Restriction map for
pFN21AB3864
(Linear) (Six-base) MAPSORT of: KIBB3864.seq Check: 6480 from: 1 to: 3426
KIBB3864.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 819 3214 3426
Size: 819 2395 212
Fragments arranged by size:
2395 819 212
Acc65I G'GTAC_C
Cuts at: 0 1805 3426
Size: 1805 1621
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1018 1258 1280 1760 3426
Size: 1018 240 22 480 1666
Fragments arranged by size:
1666 1018 480 240 22
AfeI AGC'GCT
Cuts at: 0 2632 3426
Size: 2632 794
AleI CACnn'nnGTG
Cuts at: 0 1279 1972 2983 3426
Size: 1279 693 1011 443
Fragments arranged by size:
1279 1011 693 443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2374 3247 3426
Size: 2374 873 179
Fragments arranged by size:
2374 873 179
AlwNI CAG_nnn'CTG
Cuts at: 0 454 861 2013 2088 2589 2698 3120 3351
Size: 454 407 1152 75 501 109 422 231
Cuts at: 3351 3426
Size: 75
Fragments arranged by size:
1152 501 454 422 407 231 109 75
75
ApaI G_GGCC'C
Cuts at: 0 577 711 1412 2313 2377 3426
Size: 577 134 701 901 64 1049
Fragments arranged by size:
1049 901 701 577 134 64
AvaI C'yCGr_G
Cuts at: 0 25 175 704 710 2385 2431 3426
Size: 25 150 529 6 1675 46 995
Fragments arranged by size:
1675 995 529 150 46 25 6
BamHI G'GATC_C
Cuts at: 0 3041 3426
Size: 3041 385
BanI G'GyrC_C
Cuts at: 0 31 57 831 1122 1799 1805 3294 3426
Size: 31 26 774 291 677 6 1489 132
Fragments arranged by size:
1489 774 677 291 132 31 26 6
BanII G_rGCy'C
Cuts at: 0 74 464 577 595 711 754 1060 1412
Size: 74 390 113 18 116 43 306 352
Cuts at: 1412 1726 1768 2007 2082 2313 2377 2609 2794
Size: 314 42 239 75 231 64 232 185
Cuts at: 2794 2840 3426
Size: 46 586
Fragments arranged by size:
586 390 352 314 306 239 232 231
185 116 113 75 74 64 46 43
42 18
BbeI G_GCGC'C
Cuts at: 0 35 1803 3426
Size: 35 1768 1623
Fragments arranged by size:
1768 1623 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 1933 2127 3187 3426
Size: 1933 194 1060 239
Fragments arranged by size:
1933 1060 239 194
BbvCI CC'TCA_GC
Cuts at: 0 625 1268 1888 3426
Size: 625 643 620 1538
Fragments arranged by size:
1538 643 625 620
BciVI GTATCCnnnnn_n'
Cuts at: 0 3112 3426
Size: 3112 314
BfrBI ATG'CAT
Cuts at: 0 3195 3426
Size: 3195 231
BglI GCCn_nnn'nGGC
Cuts at: 0 1498 2152 2604 3005 3303 3426
Size: 1498 654 452 401 298 123
Fragments arranged by size:
1498 654 452 401 298 123
BglII A'GATC_T
Cuts at: 0 2204 3426
Size: 2204 1222
BlpI GC'TnA_GC
Cuts at: 0 2006 3426
Size: 2006 1420
Bme1580I G_kGCm'C
Cuts at: 0 62 577 711 790 801 836 1412 2313
Size: 62 515 134 79 11 35 576 901
Cuts at: 2313 2358 2377 2664 3426
Size: 45 19 287 762
Fragments arranged by size:
901 762 576 515 287 134 79 62
45 35 19 11
BmgBI CAC'GTC
Cuts at: 0 90 3089 3185 3426
Size: 90 2999 96 241
Fragments arranged by size:
2999 241 96 90
BmrI ACTGGGnnnn_n'
Cuts at: 0 1814 1842 3426
Size: 1814 28 1584
Fragments arranged by size:
1814 1584 28
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 360 3426
Size: 360 3066
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 588 768 922 1044 1267 1375 2110
Size: 416 172 180 154 122 223 108 735
Cuts at: 2110 2335 2359 3426
Size: 225 24 1067
Fragments arranged by size:
1067 735 416 225 223 180 172 154
122 108 24
Bpu10I CC'TnA_GC
Cuts at: 0 625 1268 1627 1888 3155 3426
Size: 625 643 359 261 1267 271
Fragments arranged by size:
1267 643 625 359 271 261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1387 2770 3426
Size: 1387 1383 656
