Restriction map for
pFN21AB3870
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbrPI BbvCI BcgI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspMI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HincII HindII
HpaI KpnI KspI MaeI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 265 GTCTAC GT^[AC][GT]AC
AccII 511 CGCG CG^CG
AflIII 653 ACATGT A^C[GA][CT]GT
AlwNI 811 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 933 CCTGAGG CC^T[ACGT]AGG
AspI 723 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 61 CCCGGG C^[CT]CG[GA]G
BbsI 1032 GTCTTC GAAGAC(2/6)
BciVI 3 GTATCC GTATCC(6/5)
BglI 274 GCCGGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 353 AGATCT A^GATCT
BpuAI 1032 GTCTTC GAAGAC(2/6)
BsaAI 451 TACGTG [CT]AC^GT[GA]
BseRI 838 GAGGAG GAGGAG(10/8)
BsmBI 376 GAGACG CGTCTC(1/5)
BsoBI 61 CCCGGG C^[CT]CG[GA]G
BspLU11I 653 ACATGT A^CATGT
BsrBI 382 GAGCGG CCGCTC(-3/-3)
BssSI 1045 CACGAG CACGAG(-5/-1)
BstEII 171 GGTGACC G^GT[ACGT]ACC
BstPI 171 GGTGACC G^GT[ACGT]ACC
BstUI 511 CGCG CG^CG
Bsu36I 933 CCTGAGG CC^T[ACGT]AGG
Eco81I 933 CCTGAGG CC^T[ACGT]AGG
EcoNI 831 CCTCAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 171 GGTGACC G^GT[ACGT]ACC
HindIII 422 AAGCTT A^AGCTT
MseI 308 TTAA T^TAA
MslI 489 CACCCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 511 CGCG CG^CG
NcoI 575 CCATGG C^CATGG
NspI 653 ACATGT [GA]CATG^[CT]
PflFI 723 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1013 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 519 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 570 GAGCTC GAGCT^C
ScaI 76 AGTACT AGT^ACT
SexAI 724 ACCTGGT A^CC[AT]GGT
SmaI 61 CCCGGG CCC^GGG
SmlI 786 CTCAAG C^T[CT][GA]AG
StuI 590 AGGCCT AGG^CCT
TfiI 10 GAATC G^A[AT]TC
Tru9I 308 TTAA T^TAA
Tth111I 723 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 61 CCCGGG C^CCGGG
XmaI 61 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 715 GGCGCC G[GA]^CG[CT]C
848 GGCGCC
AlwI 828 GATCC GGATC(4/5)
1097 GATCC
AspHI 570 GAGCTC G[AT]GC[AT]^C
958 GTGCTC
BalI 542 TGGCCA TGG^CCA
917 TGGCCA
BbeI 715 GGCGCC GGCGC^C
848 GGCGCC
BsaHI 715 GGCGCC G[GA]^CG[CT]C
848 GGCGCC
BsaI 502 GAGACC GGTCTC(1/5)
144 GGTCTC
BsgI 900 CTGCAC GTGCAG(16/14)
679 GTGCAG
BsiHKAI 570 GAGCTC G[AT]GC[AT]^C
958 GTGCTC
BsrFI 274 GCCGGC [GA]^CCGG[CT]
845 GCCGGC
Cfr10I 274 GCCGGC [GA]^CCGG[CT]
845 GCCGGC
DsaI 575 CCATGG C^C[GA][CT]GG
599 CCGTGG
EaeI 542 TGGCCA [CT]^GGCC[GA]
917 TGGCCA
EarI 247 CTCTTC CTCTTC(1/4)
499 GAAGAG
EcoT14I 221 CCTTGG C^C[AT][AT]GG
575 CCATGG
Hin1I 715 GGCGCC G[GA]^CG[CT]C
848 GGCGCC
KasI 715 GGCGCC G^GCGCC
848 GGCGCC
Ksp632I 247 CTCTTC CTCTTC(1/4)
499 GAAGAG
MaeIII 172 GTGAC ^GT[ACGT]AC
561 GTCAC
MluNI 542 TGGCCA TGG^CCA
917 TGGCCA
MscI 542 TGGCCA TGG^CCA
917 TGGCCA
NaeI 274 GCCGGC GCC^GGC
845 GCCGGC
NarI 715 GGCGCC GG^CGCC
848 GGCGCC
NgoMIV 274 GCCGGC G^CCGGC
845 GCCGGC
PleI 90 GAGTC GAGTC(4/5)
947 GAGTC
PstI 439 CTGCAG CTGCA^G
874 CTGCAG
SfaNI 239 GCATC GCATC(5/9)
773 GCATC
SfoI 715 GGCGCC GGC^GCC
848 GGCGCC
StyI 221 CCTTGG C^C[AT][AT]GG
575 CCATGG
TaqI 251 TCGA T^CGA
410 TCGA
Tsp45I 172 GTGAC ^GT[GC]AC
561 GTCAC
TthHB8I 251 TCGA T^CGA
410 TCGA
XcmI 485 CCAACACCCCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
575 CCATGGAGATCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI TseI TspRI Van91I XhoII