(Circular) (Six-base) MAPSORT of: pFN21AB3946.seq Check: 9855 from: 1 to: 6232 pFN21AB3946 6232 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2850 4042 278 Size: 53 83 186 936 1314 1192 2468 Fragments arranged by size: 2468 1314 1192 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3510 3575 1937 Size: 1573 65 4594 Fragments arranged by size: 4594 1573 65 Acc65I G'GTAC_C Cuts at: 3553 3553 Size: 6232 AclI AA'CG_TT Cuts at: 1563 4359 4732 1563 Size: 2796 373 3063 Fragments arranged by size: 3063 2796 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2204 2780 4295 5679 5947 1632 Size: 572 576 1515 1384 268 1917 Fragments arranged by size: 1917 1515 1384 576 572 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6035 AflIII A'CryG_T Cuts at: 1203 2157 3003 5131 6108 1203 Size: 954 846 2128 977 1327 Fragments arranged by size: 2128 1327 977 954 846 AgeI A'CCGG_T Cuts at: 5063 5063 Size: 6232 AhdI GACnn_n'nnGTC Cuts at: 4961 4961 Size: 6232 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6232 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6232 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2144 2176 2192 2224 3335 3367 2144 Size: 32 16 32 1111 32 5009 Fragments arranged by size: 5009 1111 32 32 32 16 AlwNI CAG_nnn'CTG Cuts at: 2896 3975 5547 2896 Size: 1079 1572 3581 Fragments arranged by size: 3581 1572 1079 ApaI G_GGCC'C Cuts at: 2705 2705 Size: 6232 ApaLI G'TGCA_C Cuts at: 2666 4289 5445 2666 Size: 1623 1156 3453 Fragments arranged by size: 3453 1623 1156 ApoI r'AATT_y Cuts at: 1493 3541 3767 3919 6028 1493 Size: 2048 226 152 2109 1697 Fragments arranged by size: 2109 2048 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4786 160 Size: 872 3754 1606 Fragments arranged by size: 3754 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 AvaI C'yCGr_G Cuts at: 1870 1943 2686 2914 3557 1870 Size: 73 743 228 643 4545 Fragments arranged by size: 4545 743 643 228 73 BamHI G'GATC_C Cuts at: 3562 3929 3562 Size: 367 5865 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2063 2269 2772 2920 3025 Size: 325 217 627 276 206 503 148 105 Cuts at: 3025 3076 3097 3118 3380 3553 5008 618 Size: 51 21 21 262 173 1455 1842 Fragments arranged by size: 1842 1455 627 503 325 276 262 217 206 173 148 105 51 21 21 BanII G_rGCy'C Cuts at: 1391 2102 2120 2178 2409 2705 3215 3551 1391 Size: 711 18 58 231 296 510 336 4072 Fragments arranged by size: 4072 711 510 336 296 231 58 18 BbeI G_GCGC'C Cuts at: 2067 2776 3029 3080 3101 2067 Size: 709 253 51 21 5198 Fragments arranged by size: 5198 709 253 51 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5261 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2353 2387 4423 4457 1903 Size: 34 416 34 2036 34 3678 Fragments arranged by size: 3678 2036 416 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4124 5334 4124 Size: 1210 5022 BclI T'GATC_A Cuts at: 1548 1548 Size: 6232 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2061 2115 2987 Size: 107 122 71 631 605 389 54 872 Cuts at: 2987 3065 3074 4843 136 Size: 78 9 1769 1525 Fragments arranged by size: 1769 1525 872 631 605 389 122 107 78 71 54 9 BglII A'GATC_T Cuts at: 6227 6227 Size: 6232 BlpI GC'TnA_GC Cuts at: 2408 2837 3158 3209 3420 3649 2408 Size: 429 321 51 211 229 4991 Fragments arranged by size: 4991 429 321 229 211 51 Bme1580I G_kGCm'C Cuts at: 1790 2274 2670 2705 3383 4293 5449 1790 Size: 484 396 35 678 910 1156 2573 Fragments arranged by size: 2573 1156 910 678 484 396 35 BmgBI CAC'GTC Cuts at: 1318 2317 2737 2821 1318 Size: 999 420 84 4729 Fragments arranged by size: 4729 999 420 84 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3505 3963 4921 Size: 416 53 83 399 24 2088 458 958 Cuts at: 4921 442 Size: 1753 Fragments arranged by size: 2088 1753 958 458 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6232 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2686 2686 Size: 6232 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2281 2452 3331 4892 1239 Size: 1042 171 879 1561 2579 Fragments arranged by size: 2579 1561 1042 879 171 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4357 5222 5520 4357 Size: 865 298 5069 Fragments arranged by size: 5069 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2858 4895 882 Size: 617 1359 2037 2219 Fragments arranged by size: 2219 2037 1359 617 BsaAI yAC'GTr Cuts at: 493 1204 3004 4023 493 Size: 711 1800 1019 2702 Fragments arranged by size: 2702 1800 1019 711 BsaBI GATnn'nnATC Cuts at: 6226 6226 Size: 6232 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2064 2773 2847 3026 Size: 53 83 186 936 531 709 74 179 Cuts at: 3026 3077 3098 4039 4421 5056 275 Size: 51 21 941 382 635 1451 Fragments arranged by size: 1451 941 936 709 635 531 382 186 179 83 74 53 51 21 BsaWI w'CCGG_w Cuts at: 4665 5063 5337 5484 5895 4665 Size: 398 274 147 411 5002 Fragments arranged by size: 5002 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2144 2174 2192 2222 2633 Size: 30 165 30 741 30 18 30 411 Cuts at: 2633 2663 2837 2867 3278 3308 3329 3335 3359 Size: 30 174 30 411 30 21 6 24 Cuts at: 3359 3365 1178 Size: 6 4045 Fragments arranged by size: 4045 741 411 411 174 165 30 30 30 30 30 30 30 24 21 18 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2213 2486 2588 2635 2638 1186 Size: 1027 273 102 47 3 4780 Fragments arranged by size: 4780 1027 273 102 47 3 BseYI C'CCAG_C Cuts at: 2069 2419 3180 3215 3489 5435 5931 2069 Size: 350 761 35 274 1946 496 2370 Fragments arranged by size: 2370 1946 761 496 350 274 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2513 2759 1163 Size: 573 777 246 4636 Fragments arranged by size: 4636 777 573 246 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3682 4443 5471 1540 Size: 275 186 1681 761 1028 2301 Fragments arranged by size: 2301 1681 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2247 2409 2670 2685 4293 4378 5449 2247 Size: 162 261 15 1608 85 1071 3030 Fragments arranged by size: 3030 1608 1071 261 162 85 15 BsmI GAATG_Cn' Cuts at: 3743 3836 3743 Size: 93 6139 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2102 2120 2178 2247 2274 2409 2670 Size: 399 312 18 58 69 27 135 261 Cuts at: 2670 2685 2705 3215 3383 3551 4293 4378 5449 Size: 15 20 510 168 168 742 85 1071 Cuts at: 5449 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 510 399 312 261 168 168 135 85 69 58 27 20 18 15 BspEI T'CCGG_A Cuts at: 5895 5895 Size: 6232 BspHI T'CATG_A Cuts at: 2025 4121 5851 2025 Size: 2096 1730 2406 Fragments arranged by size: 2406 2096 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2904 3587 844 Size: 2060 683 3489 Fragments arranged by size: 3489 2060 683 BsrBI CCG'CTC Cuts at: 1394 1823 2482 3315 3419 4119 1394 Size: 429 659 833 104 700 3507 Fragments arranged by size: 3507 833 700 659 429 104 BsrDI GCAATG_nn' Cuts at: 65 1248 4727 4901 65 Size: 1183 3479 174 1396 Fragments arranged by size: 3479 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2278 2923 3064 3112 3383 4876 5063 Size: 110 468 645 141 48 271 1493 187 Cuts at: 5063 1700 Size: 2869 Fragments arranged by size: 2869 1493 645 468 271 187 141 110 48 BsrGI T'GTAC_A Cuts at: 96 3432 96 Size: 3336 2896 BssHII G'CGCG_C Cuts at: 1925 2360 1925 Size: 435 5797 BssSI C'ACGA_G Cuts at: 4292 5304 4292 Size: 1012 5220 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6232 BstBI TT'CG_AA Cuts at: 5036 5036 Size: 6232 BstEII G'GTnAC_C Cuts at: 1184 2941 1184 Size: 1757 4475 BstYI r'GATC_y Cuts at: 1972 3562 3929 4314 4331 5783 5869 5881 6227 Size: 1590 367 385 17 1452 86 12 346 Cuts at: 6227 1972 Size: 1977 Fragments arranged by size: 1977 1590 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2094 2531 2580 2976 513 Size: 552 1029 437 49 396 3769 Fragments arranged by size: 3769 1029 552 437 396 49 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3427 487 Size: 683 56 2201 3292 Fragments arranged by size: 3292 2201 683 56 BtsI GCAGTG_nn' Cuts at: 3748 4542 4562 3748 Size: 794 20 5418 Fragments arranged by size: 5418 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6232 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6197 DraI TTT'AAA Cuts at: 3536 3536 Size: 6232 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5239 809 Size: 507 3923 1802 Fragments arranged by size: 3923 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2908 2925 2935 Size: 54 1425 50 275 18 1078 17 10 Cuts at: 2935 2979 3679 4568 6197 8 Size: 44 700 889 1629 43 Fragments arranged by size: 1629 1425 1078 889 700 275 54 50 44 43 18 17 10 EagI C'GGCC_G Cuts at: 1537 1812 3679 1537 Size: 275 1867 4090 Fragments arranged by size: 4090 1867 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4162 1353 Size: 2809 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2492 2551 2579 4819 5193 5339 Size: 1064 138 903 59 28 2240 374 146 Cuts at: 5339 387 Size: 1280 Fragments arranged by size: 2240 1280 1064 903 374 146 138 59 28 EcoICRI GAG'CTC Cuts at: 2407 2407 Size: 6232 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2204 2281 2452 2780 3331 4295 4892 Size: 393 572 77 171 328 551 964 597 Cuts at: 4892 5679 5947 1239 Size: 787 268 1524 Fragments arranged by size: 1524 964 787 597 572 551 393 328 268 171 77 EcoO109I rG'GnC_Cy Cuts at: 2053 2701 3058 3460 2053 Size: 648 357 402 4825 Fragments arranged by size: 4825 648 402 357 EcoRI G'AATT_C Cuts at: 3541 3919 3541 Size: 378 5854 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2479 2479 Size: 6232 FspI TGC'GCA Cuts at: 4738 4738 Size: 6232 HaeII r_GCGC'y Cuts at: 1678 2067 2624 2776 3029 3080 3101 3427 5379 Size: 389 557 152 253 51 21 326 1952 Cuts at: 5379 1678 Size: 2531 Fragments arranged by size: 2531 1952 557 389 326 253 152 51 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2837 2869 4873 4905 4947 Size: 32 135 32 1265 32 2004 32 42 Cuts at: 4947 4979 1373 Size: 32 2626 Fragments arranged by size: 2626 2004 1265 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2290 2878 3576 3822 669 Size: 988 281 352 588 698 246 3079 Fragments arranged by size: 3079 988 698 588 352 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6232 HpaI GTT'AAC Cuts at: 3822 3822 Size: 6232 KasI G'GCGC_C Cuts at: 2063 2772 3025 3076 3097 2063 Size: 709 253 51 21 5198 Fragments arranged by size: 5198 709 253 51 21 KpnI G_GTAC'C Cuts at: 3557 3557 Size: 6232 MfeI C'AATT_G Cuts at: 3831 3831 Size: 6232 MluI A'CGCG_T Cuts at: 6108 6108 Size: 6232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2880 5346 5530 1305 Size: 1575 2466 184 2007 Fragments arranged by size: 2466 2007 1575 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4410 Fragments arranged by size: 4410 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2030 2279 3407 4190 4549 4708 518 Size: 648 864 249 1128 783 359 159 2042 Fragments arranged by size: 2042 1128 864 783 648 359 249 159 MspA1I CmG'CkG Cuts at: 1606 2096 2533 2978 3208 3648 4325 5473 5718 Size: 490 437 445 230 440 677 1148 245 Cuts at: 5718 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 490 445 440 437 245 230 NaeI GCC'GGC Cuts at: 2925 3066 3114 2925 Size: 141 48 6043 Fragments arranged by size: 6043 141 48 NarI GG'CG_CC Cuts at: 2064 2773 3026 3077 3098 2064 Size: 709 253 51 21 5198 Fragments arranged by size: 5198 709 253 51 21 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5680 NdeI CA'TA_TG Cuts at: 387 387 Size: 6232 NgoMIV G'CCGG_C Cuts at: 2923 3064 3112 2923 Size: 141 48 6043 Fragments arranged by size: 6043 141 48 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6232 NotI GC'GGCC_GC Cuts at: 3679 3679 Size: 6232 NspI r_CATG'y Cuts at: 1132 1229 3590 5135 1132 Size: 97 2361 1545 2229 Fragments arranged by size: 2361 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2698 1348 Size: 1350 4882 PciI A'CATG_T Cuts at: 5131 5131 Size: 6232 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6232 PfoI T'CCnGG_A Cuts at: 2013 2013 Size: 6232 PmeI GTTT'AAAC Cuts at: 3536 3536 Size: 6232 PmlI CAC'GTG Cuts at: 3004 3004 Size: 6232 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2144 2176 2192 2224 3335 3367 4301 4333 5814 Size: 32 16 32 1111 32 934 32 1481 Cuts at: 5814 5846 2144 Size: 32 2530 Fragments arranged by size: 2530 1481 1111 934 32 32 32 32 32 16 PpuMI rG'GwC_Cy Cuts at: 3460 3460 Size: 6232 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6232 PsiI TTA'TAA Cuts at: 3802 3802 Size: 6232 PspOMI G'GGCC_C Cuts at: 2701 2701 Size: 6232 PstI C_TGCA'G Cuts at: 830 2628 3584 830 Size: 1798 956 3478 Fragments arranged by size: 3478 1798 956 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6232 PvuII CAG'CTG Cuts at: 3208 3208 Size: 6232 SacI G_AGCT'C Cuts at: 2409 2409 Size: 6232 SacII CC_GC'GG Cuts at: 2097 2534 2979 2097 Size: 437 445 5350 Fragments arranged by size: 5350 445 437 SalI G'TCGA_C Cuts at: 1936 3574 1936 Size: 1638 4594 SanDI GG'GwC_CC Cuts at: 3460 3460 Size: 6232 SbfI CC_TGCA'GG Cuts at: 3584 3584 Size: 6232 ScaI AGT'ACT Cuts at: 4480 6192 4480 Size: 1712 4520 SfcI C'TryA_G Cuts at: 826 1045 2624 3223 3262 3286 3358 3580 4715 Size: 219 1579 599 39 24 72 222 1135 Cuts at: 4715 5396 5587 826 Size: 681 191 1471 Fragments arranged by size: 1579 1471 1135 681 599 222 219 191 72 39 24 SfiI GGCCn_nnn'nGGCC Cuts at: 3065 3065 Size: 6232 SfoI GGC'GCC Cuts at: 2065 2774 3027 3078 3099 2065 Size: 709 253 51 21 5198 Fragments arranged by size: 5198 709 253 51 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6232 SmaI CCC'GGG Cuts at: 1872 2916 3559 1872 Size: 1044 643 4545 Fragments arranged by size: 4545 1044 643 SmlI C'TyrA_G Cuts at: 820 1017 1943 4336 5237 5499 820 Size: 197 926 2393 901 262 1553 Fragments arranged by size: 2393 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4023 493 Size: 3530 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6232 SphI G_CATG'C Cuts at: 1132 3590 1132 Size: 2458 3774 SspI AAT'ATT Cuts at: 5 52 4156 5 Size: 47 4104 2081 Fragments arranged by size: 4104 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6232 StyI C'CwwG_G Cuts at: 513 1065 2166 3671 513 Size: 552 1101 1505 3074 Fragments arranged by size: 3074 1505 1101 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2051 2731 4259 4276 4429 4614 5059 Size: 1255 471 680 1528 17 153 185 445 Cuts at: 5059 325 Size: 1498 Fragments arranged by size: 1528 1498 1255 680 471 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3432 4478 6190 Size: 274 80 33 51 1443 1455 1046 1712 Cuts at: 6190 96 Size: 138 Fragments arranged by size: 1712 1455 1443 1046 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2633 2665 3329 3361 6192 6224 2633 Size: 32 664 32 2831 32 2641 Fragments arranged by size: 2831 2641 664 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6232 XbaI T'CTAG_A Cuts at: 3568 3568 Size: 6232 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6232 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6232 XmaI C'CCGG_G Cuts at: 1870 2914 3557 1870 Size: 1044 643 4545 Fragments arranged by size: 4545 1044 643 XmnI GAAnn'nnTTC Cuts at: 2159 2336 4361 2159 Size: 177 2025 4030 Fragments arranged by size: 4030 2025 177 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2848 4040 276 Size: 53 83 186 936 1314 1192 2468 Fragments arranged by size: 2468 1314 1192 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SapI SexAI SrfI SwaI