Restriction map for
pFN21AB4018
(Circular) (Six-base) MAPSORT of: pFN21AB4018.seq Check: 1356 from: 1 to: 7387
pFN21AB4018 7387 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2094 5197 278
Size: 53 83 186 936 558 3103 2468
Fragments arranged by size:
3103 2468 936 558 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2094 4730 1937
Size: 157 2636 4594
Fragments arranged by size:
4594 2636 157
Acc65I G'GTAC_C
Cuts at: 4708 4708
Size: 7387
AclI AA'CG_TT
Cuts at: 1563 5514 5887 1563
Size: 3951 373 3063
Fragments arranged by size:
3951 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2618 4064 5450 6834 7102 1632
Size: 986 1446 1386 1384 268 1917
Fragments arranged by size:
1917 1446 1386 1384 986 268
AflII C'TTAA_G
Cuts at: 820 1017 3028 820
Size: 197 2011 5179
Fragments arranged by size:
5179 2011 197
AflIII A'CryG_T
Cuts at: 1203 3226 6286 7263 1203
Size: 2023 3060 977 1327
Fragments arranged by size:
3060 2023 1327 977
AgeI A'CCGG_T
Cuts at: 6218 6218
Size: 7387
AhdI GACnn_n'nnGTC
Cuts at: 6116 6116
Size: 7387
AleI CACnn'nnGTG
Cuts at: 1166 3156 1166
Size: 1990 5397
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3654 1184
Size: 2470 4917
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3786 3818 3786
Size: 32 7355
AlwNI CAG_nnn'CTG
Cuts at: 5130 6702 5130
Size: 1572 5815
ApaLI G'TGCA_C
Cuts at: 5444 6600 5444
Size: 1156 6231
ApoI r'AATT_y
Cuts at: 1493 2946 3014 3294 3678 3980 4124 4282 4557
Size: 1453 68 280 384 302 144 158 275
Cuts at: 4557 4575 4696 4922 5074 7183 1493
Size: 18 121 226 152 2109 1697
Fragments arranged by size:
2109 1697 1453 384 302 280 275 226
158 152 144 121 68 18
AseI AT'TA_AT
Cuts at: 160 1032 5941 160
Size: 872 4909 1606
Fragments arranged by size:
4909 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7387
AvaI C'yCGr_G
Cuts at: 1870 1943 4712 1870
Size: 73 2769 4545
Fragments arranged by size:
4545 2769 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4190 4223 4190
Size: 33 7354
BamHI G'GATC_C
Cuts at: 4717 5084 4717
Size: 367 7020
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 3737 4017 4708 6163
Size: 325 217 627 291 1659 280 691 1455
Cuts at: 6163 618
Size: 1842
Fragments arranged by size:
1842 1659 1455 691 627 325 291 280
217
BanII G_rGCy'C
Cuts at: 1391 3407 3494 4706 1391
Size: 2016 87 1212 4072
Fragments arranged by size:
4072 2016 1212 87
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2169 3179 3602 928
Size: 971 270 1010 423 4713
Fragments arranged by size:
4713 1010 971 423 270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5578 5612 1903
Size: 34 3641 34 3678
Fragments arranged by size:
3678 3641 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5279 6489 5279
Size: 1210 6177
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7387
BfrBI ATG'CAT
Cuts at: 3349 3349
Size: 7387
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5998 136
Size: 107 122 71 631 605 4326 1525
Fragments arranged by size:
4326 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3113 4535 7382 3113
Size: 1422 2847 3118
Fragments arranged by size:
3118 2847 1422
BlpI GC'TnA_GC
Cuts at: 4804 4804
Size: 7387
Bme1580I G_kGCm'C
Cuts at: 1790 5448 6604 1790
Size: 3658 1156 2573
Fragments arranged by size:
3658 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3229 1318
Size: 1911 5476
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5118 6076 442
Size: 416 53 83 399 24 3701 958 1753
Fragments arranged by size:
3701 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4499 1056
Size: 3443 3944
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2526 4370 6047 1239
Size: 1287 1844 1677 2579
Fragments arranged by size:
2579 1844 1677 1287
Bpu10I CC'TnA_GC
Cuts at: 2919 2919
Size: 7387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3176 5512 6377 6675 3176
Size: 2336 865 298 3888
Fragments arranged by size:
3888 2336 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6050 882
Size: 617 4551 2219
Fragments arranged by size:
4551 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2314 5178 493
Size: 711 1110 2864 2702
Fragments arranged by size:
2864 2702 1110 711
BsaBI GATnn'nnATC
Cuts at: 2307 2913 3366 4417 7381 2307
Size: 606 453 1051 2964 2313
Fragments arranged by size:
2964 2313 1051 606 453
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2091 5194 5576 6211
Size: 53 83 186 936 558 3103 382 635
Cuts at: 6211 275
Size: 1451
Fragments arranged by size:
3103 1451 936 635 558 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5820 6218 6492 6639 7050 5820
Size: 398 274 147 411 6157
Fragments arranged by size:
6157 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3939 3969 1178
Size: 30 165 30 2536 30 4596
Fragments arranged by size:
4596 2536 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7387
BseYI C'CCAG_C
Cuts at: 3406 6590 7086 3406
Size: 3184 496 3707
Fragments arranged by size:
3707 3184 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 2463 1163
Size: 573 462 265 6087
Fragments arranged by size:
6087 573 462 265
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4837 5598 6626 1540
Size: 275 186 2836 761 1028 2301
Fragments arranged by size:
2836 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3494 4078 5448 5533 6604 3494
Size: 584 1370 85 1071 4277
Fragments arranged by size:
4277 1370 1071 584 85
BsmI GAATG_Cn'
Cuts at: 3349 4521 4898 4991 3349
Size: 1172 377 93 5745
Fragments arranged by size:
5745 1172 377 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4262 4262
Size: 7387
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3407 3494 4078 4706 5448 5533 6604
Size: 399 1617 87 584 628 742 85 1071
Cuts at: 6604 1391
Size: 2174
Fragments arranged by size:
2174 1617 1071 742 628 584 399 87
85
BspEI T'CCGG_A
Cuts at: 7050 7050
Size: 7387
BspHI T'CATG_A
Cuts at: 2193 3052 3778 4672 5276 7006 2193
Size: 859 726 894 604 1730 2574
Fragments arranged by size:
2574 1730 894 859 726 604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4742 844
Size: 3898 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5274 1394
Size: 429 3451 3507
Fragments arranged by size:
3507 3451 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5882 6056 65
Size: 1183 4634 174 1396
Fragments arranged by size:
4634 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6031 6218 1700
Size: 110 4221 187 2869
Fragments arranged by size:
4221 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3815 4656 96
Size: 3719 841 2827
Fragments arranged by size:
3719 2827 841
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7387
BssSI C'ACGA_G
Cuts at: 5447 6459 5447
Size: 1012 6375
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7387
BstBI TT'CG_AA
Cuts at: 6191 6191
Size: 7387
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7387
BstXI CCAn_nnnn'nTGG
Cuts at: 3506 3506
Size: 7387
BstYI r'GATC_y
Cuts at: 1972 3113 3124 4535 4592 4717 5084 5469 5486
Size: 1141 11 1411 57 125 367 385 17
Cuts at: 5486 6938 7024 7036 7382 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1411 1141 385 367 346 125
86 57 17 12 11
Bsu36I CC'TnA_GG
Cuts at: 3475 3475
Size: 7387
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6835
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6648
Fragments arranged by size:
6648 683 56
BtsI GCAGTG_nn'
Cuts at: 2363 2607 2610 3105 4903 5697 5717 2363
Size: 244 3 495 1798 794 20 4033
Fragments arranged by size:
4033 1798 794 495 244 20 3
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7387
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3288 3323 529
Size: 35 2724 35 4593
Fragments arranged by size:
4593 2724 35 35
DraI TTT'AAA
Cuts at: 4691 4691
Size: 7387
DraIII CAC_nnn'GTG
Cuts at: 2752 2752
Size: 7387
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6394 809
Size: 507 5078 1802
Fragments arranged by size:
5078 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3004 4834 5723
Size: 54 1425 50 275 18 1174 1830 889
Cuts at: 5723 7352 8
Size: 1629 43
Fragments arranged by size:
1830 1629 1425 1174 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4834 1537
Size: 275 3022 4090
Fragments arranged by size:
4090 3022 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4297 5317 1353
Size: 2944 1020 3423
Fragments arranged by size:
3423 2944 1020
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5974 6348 6494 387
Size: 1064 138 4385 374 146 1280
Fragments arranged by size:
4385 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3492 3492
Size: 7387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2526 2618 4064 4370 5450 6047 6834
Size: 393 894 92 1446 306 1080 597 787
Cuts at: 6834 7102 1239
Size: 268 1524
Fragments arranged by size:
1524 1446 1080 894 787 597 393 306
268 92
EcoRI G'AATT_C
Cuts at: 2946 4696 5074 2946
Size: 1750 378 5259
Fragments arranged by size:
5259 1750 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3346 4062 3346
Size: 716 6671
FspI TGC'GCA
Cuts at: 5893 5893
Size: 7387
HaeII r_GCGC'y
Cuts at: 1678 6534 1678
Size: 4856 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3213 3245 3251 3283 4261
Size: 32 135 32 1641 32 6 32 978
Cuts at: 4261 4293 4347 4379 6028 6060 6102 6134 1373
Size: 32 54 32 1649 32 42 32 2626
Fragments arranged by size:
2626 1649 1641 978 135 54 42 32
32 32 32 32 32 32 32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2113 3533 4731 4977 669
Size: 988 281 175 1420 1198 246 3079
Fragments arranged by size:
3079 1420 1198 988 281 246 175
HindIII A'AGCT_T
Cuts at: 748 2258 3864 748
Size: 1510 1606 4271
Fragments arranged by size:
4271 1606 1510
HpaI GTT'AAC
Cuts at: 2113 4977 2113
Size: 2864 4523
KpnI G_GTAC'C
Cuts at: 4712 4712
Size: 7387
MfeI C'AATT_G
Cuts at: 2496 3838 4986 2496
Size: 1342 1148 4897
Fragments arranged by size:
4897 1342 1148
MluI A'CGCG_T
Cuts at: 7263 7263
Size: 7387
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2841 3641 6501 6685 1305
Size: 1536 800 2860 184 2007
Fragments arranged by size:
2860 2007 1536 800 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3006 10
Size: 54 1425 343 1174 4391
Fragments arranged by size:
4391 1425 1174 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3156 3636 3694 5345 5704 5863 518
Size: 648 1990 480 58 1651 359 159 2042
Fragments arranged by size:
2042 1990 1651 648 480 359 159 58
MspA1I CmG'CkG
Cuts at: 1606 2076 2555 4803 5480 6628 6873 1606
Size: 470 479 2248 677 1148 245 2120
Fragments arranged by size:
2248 2120 1148 677 479 470 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6835
NdeI CA'TA_TG
Cuts at: 387 2347 3633 387
Size: 1960 1286 4141
Fragments arranged by size:
4141 1960 1286
NheI G'CTAG_C
Cuts at: 1052 4495 1052
Size: 3443 3944
NotI GC'GGCC_GC
Cuts at: 4834 4834
Size: 7387
NsiI A_TGCA'T
Cuts at: 3351 3351
Size: 7387
NspI r_CATG'y
Cuts at: 1132 1229 3645 4745 6290 1132
Size: 97 2416 1100 1545 2229
Fragments arranged by size:
2416 2229 1545 1100 97
PacI TTA_AT'TAA
Cuts at: 4137 4137
Size: 7387
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7387
PciI A'CATG_T
Cuts at: 6286 6286
Size: 7387
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7387
PmeI GTTT'AAAC
Cuts at: 4691 4691
Size: 7387
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5456 5488 6969 7001 5456
Size: 32 1481 32 5842
Fragments arranged by size:
5842 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2326 1613
Size: 713 6674
PsiI TTA'TAA
Cuts at: 4957 4957
Size: 7387
PstI C_TGCA'G
Cuts at: 830 2370 4739 830
Size: 1540 2369 3478
Fragments arranged by size:
3478 2369 1540
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7387
PvuII CAG'CTG
Cuts at: 2555 2555
Size: 7387
SacI G_AGCT'C
Cuts at: 3494 3494
Size: 7387
SalI G'TCGA_C
Cuts at: 1936 4729 1936
Size: 2793 4594
SbfI CC_TGCA'GG
Cuts at: 4739 4739
Size: 7387
ScaI AGT'ACT
Cuts at: 3725 5635 7347 3725
Size: 1910 1712 3765
Fragments arranged by size:
3765 1910 1712
SfcI C'TryA_G
Cuts at: 826 1045 2366 4735 5870 6551 6742 826
Size: 219 1321 2369 1135 681 191 1471
Fragments arranged by size:
2369 1471 1321 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7387
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7387
SmaI CCC'GGG
Cuts at: 1872 4714 1872
Size: 2842 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3028 3191 5491 6392 6654 820
Size: 197 926 1085 163 2300 901 262 1553
Fragments arranged by size:
2300 1553 1085 926 901 262 197 163
SnaBI TAC'GTA
Cuts at: 493 5178 493
Size: 4685 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7387
SphI G_CATG'C
Cuts at: 1132 4745 1132
Size: 3613 3774
SspI AAT'ATT
Cuts at: 5 52 2221 5311 5
Size: 47 2169 3090 2081
Fragments arranged by size:
3090 2169 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7387
StyI C'CwwG_G
Cuts at: 513 1065 3154 3974 4405 4826 513
Size: 552 2089 820 431 421 3074
Fragments arranged by size:
3074 2089 820 552 431 421
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2229 2673 3626 3654 5414 5431 5584
Size: 1255 649 444 953 28 1760 17 153
Cuts at: 5584 5769 6214 325
Size: 185 445 1498
Fragments arranged by size:
1760 1498 1255 953 649 445 444 185
153 28 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3723 3815 4656
Size: 274 80 33 51 1443 1746 92 841
Cuts at: 4656 5633 7345 96
Size: 977 1712 138
Fragments arranged by size:
1746 1712 1443 977 841 274 138 92
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3939 3971 7347 7379 3939
Size: 32 3376 32 3947
Fragments arranged by size:
3947 3376 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2839 4601 1316
Size: 1523 1762 4102
Fragments arranged by size:
4102 1762 1523
XbaI T'CTAG_A
Cuts at: 4723 4723
Size: 7387
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7387
XmaI C'CCGG_G
Cuts at: 1870 4712 1870
Size: 2842 4545
XmnI GAAnn'nnTTC
Cuts at: 2513 5516 2513
Size: 3003 4384
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2092 5195 276
Size: 53 83 186 936 558 3103 2468
Fragments arranged by size:
3103 2468 936 558 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PacI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BplI
BsiWI BstZ17I EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI