(Circular) (Six-base) MAPSORT of: pFN21AB3864.seq Check: 4408 from: 1 to: 8131 pFN21AB3864 8131 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2824 5219 2824 Size: 2395 5736 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5941 278 Size: 53 83 186 936 4405 2468 Fragments arranged by size: 4405 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5474 1937 Size: 3537 4594 Acc65I G'GTAC_C Cuts at: 3810 5452 3810 Size: 1642 6489 AclI AA'CG_TT Cuts at: 1563 6258 6631 1563 Size: 4695 373 3063 Fragments arranged by size: 4695 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3023 3263 3285 3765 6194 7578 7846 1632 Size: 1391 240 22 480 2429 1384 268 1917 Fragments arranged by size: 2429 1917 1391 1384 480 268 240 22 AfeI AGC'GCT Cuts at: 4637 4637 Size: 8131 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7934 AflIII A'CryG_T Cuts at: 1203 7030 8007 1203 Size: 5827 977 1327 Fragments arranged by size: 5827 1327 977 AgeI A'CCGG_T Cuts at: 6962 6962 Size: 8131 AhdI GACnn_n'nnGTC Cuts at: 6860 6860 Size: 8131 AleI CACnn'nnGTG Cuts at: 1166 3284 3977 4988 1166 Size: 2118 693 1011 4309 Fragments arranged by size: 4309 2118 1011 693 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4379 5252 1184 Size: 3195 873 4063 Fragments arranged by size: 4063 3195 873 AlwNI CAG_nnn'CTG Cuts at: 2459 2866 4018 4093 4594 4703 5125 5356 5874 Size: 407 1152 75 501 109 422 231 518 Cuts at: 5874 7446 2459 Size: 1572 3144 Fragments arranged by size: 3144 1572 1152 518 501 422 407 231 109 75 ApaI G_GGCC'C Cuts at: 2582 2716 3417 4318 4382 2582 Size: 134 701 901 64 6331 Fragments arranged by size: 6331 901 701 134 64 ApaLI G'TGCA_C Cuts at: 6188 7344 6188 Size: 1156 6975 ApoI r'AATT_y Cuts at: 1493 5440 5666 5818 7927 1493 Size: 3947 226 152 2109 1697 Fragments arranged by size: 3947 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6685 160 Size: 872 5653 1606 Fragments arranged by size: 5653 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8131 AvaI C'yCGr_G Cuts at: 1870 1943 2030 2180 2709 2715 4390 4436 5456 Size: 73 87 150 529 6 1675 46 1020 Cuts at: 5456 1870 Size: 4545 Fragments arranged by size: 4545 1675 1020 529 150 87 73 46 6 BamHI G'GATC_C Cuts at: 5046 5461 5828 5046 Size: 415 367 7349 Fragments arranged by size: 7349 415 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2036 2062 2836 3127 3804 Size: 325 217 627 249 26 774 291 677 Cuts at: 3804 3810 5299 5452 6907 618 Size: 6 1489 153 1455 1842 Fragments arranged by size: 1842 1489 1455 774 677 627 325 291 249 217 153 26 6 BanII G_rGCy'C Cuts at: 1391 2079 2469 2582 2600 2716 2759 3065 3417 Size: 688 390 113 18 116 43 306 352 Cuts at: 3417 3731 3773 4012 4087 4318 4382 4614 4799 Size: 314 42 239 75 231 64 232 185 Cuts at: 4799 4845 5450 1391 Size: 46 605 4072 Fragments arranged by size: 4072 688 605 390 352 314 306 239 232 231 185 116 113 75 64 46 43 42 18 BbeI G_GCGC'C Cuts at: 2040 3808 2040 Size: 1768 6363 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3938 4132 5192 928 Size: 971 2039 194 1060 3867 Fragments arranged by size: 3867 2039 1060 971 194 BbvCI CC'TCA_GC Cuts at: 2630 3273 3893 2630 Size: 643 620 6868 Fragments arranged by size: 6868 643 620 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6322 6356 1903 Size: 34 4385 34 3678 Fragments arranged by size: 4385 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5117 6023 7233 5117 Size: 906 1210 6015 Fragments arranged by size: 6015 1210 906 BclI T'GATC_A Cuts at: 1548 1548 Size: 8131 BfrBI ATG'CAT Cuts at: 5200 5200 Size: 8131 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3503 4157 4609 Size: 107 122 71 631 605 1831 654 452 Cuts at: 4609 5010 5308 6742 136 Size: 401 298 1434 1525 Fragments arranged by size: 1831 1525 1434 654 631 605 452 401 298 122 107 71 BglII A'GATC_T Cuts at: 4209 8126 4209 Size: 3917 4214 BlpI GC'TnA_GC Cuts at: 4011 5548 4011 Size: 1537 6594 Bme1580I G_kGCm'C Cuts at: 1790 2067 2582 2716 2795 2806 2841 3417 4318 Size: 277 515 134 79 11 35 576 901 Cuts at: 4318 4363 4382 4669 6192 7348 1790 Size: 45 19 287 1523 1156 2573 Fragments arranged by size: 2573 1523 1156 901 576 515 287 277 134 79 45 35 19 11 BmgBI CAC'GTC Cuts at: 1318 2095 5094 5190 1318 Size: 777 2999 96 4259 Fragments arranged by size: 4259 2999 777 96 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3819 3847 5862 Size: 416 53 83 399 24 2402 28 2015 Cuts at: 5862 6820 442 Size: 958 1753 Fragments arranged by size: 2402 2015 1753 958 416 399 83 53 28 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8131 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2365 2365 Size: 8131 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2421 2593 2773 2927 3049 3272 3380 4115 Size: 1182 172 180 154 122 223 108 735 Cuts at: 4115 4340 4364 6791 1239 Size: 225 24 2427 2579 Fragments arranged by size: 2579 2427 1182 735 225 223 180 172 154 122 108 24 Bpu10I CC'TnA_GC Cuts at: 2630 3273 3632 3893 5160 2630 Size: 643 359 261 1267 5601 Fragments arranged by size: 5601 1267 643 359 261 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3392 4775 6256 7121 7419 3392 Size: 1383 1481 865 298 4104 Fragments arranged by size: 4104 1481 1383 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4130 4241 6794 882 Size: 617 2631 111 2553 2219 Fragments arranged by size: 2631 2553 2219 617 111 BsaAI yAC'GTr Cuts at: 493 1204 2990 5922 493 Size: 711 1786 2932 2702 Fragments arranged by size: 2932 2702 1786 711 BsaBI GATnn'nnATC Cuts at: 8125 8125 Size: 8131 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2037 3186 3805 3987 Size: 53 83 186 936 504 1149 619 182 Cuts at: 3987 5938 6320 6955 275 Size: 1951 382 635 1451 Fragments arranged by size: 1951 1451 1149 936 635 619 504 382 186 182 83 53 BsaWI w'CCGG_w Cuts at: 4799 6564 6962 7236 7383 7794 4799 Size: 1765 398 274 147 411 5136 Fragments arranged by size: 5136 1765 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2338 2368 2388 2418 2774 Size: 30 165 30 935 30 20 30 356 Cuts at: 2774 2804 2959 2989 1178 Size: 30 155 30 6320 Fragments arranged by size: 6320 935 356 165 155 30 30 30 30 30 30 20 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 2114 2117 2346 2402 2432 2441 4706 Size: 901 27 3 229 56 30 9 2265 Cuts at: 4706 4811 1186 Size: 105 4506 Fragments arranged by size: 4506 2265 901 229 105 56 30 