(Circular) (Six-base) MAPSORT of: pFN21AB3975.seq Check: 7631 from: 1 to: 6592 pFN21AB3975 6592 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3383 3383 Size: 6592 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4402 278 Size: 53 83 186 936 2866 2468 Fragments arranged by size: 2866 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2190 2961 3935 1937 Size: 253 771 974 4594 Fragments arranged by size: 4594 974 771 253 Acc65I G'GTAC_C Cuts at: 3913 3913 Size: 6592 AclI AA'CG_TT Cuts at: 1563 4719 5092 1563 Size: 3156 373 3063 Fragments arranged by size: 3156 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2552 2926 4655 6039 6307 1632 Size: 920 374 1729 1384 268 1917 Fragments arranged by size: 1917 1729 1384 920 374 268 AfeI AGC'GCT Cuts at: 2321 2333 2321 Size: 12 6580 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6395 AflIII A'CryG_T Cuts at: 1203 2889 5491 6468 1203 Size: 1686 2602 977 1327 Fragments arranged by size: 2602 1686 1327 977 AgeI A'CCGG_T Cuts at: 5423 5423 Size: 6592 AhdI GACnn_n'nnGTC Cuts at: 2893 3798 5321 2893 Size: 905 1523 4164 Fragments arranged by size: 4164 1523 905 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6592 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2693 3783 1184 Size: 1509 1090 3993 Fragments arranged by size: 3993 1509 1090 AlwNI CAG_nnn'CTG Cuts at: 2793 3223 3541 3749 4335 5907 2793 Size: 430 318 208 586 1572 3478 Fragments arranged by size: 3478 1572 586 430 318 208 ApaI G_GGCC'C Cuts at: 2052 2218 3113 3162 3332 3482 3489 3625 2052 Size: 166 895 49 170 150 7 136 5019 Fragments arranged by size: 5019 895 170 166 150 136 49 7 ApaLI G'TGCA_C Cuts at: 4649 5805 4649 Size: 1156 5436 ApoI r'AATT_y Cuts at: 1493 3901 4127 4279 6388 1493 Size: 2408 226 152 2109 1697 Fragments arranged by size: 2408 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5146 160 Size: 872 4114 1606 Fragments arranged by size: 4114 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6592 AvaI C'yCGr_G Cuts at: 1870 1943 3113 3161 3917 1870 Size: 73 1170 48 756 4545 Fragments arranged by size: 4545 1170 756 73 48 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3637 3670 3637 Size: 33 6559 BamHI G'GATC_C Cuts at: 3922 4289 3922 Size: 367 6225 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2224 3022 3913 5368 618 Size: 325 217 627 437 798 891 1455 1842 Fragments arranged by size: 1842 1455 891 798 627 437 325 217 BanII G_rGCy'C Cuts at: 1391 2052 2218 2703 2937 3113 3162 3252 3280 Size: 661 166 485 234 176 49 90 28 Cuts at: 3280 3332 3482 3489 3625 3911 1391 Size: 52 150 7 136 286 4072 Fragments arranged by size: 4072 661 485 286 234 176 166 150 136 90 52 49 28 7 BbeI G_GCGC'C Cuts at: 2228 3026 2228 Size: 798 5794 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5621 BbvCI CC'TCA_GC Cuts at: 2128 3772 2128 Size: 1644 4948 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3870 3904 4783 4817 1903 Size: 34 1933 34 879 34 3678 Fragments arranged by size: 3678 1933 879 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4484 5694 4484 Size: 1210 5382 BclI T'GATC_A Cuts at: 1548 2392 1548 Size: 844 5748 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2098 2222 2802 Size: 107 122 71 631 605 426 124 580 Cuts at: 2802 3314 3336 3544 3711 5203 136 Size: 512 22 208 167 1492 1525 Fragments arranged by size: 1525 1492 631 605 580 512 426 208 167 124 122 107 71 22 BglII A'GATC_T Cuts at: 6587 6587 Size: 6592 BlpI GC'TnA_GC Cuts at: 2702 4009 2702 Size: 1307 5285 Bme1580I G_kGCm'C Cuts at: 1790 2052 2218 2867 3113 3162 3332 3482 3489 Size: 262 166 649 246 49 170 150 7 Cuts at: 3489 3570 3625 4653 5809 1790 Size: 81 55 1028 1156 2573 Fragments arranged by size: 2573 1156 1028 649 262 246 170 166 150 81 55 49 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6592 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3619 4323 5281 Size: 416 53 83 399 24 2202 704 958 Cuts at: 5281 442 Size: 1753 Fragments arranged by size: 2202 1753 958 704 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6592 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2954 2954 Size: 