(Circular) (Six-base) MAPSORT of: pFN21AB3889.seq Check: 8783 from: 1 to: 7129 pFN21AB3889 7129 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3943 3943 Size: 7129 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3104 4939 278 Size: 53 83 186 936 1568 1835 2468 Fragments arranged by size: 2468 1835 1568 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4472 1937 Size: 2535 4594 Acc65I G'GTAC_C Cuts at: 3999 4450 3999 Size: 451 6678 AclI AA'CG_TT Cuts at: 1563 5256 5629 1563 Size: 3693 373 3063 Fragments arranged by size: 3693 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2596 2660 3320 3646 3725 5192 6576 6844 Size: 964 64 660 326 79 1467 1384 268 Cuts at: 6844 1632 Size: 1917 Fragments arranged by size: 1917 1467 1384 964 660 326 268 79 64 AfeI AGC'GCT Cuts at: 3431 4272 3431 Size: 841 6288 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6932 AflIII A'CryG_T Cuts at: 1203 6028 7005 1203 Size: 4825 977 1327 Fragments arranged by size: 4825 1327 977 AgeI A'CCGG_T Cuts at: 2556 5960 2556 Size: 3404 3725 AhdI GACnn_n'nnGTC Cuts at: 2256 5858 2256 Size: 3602 3527 AleI CACnn'nnGTG Cuts at: 1166 3567 1166 Size: 2401 4728 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3752 1184 Size: 2568 4561 AlwNI CAG_nnn'CTG Cuts at: 2837 3544 3610 3739 4872 6444 2837 Size: 707 66 129 1133 1572 3522 Fragments arranged by size: 3522 1572 1133 707 129 66 ApaI G_GGCC'C Cuts at: 2876 3039 3526 2876 Size: 163 487 6479 Fragments arranged by size: 6479 487 163 ApaLI G'TGCA_C Cuts at: 2465 5186 6342 2465 Size: 2721 1156 3252 Fragments arranged by size: 3252 2721 1156 ApoI r'AATT_y Cuts at: 1493 4438 4664 4816 6925 1493 Size: 2945 226 152 2109 1697 Fragments arranged by size: 2945 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5683 160 Size: 872 4651 1606 Fragments arranged by size: 4651 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7129 AvaI C'yCGr_G Cuts at: 1870 1943 2164 3038 3885 4235 4454 1870 Size: 73 221 874 847 350 219 4545 Fragments arranged by size: 4545 874 847 350 221 219 73 BamHI G'GATC_C Cuts at: 4459 4826 4459 Size: 367 6762 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2135 3278 3632 3999 4450 Size: 325 217 627 348 1143 354 367 451 Cuts at: 4450 5905 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1143 627 451 367 354 348 325 217 BanII G_rGCy'C Cuts at: 1391 2876 3039 3526 4448 1391 Size: 1485 163 487 922 4072 Fragments arranged by size: 4072 1485 922 487 163 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3544 928 Size: 971 1645 4513 Fragments arranged by size: 4513 1645 971 BbvCI CC'TCA_GC Cuts at: 2225 3622 2225 Size: 1397 5732 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2683 2717 5320 5354 1903 Size: 34 746 34 2603 34 3678 Fragments arranged by size: 3678 2603 746 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5021 6231 5021 Size: 1210 5919 BclI T'GATC_A Cuts at: 1548 1548 Size: 7129 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2168 2261 2339 Size: 107 122 71 631 605 496 93 78 Cuts at: 2339 2348 5740 136 Size: 9 3392 1525 Fragments arranged by size: 3392 1525 631 605 496 122 107 93 78 71 9 BglII A'GATC_T Cuts at: 2655 7124 2655 Size: 4469 2660 BlpI GC'TnA_GC Cuts at: 2636 4316 4546 2636 Size: 1680 230 5219 Fragments arranged by size: 5219 1680 230 Bme1580I G_kGCm'C Cuts at: 1790 2469 2876 3039 3281 3526 3652 3690 5190 Size: 679 407 163 242 245 126 38 1500 Cuts at: 5190 6346 1790 Size: 1156 2573 Fragments arranged by size: 2573 1500 1156 679 407 245 242 163 126 38 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7129 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3879 4860 5818 Size: 416 53 83 399 24 2462 981 958 Cuts at: 5818 442 Size: 1753 Fragments arranged by size: 2462 1753 981 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2378 1056 Size: 1322 5807 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2986 3911 2986 Size: 925 6204 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 3232 3638 4313 5789 1239 Size: 968 1025 406 675 1476 2579 Fragments arranged by size: 2579 1476 1025 968 675 406 Bpu10I CC'TnA_GC Cuts at: 2225 3249 3622 2225 Size: 1024 373 5732 Fragments arranged by size: 5732 1024 373 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3656 4240 4295 5254 6119 6417 3656 Size: 584 55 959 865 298 4368 Fragments arranged by size: 4368 959 865 584 298 55 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2006 5792 882 Size: 617 507 3786 2219 Fragments arranged by size: 3786 2219 617 507 BsaAI yAC'GTr Cuts at: 493 1204 4164 4920 493 Size: 711 2960 756 2702 Fragments arranged by size: 2960 2702 756 711 BsaBI GATnn'nnATC Cuts at: 2660 7123 2660 Size: 4463 2666 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3101 4936 5318 5953 Size: 53 83 186 936 1568 1835 382 635 Cuts at: 5953 275 Size: 1451 Fragments arranged by size: 1835 1568 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2556 3024 3159 3257 4071 5562 5960 6234 6381 Size: 468 135 98 814 1491 398 274 147 Cuts at: 6381 6792 2556 Size: 411 2893 Fragments arranged by size: 2893 1491 814 468 411 398 274 147 135 98 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2348 2378 2738 2768 1178 Size: 30 165 30 945 30 360 30 5539 Fragments arranged by size: 5539 945 360 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2503 2759 3023 3944 3962 4253 1186 Size: 1317 256 264 921 18 291 4062 Fragments arranged by size: 4062 1317 921 291 264 256 18 BseYI C'CCAG_C Cuts at: 2757 2761 2862 3544 4012 4207 6332 6828 2757 Size: 4 101 682 468 195 2125 496 3058 Fragments arranged by size: 3058 2125 682 496 468 195 101 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2881 3319 3821 1163 Size: 573 1145 438 502 4471 Fragments arranged by size: 4471 1145 573 502 438 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2022 2647 4386 4579 5340 6368 Size: 275 186 21 625 1739 193 761 1028 Cuts at: 6368 1540 Size: 2301 Fragments arranged by size: 2301 1739 1028 761 625 275 193 186 21 BsiHKAI G_wGCw'C Cuts at: 2469 4218 5190 5275 6346 2469 Size: 1749 972 85 1071 3252 Fragments arranged by size: 3252 1749 1071 972 85 BsmI GAATG_Cn' Cuts at: 4640 4733 4640 Size: 93 7036 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2469 2876 3039 3281 3526 3652 3690 Size: 399 679 407 163 242 245 126 38 Cuts at: 3690 4218 4448 5190 5275 6346 1391 Size: 528 230 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 679 528 407 399 245 242 230 163 126 85 38 BspEI T'CCGG_A Cuts at: 3159 4071 6792 3159 Size: 912 2721 3496 Fragments arranged by size: 3496 2721 912 BspHI T'CATG_A Cuts at: 5018 6748 5018 Size: 1730 5399 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3943 3990 4011 4484 844 Size: 3099 47 21 473 3489 Fragments arranged by size: 3489 3099 473 47 21 BsrBI CCG'CTC Cuts at: 1394 1823 4300 5016 1394 Size: 429 2477 716 3507 Fragments arranged by size: 3507 2477 716 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2264 5624 5798 65 Size: 1183 1016 3360 174 1396 Fragments arranged by size: 3360 1396 1183 1016 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2556 2915 3564 3846 4381 5773 5960 Size: 110 746 359 649 282 535 1392 187 Cuts at: 5960 1700 Size: 2869 Fragments arranged by size: 2869 1392 746 649 535 359 282 187 110 BsrGI T'GTAC_A Cuts at: 96 2850 96 Size: 2754 4375 BssHII G'CGCG_C Cuts at: 1925 4419 1925 Size: 2494 4635 BssSI C'ACGA_G Cuts at: 2692 3761 3815 4190 5189 6201 2692 Size: 1069 54 375 999 1012 3620 Fragments arranged by size: 3620 1069 1012 999 375 54 BstAPI GCAn_nnn'nTGC Cuts at: 794 3285 794 Size: 2491 4638 BstBI TT'CG_AA Cuts at: 5933 5933 Size: 7129 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7129 BstXI CCAn_nnnn'nTGG Cuts at: 3569 4030 3569 Size: 461 6668 BstYI r'GATC_y Cuts at: 1972 2655 3114 4459 4826 5211 5228 6680 6766 Size: 683 459 1345 367 385 17 1452 86 Cuts at: 6766 6778 7124 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1345 683 459 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4083 4083 Size: 7129 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 2337 2346 2520 3121 3597 Size: 552 939 12 321 9 174 601 476 Cuts at: 3597 3615 3724 513 Size: 18 109 3918 Fragments arranged by size: 3918 939 601 552 476 321 174 109 18 12 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6390 Fragments arranged by size: 6390 683 56 BtsI GCAGTG_nn' Cuts at: 2211 2546 2849 2893 3838 4183 4645 5439 5459 Size: 335 303 44 945 345 462 794 20 Cuts at: 5459 2211 Size: 3881 Fragments arranged by size: 3881 945 794 462 345 335 303 44 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7129 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7094 DraI TTT'AAA Cuts at: 4433 4433 Size: 7129 DraIII CAC_nnn'GTG Cuts at: 2473 3274 4048 2473 Size: 801 774 5554 Fragments arranged by size: 5554 801 774 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3887 6136 809 Size: 507 2571 2249 1802 Fragments arranged by size: 2571 2249 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 2110 2340 Size: 54 1425 50 275 18 189 91 230 Cuts at: 2340 2517 2623 2644 3124 3229 3486 3510 3612 Size: 177 106 21 480 105 257 24 102 Cuts at: 3612 4020 4383 4576 5465 7094 8 Size: 408 363 193 889 1629 43 Fragments arranged by size: 1629 1425 889 480 408 363 275 257 230 193 189 177 106 105 102 91 54 50 43 24 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2019 2644 4383 4576 1537 Size: 275 207 625 1739 193 4090 Fragments arranged by size: 4090 1739 625 275 207 193 EarI CTCTTCn'nnn_ Cuts at: 1353 2126 2513 3360 5059 1353 Size: 773 387 847 1699 3423 Fragments arranged by size: 3423 1699 847 773 387 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2100 3932 5716 6090 6236 387 Size: 1064 138 511 1832 1784 374 146 1280 Fragments arranged by size: 1832 1784 1280 1064 511 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2596 2660 3232 3320 3638 3646 Size: 393 575 389 64 572 88 318 8 Cuts at: 3646 3725 4313 5192 5789 6576 6844 1239 Size: 79 588 879 597 787 268 1524 Fragments arranged by size: 1524 879 787 597 588 575 572 393 389 318 268 88 79 64 8 EcoNI CCTnn'n_nnAGG Cuts at: 2315 3410 3899 2315 Size: 1095 489 5545 Fragments arranged by size: 5545 1095 489 EcoO109I rG'GnC_Cy Cuts at: 2671 2873 3523 4078 4141 4231 4279 4323 2671 Size: 202 650 555 63 90 48 44 5477 Fragments arranged by size: 5477 650 555 202 90 63 48 44 EcoRI G'AATT_C Cuts at: 4438 4816 4438 Size: 378 6751 EcoRV GAT'ATC Cuts at: 3136 3136 Size: 7129 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3325 3730 3325 Size: 405 6724 FspI TGC'GCA Cuts at: 5635 5635 Size: 7129 HaeII r_GCGC'y Cuts at: 1678 2595 2619 3433 4013 4274 6276 1678 Size: 917 24 814 580 261 2002 2531 Fragments arranged by size: 2531 2002 917 814 580 261 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2242 2274 2348 2380 2738 Size: 32 135 32 670 32 74 32 358 Cuts at: 2738 2770 5770 5802 5844 5876 1373 Size: 32 3000 32 42 32 2626 Fragments arranged by size: 3000 2626 670 358 135 74 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2146 4473 4719 669 Size: 988 281 208 2327 246 3079 Fragments arranged by size: 3079 2327 988 281 246 208 HindIII A'AGCT_T Cuts at: 748 748 Size: 7129 HpaI GTT'AAC Cuts at: 4719 4719 Size: 7129 KpnI G_GTAC'C Cuts at: 4003 4454 4003 Size: 451 6678 MfeI C'AATT_G Cuts at: 4728 4728 Size: 7129 MluI A'CGCG_T Cuts at: 7005 7005 Size: 7129 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4141 6243 6427 1305 Size: 2836 2102 184 2007 Fragments arranged by size: 2836 2102 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2112 2342 3231 3488 3614 Size: 54 1425 343 280 230 889 257 126 Cuts at: 3614 4022 10 Size: 408 3117 Fragments arranged by size: 3117 1425 889 408 343 280 257 230 126 54 MslI CAynn'nnrTG Cuts at: 518 1166 2537 3567 5087 5446 5605 518 Size: 648 1371 1030 1520 359 159 2042 Fragments arranged by size: 2042 1520 1371 1030 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2018 2098 2761 2798 3183 3541 3626 3666 Size: 412 80 663 37 385 358 85 40 Cuts at: 3666 3736 3747 4312 4545 5222 6370 6615 1606 Size: 70 11 565 233 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 663 565 412 385 358 245 233 85 80 70 40 37 11 NaeI GCC'GGC Cuts at: 2917 3848 4383 2917 Size: 931 535 5663 Fragments arranged by size: 5663 931 535 NcoI C'CATG_G Cuts at: 513 1065 2004 2346 3597 513 Size: 552 939 342 1251 4045 Fragments arranged by size: 4045 1251 939 552 342 NdeI CA'TA_TG Cuts at: 387 387 Size: 7129 NgoMIV G'CCGG_C Cuts at: 2915 3846 4381 2915 Size: 931 535 5663 Fragments arranged by size: 5663 931 535 NheI G'CTAG_C Cuts at: 1052 2374 1052 Size: 1322 5807 NotI GC'GGCC_GC Cuts at: 4576 4576 Size: 7129 NspI r_CATG'y Cuts at: 1132 1229 4487 6032 1132 Size: 97 3258 1545 2229 Fragments arranged by size: 3258 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2876 4401 1348 Size: 1528 1525 4076 Fragments arranged by size: 4076 1528 1525 PciI A'CATG_T Cuts at: 6028 6028 Size: 7129 PflMI CCAn_nnn'nTGG Cuts at: 1108 3519 3544 3610 4401 1108 Size: 2411 25 66 791 3836 Fragments arranged by size: 3836 2411 791 66 25 PfoI T'CCnGG_A Cuts at: 3654 3654 Size: 7129 PmeI GTTT'AAAC Cuts at: 4433 4433 Size: 7129 PmlI CAC'GTG Cuts at: 4164 4164 Size: 7129 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5198 5230 6711 6743 4160 Size: 32 1006 32 1481 32 4546 Fragments arranged by size: 4546 1481 1006 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2671 4078 4323 2671 Size: 1407 245 5477 Fragments arranged by size: 5477 1407 245 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7129 PsiI TTA'TAA Cuts at: 4699 4699 Size: 7129 PspOMI G'GGCC_C Cuts at: 2872 3035 3522 2872 Size: 163 487 6479 Fragments arranged by size: 6479 487 163 PstI C_TGCA'G Cuts at: 830 2553 2985 3099 3414 3845 4481 830 Size: 1723 432 114 315 431 636 3478 Fragments arranged by size: 3478 1723 636 432 431 315 114 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7129 PvuII CAG'CTG Cuts at: 2098 2761 2798 3183 3541 3626 3666 3736 2098 Size: 663 37 385 358 85 40 70 5491 Fragments arranged by size: 5491 663 385 358 85 70 40 37 SacII CC_GC'GG Cuts at: 2019 2019 Size: 7129 SalI G'TCGA_C Cuts at: 1936 4471 1936 Size: 2535 4594 SanDI GG'GwC_CC Cuts at: 4078 4078 Size: 7129 SapI GCTCTTCn'nnn_ Cuts at: 2513 2513 Size: 7129 SbfI CC_TGCA'GG Cuts at: 3414 4481 3414 Size: 1067 6062 ScaI AGT'ACT Cuts at: 5377 7089 5377 Size: 1712 5417 SexAI A'CCwGG_T Cuts at: 3603 3603 Size: 7129 SfcI C'TryA_G Cuts at: 826 1045 2549 2587 2981 3001 3095 3410 3841 Size: 219 1504 38 394 20 94 315 431 Cuts at: 3841 4477 5612 6293 6484 826 Size: 636 1135 681 191 1471 Fragments arranged by size: 1504 1471 1135 681 636 431 394 315 219 191 94 38 20 SfiI GGCCn_nnn'nGGCC Cuts at: 2168 2339 2348 2168 Size: 171 9 6949 Fragments arranged by size: 6949 171 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7129 SgrAI Cr'CCGG_yG Cuts at: 1700 2915 3564 1700 Size: 1215 649 5265 Fragments arranged by size: 5265 1215 649 SmaI CCC'GGG Cuts at: 1872 4456 1872 Size: 2584 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3671 4219 4274 5233 6134 6396 Size: 197 926 1728 548 55 959 901 262 Cuts at: 6396 820 Size: 1553 Fragments arranged by size: 1728 1553 959 926 901 548 262 197 55 SnaBI TAC'GTA Cuts at: 493 4920 493 Size: 4427 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7129 SphI G_CATG'C Cuts at: 1132 4487 1132 Size: 3355 3774 SspI AAT'ATT Cuts at: 5 52 5053 5 Size: 47 5001 2081 Fragments arranged by size: 5001 2081 47 StuI AGG'CCT Cuts at: 1517 2781 4088 1517 Size: 1264 1307 4558 Fragments arranged by size: 4558 1307 1264 StyI C'CwwG_G Cuts at: 513 1065 2004 2346 2806 3189 3597 4568 513 Size: 552 939 342 460 383 408 971 3074 Fragments arranged by size: 3074 971 939 552 460 408 383 342 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2753 3536 3926 4199 5156 5173 5326 Size: 1255 1173 783 390 273 957 17 153 Cuts at: 5326 5511 5956 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1173 957 783 445 390 273 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2784 2850 5375 Size: 274 80 33 51 1443 807 66 2525 Cuts at: 5375 7087 96 Size: 1712 138 Fragments arranged by size: 2525 1712 1443 807 274 138 80 66 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2427 2459 4340 4372 7089 7121 2427 Size: 32 1881 32 2717 32 2435 Fragments arranged by size: 2717 2435 1881 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3824 1316 Size: 2508 4621 XbaI T'CTAG_A Cuts at: 4465 4465 Size: 7129 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2050 3866 2050 Size: 1816 5313 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7129 XmaI C'CCGG_G Cuts at: 1870 4454 1870 Size: 2584 4545 XmnI GAAnn'nnTTC Cuts at: 2990 5258 2990 Size: 2268 4861 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3102 4937 276 Size: 53 83 186 936 1568 1835 2468 Fragments arranged by size: 2468 1835 1568 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SfoI SrfI SwaI