Fragments arranged by size:
1387 1383 656
BsaI GGTCTCn'nnnn_
Cuts at: 0 2125 2236 3426
Size: 2125 111 1190
Fragments arranged by size:
2125 1190 111
BsaAI yAC'GTr
Cuts at: 0 985 3426
Size: 985 2441
BsaHI Gr'CG_yC
Cuts at: 0 32 1181 1800 1982 3426
Size: 32 1149 619 182 1444
Fragments arranged by size:
1444 1149 619 182 32
BsaWI w'CCGG_w
Cuts at: 0 2794 3426
Size: 2794 632
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 333 363 383 413 769 799 954 984
Size: 333 30 20 30 356 30 155 30
Cuts at: 984 3426
Size: 2442
Fragments arranged by size:
2442 356 333 155 30 30 30 30
20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 82 109 112 341 397 427 436 2701
Size: 82 27 3 229 56 30 9 2265
Cuts at: 2701 2806 3426
Size: 105 620
Fragments arranged by size:
2265 620 229 105 82 56 30 27
9 3
BseYI C'CCAG_C
Cuts at: 0 184 596 688 697 727 861 973 1099
Size: 184 412 92 9 30 134 112 126
Cuts at: 1099 1404 1411 1917 1998 2380 3113 3216 3426
Size: 305 7 506 81 382 733 103 210
Fragments arranged by size:
733 506 412 382 305 210 184 134
126 112 103 92 81 30 9 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1825 3397 3426
Size: 1825 1572 29
Fragments arranged by size:
1825 1572 29
BsiHKAI G_wGCw'C
Cuts at: 0 619 1060 1868 1979 2007 2279 2990 3059
Size: 619 441 808 111 28 272 711 69
Cuts at: 3059 3426
Size: 367
Fragments arranged by size:
808 711 619 441 367 272 111 69
28
Bsp1286I G_dGCh'C
Cuts at: 0 62 74 464 577 595 619 711 754
Size: 62 12 390 113 18 24 92 43
Cuts at: 754 790 801 836 1060 1412 1726 1768 1868
Size: 36 11 35 224 352 314 42 100
Cuts at: 1868 1979 2007 2082 2279 2313 2358 2377 2609
Size: 111 28 75 197 34 45 19 232
Cuts at: 2609 2664 2794 2840 2990 3059 3426
Size: 55 130 46 150 69 367
Fragments arranged by size:
390 367 352 314 232 224 197 150
130 113 111 100 92 75 69 62
55 46 45 43 42 36 35 34
28 24 19 18 12 11
BspHI T'CATG_A
Cuts at: 0 1482 3426
Size: 1482 1944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 125 779 819 1598 1835 2819 3214 3426
Size: 125 654 40 779 237 984 395 212
Fragments arranged by size:
984 779 654 395 237 212 125 40
BsrBI CCG'CTC
Cuts at: 0 204 252 2885 3426
Size: 204 48 2633 541
Fragments arranged by size:
2633 541 204 48
BsrDI GCAATG_nn'
Cuts at: 0 1451 3306 3426
Size: 1451 1855 120
Fragments arranged by size:
1855 1451 120
BsrFI r'CCGG_y
Cuts at: 0 221 740 1508 1802 3138 3426
Size: 221 519 768 294 1336 288
Fragments arranged by size:
1336 768 519 294 288 221
BsrGI T'GTAC_A
Cuts at: 0 3074 3426
Size: 3074 352
BssHII G'CGCG_C
Cuts at: 0 259 3426
Size: 259 3167
BssSI C'ACGA_G
Cuts at: 0 1435 2634 3001 3426
Size: 1435 1199 367 425
Fragments arranged by size:
1435 1199 425 367
BstAPI GCAn_nnn'nTGC
Cuts at: 0 614 831 2283 3426
Size: 614 217 1452 1143
Fragments arranged by size:
1452 1143 614 217
BstEII G'GTnAC_C
Cuts at: 0 1219 3426
Size: 1219 2207
BstXI CCAn_nnnn'nTGG
Cuts at: 0 651 1207 3003 3021 3426
Size: 651 556 1796 18 405
Fragments arranged by size:
1796 651 556 405 18
BstYI r'GATC_y
Cuts at: 0 1253 2204 2951 3041 3065 3426
Size: 1253 951 747 90 24 361
Fragments arranged by size:
1253 951 747 361 90 24
Bsu36I CC'TnA_GG
Cuts at: 0 471 2303 3426
Size: 471 1832 1123
Fragments arranged by size:
1832 1123 471
BtgI C'CryG_G
Cuts at: 0 1104 1130 1274 1601 2677 2876 3251 3426
Size: 1104 26 144 327 1076 199 375 175
Fragments arranged by size:
1104 1076 375 327 199 175 144 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2913 2976 3210 3426