27 9 3 BseYI C'CCAG_C Cuts at: 2189 2601 2693 2702 2732 2866 2978 3104 3409 Size: 412 92 9 30 134 112 126 305 Cuts at: 3409 3416 3922 4003 4385 5118 5221 7334 7830 Size: 7 506 81 382 733 103 2113 496 Cuts at: 7830 2189 Size: 2490 Fragments arranged by size: 2490 2113 733 506 496 412 382 305 134 126 112 103 92 81 30 9 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3830 5402 1163 Size: 573 2094 1572 3892 Fragments arranged by size: 3892 2094 1572 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5581 6342 7370 1540 Size: 275 186 3580 761 1028 2301 Fragments arranged by size: 3580 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2624 3065 3873 3984 4012 4284 4995 5064 6192 Size: 441 808 111 28 272 711 69 1128 Cuts at: 6192 6277 7348 2624 Size: 85 1071 3407 Fragments arranged by size: 3407 1128 1071 808 711 441 272 111 85 69 28 BsmI GAATG_Cn' Cuts at: 5642 5735 5642 Size: 93 8038 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2079 2469 2582 2600 2624 2716 Size: 399 277 12 390 113 18 24 92 Cuts at: 2716 2759 2795 2806 2841 3065 3417 3731 3773 Size: 43 36 11 35 224 352 314 42 Cuts at: 3773 3873 3984 4012 4087 4284 4318 4363 4382 Size: 100 111 28 75 197 34 45 19 Cuts at: 4382 4614 4669 4799 4845 4995 5064 5450 6192 Size: 232 55 130 46 150 69 386 742 Cuts at: 6192 6277 7348 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 390 386 352 314 277 232 224 197 150 130 113 111 100 92 85 75 69 55 46 45 43 42 36 35 34 28 24 19 18 12 11 BspEI T'CCGG_A Cuts at: 7794 7794 Size: 8131 BspHI T'CATG_A Cuts at: 3487 6020 7750 3487 Size: 2533 1730 3868 Fragments arranged by size: 3868 2533 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2130 2784 2824 3603 3840 4824 5219 5486 Size: 1286 654 40 779 237 984 395 267 Cuts at: 5486 844 Size: 3489 Fragments arranged by size: 3489 1286 984 779 654 395 267 237 40 BsrBI CCG'CTC Cuts at: 1394 1823 2209 2257 4890 6018 1394 Size: 429 386 48 2633 1128 3507 Fragments arranged by size: 3507 2633 1128 429 386 48 BsrDI GCAATG_nn' Cuts at: 65 1248 3456 5311 6626 6800 65 Size: 1183 2208 1855 1315 174 1396 Fragments arranged by size: 2208 1855 1396 1315 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2226 2745 3513 3807 5143 6775 6962 Size: 110 416 519 768 294 1336 1632 187 Cuts at: 6962 1700 Size: 2869 Fragments arranged by size: 2869 1632 1336 768 519 416 294 187 110 BsrGI T'GTAC_A Cuts at: 96 5079 96 Size: 4983 3148 BssHII G'CGCG_C Cuts at: 1925 2264 1925 Size: 339 7792 BssSI C'ACGA_G Cuts at: 3440 4639 5006 6191 7203 3440 Size: 1199 367 1185 1012 4368 Fragments arranged by size: 4368 1199 1185 1012 367 BstAPI GCAn_nnn'nTGC Cuts at: 794 2619 2836 4288 794 Size: 1825 217 1452 4637 Fragments arranged by size: 4637 1825 1452 217 BstBI TT'CG_AA Cuts at: 6935 6935 Size: 8131 BstEII G'GTnAC_C Cuts at: 1184 3224 1184 Size: 2040 6091 BstXI CCAn_nnnn'nTGG Cuts at: 2656 3212 5008 5026 2656 Size: 556 1796 18 5761 Fragments arranged by size: 5761 1796 556 18 BstYI r'GATC_y Cuts at: 1972 3258 4209 4956 5046 5070 5461 5828 6213 Size: 1286 951 747 90 24 391 367 385 Cuts at: 6213 6230 7682 7768 7780 8126 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1286 951 747 391 385 367 346 90 86 24 17 12 Bsu36I CC'TnA_GG Cuts at: 2476 4308 2476 Size: 1832 6299 BtgI C'CryG_G Cuts at: 513 1065 2004 3109 3135 3279 3606 4682 4881 Size: 552 939 1105 26 144 327 1076 199 Cuts at: 4881 5256 513 Size: 375 3388 Fragments arranged by size: 3388 1105 1076 939 552 375 327 199 144 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4918 4981 5215 487 Size: 683 56 3692 63 234 3403 Fragments arranged by size: 3692 3403 683 234 63 56 BtsI GCAGTG_nn' Cuts at: 2806 4286 4363 4412 4669 5234 5647 6441 6461 Size: 1480 77 49 257 565 413 794 20 Cuts at: 6461 2806 Size: 4476 Fragments arranged by size: 4476 1480 794 565 413 257 77 49 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8131 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2800 2835 3016 3051 529 Size: 35 2236 35 181 35 5609 Fragments arranged by size: 5609 2236 181 35 35 35 DraI TTT'AAA Cuts at: 5435 5435 Size: 8131 DraIII CAC_nnn'GTG Cuts at: 2938 3683 2938 Size: 745 7386 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7138 809 Size: 507 5822 1802 Fragments arranged by size: 5822 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2763 3170 Size: 54 1425 50 275 18 177 756 407 Cuts at: 3170 3861 4108 4158 5395 5578 6467 8096 8 Size: 691 247 50 1237 183 889 1629 43 Fragments arranged by size: 1629 1425 1237 889 756 691 407 275 247 183 177 54 50 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5578 1537 Size: 275 3766 4090 Fragments arranged by size: 4090 3766 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2409 2418 2427 2888 3404 3878 3998 5336 Size: 1056 9 9 461 516 474 120 1338 Cuts at: 5336 6061 1353 Size: 725 3423 Fragments arranged by size: 3423 1338 1056 725 516 474 461 120 9 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2867 5209 6718 7092 7238 387 Size: 1064 138 1278 2342 1509 374 146 1280 Fragments arranged by size: 2342 1509 1280 1278 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3063 4010 3063 Size: 947 7184 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2421 2593 2773 2927 3023 3049 3263 Size: 393 789 172 180 154 96 26 214 Cuts at: 3263 3272 3285 3380 3765 4115 4340 4364 6194 Size: 9 13 95 385 350 225 24 1830 Cuts at: 6194 6791 7578 7846 1239 Size: 597 787 268 1524 Fragments arranged by size: 1830 1524 789 787 597 393 385 350 268 225 214 180 172 154 96 95 26 24 13 9 EcoNI CCTnn'n_nnAGG Cuts at: 2051 2051 Size: 8131 EcoO109I rG'GnC_Cy Cuts at: 2149 2176 2370 2481 3376 3413 4090 4314 4315 Size: 27 194 111 895 37 677 224 1 Cuts at: 4315 4326 4378 4379 4440 2149 Size: 11 52 1 61 5840 Fragments arranged by size: 5840 895 677 224 194 111 61 52 37 27 11 1 1 EcoRI G'AATT_C Cuts at: 5440 5818 5440 Size: 378 7753 FspI TGC'GCA Cuts at: 4466 6637 4466 Size: 2171 5960 HaeII r_GCGC'y Cuts at: 1678 2040 3454 3808 4639 4760 5357 7278 1678 Size: 362 1414 354 831 121 597 1921 2531 Fragments arranged by size: 2531 1921 1414 831 597 362 354 121 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2959 2991 4622 4654 6772 Size: 32 135 32 1387 32 1631 32 2118 Cuts at: 6772 6804 6846 6878 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2118 1631 1387 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4914 5475 5721 669 Size: 988 281 2976 561 246 3079 Fragments arranged by size: 3079 2976 988 561 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8131 HpaI GTT'AAC Cuts at: 5721 5721 Size: 8131 KasI G'GCGC_C Cuts at: 2036 3804 2036 Size: 1768 6363 KpnI G_GTAC'C Cuts at: 3814 5456 3814 Size: 1642 6489 MfeI C'AATT_G Cuts at: 5730 5730 Size: 8131 MluI A'CGCG_T Cuts at: 8007 8007 Size: 8131 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5024 7245 7429 1305 Size: 3719 2221 184 2007 Fragments arranged by size: 3719 2221 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 3172 5397 10 Size: 54 1425 343 177 1163 2225 2744 Fragments arranged by size: 2744 2225 1425 1163 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3284 3467 3977 4430 4544 4905 4988 Size: 648 2118 183 510 453 114 361 83 Cuts at: 4988 5024 5138 5426 6089 6448 6607 518 Size: 36 114 288 663 359 159 2042 Fragments arranged by size: 2118 2042 663 648 510 453 361 359 288 183 159 114 114 83 36 MspA1I CmG'CkG Cuts at: 1606 3866 3977 4018 4045 4703 4978 5113 5221 Size: 2260 111 41 27 658 275 135 108 Cuts at: 5221 5547 6224 7372 7617 1606 Size: 326 677 1148 245 2120 Fragments arranged by size: 2260 2120 1148 677 658 326 275 245 135 111 108 41 27 NaeI GCC'GGC Cuts at: 2747 5145 2747 Size: 2398 5733 NarI GG'CG_CC Cuts at: 2037 3805 2037 Size: 1768 6363 NcoI C'CATG_G Cuts at: 513 1065 2004 3109 3135 513 Size: 552 939 1105 26 5509 Fragments arranged by size: 5509 1105 939 552 26 NdeI CA'TA_TG Cuts at: 387 387 Size: 8131 NgoMIV G'CCGG_C Cuts at: 2745 5143 2745 Size: 2398 5733 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8131 NotI GC'GGCC_GC Cuts at: 5578 5578 Size: 8131 NsiI A_TGCA'T Cuts at: 5202 5202 Size: 8131 NspI r_CATG'y Cuts at: 1132 1229 3211 5425 5489 7034 1132 Size: 97 1982 2214 64 1545 2229 Fragments arranged by size: 2229 2214 1982 1545 97 64 PasI CC'CwG_GG Cuts at: 1348 2540 2806 3698 4657 4834 1348 Size: 1192 266 892 959 177 4645 Fragments arranged by size: 4645 1192 959 892 266 177 PciI A'CATG_T Cuts at: 7030 7030 Size: 8131 PflMI CCAn_nnn'nTGG Cuts at: 1108 2866 3859 4093 1108 Size: 1758 993 234 5146 Fragments arranged by size: 5146 1758 993 234 PfoI T'CCnGG_A Cuts at: 2382 2951 3357 3546 4959 5334 2382 Size: 569 406 189 1413 375 5179 Fragments arranged by size: 5179 1413 569 406 375 189 PmeI GTTT'AAAC Cuts at: 5435 5435 Size: 8131 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6200 6232 7713 7745 6200 Size: 32 1481 32 6586 Fragments arranged by size: 6586 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2149 2176 2370 2481 3376 4090 4326 4440 2149 Size: 27 194 111 895 714 236 114 5840 Fragments arranged by size: 5840 895 714 236 194 114 111 27 PshAI GACnn'nnGTC Cuts at: 1613 3662 1613 Size: 2049 6082 PsiI TTA'TAA Cuts at: 5701 5701 Size: 8131 PspOMI G'GGCC_C Cuts at: 2578 2712 3413 4314 4378 2578 Size: 134 701 901 64 6331 Fragments arranged by size: 6331 901 701 134 64 PstI C_TGCA'G Cuts at: 830 2878 3325 5352 5483 830 Size: 2048 447 2027 131 3478 Fragments arranged by size: 3478 2048 2027 447 131 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8131 PvuII CAG'CTG Cuts at: 5113 5221 5113 Size: 108 8023 RsrII CG'GwC_CG Cuts at: 3388 4885 3388 Size: 1497 6634 SacI G_AGCT'C Cuts at: 3065 4012 3065 Size: 947 7184 SalI G'TCGA_C Cuts at: 1936 5473 1936 Size: 3537 4594 SanDI GG'GwC_CC Cuts at: 2176 2370 2481 4326 2176 Size: 194 111 1845 5981 Fragments arranged by size: 5981 1845 194 111 SapI GCTCTTCn'nnn_ Cuts at: 3878 5336 3878 Size: 1458 6673 SbfI CC_TGCA'GG Cuts at: 5483 5483 Size: 8131 ScaI AGT'ACT Cuts at: 6379 8091 6379 Size: 1712 6419 SfcI C'TryA_G Cuts at: 826 1045 2200 2874 3321 4605 5348 5479 6614 Size: 219 1155 674 447 1284 743 131 1135 Cuts at: 6614 7295 7486 826 Size: 681 191 1471 Fragments arranged by size: 1471 1284 1155 1135 743 681 674 447 219 191 131 SfoI GGC'GCC Cuts at: 2038 3806 2038 Size: 1768 6363 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8131 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8131 SmaI CCC'GGG Cuts at: 1872 2032 2711 2717 5458 1872 Size: 160 679 6 2741 4545 Fragments arranged by size: 4545 2741 679 160 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 3371 4790 6235 7136 7398 820 Size: 197 926 1428 1419 1445 901 262 1553 Fragments arranged by size: 1553 1445 1428 1419 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2990 5922 493 Size: 2497 2932 2702 Fragments arranged by size: 2932 2702 2497 SpeI A'CTAG_T Cuts at: 152 152 Size: 8131 SphI G_CATG'C Cuts at: 1132 5489 1132 Size: 4357 3774 SrfI GCCC'GGGC Cuts at: 2717 2717 Size: 8131 SspI AAT'ATT Cuts at: 5 52 6055 5 Size: 47 6003 2081 Fragments arranged by size: 6003 2081 47 StuI AGG'CCT Cuts at: 1517 2535 1517 Size: 1018 7113 StyI C'CwwG_G Cuts at: 513 1065 2004 2067 2127 2672 3109 3135 3189 Size: 552 939 63 60 545 437 26 54 Cuts at: 3189 5178 5570 513 Size: 1989 392 3074 Fragments arranged by size: 3074 1989 939 552 545 437 392 63 60 54 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2261 3111 3950 4217 4299 4913 5233 Size: 1255 681 850 839 267 82 614 320 Cuts at: 5233 6158 6175 6328 6513 6958 325 Size: 925 17 153 185 445 1498 Fragments arranged by size: 1498 1255 925 850 839 681 614 445 320 267 185 153 82 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3352 5079 5406 Size: 274 80 33 51 1443 1375 1727 327 Cuts at: 5406 6377 8089 96 Size: 971 1712 138 Fragments arranged by size: 1727 1712 1443 1375 971 327 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2388 2420 2774 2806 8091 8123 2388 Size: 32 354 32 5285 32 2396 Fragments arranged by size: 5285 2396 354 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8131 XbaI T'CTAG_A Cuts at: 5467 5467 Size: 8131 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3116 3165 4540 4546 3116 Size: 49 1375 6 6701 Fragments arranged by size: 6701 1375 49 6 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8131 XmaI C'CCGG_G Cuts at: 1870 2030 2709 2715 5456 1870 Size: 160 679 6 2741 4545 Fragments arranged by size: 4545 2741 679 160 6 XmnI GAAnn'nnTTC Cuts at: 6260 6260 Size: 8131 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5939 276 Size: 53 83 186 936 4405 2468 Fragments arranged by size: 4405 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NruI PacI PmlI PspXI PsrI SacII SexAI SfiI SwaI