6592 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2348 2501 3260 3391 3406 3418 3435 3816 Size: 1109 153 759 131 15 12 17 381 Cuts at: 3816 5252 1239 Size: 1436 2579 Fragments arranged by size: 2579 1436 1109 759 381 153 131 17 15 12 Bpu10I CC'TnA_GC Cuts at: 2128 2697 3772 2128 Size: 569 1075 4948 Fragments arranged by size: 4948 1075 569 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2708 4717 5582 5880 2708 Size: 2009 865 298 3420 Fragments arranged by size: 3420 2009 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5255 882 Size: 617 3756 2219 Fragments arranged by size: 3756 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4383 493 Size: 711 3179 2702 Fragments arranged by size: 3179 2702 711 BsaBI GATnn'nnATC Cuts at: 6586 6586 Size: 6592 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2225 2628 3023 4399 Size: 53 83 186 936 692 403 395 1376 Cuts at: 4399 4781 5416 275 Size: 382 635 1451 Fragments arranged by size: 1451 1376 936 692 635 403 395 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2550 2927 3444 3780 5025 5423 5697 5844 6255 Size: 377 517 336 1245 398 274 147 411 Cuts at: 6255 2550 Size: 2887 Fragments arranged by size: 2887 1245 517 411 398 377 336 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2498 2528 1178 Size: 30 165 30 1095 30 5242 Fragments arranged by size: 5242 1095 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3421 1186 Size: 2235 4357 BseYI C'CCAG_C Cuts at: 2217 2989 3139 3534 3636 3742 3756 5795 6291 Size: 772 150 395 102 106 14 2039 496 Cuts at: 6291 2217 Size: 2518 Fragments arranged by size: 2518 2039 772 496 395 150 106 102 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2653 3167 1163 Size: 573 917 514 4588 Fragments arranged by size: 4588 917 573 514 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3006 3309 4042 4803 5831 1540 Size: 275 186 1005 303 733 761 1028 2301 Fragments arranged by size: 2301 1028 1005 761 733 303 275 186 BsiHKAI G_wGCw'C Cuts at: 2061 2703 2859 3878 4653 4738 5809 2061 Size: 642 156 1019 775 85 1071 2844 Fragments arranged by size: 2844 1071 1019 775 642 156 85 BsmI GAATG_Cn' Cuts at: 4103 4196 4103 Size: 93 6499 BsmBI CGTCTCn'nnnn_ Cuts at: 2635 2635 Size: 6592 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2061 2218 2703 2859 2867 2937 Size: 399 262 9 157 485 156 8 70 Cuts at: 2937 3113 3162 3252 3280 3332 3482 3489 3570 Size: 176 49 90 28 52 150 7 81 Cuts at: 3570 3625 3878 3911 4653 4738 5809 1391 Size: 55 253 33 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 485 399 262 253 176 157 156 150 90 85 81 70 55 52 49 33 28 9 8 7 BspEI T'CCGG_A Cuts at: 2550 3780 6255 2550 Size: 1230 2475 2887 Fragments arranged by size: 2887 2475 1230 BspHI T'CATG_A Cuts at: 4481 6211 4481 Size: 1730 4862 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2056 3383 3947 844 Size: 1212 1327 564 3489 Fragments arranged by size: 3489 1327 1212 564 BsrBI CCG'CTC Cuts at: 1394 1823 2844 4479 1394 Size: 429 1021 1635 3507 Fragments arranged by size: 3507 1635 1021 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5087 5261 65 Size: 1183 3839 174 1396 Fragments arranged by size: 3839 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3001 3168 5236 5423 1700 Size: 110 1191 167 2068 187 2869 Fragments arranged by size: 2869 2068 1191 187 167 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6592 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6592 BssSI C'ACGA_G Cuts at: 4652 5664 4652 Size: 1012 5580 BstAPI GCAn_nnn'nTGC Cuts at: 794 2071 2793 3136 794 Size: 1277 722 343 4250 Fragments arranged by size: 4250 1277 722 343 BstBI TT'CG_AA Cuts at: 5396 5396 Size: 6592 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6592 BstYI r'GATC_y Cuts at: 1972 2811 3922 4289 4674 4691 6143 6229 6241 Size: 839 1111 367 385 17 1452 86 12 Cuts at: 6241 6587 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1111 839 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2191 2191 Size: 6592 Bsu36I CC'TnA_GG Cuts at: 2850 2850 Size: 6592 BtgI C'CryG_G Cuts at: 513 1065 2004 2023 2272 2623 3859 513 Size: 552 939 19 249 351 1236 3246 Fragments arranged by size: 3246 1236 939 552 351 249 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5853 Fragments arranged by size: 5853 683 56 BtsI GCAGTG_nn' Cuts at: 2317 2675 2713 3349 3605 4108 4902 4922 2317 Size: 358 38 636 256 503 794 20 3987 Fragments arranged by size: 3987 794 636 503 358 256 38 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6592 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6557 DraI TTT'AAA Cuts at: 3896 3896 Size: 6592 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5599 809 Size: 507 4283 1802 Fragments arranged by size: 4283 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2371 2415 Size: 54 1425 50 275 18 177 364 44 Cuts at: 2415 2688 2803 2880 3003 3306 4039 4928 6557 Size: 273 115 77 123 303 733 889 1629 Cuts at: 6557 8 Size: 43 Fragments arranged by size: 1629 1425 889 733 364 303 275 273 177 123 115 77 54 50 44 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3003 3306 4039 1537 Size: 275 1191 303 733 4090 Fragments arranged by size: 4090 1191 733 303 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2945 3388 4522 1353 Size: 1592 443 1134 3423 Fragments arranged by size: 3423 1592 1134 443 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3287 5179 5553 5699 387 Size: 1064 138 1698 1892 374 146 1280 Fragments arranged by size: 1892 1698 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2701 2701 Size: 6592 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2348 2501 2552 2926 3260 3391 3406 Size: 393 716 153 51 374 334 131 15 Cuts at: 3406 3418 3435 3816 4655 5252 6039 6307 1239 Size: 12 17 381 839 597 787 268 1524 Fragments arranged by size: 1524 839 787 716 597 393 381 374 334 268 153 131 51 17 15 12 EcoNI CCTnn'n_nnAGG Cuts at: 2453 2453 Size: 6592 EcoO109I rG'GnC_Cy Cuts at: 2048 2049 2263 3109 3110 3220 3478 3479 3486 Size: 1 214 846 1 110 258 1 7 Cuts at: 3486 3523 3621 3842 2048 Size: 37 98 221 4798 Fragments arranged by size: 4798 846 258 221 214 110 98 37 7 1 1 1 EcoRI G'AATT_C Cuts at: 3901 4279 3901 Size: 378 6214 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2557 2557 Size: 6592 FspI TGC'GCA Cuts at: 2309 2609 3233 5098 2309 Size: 300 624 1865 3803 Fragments arranged by size: 3803 1865 624 300 HaeII r_GCGC'y Cuts at: 1678 2228 2323 2335 2766 3026 3749 5739 1678 Size: 550 95 12 431 260 723 1990 2531 Fragments arranged by size: 2531 1990 723 550 431 260 95 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2111 2143 2567 2599 2879 Size: 32 135 32 539 32 424 32 280 Cuts at: 2879 2911 3784 3816 5233 5265 5307 5339 1373 Size: 32 873 32 1417 32 42 32 2626 Fragments arranged by size: 2626 1417 873 539 424 280 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2962 3936 4182 669 Size: 988 281 1024 974 246 3079 Fragments arranged by size: 3079 1024 988 974 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6592 HpaI GTT'AAC Cuts at: 4182 4182 Size: 6592 KasI G'GCGC_C Cuts at: 2224 3022 2224 Size: 798 5794 KpnI G_GTAC'C Cuts at: 3917 3917 Size: 6592 MfeI C'AATT_G Cuts at: 4191 4191 Size: 6592 MluI A'CGCG_T Cuts at: 6468 6468 Size: 6592 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5706 5890 1305 Size: 4401 184 2007 Fragments arranged by size: 4401 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2373 2417 2690 2805 2882 Size: 54 1425 343 541 44 273 115 77 Cuts at: 2882 10 Size: 3720 Fragments arranged by size: 3720 1425 541 343 273 115 77 54 44 MslI CAynn'nnrTG Cuts at: 518 1166 2181 2277 2507 2888 2912 4550 4909 Size: 648 1015 96 230 381 24 1638 359 Cuts at: 4909 5068 518 Size: 159 2042 Fragments arranged by size: 2042 1638 1015 648 381 359 230 159 96 24 MspA1I CmG'CkG Cuts at: 1606 2137 2625 3541 4008 4685 5833 6078 1606 Size: 531 488 916 467 677 1148 245 2120 Fragments arranged by size: 2120 1148 916 677 531 488 467 245 NaeI GCC'GGC Cuts at: 3170 3170 Size: 6592 NarI GG'CG_CC Cuts at: 2225 3023 2225 Size: 798 5794 NcoI C'CATG_G Cuts at: 513 1065 2004 2023 2272 513 Size: 552 939 19 249 4833 Fragments arranged by size: 4833 939 552 249 19 NdeI CA'TA_TG Cuts at: 387 387 Size: 6592 NgoMIV G'CCGG_C Cuts at: 3168 3168 Size: 6592 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6592 NotI GC'GGCC_GC Cuts at: 4039 4039 Size: 6592 NspI r_CATG'y Cuts at: 1132 1229 2893 3238 3950 5495 1132 Size: 97 1664 345 712 1545 2229 Fragments arranged by size: 2229 1664 1545 712 345 97 PasI CC'CwG_GG Cuts at: 1348 2045 2899 3271 3482 3549 1348 Size: 697 854 372 211 67 4391 Fragments arranged by size: 4391 854 697 372 211 67 PciI A'CATG_T Cuts at: 2889 5491 2889 Size: 2602 3990 PflMI CCAn_nnn'nTGG Cuts at: 1108 3223 1108 Size: 2115 4477 PfoI T'CCnGG_A Cuts at: 2115 3216 2115 Size: 1101 5491 PmeI GTTT'AAAC Cuts at: 3896 3896 Size: 6592 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4661 4693 6174 6206 4661 Size: 32 1481 32 5047 Fragments arranged by size: 5047 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2263 3220 3523 2263 Size: 957 303 5332 Fragments arranged by size: 5332 957 303 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6592 PsiI TTA'TAA Cuts at: 4162 4162 Size: 6592 PspOMI G'GGCC_C Cuts at: 2048 2214 3109 3158 3328 3478 3485 3621 2048 Size: 166 895 49 170 150 7 136 5019 Fragments arranged by size: 5019 895 170 166 150 136 49 7 PstI C_TGCA'G Cuts at: 830 3132 3285 3944 830 Size: 2302 153 659 3478 Fragments arranged by size: 3478 2302 659 153 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6592 SacI G_AGCT'C Cuts at: 2703 2703 Size: 6592 SacII CC_GC'GG Cuts at: 2626 2626 Size: 6592 SalI G'TCGA_C Cuts at: 1936 2960 3934 1936 Size: 1024 974 4594 Fragments arranged by size: 4594 1024 974 SbfI CC_TGCA'GG Cuts at: 3944 3944 Size: 6592 ScaI AGT'ACT Cuts at: 2516 3041 4840 6552 2516 Size: 525 1799 1712 2556 Fragments arranged by size: 2556 1799 1712 525 SexAI A'CCwGG_T Cuts at: 3222 3222 Size: 6592 SfcI C'TryA_G Cuts at: 826 1045 3128 3281 3457 3940 5075 5756 5947 Size: 219 2083 153 176 483 1135 681 191 Cuts at: 5947 826 Size: 1471 Fragments arranged by size: 2083 1471 1135 681 483 219 191 176 153 SfiI GGCCn_nnn'nGGCC Cuts at: 3314 3314 Size: 6592 SfoI GGC'GCC Cuts at: 2226 3024 2226 Size: 798 5794 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6592 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6592 SmaI CCC'GGG Cuts at: 1872 3115 3163 3919 1872 Size: 1243 48 756 4545 Fragments arranged by size: 4545 1243 756 48 SmlI C'TyrA_G Cuts at: 820 1017 1943 2723 4696 5597 5859 820 Size: 197 926 780 1973 901 262 1553 Fragments arranged by size: 1973 1553 926 901 780 262 197 SnaBI TAC'GTA Cuts at: 493 4383 493 Size: 3890 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6592 SphI G_CATG'C Cuts at: 1132 3238 3950 1132 Size: 2106 712 3774 Fragments arranged by size: 3774 2106 712 SspI AAT'ATT Cuts at: 5 52 4516 5 Size: 47 4464 2081 Fragments arranged by size: 4464 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6592 StyI C'CwwG_G Cuts at: 513 1065 2004 2023 2272 3027 3649 4031 513 Size: 552 939 19 249 755 622 382 3074 Fragments arranged by size: 3074 939 755 622 552 382 249 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2630 3756 4619 4636 4789 4974 5419 Size: 1255 1050 1126 863 17 153 185 445 Cuts at: 5419 325 Size: 1498 Fragments arranged by size: 1498 1255 1126 1050 863 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2514 2524 3039 Size: 274 80 33 51 1443 537 10 515 Cuts at: 3039 4838 6550 96 Size: 1799 1712 138 Fragments arranged by size: 1799 1712 1443 537 515 274 138 80 51 33 10 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6552 6584 6552 Size: 32 6560 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6592 XbaI T'CTAG_A Cuts at: 3928 3928 Size: 6592 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2773 2773 Size: 6592 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6592 XmaI C'CCGG_G Cuts at: 1870 3113 3161 3917 1870 Size: 1243 48 756 4545 Fragments arranged by size: 4545 1243 756 48 XmnI GAAnn'nnTTC Cuts at: 4721 4721 Size: 6592 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4400 276 Size: 53 83 186 936 2866 2468 Fragments arranged by size: 2866 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BfrBI BsiWI BstXI DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SanDI SapI SrfI SwaI