Size: 2913 63 234 216
Fragments arranged by size:
2913 234 216 63
BtsI GCAGTG_nn'
Cuts at: 0 801 2281 2358 2407 2664 3229 3426
Size: 801 1480 77 49 257 565 197
Fragments arranged by size:
1480 801 565 257 197 77 49
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 795 830 1011 1046 3426
Size: 795 35 181 35 2380
Fragments arranged by size:
2380 795 181 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 933 1678 3426
Size: 933 745 1748
Fragments arranged by size:
1748 933 745
EaeI y'GGCC_r
Cuts at: 0 2 758 1165 1856 2103 2153 3390 3426
Size: 2 756 407 691 247 50 1237 36
Fragments arranged by size:
1237 756 691 407 247 50 36 2
EarI CTCTTCn'nnn_
Cuts at: 0 404 413 422 883 1399 1873 1993 3331
Size: 404 9 9 461 516 474 120 1338
Cuts at: 3331 3426
Size: 95
Fragments arranged by size:
1338 516 474 461 404 120 95 9
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 862 3204 3426
Size: 862 2342 222
Fragments arranged by size:
2342 862 222
EcoICRI GAG'CTC
Cuts at: 0 1058 2005 3426
Size: 1058 947 1421
Fragments arranged by size:
1421 1058 947
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 588 768 922 1018 1044 1258 1267
Size: 416 172 180 154 96 26 214 9
Cuts at: 1267 1280 1375 1760 2110 2335 2359 3426
Size: 13 95 385 350 225 24 1067
Fragments arranged by size:
1067 416 385 350 225 214 180 172
154 96 95 26 24 13 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 46 3426
Size: 46 3380
EcoO109I rG'GnC_Cy
Cuts at: 0 144 171 365 476 1371 1408 2085 2309
Size: 144 27 194 111 895 37 677 224
Cuts at: 2309 2310 2321 2373 2374 2435 3426
Size: 1 11 52 1 61 991
Fragments arranged by size:
991 895 677 224 194 144 111 61
52 37 27 11 1 1
FspI TGC'GCA
Cuts at: 0 2461 3426
Size: 2461 965
HaeII r_GCGC'y
Cuts at: 0 35 1449 1803 2634 2755 3352 3426
Size: 35 1414 354 831 121 597 74
Fragments arranged by size:
1414 831 597 354 121 74 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 954 986 2617 2649 3426
Size: 954 32 1631 32 777
Fragments arranged by size:
1631 954 777 32 32
HincII GTy'rAC
Cuts at: 0 2909 3426
Size: 2909 517
KasI G'GCGC_C
Cuts at: 0 31 1799 3426
Size: 31 1768 1627
Fragments arranged by size:
1768 1627 31
KpnI G_GTAC'C
Cuts at: 0 1809 3426
Size: 1809 1617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3019 3426
Size: 3019 407
MscI TGG'CCA
Cuts at: 0 4 1167 3392 3426
Size: 4 1163 2225 34
Fragments arranged by size:
2225 1163 34 4
MslI CAynn'nnrTG
Cuts at: 0 1279 1462 1972 2425 2539 2900 2983 3019
Size: 1279 183 510 453 114 361 83 36
Cuts at: 3019 3133 3421 3426
Size: 114 288 5
Fragments arranged by size:
1279 510 453 361 288 183 114 114
83 36 5
MspA1I CmG'CkG
Cuts at: 0 1861 1972 2013 2040 2698 2973 3108 3216
Size: 1861 111 41 27 658 275 135 108
Cuts at: 3216 3426
Size: 210
Fragments arranged by size:
1861 658 275 210 135 111 108 41
27
NaeI GCC'GGC
Cuts at: 0 742 3140 3426
Size: 742 2398 286
Fragments arranged by size:
2398 742 286
NarI GG'CG_CC
Cuts at: 0 32 1800 3426
Size: 32 1768 1626
Fragments arranged by size:
1768 1626 32
NcoI C'CATG_G
Cuts at: 0 1104 1130 3426
Size: 1104 26 2296
Fragments arranged by size:
2296 1104 26
NgoMIV G'CCGG_C
Cuts at: 0 740 3138 3426
Size: 740 2398 288
Fragments arranged by size:
2398 740 288
NsiI A_TGCA'T
Cuts at: 0 3197 3426
Size: 3197 229
NspI r_CATG'y
Cuts at: 0 1206 3420 3426
Size: 1206 2214 6
Fragments arranged by size:
2214 1206 6
PasI CC'CwG_GG
Cuts at: 0 535 801 1693 2652 2829 3426
Size: 535 266 892 959 177 597
Fragments arranged by size:
959 892 597 535 266 177
PflMI CCAn_nnn'nTGG
Cuts at: 0 861 1854 2088 3426
Size: 861 993 234 1338
Fragments arranged by size:
1338 993 861 234
PfoI T'CCnGG_A
Cuts at: 0 377 946 1352 1541 2954 3329 3426
Size: 377 569 406 189 1413 375 97
Fragments arranged by size:
1413 569 406 377 375 189 97
PpuMI rG'GwC_Cy
Cuts at: 0 144 171 365 476 1371 2085 2321 2435
Size: 144 27 194 111 895 714 236 114
Cuts at: 2435 3426
Size: 991
Fragments arranged by size:
991 895 714 236 194 144 114 111
27
PshAI GACnn'nnGTC
Cuts at: 0 1657 3426
Size: 1657 1769
PspOMI G'GGCC_C
Cuts at: 0 573 707 1408 2309 2373 3426
Size: 573 134 701 901 64 1053
Fragments arranged by size:
1053 901 701 573 134 64
PstI C_TGCA'G
Cuts at: 0 873 1320 3347 3426
Size: 873 447 2027 79
Fragments arranged by size:
2027 873 447 79
PvuII CAG'CTG
Cuts at: 0 3108 3216 3426
Size: 3108 108 210
Fragments arranged by size:
3108 210 108
RsrII CG'GwC_CG
Cuts at: 0 1383 2880 3426
Size: 1383 1497 546
Fragments arranged by size:
1497 1383 546
SacI G_AGCT'C
Cuts at: 0 1060 2007 3426
Size: 1060 947 1419
Fragments arranged by size:
1419 1060 947
SanDI GG'GwC_CC
Cuts at: 0 171 365 476 2321 3426
Size: 171 194 111 1845 1105
Fragments arranged by size:
1845 1105 194 171 111
SapI GCTCTTCn'nnn_
Cuts at: 0 1873 3331 3426
Size: 1873 1458 95
Fragments arranged by size:
1873 1458 95
SfcI C'TryA_G
Cuts at: 0 195 869 1316 2600 3343 3426
Size: 195 674 447 1284 743 83
Fragments arranged by size:
1284 743 674 447 195 83
SfoI GGC'GCC
Cuts at: 0 33 1801 3426
Size: 33 1768 1625
Fragments arranged by size:
1768 1625 33
SmaI CCC'GGG
Cuts at: 0 27 706 712 3426
Size: 27 679 6 2714
Fragments arranged by size:
2714 679 27 6
SmlI C'TyrA_G
Cuts at: 0 1366 2785 3426
Size: 1366 1419 641
Fragments arranged by size:
1419 1366 641
SnaBI TAC'GTA
Cuts at: 0 985 3426
Size: 985 2441
SrfI GCCC'GGGC
Cuts at: 0 712 3426
Size: 712 2714
StuI AGG'CCT
Cuts at: 0 530 3426
Size: 530 2896
StyI C'CwwG_G
Cuts at: 0 62 122 667 1104 1130 1184 3173 3426
Size: 62 60 545 437 26 54 1989 253
Fragments arranged by size:
1989 545 437 253 62 60 54 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 256 1106 1945 2212 2294 2908 3228 3426
Size: 256 850 839 267 82 614 320 198
Fragments arranged by size:
850 839 614 320 267 256 198 82
TatI w'GTAC_w
Cuts at: 0 1347 3074 3401 3426
Size: 1347 1727 327 25
Fragments arranged by size:
1727 1347 327 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 383 415 769 801 3426
Size: 383 32 354 32 2625
Fragments arranged by size:
2625 383 354 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1111 1160 2535 2541 3426
Size: 1111 49 1375 6 885
Fragments arranged by size:
1375 1111 885 49 6
XmaI C'CCGG_G
Cuts at: 0 25 704 710 3426
Size: 25 679 6 2716
Fragments arranged by size:
2716 679 25 6
Enzymes that do cut:
AarI Acc65I AcuI AfeI AleI AlfI AlwNI ApaI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PflMI
PfoI PpuMI PshAI PspOMI PstI PvuII RsrII SacI
SanDI SapI SfcI SfoI SmaI SmlI SnaBI SrfI
StuI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AhdI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BcgI
BclI BmtI BsaBI BsiEI BsiWI BsmI BsmBI BspEI
BstBI BstZ17I ClaI DraI DrdI EagI EcoRI EcoRV
FalI FseI FspAI HindIII HpaI MfeI MluI NdeI
NheI NotI NruI PacI PciI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI SacII SalI SbfI ScaI
SexAI SfiI SgfI SgrAI SpeI SphI SspI SwaI
Tth111I XbaI XhoI XmnI ZraI