(Circular) (Six-base) MAPSORT of: pFN21AB4058.seq Check: 3974 from: 1 to: 6316 pFN21AB4058 6316 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4126 278 Size: 53 83 186 936 2590 2468 Fragments arranged by size: 2590 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3659 1937 Size: 1722 4594 Acc65I G'GTAC_C Cuts at: 3637 3637 Size: 6316 AclI AA'CG_TT Cuts at: 1563 4443 4816 1563 Size: 2880 373 3063 Fragments arranged by size: 3063 2880 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2553 4379 5763 6031 1632 Size: 921 1826 1384 268 1917 Fragments arranged by size: 1917 1826 1384 921 268 AflII C'TTAA_G Cuts at: 820 1017 3518 820 Size: 197 2501 3618 Fragments arranged by size: 3618 2501 197 AflIII A'CryG_T Cuts at: 1203 5215 6192 1203 Size: 4012 977 1327 Fragments arranged by size: 4012 1327 977 AgeI A'CCGG_T Cuts at: 5147 5147 Size: 6316 AhdI GACnn_n'nnGTC Cuts at: 5045 5045 Size: 6316 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6316 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6316 AlwNI CAG_nnn'CTG Cuts at: 4059 5631 4059 Size: 1572 4744 ApaI G_GGCC'C Cuts at: 2359 2359 Size: 6316 ApaLI G'TGCA_C Cuts at: 3212 4373 5529 3212 Size: 1161 1156 3999 Fragments arranged by size: 3999 1161 1156 ApoI r'AATT_y Cuts at: 1493 3625 3851 4003 6112 1493 Size: 2132 226 152 2109 1697 Fragments arranged by size: 2132 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4870 160 Size: 872 3838 1606 Fragments arranged by size: 3838 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6316 AvaI C'yCGr_G Cuts at: 1870 1943 2891 3641 1870 Size: 73 948 750 4545 Fragments arranged by size: 4545 948 750 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3434 3467 3434 Size: 33 6283 BamHI G'GATC_C Cuts at: 2134 2913 3072 3646 4013 2134 Size: 779 159 574 367 4437 Fragments arranged by size: 4437 779 574 367 159 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3637 5092 618 Size: 325 217 627 1850 1455 1842 Fragments arranged by size: 1850 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2359 2396 2890 3635 1391 Size: 968 37 494 745 4072 Fragments arranged by size: 4072 968 745 494 37 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3197 3485 928 Size: 971 1298 288 3759 Fragments arranged by size: 3759 1298 971 288 BbvCI CC'TCA_GC Cuts at: 2037 2037 Size: 6316 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4507 4541 1903 Size: 34 2570 34 3678 Fragments arranged by size: 3678 2570 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4208 5418 4208 Size: 1210 5106 BclI T'GATC_A Cuts at: 1548 3103 1548 Size: 1555 4761 BfrBI ATG'CAT Cuts at: 2089 3422 2089 Size: 1333 4983 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4927 136 Size: 107 122 71 631 605 3255 1525 Fragments arranged by size: 3255 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6311 6311 Size: 6316 BlpI GC'TnA_GC Cuts at: 3733 3733 Size: 6316 Bme1580I G_kGCm'C Cuts at: 1790 2359 3216 4377 5533 1790 Size: 569 857 1161 1156 2573 Fragments arranged by size: 2573 1161 1156 857 569 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6316 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2399 4047 5005 Size: 416 53 83 399 24 982 1648 958 Cuts at: 5005 442 Size: 1753 Fragments arranged by size: 1753 1648 982 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6316 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2430 2571 2939 3253 3449 4976 1239 Size: 1191 141 368 314 196 1527 2579 Fragments arranged by size: 2579 1527 1191 368 314 196 141 Bpu10I CC'TnA_GC Cuts at: 2037 2641 3456 2037 Size: 604 815 4897 Fragments arranged by size: 4897 815 604 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3614 4441 5306 5604 3614 Size: 827 865 298 4326 Fragments arranged by size: 4326 865 827 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3425 4979 882 Size: 617 1926 1554 2219 Fragments arranged by size: 2219 1926 1554 617 BsaAI yAC'GTr Cuts at: 493 1204 3211 4107 493 Size: 711 2007 896 2702 Fragments arranged by size: 2702 2007 896 711 BsaBI GATnn'nnATC Cuts at: 6310 6310 Size: 6316 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4123 4505 5140 275 Size: 53 83 186 936 2590 382 635 1451 Fragments arranged by size: 2590 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3504 4749 5147 5421 5568 5979 3504 Size: 1245 398 274 147 411 3841 Fragments arranged by size: 3841 1245 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2246 2276 3071 3101 3401 Size: 30 165 30 843 30 795 30 300 Cuts at: 3401 3431 1178 Size: 30 4063 Fragments arranged by size: 4063 843 795 300 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2251 2292 2849 2909 3131 1186 Size: 1065 41 557 60 222 4371 Fragments arranged by size: 4371 1065 557 222 60 41 BseYI C'CCAG_C Cuts at: 2879 5519 6015 2879 Size: 2640 496 3180 Fragments arranged by size: 3180 2640 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2555 2728 1163 Size: 573 819 173 4751 Fragments arranged by size: 4751 819 573 173 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3766 4527 5555 1540 Size: 275 186 1765 761 1028 2301 Fragments arranged by size: 2301 1765 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3216 4377 4462 5533 3216 Size: 1161 85 1071 3999 Fragments arranged by size: 3999 1161 1071 85 BsmI GAATG_Cn' Cuts at: 3827 3920 3827 Size: 93 6223 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2359 2396 2890 3216 3635 4377 4462 Size: 399 569 37 494 326 419 742 85 Cuts at: 4462 5533 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 569 494 419 399 326 85 37 BspEI T'CCGG_A Cuts at: 5979 5979 Size: 6316 BspHI T'CATG_A Cuts at: 4205 5935 4205 Size: 1730 4586 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3671 844 Size: 2827 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2018 4203 1394 Size: 429 195 2185 3507 Fragments arranged by size: 3507 2185 429 195 BsrDI GCAATG_nn' Cuts at: 65 1248 3144 4811 4985 65 Size: 1183 1896 1667 174 1396 Fragments arranged by size: 1896 1667 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2065 4960 5147 1700 Size: 110 255 2895 187 2869 Fragments arranged by size: 2895 2869 255 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6316 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6316 BssSI C'ACGA_G Cuts at: 4376 5388 4376 Size: 1012 5304 BstAPI GCAn_nnn'nTGC Cuts at: 794 2930 794 Size: 2136 4180 BstBI TT'CG_AA Cuts at: 5120 5120 Size: 6316 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6316 BstYI r'GATC_y Cuts at: 1972 2134 2913 3072 3646 4013 4398 4415 5867 Size: 162 779 159 574 367 385 17 1452 Cuts at: 5867 5953 5965 6311 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 779 574 385 367 346 162 159 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3338 3338 Size: 6316 BtgI C'CryG_G Cuts at: 513 1065 2421 513 Size: 552 1356 4408 Fragments arranged by size: 4408 1356 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5577 Fragments arranged by size: 5577 683 56 BtsI GCAGTG_nn' Cuts at: 2063 3832 4626 4646 2063 Size: 1769 794 20 3733 Fragments arranged by size: 3733 1769 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6316 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6281 DraI TTT'AAA Cuts at: 3620 3620 Size: 6316 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5323 809 Size: 507 4007 1802 Fragments arranged by size: 4007 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2802 3612 3763 Size: 54 1425 50 275 18 972 810 151 Cuts at: 3763 4652 6281 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 972 889 810 275 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3763 1537 Size: 275 1951 4090 Fragments arranged by size: 4090 1951 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2810 2939 3062 4246 1353 Size: 1457 129 123 1184 3423 Fragments arranged by size: 3423 1457 1184 129 123 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2184 4903 5277 5423 387 Size: 1064 138 595 2719 374 146 1280 Fragments arranged by size: 2719 1280 1064 595 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2430 2553 2571 2939 3253 3449 4379 Size: 393 798 123 18 368 314 196 930 Cuts at: 4379 4976 5763 6031 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 930 798 787 597 393 368 314 268 196 123 18 EcoNI CCTnn'n_nnAGG Cuts at: 2279 2279 Size: 6316 EcoO109I rG'GnC_Cy Cuts at: 2355 2356 2427 2566 2966 3373 3400 2355 Size: 1 71 139 400 407 27 5271 Fragments arranged by size: 5271 407 400 139 71 27 1 EcoRI G'AATT_C Cuts at: 3625 4003 3625 Size: 378 5938 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2704 2704 Size: 6316 FspI TGC'GCA Cuts at: 4822 4822 Size: 6316 HaeII r_GCGC'y Cuts at: 1678 2761 5463 1678 Size: 1083 2702 2531 Fragments arranged by size: 2702 2531 1083 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2246 2278 2624 2656 3171 Size: 32 135 32 674 32 346 32 515 Cuts at: 3171 3203 3275 3307 4957 4989 5031 5063 1373 Size: 32 72 32 1650 32 42 32 2626 Fragments arranged by size: 2626 1650 674 515 346 135 72 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3660 3906 669 Size: 988 281 1722 246 3079 Fragments arranged by size: 3079 1722 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6316 HpaI GTT'AAC Cuts at: 3906 3906 Size: 6316 KpnI G_GTAC'C Cuts at: 3641 3641 Size: 6316 MfeI C'AATT_G Cuts at: 3915 3915 Size: 6316 MluI A'CGCG_T Cuts at: 6192 6192 Size: 6316 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3405 5430 5614 1305 Size: 2100 2025 184 2007 Fragments arranged by size: 2100 2025 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2804 10 Size: 54 1425 343 972 3522 Fragments arranged by size: 3522 1425 972 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2810 3311 3587 4274 4633 4792 518 Size: 648 1644 501 276 687 359 159 2042 Fragments arranged by size: 2042 1644 687 648 501 359 276 159 MspA1I CmG'CkG Cuts at: 1606 2041 2144 2232 2667 2764 2772 2845 3032 Size: 435 103 88 435 97 8 73 187 Cuts at: 3032 3732 4409 5557 5802 1606 Size: 700 677 1148 245 2120 Fragments arranged by size: 2120 1148 700 677 435 435 245 187 103 97 88 73 8 NcoI C'CATG_G Cuts at: 513 1065 2421 513 Size: 552 1356 4408 Fragments arranged by size: 4408 1356 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6316 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6316 NotI GC'GGCC_GC Cuts at: 3763 3763 Size: 6316 NsiI A_TGCA'T Cuts at: 2091 3424 2091 Size: 1333 4983 NspI r_CATG'y Cuts at: 1132 1229 2048 3674 5219 1132 Size: 97 819 1626 1545 2229 Fragments arranged by size: 2229 1626 1545 819 97 PasI CC'CwG_GG Cuts at: 1348 2281 2510 2597 1348 Size: 933 229 87 5067 Fragments arranged by size: 5067 933 229 87 PciI A'CATG_T Cuts at: 5215 5215 Size: 6316 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2342 3610 1108 Size: 902 332 1268 3814 Fragments arranged by size: 3814 1268 902 332 PfoI T'CCnGG_A Cuts at: 2916 2916 Size: 6316 PmeI GTTT'AAAC Cuts at: 3620 3620 Size: 6316 PmlI CAC'GTG Cuts at: 3211 3211 Size: 6316 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4385 4417 5898 5930 4385 Size: 32 1481 32 4771 Fragments arranged by size: 4771 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2427 2966 3400 2427 Size: 539 434 5343 Fragments arranged by size: 5343 539 434 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6316 PsiI TTA'TAA Cuts at: 3886 3886 Size: 6316 PspOMI G'GGCC_C Cuts at: 2355 2355 Size: 6316 PstI C_TGCA'G Cuts at: 830 3668 830 Size: 2838 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6316 PvuII CAG'CTG Cuts at: 2667 3032 2667 Size: 365 5951 SalI G'TCGA_C Cuts at: 1936 3658 1936 Size: 1722 4594 SanDI GG'GwC_CC Cuts at: 2427 2427 Size: 6316 SbfI CC_TGCA'GG Cuts at: 3668 3668 Size: 6316 ScaI AGT'ACT Cuts at: 2780 4564 6276 2780 Size: 1784 1712 2820 Fragments arranged by size: 2820 1784 1712 SfcI C'TryA_G Cuts at: 826 1045 3664 4799 5480 5671 826 Size: 219 2619 1135 681 191 1471 Fragments arranged by size: 2619 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6316 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6316 SmaI CCC'GGG Cuts at: 1872 3643 1872 Size: 1771 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3518 3593 4420 5321 5583 820 Size: 197 926 1575 75 827 901 262 1553 Fragments arranged by size: 1575 1553 926 901 827 262 197 75 SnaBI TAC'GTA Cuts at: 493 4107 493 Size: 3614 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6316 SphI G_CATG'C Cuts at: 1132 2048 3674 1132 Size: 916 1626 3774 Fragments arranged by size: 3774 1626 916 SspI AAT'ATT Cuts at: 5 52 4240 5 Size: 47 4188 2081 Fragments arranged by size: 4188 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6316 StyI C'CwwG_G Cuts at: 513 1065 2341 2421 3369 3755 513 Size: 552 1276 80 948 386 3074 Fragments arranged by size: 3074 1276 948 552 386 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3249 4343 4360 4513 4698 5143 325 Size: 1255 1669 1094 17 153 185 445 1498 Fragments arranged by size: 1669 1498 1255 1094 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2778 4562 6274 Size: 274 80 33 51 1443 801 1784 1712 Cuts at: 6274 96 Size: 138 Fragments arranged by size: 1784 1712 1443 801 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3071 3103 6276 6308 3071 Size: 32 3173 32 3079 Fragments arranged by size: 3173 3079 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6316 XbaI T'CTAG_A Cuts at: 3526 3652 3526 Size: 126 6190 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6316 XmaI C'CCGG_G Cuts at: 1870 3641 1870 Size: 1771 4545 XmnI GAAnn'nnTTC Cuts at: 4445 4445 Size: 6316 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4124 276 Size: 53 83 186 936 2590 2468 Fragments arranged by size: 2590 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI