(Circular) (Six-base) MAPSORT of: pFN21AB3902.seq Check: 5369 from: 1 to: 6541 pFN21AB3902 6541 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3832 4351 278 Size: 53 83 186 936 2296 519 2468 Fragments arranged by size: 2468 2296 936 519 186 83 53 AccI GT'mk_AC Cuts at: 1937 2907 3376 3884 1937 Size: 970 469 508 4594 Fragments arranged by size: 4594 970 508 469 Acc65I G'GTAC_C Cuts at: 3862 3862 Size: 6541 AclI AA'CG_TT Cuts at: 1563 4668 5041 1563 Size: 3105 373 3063 Fragments arranged by size: 3105 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4604 5988 6256 1632 Size: 2972 1384 268 1917 Fragments arranged by size: 2972 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6344 AflIII A'CryG_T Cuts at: 1203 2920 3585 5440 6417 1203 Size: 1717 665 1855 977 1327 Fragments arranged by size: 1855 1717 1327 977 665 AgeI A'CCGG_T Cuts at: 5372 5372 Size: 6541 AhdI GACnn_n'nnGTC Cuts at: 2454 5270 2454 Size: 2816 3725 AleI CACnn'nnGTG Cuts at: 1166 2431 1166 Size: 1265 5276 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6541 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3672 3704 3672 Size: 32 6509 AlwNI CAG_nnn'CTG Cuts at: 2452 3028 4284 5856 2452 Size: 576 1256 1572 3137 Fragments arranged by size: 3137 1572 1256 576 ApaI G_GGCC'C Cuts at: 3498 3498 Size: 6541 ApaLI G'TGCA_C Cuts at: 4598 5754 4598 Size: 1156 5385 ApoI r'AATT_y Cuts at: 1493 3386 3850 4076 4228 6337 1493 Size: 1893 464 226 152 2109 1697 Fragments arranged by size: 2109 1893 1697 464 226 152 AseI AT'TA_AT Cuts at: 160 1032 5095 160 Size: 872 4063 1606 Fragments arranged by size: 4063 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6541 AvaI C'yCGr_G Cuts at: 1870 1943 3866 1870 Size: 73 1923 4545 Fragments arranged by size: 4545 1923 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2197 2230 2197 Size: 33 6508 BamHI G'GATC_C Cuts at: 3871 4238 3871 Size: 367 6174 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3862 5317 618 Size: 325 217 627 2075 1455 1842 Fragments arranged by size: 2075 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2087 2307 3201 3498 3860 1391 Size: 696 220 894 297 362 4072 Fragments arranged by size: 4072 894 696 362 297 220 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2267 2450 3291 928 Size: 971 368 183 841 4178 Fragments arranged by size: 4178 971 841 368 183 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4732 4766 1903 Size: 34 2795 34 3678 Fragments arranged by size: 3678 2795 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3310 4433 5643 3310 Size: 1123 1210 4208 Fragments arranged by size: 4208 1210 1123 BclI T'GATC_A Cuts at: 1548 3360 1548 Size: 1812 4729 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5152 136 Size: 107 122 71 631 605 3480 1525 Fragments arranged by size: 3480 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6536 6536 Size: 6541 BlpI GC'TnA_GC Cuts at: 3958 3958 Size: 6541 Bme1580I G_kGCm'C Cuts at: 1790 3161 3498 4602 5758 1790 Size: 1371 337 1104 1156 2573 Fragments arranged by size: 2573 1371 1156 1104 337 BmgBI CAC'GTC Cuts at: 1318 2653 2853 1318 Size: 1335 200 5006 Fragments arranged by size: 5006 1335 200 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2751 3164 3541 Size: 416 53 83 399 24 1334 413 377 Cuts at: 3541 4272 5230 442 Size: 731 958 1753 Fragments arranged by size: 1753 1334 958 731 416 413 399 377 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6541 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3448 3448 Size: 6541 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2954 2975 5201 1239 Size: 1715 21 2226 2579 Fragments arranged by size: 2579 2226 1715 21 Bpu10I CC'TnA_GC Cuts at: 2943 2943 Size: 6541 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2218 3176 4666 5531 5829 2218 Size: 958 1490 865 298 2930 Fragments arranged by size: 2930 1490 958 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5204 882 Size: 617 3705 2219 Fragments arranged by size: 3705 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2921 3588 4332 493 Size: 711 1717 667 744 2702 Fragments arranged by size: 2702 1717 744 711 667 BsaBI GATnn'nnATC Cuts at: 6535 6535 Size: 6541 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3829 4348 4730 5365 Size: 53 83 186 936 2296 519 382 635 Cuts at: 5365 275 Size: 1451 Fragments arranged by size: 2296 1451 936 635 519 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2312 4974 5372 5646 5793 6204 2312 Size: 2662 398 274 147 411 2649 Fragments arranged by size: 2662 2649 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6316 Fragments arranged by size: 6316 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2951 3440 1186 Size: 1765 489 4287 Fragments arranged by size: 4287 1765 489 BseYI C'CCAG_C Cuts at: 2023 2056 2589 5744 6240 2023 Size: 33 533 3155 496 2324 Fragments arranged by size: 3155 2324 533 496 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2987 2996 3431 1163 Size: 573 1251 9 435 4273 Fragments arranged by size: 4273 1251 573 435 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2147 3991 4752 5780 1540 Size: 275 186 146 1844 761 1028 2301 Fragments arranged by size: 2301 1844 1028 761 275 186 146 BsiHKAI G_wGCw'C Cuts at: 2307 2961 3215 4602 4687 5758 2307 Size: 654 254 1387 85 1071 3090 Fragments arranged by size: 3090 1387 1071 654 254 85 BsiWI C'GTAC_G Cuts at: 2665 2665 Size: 6541 BsmI GAATG_Cn' Cuts at: 2355 4052 4145 2355 Size: 1697 93 4751 Fragments arranged by size: 4751 1697 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2307 2961 3161 3201 3215 3498 Size: 399 297 220 654 200 40 14 283 Cuts at: 3498 3860 4602 4687 5758 1391 Size: 362 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 654 399 362 297 283 220 200 85 40 14 BspEI T'CCGG_A Cuts at: 6204 6204 Size: 6541 BspHI T'CATG_A Cuts at: 3357 4430 6160 3357 Size: 1073 1730 3738 Fragments arranged by size: 3738 1730 1073 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3896 844 Size: 3052 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4428 1394 Size: 429 2605 3507 Fragments arranged by size: 3507 2605 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2206 2704 2856 2890 3138 5036 5210 Size: 1183 958 498 152 34 248 1898 174 Cuts at: 5210 65 Size: 1396 Fragments arranged by size: 1898 1396 1183 958 498 248 174 152 34 BsrFI r'CCGG_y Cuts at: 1700 1810 2139 2415 5185 5372 1700 Size: 110 329 276 2770 187 2869 Fragments arranged by size: 2869 2770 329 276 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6541 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6541 BssSI C'ACGA_G Cuts at: 2431 4601 5613 2431 Size: 2170 1012 3359 Fragments arranged by size: 3359 2170 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3010 3028 794 Size: 2216 18 4307 Fragments arranged by size: 4307 2216 18 BstBI TT'CG_AA Cuts at: 5345 5345 Size: 6541 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6541 BstYI r'GATC_y Cuts at: 1972 3871 4238 4623 4640 6092 6178 6190 6536 Size: 1899 367 385 17 1452 86 12 346 Cuts at: 6536 1972 Size: 1977 Fragments arranged by size: 1977 1899 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3684 3684 Size: 6541 BtgI C'CryG_G Cuts at: 513 1065 2004 2292 3405 3460 3784 513 Size: 552 939 288 1113 55 324 3270 Fragments arranged by size: 3270 1113 939 552 324 288 55 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5802 Fragments arranged by size: 5802 683 56 BtsI GCAGTG_nn' Cuts at: 2959 3443 4057 4851 4871 2959 Size: 484 614 794 20 4629 Fragments arranged by size: 4629 794 614 484 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6541 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6506 DraI TTT'AAA Cuts at: 3845 3845 Size: 6541 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2735 3506 3590 5548 809 Size: 507 1419 771 84 1958 1802 Fragments arranged by size: 1958 1802 1419 771 507 84 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2144 3181 3988 Size: 54 1425 50 275 18 314 1037 807 Cuts at: 3988 4877 6506 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1037 889 807 314 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2144 3988 1537 Size: 275 332 1844 4090 Fragments arranged by size: 4090 1844 332 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2168 2447 2606 3101 3356 4471 1353 Size: 815 279 159 495 255 1115 3423 Fragments arranged by size: 3423 1115 815 495 279 255 159 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2059 5128 5502 5648 387 Size: 1064 138 470 3069 374 146 1280 Fragments arranged by size: 3069 1280 1064 470 374 146 138 EcoICRI GAG'CTC Cuts at: 2305 2305 Size: 6541 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2954 2975 4604 5201 5988 6256 1239 Size: 393 1322 21 1629 597 787 268 1524 Fragments arranged by size: 1629 1524 1322 787 597 393 268 21 EcoO109I rG'GnC_Cy Cuts at: 3401 3401 Size: 6541 EcoRI G'AATT_C Cuts at: 3850 4228 3850 Size: 378 6163 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3217 3217 Size: 6541 FspI TGC'GCA Cuts at: 5047 5047 Size: 6541 HaeII r_GCGC'y Cuts at: 1678 5688 1678 Size: 4010 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2440 2472 5182 5214 5256 Size: 32 135 32 868 32 2710 32 42 Cuts at: 5256 5288 1373 Size: 32 2626 Fragments arranged by size: 2710 2626 868 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2656 3377 3885 4131 669 Size: 988 281 718 721 508 246 3079 Fragments arranged by size: 3079 988 721 718 508 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6541 HpaI GTT'AAC Cuts at: 4131 4131 Size: 6541 KpnI G_GTAC'C Cuts at: 3866 3866 Size: 6541 MfeI C'AATT_G Cuts at: 2134 4140 2134 Size: 2006 4535 MluI A'CGCG_T Cuts at: 6417 6417 Size: 6541 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2454 3292 5655 5839 1305 Size: 1149 838 2363 184 2007 Fragments arranged by size: 2363 2007 1149 838 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3183 10 Size: 54 1425 343 1351 3368 Fragments arranged by size: 3368 1425 1351 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2431 3050 3515 4499 4858 5017 518 Size: 648 1265 619 465 984 359 159 2042 Fragments arranged by size: 2042 1265 984 648 619 465 359 159 MspA1I CmG'CkG Cuts at: 1606 2499 2511 3957 4634 5782 6027 1606 Size: 893 12 1446 677 1148 245 2120 Fragments arranged by size: 2120 1446 1148 893 677 245 12 NaeI GCC'GGC Cuts at: 2141 2141 Size: 6541 NcoI C'CATG_G Cuts at: 513 1065 2004 3784 513 Size: 552 939 1780 3270 Fragments arranged by size: 3270 1780 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6541 NgoMIV G'CCGG_C Cuts at: 2139 2139 Size: 6541 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6541 NotI GC'GGCC_GC Cuts at: 3988 3988 Size: 6541 NspI r_CATG'y Cuts at: 1132 1229 3899 5444 1132 Size: 97 2670 1545 2229 Fragments arranged by size: 2670 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6541 PciI A'CATG_T Cuts at: 5440 5440 Size: 6541 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6541 PfoI T'CCnGG_A Cuts at: 2172 3760 2172 Size: 1588 4953 PmeI GTTT'AAAC Cuts at: 3845 3845 Size: 6541 PmlI CAC'GTG Cuts at: 2921 3588 2921 Size: 667 5874 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4610 4642 6123 6155 4610 Size: 32 1481 32 4996 Fragments arranged by size: 4996 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3401 3401 Size: 6541 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6541 PsiI TTA'TAA Cuts at: 4111 4111 Size: 6541 PspOMI G'GGCC_C Cuts at: 3494 3494 Size: 6541 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3533 3565 3533 Size: 32 6509 PstI C_TGCA'G Cuts at: 830 2120 2498 2751 3893 830 Size: 1290 378 253 1142 3478 Fragments arranged by size: 3478 1290 1142 378 253 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6541 PvuII CAG'CTG Cuts at: 2499 2499 Size: 6541 SacI G_AGCT'C Cuts at: 2307 2307 Size: 6541 SalI G'TCGA_C Cuts at: 1936 3375 3883 1936 Size: 1439 508 4594 Fragments arranged by size: 4594 1439 508 SanDI GG'GwC_CC Cuts at: 3401 3401 Size: 6541 SapI GCTCTTCn'nnn_ Cuts at: 2447 3101 2447 Size: 654 5887 SbfI CC_TGCA'GG Cuts at: 3893 3893 Size: 6541 ScaI AGT'ACT Cuts at: 3551 4789 6501 3551 Size: 1238 1712 3591 Fragments arranged by size: 3591 1712 1238 SfcI C'TryA_G Cuts at: 826 1045 2116 2494 2747 3889 5024 5705 5896 Size: 219 1071 378 253 1142 1135 681 191 Cuts at: 5896 826 Size: 1471 Fragments arranged by size: 1471 1142 1135 1071 681 378 253 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6541 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6541 SmaI CCC'GGG Cuts at: 1872 3868 1872 Size: 1996 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2233 3191 4645 5546 5808 820 Size: 197 926 290 958 1454 901 262 1553 Fragments arranged by size: 1553 1454 958 926 901 290 262 197 SnaBI TAC'GTA Cuts at: 493 4332 493 Size: 3839 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6541 SphI G_CATG'C Cuts at: 1132 3899 1132 Size: 2767 3774 SspI AAT'ATT Cuts at: 5 52 4465 5 Size: 47 4413 2081 Fragments arranged by size: 4413 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6541 StyI C'CwwG_G Cuts at: 513 1065 2004 3713 3784 3980 513 Size: 552 939 1709 71 196 3074 Fragments arranged by size: 3074 1709 939 552 196 71 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3125 4568 4585 4738 4923 5368 325 Size: 1255 1545 1443 17 153 185 445 1498 Fragments arranged by size: 1545 1498 1443 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3549 4787 6499 Size: 274 80 33 51 1443 1572 1238 1712 Cuts at: 6499 96 Size: 138 Fragments arranged by size: 1712 1572 1443 1238 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6501 6533 6501 Size: 32 6509 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6541 XbaI T'CTAG_A Cuts at: 3877 3877 Size: 6541 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3003 3119 3151 3176 3003 Size: 116 32 25 6368 Fragments arranged by size: 6368 116 32 25 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6541 XmaI C'CCGG_G Cuts at: 1870 3866 1870 Size: 1996 4545 XmnI GAAnn'nnTTC Cuts at: 2162 2486 4670 2162 Size: 324 2184 4033 Fragments arranged by size: 4033 2184 324 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3830 4349 276 Size: 53 83 186 936 2296 519 2468 Fragments arranged by size: 2468 2296 936 519 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbeI BbvCI BfrBI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI RsrII SacII SexAI SfiI SfoI SrfI SwaI