Restriction map for
pFN21AB3864
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AvrII BbrPI
BcgI BclI BfaI BfrI BlnI
BsaBI BseAI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspLU11I Bst1107I
BstBI BstZ17I ClaI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV FbaI FseI HindIII HpaI
KspI MaeI MamI MfeI MluI
MroI MseI MunI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SpeI SphI Sse8387I SspI
SwaI Tru9I Tsp509I Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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Acc65I 1805 GGTACC G^GTACC
Aor51HI 2630 AGCGCT AGC^GCT
Asp718I 1805 GGTACC G^GTACC
AviII 2459 TGCGCA TGC^GCA
BamHI 3041 GGATCC G^GATCC
BciVI 3101 GTATCC GTATCC(6/5)
BglII 2204 AGATCT A^GATCT
BlpI 2005 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2005 GCTCAGC GC^T[ACGT]AGC
BsaAI 983 TACGTA [CT]AC^GT[GA]
BsaWI 2794 TCCGGT [AT]^CCGG[AT]
Bsp1407I 3074 TGTACA T^GTACA
BspHI 1482 TCATGA T^CATGA
BsrGI 3074 TGTACA T^GTACA
BssHII 259 GCGCGC G^CGCGC
BstEII 1219 GGTCACC G^GT[ACGT]ACC
BstPI 1219 GGTCACC G^GT[ACGT]ACC
CelII 2005 GCTCAGC GC^T[ACGT]AGC
Eco47III 2630 AGCGCT AGC^GCT
EcoNI 42 CCTACCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1219 GGTCACC G^GT[ACGT]ACC
EcoT22I 3193 ATGCAT ATGCA^T
FspI 2459 TGCGCA TGC^GCA
HincII 2907 GTTGAC GT[CT]^[GA]AC
HindII 2907 GTTGAC GT[CT]^[GA]AC
KpnI 1805 GGTACC GGTAC^C
NsiI 3193 ATGCAT ATGCA^T
PshAI 1653 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1482 TCATGA T^CATGA
SnaBI 983 TACGTA TAC^GTA
SspBI 3074 TGTACA T^GTACA
StuI 528 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 22 enzymes) #
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AocI 470 CCTCAGG CC^T[ACGT]AGG
2302 CCTGAGG
BbeI 31 GGCGCC GGCGC^C
1799 GGCGCC
BsaI 2242 GAGACC GGTCTC(1/5)
2119 GGTCTC
BsgI 1840 CTGCAC GTGCAG(16/14)
3412 CTGCAC
BsrDI 1452 CATTGC GCAATG(2/0)
3307 CATTGC
Bsu36I 470 CCTCAGG CC^T[ACGT]AGG
2302 CCTGAGG
CpoI 1382 CGGTCCG CG^G[AT]CCG
2879 CGGTCCG
DraIII 928 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
1673 CACGGGGTG
Eco81I 470 CCTCAGG CC^T[ACGT]AGG
2302 CCTGAGG
KasI 31 GGCGCC G^GCGCC
1799 GGCGCC
NaeI 740 GCCGGC GCC^GGC
3138 GCCGGC
NarI 31 GGCGCC GG^CGCC
1799 GGCGCC
NcoI 1104 CCATGG C^CATGG
1130 CCATGG
NgoMIV 740 GCCGGC G^CCGGC
3138 GCCGGC
NspI 1202 ACATGC [GA]CATG^[CT]
3416 ACATGC
PvuII 3106 CAGCTG CAG^CTG
3214 CAGCTG
RsrII 1382 CGGTCCG CG^G[AT]CCG
2879 CGGTCCG
SacI 1056 GAGCTC GAGCT^C
2003 GAGCTC
SapI 1866 GCTCTTC GCTCTTC(1/4)
3324 GCTCTTC
SfoI 31 GGCGCC GGC^GCC
1799 GGCGCC
SmlI 1366 CTTGAG C^T[CT][GA]AG
2785 CTCAAG
TfiI 439 GAATC G^A[AT]TC
1083 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI