(Circular) (Six-base) MAPSORT of: pFN21AB4076.seq Check: 7009 from: 1 to: 6424 pFN21AB4076 6424 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4234 278 Size: 53 83 186 936 2698 2468 Fragments arranged by size: 2698 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3767 1937 Size: 1830 4594 Acc65I G'GTAC_C Cuts at: 3745 3745 Size: 6424 AclI AA'CG_TT Cuts at: 1563 4551 4924 1563 Size: 2988 373 3063 Fragments arranged by size: 3063 2988 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2809 2977 3565 4487 5871 6139 1632 Size: 1177 168 588 922 1384 268 1917 Fragments arranged by size: 1917 1384 1177 922 588 268 168 AfeI AGC'GCT Cuts at: 3663 3663 Size: 6424 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6227 AflIII A'CryG_T Cuts at: 1203 3053 5323 6300 1203 Size: 1850 2270 977 1327 Fragments arranged by size: 2270 1850 1327 977 AgeI A'CCGG_T Cuts at: 5255 5255 Size: 6424 AhdI GACnn_n'nnGTC Cuts at: 5153 5153 Size: 6424 AleI CACnn'nnGTG Cuts at: 1166 2703 1166 Size: 1537 4887 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6424 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2141 2173 2141 Size: 32 6392 AlwNI CAG_nnn'CTG Cuts at: 2270 4167 5739 2270 Size: 1897 1572 2955 Fragments arranged by size: 2955 1897 1572 ApaLI G'TGCA_C Cuts at: 4481 5637 4481 Size: 1156 5268 ApoI r'AATT_y Cuts at: 1493 2238 2819 3279 3531 3733 3959 4111 6220 Size: 745 581 460 252 202 226 152 2109 Cuts at: 6220 1493 Size: 1697 Fragments arranged by size: 2109 1697 745 581 460 252 226 202 152 AseI AT'TA_AT Cuts at: 160 1032 4978 160 Size: 872 3946 1606 Fragments arranged by size: 3946 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6424 AvaI C'yCGr_G Cuts at: 1870 1943 3749 1870 Size: 73 1806 4545 Fragments arranged by size: 4545 1806 73 BamHI G'GATC_C Cuts at: 3754 4121 3754 Size: 367 6057 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3745 5200 618 Size: 325 217 627 1958 1455 1842 Fragments arranged by size: 1958 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2220 3743 1391 Size: 829 1523 4072 Fragments arranged by size: 4072 1523 829 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2516 928 Size: 971 617 4836 Fragments arranged by size: 4836 971 617 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4615 4649 1903 Size: 34 2678 34 3678 Fragments arranged by size: 3678 2678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3441 3683 4316 5526 3441 Size: 242 633 1210 4339 Fragments arranged by size: 4339 1210 633 242 BclI T'GATC_A Cuts at: 1548 1548 Size: 6424 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5035 136 Size: 107 122 71 631 605 3363 1525 Fragments arranged by size: 3363 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3254 6419 3254 Size: 3165 3259 BlpI GC'TnA_GC Cuts at: 2106 2535 3841 2106 Size: 429 1306 4689 Fragments arranged by size: 4689 1306 429 Bme1580I G_kGCm'C Cuts at: 1790 4485 5641 1790 Size: 2695 1156 2573 Fragments arranged by size: 2695 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6424 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4155 5113 442 Size: 416 53 83 399 24 2738 958 1753 Fragments arranged by size: 2738 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6424 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2509 2509 Size: 6424 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2889 3141 3393 3477 3645 5084 1239 Size: 917 733 252 252 84 168 1439 2579 Fragments arranged by size: 2579 1439 917 733 252 252 168 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4549 5414 5712 4549 Size: 865 298 5261 Fragments arranged by size: 5261 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2243 2397 3542 5087 882 Size: 617 744 154 1145 1545 2219 Fragments arranged by size: 2219 1545 1145 744 617 154 BsaAI yAC'GTr Cuts at: 493 1204 4215 493 Size: 711 3011 2702 Fragments arranged by size: 3011 2702 711 BsaBI GATnn'nnATC Cuts at: 6418 6418 Size: 6424 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4231 4613 5248 275 Size: 53 83 186 936 2698 382 635 1451 Fragments arranged by size: 2698 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2196 3501 4857 5255 5529 5676 6087 2196 Size: 1305 1356 398 274 147 411 2533 Fragments arranged by size: 2533 1356 1305 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6199 Fragments arranged by size: 6199 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 1186 Size: 910 5514 BseYI C'CCAG_C Cuts at: 5627 6123 5627 Size: 496 5928 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5851 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3874 4635 5663 1540 Size: 275 186 1873 761 1028 2301 Fragments arranged by size: 2301 1873 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3173 4485 4570 5641 3173 Size: 1312 85 1071 3956 Fragments arranged by size: 3956 1312 1071 85 BsmI GAATG_Cn' Cuts at: 2456 3227 3479 3935 4028 2456 Size: 771 252 456 93 4852 Fragments arranged by size: 4852 771 456 252 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2220 3173 3743 4485 4570 5641 1391 Size: 399 430 953 570 742 85 1071 2174 Fragments arranged by size: 2174 1071 953 742 570 430 399 85 BspEI T'CCGG_A Cuts at: 3501 6087 3501 Size: 2586 3838 BspHI T'CATG_A Cuts at: 3394 4313 6043 3394 Size: 919 1730 3775 Fragments arranged by size: 3775 1730 919 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3779 844 Size: 2935 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4311 1394 Size: 429 2488 3507 Fragments arranged by size: 3507 2488 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3232 4919 5093 65 Size: 1183 1984 1687 174 1396 Fragments arranged by size: 1984 1687 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5068 5255 1700 Size: 110 3258 187 2869 Fragments arranged by size: 3258 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2118 3048 96 Size: 2022 930 3472 Fragments arranged by size: 3472 2022 930 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6424 BssSI C'ACGA_G Cuts at: 4484 5496 4484 Size: 1012 5412 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6424 BstBI TT'CG_AA Cuts at: 5228 5228 Size: 6424 BstEII G'GTnAC_C Cuts at: 1184 2044 1184 Size: 860 5564 BstYI r'GATC_y Cuts at: 1972 3254 3754 4121 4506 4523 5975 6061 6073 Size: 1282 500 367 385 17 1452 86 12 Cuts at: 6073 6419 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1282 500 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4933 Fragments arranged by size: 4933 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5685 Fragments arranged by size: 5685 683 56 BtsI GCAGTG_nn' Cuts at: 2100 3940 4734 4754 2100 Size: 1840 794 20 3770 Fragments arranged by size: 3770 1840 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6424 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3214 3249 3634 3669 529 Size: 35 2650 35 385 35 3284 Fragments arranged by size: 3284 2650 385 35 35 35 DraI TTT'AAA Cuts at: 2314 2589 3728 2314 Size: 275 1139 5010 Fragments arranged by size: 5010 1139 275 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5431 809 Size: 507 4115 1802 Fragments arranged by size: 4115 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3871 4760 6389 Size: 54 1425 50 275 18 2041 889 1629 Cuts at: 6389 8 Size: 43 Fragments arranged by size: 2041 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3871 1537 Size: 275 2059 4090 Fragments arranged by size: 4090 2059 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2208 3540 4354 1353 Size: 855 1332 814 3423 Fragments arranged by size: 3423 1332 855 814 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5011 5385 5531 387 Size: 1064 138 3422 374 146 1280 Fragments arranged by size: 3422 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2809 2889 2977 3141 3393 3477 Size: 393 524 653 80 88 164 252 84 Cuts at: 3477 3565 3645 4487 5084 5871 6139 1239 Size: 88 80 842 597 787 268 1524 Fragments arranged by size: 1524 842 787 653 597 524 393 268 252 164 88 88 84 80 80 EcoO109I rG'GnC_Cy Cuts at: 3213 3213 Size: 6424 EcoRI G'AATT_C Cuts at: 3279 3531 3733 4111 3279 Size: 252 202 378 5592 Fragments arranged by size: 5592 378 252 202 FspI TGC'GCA Cuts at: 4930 4930 Size: 6424 HaeII r_GCGC'y Cuts at: 1678 2836 3665 5571 1678 Size: 1158 829 1906 2531 Fragments arranged by size: 2531 1906 1158 829 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 2309 2341 3160 Size: 32 135 32 428 32 277 32 819 Cuts at: 3160 3192 5065 5097 5139 5171 1373 Size: 32 1873 32 42 32 2626 Fragments arranged by size: 2626 1873 819 428 277 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3768 4014 669 Size: 988 281 1830 246 3079 Fragments arranged by size: 3079 1830 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6424 HpaI GTT'AAC Cuts at: 4014 4014 Size: 6424 KpnI G_GTAC'C Cuts at: 3749 3749 Size: 6424 MfeI C'AATT_G Cuts at: 4023 4023 Size: 6424 MluI A'CGCG_T Cuts at: 6300 6300 Size: 6424 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2181 3520 5538 5722 1305 Size: 876 1339 2018 184 2007 Fragments arranged by size: 2018 2007 1339 876 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4602 Fragments arranged by size: 4602 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2339 2565 2703 2952 3388 3399 3472 Size: 648 1173 226 138 249 436 11 73 Cuts at: 3472 4382 4741 4900 518 Size: 910 359 159 2042 Fragments arranged by size: 2042 1173 910 648 436 359 249 226 159 138 73 11 MspA1I CmG'CkG Cuts at: 1606 2829 2997 3840 4517 5665 5910 1606 Size: 1223 168 843 677 1148 245 2120 Fragments arranged by size: 2120 1223 1148 843 677 245 168 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4933 Fragments arranged by size: 4933 939 552 NdeI CA'TA_TG Cuts at: 387 3385 3469 387 Size: 2998 84 3342 Fragments arranged by size: 3342 2998 84 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6424 NotI GC'GGCC_GC Cuts at: 3871 3871 Size: 6424 NspI r_CATG'y Cuts at: 1132 1229 3057 3645 3782 5327 1132 Size: 97 1828 588 137 1545 2229 Fragments arranged by size: 2229 1828 1545 588 137 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6424 PciI A'CATG_T Cuts at: 3053 5323 3053 Size: 2270 4154 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6424 PfoI T'CCnGG_A Cuts at: 2033 2033 Size: 6424 PmeI GTTT'AAAC Cuts at: 3728 3728 Size: 6424 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4493 4525 6006 6038 4493 Size: 32 1481 32 4879 Fragments arranged by size: 4879 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6424 PsiI TTA'TAA Cuts at: 3994 3994 Size: 6424 PstI C_TGCA'G Cuts at: 830 3776 830 Size: 2946 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6424 PvuII CAG'CTG Cuts at: 2829 2997 2829 Size: 168 6256 SalI G'TCGA_C Cuts at: 1936 3766 1936 Size: 1830 4594 SapI GCTCTTCn'nnn_ Cuts at: 2208 2208 Size: 6424 SbfI CC_TGCA'GG Cuts at: 3776 3776 Size: 6424 ScaI AGT'ACT Cuts at: 2475 2544 4672 6384 2475 Size: 69 2128 1712 2515 Fragments arranged by size: 2515 2128 1712 69 SexAI A'CCwGG_T Cuts at: 2151 2151 Size: 6424 SfcI C'TryA_G Cuts at: 826 1045 3772 4907 5588 5779 826 Size: 219 2727 1135 681 191 1471 Fragments arranged by size: 2727 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6424 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6424 SmaI CCC'GGG Cuts at: 1872 3751 1872 Size: 1879 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4528 5429 5691 820 Size: 197 926 2585 901 262 1553 Fragments arranged by size: 2585 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4215 493 Size: 3722 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6424 SphI G_CATG'C Cuts at: 1132 3782 1132 Size: 2650 3774 SspI AAT'ATT Cuts at: 5 52 4348 5 Size: 47 4296 2081 Fragments arranged by size: 4296 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6424 StyI C'CwwG_G Cuts at: 513 1065 2004 2160 3863 513 Size: 552 939 156 1703 3074 Fragments arranged by size: 3074 1703 939 552 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2477 4451 4468 4621 4806 5251 325 Size: 1255 897 1974 17 153 185 445 1498 Fragments arranged by size: 1974 1498 1255 897 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2118 2473 2542 Size: 274 80 33 51 1443 141 355 69 Cuts at: 2542 3048 4670 6382 96 Size: 506 1622 1712 138 Fragments arranged by size: 1712 1622 1443 506 355 274 141 138 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6384 6416 6384 Size: 32 6392 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6424 XbaI T'CTAG_A Cuts at: 3760 3760 Size: 6424 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6424 XmaI C'CCGG_G Cuts at: 1870 3749 1870 Size: 1879 4545 XmnI GAAnn'nnTTC Cuts at: 3158 3494 4553 3158 Size: 336 1059 5029 Fragments arranged by size: 5029 1059 336 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4232 276 Size: 53 83 186 936 2698 2468 Fragments arranged by size: 2698 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI