4102 4643 4668 5740 6641 Size: 197 926 329 1830 541 25 1072 901 Cuts at: 6641 6903 820 Size: 262 1553 Fragments arranged by size: 1830 1553 1072 926 901 541 329 262 197 25 SnaBI TAC'GTA Cuts at: 493 5427 493 Size: 4934 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7636 SphI G_CATG'C Cuts at: 1132 4994 1132 Size: 3862 3774 SspI AAT'ATT Cuts at: 5 52 2191 3729 4654 5560 5 Size: 47 2139 1538 925 906 2081 Fragments arranged by size: 2139 2081 1538 925 906 47 StuI AGG'CCT Cuts at: 1517 2609 4194 1517 Size: 1092 1585 4959 Fragments arranged by size: 4959 1585 1092 StyI C'CwwG_G Cuts at: 513 1065 2004 2610 5075 513 Size: 552 939 606 2465 3074 Fragments arranged by size: 3074 2465 939 606 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4214 4538 4922 5663 5680 5833 6018 Size: 1255 2634 324 384 741 17 153 185 Cuts at: 6018 6463 325 Size: 445 1498 Fragments arranged by size: 2634 1498 1255 741 445 384 324 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3378 3615 5882 Size: 274 80 33 51 1443 1401 237 2267 Cuts at: 5882 7594 96 Size: 1712 138 Fragments arranged by size: 2267 1712 1443 1401 274 237 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2490 2522 2570 2602 3101 3133 4767 4799 7596 Size: 32 48 32 499 32 1634 32 2797 Cuts at: 7596 7628 2490 Size: 32 2498 Fragments arranged by size: 2797 2498 1634 499 48 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4901 1316 Size: 3585 4051 XbaI T'CTAG_A Cuts at: 4972 4972 Size: 7636 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2560 3402 2560 Size: 842 6794 XhoI C'TCGA_G Cuts at: 1943 4668 1943 Size: 2725 4911 XmaI C'CCGG_G Cuts at: 1870 4961 1870 Size: 3091 4545 XmnI GAAnn'nnTTC Cuts at: 2805 5765 2805 Size: 2960 4676 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5444 276 Size: 53 83 186 936 3910 2468 Fragments arranged by size: 3910 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI