(Circular) (Six-base) MAPSORT of: pFN21AB4014.seq Check: 5621 from: 1 to: 6112 pFN21AB4014 6112 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2758 2758 Size: 6112 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3922 278 Size: 53 83 186 936 2386 2468 Fragments arranged by size: 2468 2386 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3455 1937 Size: 1518 4594 Acc65I G'GTAC_C Cuts at: 2932 3433 2932 Size: 501 5611 AclI AA'CG_TT Cuts at: 1563 4239 4612 1563 Size: 2676 373 3063 Fragments arranged by size: 3063 2676 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3012 3149 4175 5559 5827 1632 Size: 1380 137 1026 1384 268 1917 Fragments arranged by size: 1917 1384 1380 1026 268 137 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5915 AflIII A'CryG_T Cuts at: 1203 5011 5988 1203 Size: 3808 977 1327 Fragments arranged by size: 3808 1327 977 AgeI A'CCGG_T Cuts at: 4943 4943 Size: 6112 AhdI GACnn_n'nnGTC Cuts at: 4841 4841 Size: 6112 AleI CACnn'nnGTG Cuts at: 1166 3243 1166 Size: 2077 4035 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6112 AlwNI CAG_nnn'CTG Cuts at: 2177 2345 2399 2711 2759 3855 5427 2177 Size: 168 54 312 48 1096 1572 2862 Fragments arranged by size: 2862 1572 1096 312 168 54 48 ApaLI G'TGCA_C Cuts at: 4169 5325 4169 Size: 1156 4956 ApoI r'AATT_y Cuts at: 1493 3254 3421 3647 3799 5908 1493 Size: 1761 167 226 152 2109 1697 Fragments arranged by size: 2109 1761 1697 226 167 152 AseI AT'TA_AT Cuts at: 160 1032 4666 160 Size: 872 3634 1606 Fragments arranged by size: 3634 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 AvaI C'yCGr_G Cuts at: 1870 1943 3437 1870 Size: 73 1494 4545 Fragments arranged by size: 4545 1494 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2157 2190 2157 Size: 33 6079 BamHI G'GATC_C Cuts at: 3442 3809 3442 Size: 367 5745 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2302 2932 2984 3318 3433 Size: 325 217 627 515 630 52 334 115 Cuts at: 3433 4888 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 630 627 515 334 325 217 115 52 BanII G_rGCy'C Cuts at: 1391 2393 2978 3431 1391 Size: 1002 585 453 4072 Fragments arranged by size: 4072 1002 585 453 BbeI G_GCGC'C Cuts at: 3322 3322 Size: 6112 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5141 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4303 4337 1903 Size: 34 2366 34 3678 Fragments arranged by size: 3678 2366 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4004 5214 4004 Size: 1210 4902 BclI T'GATC_A Cuts at: 1548 1548 Size: 6112 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2982 4723 136 Size: 107 122 71 631 605 1310 1741 1525 Fragments arranged by size: 1741 1525 1310 631 605 122 107 71 BglII A'GATC_T Cuts at: 6107 6107 Size: 6112 BlpI GC'TnA_GC Cuts at: 3529 3529 Size: 6112 Bme1580I G_kGCm'C Cuts at: 1790 2900 3266 4173 5329 1790 Size: 1110 366 907 1156 2573 Fragments arranged by size: 2573 1156 1110 907 366 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6112 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2387 3843 4801 Size: 416 53 83 399 24 970 1456 958 Cuts at: 4801 442 Size: 1753 Fragments arranged by size: 1753 1456 970 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6112 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4772 1239 Size: 3533 2579 Bpu10I CC'TnA_GC Cuts at: 2421 2421 Size: 6112 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2268 4237 5102 5400 2268 Size: 1969 865 298 2980 Fragments arranged by size: 2980 1969 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2703 2746 4775 882 Size: 617 1204 43 2029 2219 Fragments arranged by size: 2219 2029 1204 617 43 BsaAI yAC'GTr Cuts at: 493 1204 3903 493 Size: 711 2699 2702 Fragments arranged by size: 2702 2699 711 BsaBI GATnn'nnATC Cuts at: 6106 6106 Size: 6112 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3319 3919 4301 4936 Size: 53 83 186 936 1786 600 382 635 Cuts at: 4936 275 Size: 1451 Fragments arranged by size: 1786 1451 936 635 600 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2965 4545 4943 5217 5364 5775 2965 Size: 1580 398 274 147 411 3302 Fragments arranged by size: 3302 1580 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3385 3415 1178 Size: 30 165 30 1982 30 3875 Fragments arranged by size: 3875 1982 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2841 3036 3304 1186 Size: 1655 195 268 3994 Fragments arranged by size: 3994 1655 268 195 BseYI C'CCAG_C Cuts at: 2233 2457 2843 2855 2937 5315 5811 2233 Size: 224 386 12 82 2378 496 2534 Fragments arranged by size: 2534 2378 496 386 224 82 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2634 2969 1163 Size: 573 898 335 4306 Fragments arranged by size: 4306 898 573 335 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3562 4323 5351 1540 Size: 275 186 1561 761 1028 2301 Fragments arranged by size: 2301 1561 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4173 4258 5329 4173 Size: 85 1071 4956 Fragments arranged by size: 4956 1071 85 BsmI GAATG_Cn' Cuts at: 3623 3716 3623 Size: 93 6019 BsmBI CGTCTCn'nnnn_ Cuts at: 2675 2675 Size: 6112 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2393 2900 2978 3266 3431 4173 4258 Size: 399 603 507 78 288 165 742 85 Cuts at: 4258 5329 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 603 507 399 288 165 85 78 BspEI T'CCGG_A Cuts at: 5775 5775 Size: 6112 BspHI T'CATG_A Cuts at: 3285 4001 5731 3285 Size: 716 1730 3666 Fragments arranged by size: 3666 1730 716 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2486 2685 2758 3467 844 Size: 1642 199 73 709 3489 Fragments arranged by size: 3489 1642 709 199 73 BsrBI CCG'CTC Cuts at: 1394 1823 3999 1394 Size: 429 2176 3507 Fragments arranged by size: 3507 2176 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2358 3000 3378 4607 4781 65 Size: 1183 1110 642 378 1229 174 1396 Fragments arranged by size: 1396 1229 1183 1110 642 378 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2782 3276 4756 4943 1700 Size: 110 972 494 1480 187 2869 Fragments arranged by size: 2869 1480 972 494 187 110 BsrGI T'GTAC_A Cuts at: 96 2320 96 Size: 2224 3888 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6112 BssSI C'ACGA_G Cuts at: 3139 4172 5184 3139 Size: 1033 1012 4067 Fragments arranged by size: 4067 1033 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2648 2759 794 Size: 1854 111 4147 Fragments arranged by size: 4147 1854 111 BstBI TT'CG_AA Cuts at: 4916 4916 Size: 6112 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6112 BstYI r'GATC_y Cuts at: 1972 2597 3442 3809 4194 4211 5663 5749 5761 Size: 625 845 367 385 17 1452 86 12 Cuts at: 5761 6107 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 845 625 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4621 Fragments arranged by size: 4621 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5373 Fragments arranged by size: 5373 683 56 BtsI GCAGTG_nn' Cuts at: 3628 4422 4442 3628 Size: 794 20 5298 Fragments arranged by size: 5298 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6112 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6077 DraI TTT'AAA Cuts at: 3115 3416 3115 Size: 301 5811 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5119 809 Size: 507 3803 1802 Fragments arranged by size: 3803 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3559 4448 6077 Size: 54 1425 50 275 18 1729 889 1629 Cuts at: 6077 8 Size: 43 Fragments arranged by size: 1729 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3559 1537 Size: 275 1747 4090 Fragments arranged by size: 4090 1747 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2187 4042 1353 Size: 834 1855 3423 Fragments arranged by size: 3423 1855 834 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4699 5073 5219 387 Size: 1064 138 3110 374 146 1280 Fragments arranged by size: 3110 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3012 3149 4175 4772 5559 5827 1239 Size: 393 1380 137 1026 597 787 268 1524 Fragments arranged by size: 1524 1380 1026 787 597 393 268 137 EcoRI G'AATT_C Cuts at: 3421 3799 3421 Size: 378 5734 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3127 3127 Size: 6112 FspI TGC'GCA Cuts at: 4618 4618 Size: 6112 HaeII r_GCGC'y Cuts at: 1678 2312 2919 3322 5259 1678 Size: 634 607 403 1937 2531 Fragments arranged by size: 2531 1937 634 607 403 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3058 3090 3203 3235 4753 Size: 32 135 32 1486 32 113 32 1518 Cuts at: 4753 4785 4827 4859 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1518 1486 135 113 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3456 3702 669 Size: 988 281 1518 246 3079 Fragments arranged by size: 3079 1518 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6112 HpaI GTT'AAC Cuts at: 3702 3702 Size: 6112 KasI G'GCGC_C Cuts at: 3318 3318 Size: 6112 KpnI G_GTAC'C Cuts at: 2936 3437 2936 Size: 501 5611 MfeI C'AATT_G Cuts at: 3711 3711 Size: 6112 MluI A'CGCG_T Cuts at: 5988 5988 Size: 6112 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2105 3416 5226 5410 1305 Size: 800 1311 1810 184 2007 Fragments arranged by size: 2007 1810 1311 800 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4290 Fragments arranged by size: 4290 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2544 2927 3243 4070 4429 4588 518 Size: 648 1378 383 316 827 359 159 2042 Fragments arranged by size: 2042 1378 827 648 383 359 316 159 MspA1I CmG'CkG Cuts at: 1606 2450 2582 3171 3528 4205 5353 5598 1606 Size: 844 132 589 357 677 1148 245 2120 Fragments arranged by size: 2120 1148 844 677 589 357 245 132 NarI GG'CG_CC Cuts at: 3319 3319 Size: 6112 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4621 Fragments arranged by size: 4621 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6112 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6112 NotI GC'GGCC_GC Cuts at: 3559 3559 Size: 6112 NspI r_CATG'y Cuts at: 1132 1229 3314 3470 5015 1132 Size: 97 2085 156 1545 2229 Fragments arranged by size: 2229 2085 1545 156 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6112 PciI A'CATG_T Cuts at: 5011 5011 Size: 6112 PflMI CCAn_nnn'nTGG Cuts at: 1108 3098 1108 Size: 1990 4122 PfoI T'CCnGG_A Cuts at: 2057 2206 2057 Size: 149 5963 PmeI GTTT'AAAC Cuts at: 3416 3416 Size: 6112 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4181 4213 5694 5726 4181 Size: 32 1481 32 4567 Fragments arranged by size: 4567 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2834 1613 Size: 1221 4891 PsiI TTA'TAA Cuts at: 3682 3682 Size: 6112 PstI C_TGCA'G Cuts at: 830 2074 2123 2696 2755 2764 3464 830 Size: 1244 49 573 59 9 700 3478 Fragments arranged by size: 3478 1244 700 573 59 49 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6112 PvuII CAG'CTG Cuts at: 2450 3171 2450 Size: 721 5391 SalI G'TCGA_C Cuts at: 1936 3454 1936 Size: 1518 4594 SbfI CC_TGCA'GG Cuts at: 3464 3464 Size: 6112 ScaI AGT'ACT Cuts at: 4360 6072 4360 Size: 1712 4400 SfcI C'TryA_G Cuts at: 826 1045 2070 2094 2119 2692 2751 2760 3082 Size: 219 1025 24 25 573 59 9 322 Cuts at: 3082 3172 3460 4595 5276 5467 826 Size: 90 288 1135 681 191 1471 Fragments arranged by size: 1471 1135 1025 681 573 322 288 219 191 90 59 25 24 9 SfoI GGC'GCC Cuts at: 3320 3320 Size: 6112 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6112 SmaI CCC'GGG Cuts at: 1872 3439 1872 Size: 1567 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2283 4216 5117 5379 820 Size: 197 926 340 1933 901 262 1553 Fragments arranged by size: 1933 1553 926 901 340 262 197 SnaBI TAC'GTA Cuts at: 493 3903 493 Size: 3410 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6112 SphI G_CATG'C Cuts at: 1132 3314 3470 1132 Size: 2182 156 3774 Fragments arranged by size: 3774 2182 156 SspI AAT'ATT Cuts at: 5 52 4036 5 Size: 47 3984 2081 Fragments arranged by size: 3984 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6112 StyI C'CwwG_G Cuts at: 513 1065 2004 3551 513 Size: 552 939 1547 3074 Fragments arranged by size: 3074 1547 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3105 4139 4156 4309 4494 4939 325 Size: 1255 1525 1034 17 153 185 445 1498 Fragments arranged by size: 1525 1498 1255 1034 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2320 4358 6070 Size: 274 80 33 51 1443 343 2038 1712 Cuts at: 6070 96 Size: 138 Fragments arranged by size: 2038 1712 1443 343 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3172 3204 6072 6104 3172 Size: 32 2868 32 3180 Fragments arranged by size: 3180 2868 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6112 XbaI T'CTAG_A Cuts at: 3448 3448 Size: 6112 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3095 3095 Size: 6112 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6112 XmaI C'CCGG_G Cuts at: 1870 3437 1870 Size: 1567 4545 XmnI GAAnn'nnTTC Cuts at: 4241 4241 Size: 6112 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3920 276 Size: 53 83 186 936 2386 2468 Fragments arranged by size: 2468 2386 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI