(Circular) (Six-base) MAPSORT of: pFN21AB4092.seq Check: 3369 from: 1 to: 5950 pFN21AB4092 5950 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2117 2117 Size: 5950 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3760 278 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3293 1937 Size: 1356 4594 Acc65I G'GTAC_C Cuts at: 3271 3271 Size: 5950 AclI AA'CG_TT Cuts at: 1563 4077 4450 1563 Size: 2514 373 3063 Fragments arranged by size: 3063 2514 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2621 4013 5397 5665 1632 Size: 989 1392 1384 268 1917 Fragments arranged by size: 1917 1392 1384 989 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5753 AflIII A'CryG_T Cuts at: 1203 4849 5826 1203 Size: 3646 977 1327 Fragments arranged by size: 3646 1327 977 AgeI A'CCGG_T Cuts at: 2244 4781 2244 Size: 2537 3413 AhdI GACnn_n'nnGTC Cuts at: 4679 4679 Size: 5950 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5950 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5950 AlwNI CAG_nnn'CTG Cuts at: 2802 3693 5265 2802 Size: 891 1572 3487 Fragments arranged by size: 3487 1572 891 ApaI G_GGCC'C Cuts at: 2018 2631 2018 Size: 613 5337 ApaLI G'TGCA_C Cuts at: 4007 5163 4007 Size: 1156 4794 ApoI r'AATT_y Cuts at: 1493 3088 3259 3485 3637 5746 1493 Size: 1595 171 226 152 2109 1697 Fragments arranged by size: 2109 1697 1595 226 171 152 AseI AT'TA_AT Cuts at: 160 1032 4504 160 Size: 872 3472 1606 Fragments arranged by size: 3472 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 AvaI C'yCGr_G Cuts at: 1870 1943 2642 3275 1870 Size: 73 699 633 4545 Fragments arranged by size: 4545 699 633 73 BamHI G'GATC_C Cuts at: 3280 3647 3280 Size: 367 5583 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2705 3271 4726 618 Size: 325 217 627 918 566 1455 1842 Fragments arranged by size: 1842 1455 918 627 566 325 217 BanII G_rGCy'C Cuts at: 1391 2018 2631 3269 1391 Size: 627 613 638 4072 Fragments arranged by size: 4072 638 627 613 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4979 BbvCI CC'TCA_GC Cuts at: 2795 2795 Size: 5950 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4141 4175 1903 Size: 34 2204 34 3678 Fragments arranged by size: 3678 2204 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3842 5052 3842 Size: 1210 4740 BclI T'GATC_A Cuts at: 1548 1548 Size: 5950 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4561 136 Size: 107 122 71 631 605 2889 1525 Fragments arranged by size: 2889 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5945 5945 Size: 5950 BlpI GC'TnA_GC Cuts at: 3367 3367 Size: 5950 Bme1580I G_kGCm'C Cuts at: 1790 2018 2538 2631 4011 5167 1790 Size: 228 520 93 1380 1156 2573 Fragments arranged by size: 2573 1380 1156 520 228 93 BmgBI CAC'GTC Cuts at: 1318 2083 1318 Size: 765 5185 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3681 4639 442 Size: 416 53 83 399 24 2264 958 1753 Fragments arranged by size: 2264 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5950 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3200 4610 1239 Size: 1961 1410 2579 Fragments arranged by size: 2579 1961 1410 Bpu10I CC'TnA_GC Cuts at: 2795 2795 Size: 5950 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4075 4940 5238 4075 Size: 865 298 4787 Fragments arranged by size: 4787 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2784 4613 882 Size: 617 1285 1829 2219 Fragments arranged by size: 2219 1829 1285 617 BsaAI yAC'GTr Cuts at: 493 1204 3741 493 Size: 711 2537 2702 Fragments arranged by size: 2702 2537 711 BsaBI GATnn'nnATC Cuts at: 5944 5944 Size: 5950 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3757 4139 4774 275 Size: 53 83 186 936 2224 382 635 1451 Fragments arranged by size: 2224 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2244 2810 4383 4781 5055 5202 5613 2244 Size: 566 1573 398 274 147 411 2581 Fragments arranged by size: 2581 1573 566 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2792 2822 3173 3203 1178 Size: 30 165 30 1389 30 351 30 3925 Fragments arranged by size: 3925 1389 351 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2750 2925 1186 Size: 1564 175 4211 Fragments arranged by size: 4211 1564 175 BseYI C'CCAG_C Cuts at: 2011 5153 5649 2011 Size: 3142 496 2312 Fragments arranged by size: 3142 2312 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2089 2105 2495 2636 2873 1163 Size: 573 353 16 390 141 237 4240 Fragments arranged by size: 4240 573 390 353 237 141 16 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2907 3400 4161 5189 1540 Size: 275 186 906 493 761 1028 2301 Fragments arranged by size: 2301 1028 906 761 493 275 186 BsiHKAI G_wGCw'C Cuts at: 2861 4011 4096 5167 2861 Size: 1150 85 1071 3644 Fragments arranged by size: 3644 1150 1071 85 BsmI GAATG_Cn' Cuts at: 3461 3554 3461 Size: 93 5857 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2538 2631 2861 3269 4011 4096 Size: 399 228 520 93 230 408 742 85 Cuts at: 4096 5167 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 520 408 399 230 228 93 85 BspEI T'CCGG_A Cuts at: 2810 5613 2810 Size: 2803 3147 BspHI T'CATG_A Cuts at: 2113 3016 3839 5569 2113 Size: 903 823 1730 2494 Fragments arranged by size: 2494 1730 903 823 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2117 3305 844 Size: 1273 1188 3489 Fragments arranged by size: 3489 1273 1188 BsrBI CCG'CTC Cuts at: 1394 1823 3837 1394 Size: 429 2014 3507 Fragments arranged by size: 3507 2014 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2429 3115 4445 4619 65 Size: 1183 1181 686 1330 174 1396 Fragments arranged by size: 1396 1330 1183 1181 686 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2244 2637 3212 4594 4781 1700 Size: 110 434 393 575 1382 187 2869 Fragments arranged by size: 2869 1382 575 434 393 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5950 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5950 BssSI C'ACGA_G Cuts at: 4010 5022 4010 Size: 1012 4938 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5950 BstBI TT'CG_AA Cuts at: 4754 4754 Size: 5950 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5950 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5950 BstYI r'GATC_y Cuts at: 1972 2590 3280 3647 4032 4049 5501 5587 5599 Size: 618 690 367 385 17 1452 86 12 Cuts at: 5599 5945 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 690 618 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2019 2019 Size: 5950 BtgI C'CryG_G Cuts at: 513 1065 2050 513 Size: 552 985 4413 Fragments arranged by size: 4413 985 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5211 Fragments arranged by size: 5211 683 56 BtsI GCAGTG_nn' Cuts at: 3466 4260 4280 3466 Size: 794 20 5136 Fragments arranged by size: 5136 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5950 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2032 2067 529 Size: 35 1468 35 4412 Fragments arranged by size: 4412 1468 35 35 DraI TTT'AAA Cuts at: 3254 3254 Size: 5950 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4957 809 Size: 507 3641 1802 Fragments arranged by size: 3641 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2621 3397 4286 Size: 54 1425 50 275 18 791 776 889 Cuts at: 4286 5915 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 791 776 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3397 1537 Size: 275 1585 4090 Fragments arranged by size: 4090 1585 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2974 3086 3880 1353 Size: 1621 112 794 3423 Fragments arranged by size: 3423 1621 794 112 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4537 4911 5057 387 Size: 1064 138 2948 374 146 1280 Fragments arranged by size: 2948 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2621 3200 4013 4610 5397 5665 1239 Size: 393 989 579 813 597 787 268 1524 Fragments arranged by size: 1524 989 813 787 597 579 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2022 2022 Size: 5950 EcoO109I rG'GnC_Cy Cuts at: 2015 2627 2628 2887 2015 Size: 612 1 259 5078 Fragments arranged by size: 5078 612 259 1 EcoRI G'AATT_C Cuts at: 3088 3259 3637 3088 Size: 171 378 5401 Fragments arranged by size: 5401 378 171 FspI TGC'GCA Cuts at: 4456 4456 Size: 5950 HaeII r_GCGC'y Cuts at: 1678 2328 5097 1678 Size: 650 2769 2531 Fragments arranged by size: 2769 2531 650 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3173 3205 4591 4623 4665 Size: 32 135 32 1601 32 1386 32 42 Cuts at: 4665 4697 1373 Size: 32 2626 Fragments arranged by size: 2626 1601 1386 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3294 3540 669 Size: 988 281 1356 246 3079 Fragments arranged by size: 3079 1356 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5950 HpaI GTT'AAC Cuts at: 3540 3540 Size: 5950 KpnI G_GTAC'C Cuts at: 3275 3275 Size: 5950 MfeI C'AATT_G Cuts at: 3549 3549 Size: 5950 MluI A'CGCG_T Cuts at: 5826 5826 Size: 5950 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2385 5064 5248 1305 Size: 1080 2679 184 2007 Fragments arranged by size: 2679 2007 1080 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4128 Fragments arranged by size: 4128 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2255 2365 2549 2612 3146 3908 4267 Size: 648 1089 110 184 63 534 762 359 Cuts at: 4267 4426 518 Size: 159 2042 Fragments arranged by size: 2042 1089 762 648 534 359 184 159 110 63 MspA1I CmG'CkG Cuts at: 1606 2620 3366 4043 5191 5436 1606 Size: 1014 746 677 1148 245 2120 Fragments arranged by size: 2120 1148 1014 746 677 245 NaeI GCC'GGC Cuts at: 3214 3214 Size: 5950 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5398 NdeI CA'TA_TG Cuts at: 387 387 Size: 5950 NgoMIV G'CCGG_C Cuts at: 3212 3212 Size: 5950 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5950 NotI GC'GGCC_GC Cuts at: 3397 3397 Size: 5950 NspI r_CATG'y Cuts at: 1132 1229 3308 4853 1132 Size: 97 2079 1545 2229 Fragments arranged by size: 2229 2079 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5950 PciI A'CATG_T Cuts at: 4849 4849 Size: 5950 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5950 PmeI GTTT'AAAC Cuts at: 3254 3254 Size: 5950 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2081 2113 4019 4051 5532 5564 2081 Size: 32 1906 32 1481 32 2467 Fragments arranged by size: 2467 1906 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5950 PsiI TTA'TAA Cuts at: 3520 3520 Size: 5950 PspOMI G'GGCC_C Cuts at: 2014 2627 2014 Size: 613 5337 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2903 2935 2903 Size: 32 5918 PstI C_TGCA'G Cuts at: 830 3302 830 Size: 2472 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5950 SalI G'TCGA_C Cuts at: 1936 3292 1936 Size: 1356 4594 SbfI CC_TGCA'GG Cuts at: 3302 3302 Size: 5950 ScaI AGT'ACT Cuts at: 4198 5910 4198 Size: 1712 4238 SfcI C'TryA_G Cuts at: 826 1045 2710 3217 3298 4433 5114 5305 826 Size: 219 1665 507 81 1135 681 191 1471 Fragments arranged by size: 1665 1471 1135 681 507 219 191 81 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5950 SmaI CCC'GGG Cuts at: 1872 2644 3277 1872 Size: 772 633 4545 Fragments arranged by size: 4545 772 633 SmlI C'TyrA_G Cuts at: 820 1017 1943 4054 4955 5217 820 Size: 197 926 2111 901 262 1553 Fragments arranged by size: 2111 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3741 493 Size: 3248 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5950 SphI G_CATG'C Cuts at: 1132 3308 1132 Size: 2176 3774 SspI AAT'ATT Cuts at: 5 52 3874 5 Size: 47 3822 2081 Fragments arranged by size: 3822 2081 47 StuI AGG'CCT Cuts at: 1517 2685 2701 1517 Size: 1168 16 4766 Fragments arranged by size: 4766 1168 16 StyI C'CwwG_G Cuts at: 513 1065 3389 513 Size: 552 2324 3074 Fragments arranged by size: 3074 2324 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2278 2921 3227 3977 3994 4147 4332 Size: 1255 698 643 306 750 17 153 185 Cuts at: 4332 4777 325 Size: 445 1498 Fragments arranged by size: 1498 1255 750 698 643 445 306 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2739 4196 5908 Size: 274 80 33 51 1443 762 1457 1712 Cuts at: 5908 96 Size: 138 Fragments arranged by size: 1712 1457 1443 762 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5910 5942 5910 Size: 32 5918 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5950 XbaI T'CTAG_A Cuts at: 3286 3286 Size: 5950 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2963 3194 2963 Size: 231 5719 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5950 XmaI C'CCGG_G Cuts at: 1870 2642 3275 1870 Size: 772 633 4545 Fragments arranged by size: 4545 772 633 XmnI GAAnn'nnTTC Cuts at: 2385 4079 2385 Size: 1694 4256 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3758 276 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI