Restriction map for
pFN21AB3870
(Circular) (Six-base) MAPSORT of: pFN21AB3870.seq Check: 5400 from: 1 to: 5806
pFN21AB3870 5806 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3616 278
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2271 3149 1937
Size: 334 878 4594
Fragments arranged by size:
4594 878 334
Acc65I G'GTAC_C
Cuts at: 3127 3127
Size: 5806
AclI AA'CG_TT
Cuts at: 1563 3933 4306 1563
Size: 2370 373 3063
Fragments arranged by size:
3063 2370 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2310 2843 2891 3869 5253 5521 1632
Size: 678 533 48 978 1384 268 1917
Fragments arranged by size:
1917 1384 978 678 533 268 48
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5609
AflIII A'CryG_T
Cuts at: 1203 2658 4705 5682 1203
Size: 1455 2047 977 1327
Fragments arranged by size:
2047 1455 1327 977
AgeI A'CCGG_T
Cuts at: 4637 4637
Size: 5806
AhdI GACnn_n'nnGTC
Cuts at: 4535 4535
Size: 5806
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5806
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5806
AlwNI CAG_nnn'CTG
Cuts at: 2821 3549 5121 2821
Size: 728 1572 3506
Fragments arranged by size:
3506 1572 728
ApaLI G'TGCA_C
Cuts at: 3863 5019 3863
Size: 1156 4650
ApoI r'AATT_y
Cuts at: 1493 3115 3341 3493 5602 1493
Size: 1622 226 152 2109 1697
Fragments arranged by size:
2109 1697 1622 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4360 160
Size: 872 3328 1606
Fragments arranged by size:
3328 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 3131 1870
Size: 73 123 1065 4545
Fragments arranged by size:
4545 1065 123 73
BamHI G'GATC_C
Cuts at: 3136 3503 3136
Size: 367 5439
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2720 2853 2934 3127 4582
Size: 325 217 627 933 133 81 193 1455
Cuts at: 4582 618
Size: 1842
Fragments arranged by size:
1842 1455 933 627 325 217 193 133
81
BanII G_rGCy'C
Cuts at: 1391 2030 2063 2579 3125 1391
Size: 639 33 516 546 4072
Fragments arranged by size:
4072 639 546 516 33
BbeI G_GCGC'C
Cuts at: 2724 2857 2724
Size: 133 5673
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3030 928
Size: 971 1131 3704
Fragments arranged by size:
3704 1131 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3997 4031 1903
Size: 34 2060 34 3678
Fragments arranged by size:
3678 2060 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2019 3698 4908 2019
Size: 1679 1210 2917
Fragments arranged by size:
2917 1679 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5806
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2285 4417 136
Size: 107 122 71 631 605 613 2132 1525
Fragments arranged by size:
2132 1525 631 613 605 122 107 71
BglII A'GATC_T
Cuts at: 2358 5801 2358
Size: 3443 2363
BlpI GC'TnA_GC
Cuts at: 3223 3223
Size: 5806
Bme1580I G_kGCm'C
Cuts at: 1790 2564 3867 5023 1790
Size: 774 1303 1156 2573
Fragments arranged by size:
2573 1303 1156 774
BmgBI CAC'GTC
Cuts at: 1318 2530 2565 1318
Size: 1212 35 4559
Fragments arranged by size:
4559 1212 35
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2309 2566 3537
Size: 416 53 83 399 24 892 257 971
Cuts at: 3537 4495 442
Size: 958 1753
Fragments arranged by size:
1753 971 958 892 416 399 257 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5806
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2617 2617
Size: 5806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2456 2471 2593 4466 1239
Size: 857 360 15 122 1873 2579
Fragments arranged by size:
2579 1873 857 360 122 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2776 3931 4796 5094 2776
Size: 1155 865 298 3488
Fragments arranged by size:
3488 1155 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2155 2501 4469 882
Size: 617 656 346 1968 2219
Fragments arranged by size:
2219 1968 656 617 346
BsaAI yAC'GTr
Cuts at: 493 1204 2458 3597 493
Size: 711 1254 1139 2702
Fragments arranged by size:
2702 1254 1139 711
BsaBI GATnn'nnATC
Cuts at: 5800 5800
Size: 5806
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2721 2854 3613 3995
Size: 53 83 186 936 1188 133 759 382
Cuts at: 3995 4630 275
Size: 635 1451
Fragments arranged by size:
1451 1188 936 759 635 382 186 133
83 53
BsaWI w'CCGG_w
Cuts at: 4239 4637 4911 5058 5469 4239
Size: 398 274 147 411 4576
Fragments arranged by size:
4576 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5581
Fragments arranged by size:
5581 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2858 1186
Size: 1672 4134
BseYI C'CCAG_C
Cuts at: 2916 5009 5505 2916
Size: 2093 496 3217
Fragments arranged by size:
3217 2093 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2705 2890 1163
Size: 573 969 185 4079
Fragments arranged by size:
4079 969 573 185
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3256 4017 5045 1540
Size: 275 186 1255 761 1028 2301
Fragments arranged by size:
2301 1255 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2579 2967 3867 3952 5023 2579
Size: 388 900 85 1071 3362
Fragments arranged by size:
3362 1071 900 388 85
BsmI GAATG_Cn'
Cuts at: 3317 3410 3317
Size: 93 5713
BsmBI CGTCTCn'nnnn_
Cuts at: 2375 2375
Size: 5806
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2063 2564 2579 2967 3125 3867
Size: 399 240 33 501 15 388 158 742
Cuts at: 3867 3952 5023 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 501 399 388 240 158
85 33 15
BspEI T'CCGG_A
Cuts at: 5469 5469
Size: 5806
BspHI T'CATG_A
Cuts at: 3695 5425 3695
Size: 1730 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3161 844
Size: 2317 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2389 3693 1394
Size: 429 566 1304 3507
Fragments arranged by size:
3507 1304 566 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4301 4475 65
Size: 1183 3053 174 1396
Fragments arranged by size:
3053 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2279 2850 4450 4637 1700
Size: 110 469 571 1600 187 2869
Fragments arranged by size:
2869 1600 571 469 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5806
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5806
BssSI C'ACGA_G
Cuts at: 3050 3866 4878 3050
Size: 816 1012 3978
Fragments arranged by size:
3978 1012 816
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5806
BstBI TT'CG_AA
Cuts at: 4610 4610
Size: 5806
BstEII G'GTnAC_C
Cuts at: 1184 2176 1184
Size: 992 4814
BstYI r'GATC_y
Cuts at: 1972 2358 2832 3101 3136 3503 3888 3905 5357
Size: 386 474 269 35 367 385 17 1452
Cuts at: 5357 5443 5455 5801 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 474 386 385 367 346 269
86 35 17 12
Bsu36I CC'TnA_GG
Cuts at: 2939 2939
Size: 5806
BtgI C'CryG_G
Cuts at: 513 1065 2580 2604 513
Size: 552 1515 24 3715
Fragments arranged by size:
3715 1515 552 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2872 487
Size: 683 56 1646 3421
Fragments arranged by size:
3421 1646 683 56
BtsI GCAGTG_nn'
Cuts at: 3322 4116 4136 3322
Size: 794 20 4992
Fragments arranged by size:
4992 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5806
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5771
DraI TTT'AAA
Cuts at: 3110 3110
Size: 5806
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4813 809
Size: 507 3497 1802
Fragments arranged by size:
3497 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2547 2922 3253
Size: 54 1425 50 275 18 717 375 331
Cuts at: 3253 4142 5771 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 717 375 331 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3253 1537
Size: 275 1441 4090
Fragments arranged by size:
4090 1441 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2258 2499 3736 1353
Size: 905 241 1237 3423
Fragments arranged by size:
3423 1237 905 241
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2385 2393 4393 4767 4913 387
Size: 1064 138 796 8 2000 374 146 1280
Fragments arranged by size:
2000 1280 1064 796 374 146 138 8
EcoICRI GAG'CTC
Cuts at: 2577 2577
Size: 5806
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2310 2456 2471 2593 2843 2891
Size: 393 464 214 146 15 122 250 48
Cuts at: 2891 3869 4466 5253 5521 1239
Size: 978 597 787 268 1524
Fragments arranged by size:
1524 978 787 597 464 393 268 250
214 146 122 48 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2840 2840
Size: 5806
EcoO109I rG'GnC_Cy
Cuts at: 2392 2973 3019 2392
Size: 581 46 5179
Fragments arranged by size:
5179 581 46
EcoRI G'AATT_C
Cuts at: 3115 3493 3115
Size: 378 5428
FspI TGC'GCA
Cuts at: 4312 4312
Size: 5806
HaeII r_GCGC'y
Cuts at: 1678 2724 2857 2899 4953 1678
Size: 1046 133 42 2054 2531
Fragments arranged by size:
2531 2054 1046 133 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2178 2210 3086 3118 4447
Size: 32 135 32 606 32 876 32 1329
Cuts at: 4447 4479 4521 4553 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1329 876 606 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3150 3396 669
Size: 988 281 1212 246 3079
Fragments arranged by size:
3079 1212 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2427 748
Size: 1679 4127
HpaI GTT'AAC
Cuts at: 3396 3396
Size: 5806
KasI G'GCGC_C
Cuts at: 2720 2853 2720
Size: 133 5673
KpnI G_GTAC'C
Cuts at: 3131 3131
Size: 5806
MfeI C'AATT_G
Cuts at: 3405 3405
Size: 5806
MluI A'CGCG_T
Cuts at: 5682 5682
Size: 5806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4920 5104 1305
Size: 3615 184 2007
Fragments arranged by size:
3615 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2549 2924 10
Size: 54 1425 343 717 375 2892
Fragments arranged by size:
2892 1425 717 375 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2498 3764 4123 4282 518
Size: 648 1332 1266 359 159 2042
Fragments arranged by size:
2042 1332 1266 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2449 2689 2920 3222 3899 5047 5292 1606
Size: 843 240 231 302 677 1148 245 2120
Fragments arranged by size:
2120 1148 843 677 302 245 240 231
NaeI GCC'GGC
Cuts at: 2281 2852 2281
Size: 571 5235
NarI GG'CG_CC
Cuts at: 2721 2854 2721
Size: 133 5673
NcoI C'CATG_G
Cuts at: 513 1065 2580 513
Size: 552 1515 3739
Fragments arranged by size:
3739 1515 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5806
NgoMIV G'CCGG_C
Cuts at: 2279 2850 2279
Size: 571 5235
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5806
NotI GC'GGCC_GC
Cuts at: 3253 3253
Size: 5806
NspI r_CATG'y
Cuts at: 1132 1229 2662 3164 4709 1132
Size: 97 1433 502 1545 2229
Fragments arranged by size:
2229 1545 1433 502 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5806
PciI A'CATG_T
Cuts at: 2658 4705 2658
Size: 2047 3759
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2545 2556 2730 2931 2995 1108
Size: 1437 11 174 201 64 3919
Fragments arranged by size:
3919 1437 201 174 64 11
PfoI T'CCnGG_A
Cuts at: 2399 2399
Size: 5806
PmeI GTTT'AAAC
Cuts at: 3110 3110
Size: 5806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 5388 5420 3875
Size: 32 1481 32 4261
Fragments arranged by size:
4261 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3019 3019
Size: 5806
PshAI GACnn'nnGTC
Cuts at: 1613 2528 1613
Size: 915 4891
PsiI TTA'TAA
Cuts at: 3376 3376
Size: 5806
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2645 2677 2645
Size: 32 5774
PstI C_TGCA'G
Cuts at: 830 2448 2883 3158 830
Size: 1618 435 275 3478
Fragments arranged by size:
3478 1618 435 275
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5806
PvuII CAG'CTG
Cuts at: 2449 2689 2920 2449
Size: 240 231 5335
Fragments arranged by size:
5335 240 231
SacI G_AGCT'C
Cuts at: 2579 2579
Size: 5806
SalI G'TCGA_C
Cuts at: 1936 3148 1936
Size: 1212 4594
SbfI CC_TGCA'GG
Cuts at: 3158 3158
Size: 5806
ScaI AGT'ACT
Cuts at: 2083 4054 5766 2083
Size: 1971 1712 2123
Fragments arranged by size:
2123 1971 1712
SexAI A'CCwGG_T
Cuts at: 2729 2729
Size: 5806
SfcI C'TryA_G
Cuts at: 826 1045 2077 2444 2879 3082 3097 3154 4289
Size: 219 1032 367 435 203 15 57 1135
Cuts at: 4289 4970 5161 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1032 681 435 367 219 203
191 57 15
SfoI GGC'GCC
Cuts at: 2722 2855 2722
Size: 133 5673
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5806
SmaI CCC'GGG
Cuts at: 1872 2068 3133 1872
Size: 196 1065 4545
Fragments arranged by size:
4545 1065 196
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2791 3910 4811 5073 820
Size: 197 926 848 1119 901 262 1553
Fragments arranged by size:
1553 1119 926 901 848 262 197
SnaBI TAC'GTA
Cuts at: 493 3597 493
Size: 3104 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5806
SphI G_CATG'C
Cuts at: 1132 3164 1132
Size: 2032 3774
SspI AAT'ATT
Cuts at: 5 52 3730 5
Size: 47 3678 2081
Fragments arranged by size:
3678 2081 47
StuI AGG'CCT
Cuts at: 1517 2597 1517
Size: 1080 4726
StyI C'CwwG_G
Cuts at: 513 1065 2226 2580 3245 513
Size: 552 1161 354 665 3074
Fragments arranged by size:
3074 1161 665 552 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2164 3833 3850 4003 4188 4633 325
Size: 1255 584 1669 17 153 185 445 1498
Fragments arranged by size:
1669 1498 1255 584 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2081 2553 4052
Size: 274 80 33 51 1443 104 472 1499
Cuts at: 4052 5764 96
Size: 1712 138
Fragments arranged by size:
1712 1499 1443 472 274 138 104 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2160 2192 5766 5798 2160
Size: 32 3574 32 2168
Fragments arranged by size:
3574 2168 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2731 1316
Size: 1415 4391
XbaI T'CTAG_A
Cuts at: 3142 3142
Size: 5806
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2497 2587 2497
Size: 90 5716
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5806
XmaI C'CCGG_G
Cuts at: 1870 2066 3131 1870
Size: 196 1065 4545
Fragments arranged by size:
4545 1065 196
XmnI GAAnn'nnTTC
Cuts at: 3935 3935
Size: 5806
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3614 276
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I DraIII EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI
RsrII SacII SanDI SapI SfiI SrfI SwaI