Restriction map for
pFN21AB3863
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII BamHI
BbeI BbrPI BcgI BciVI BclI
BfrI BglII BsaBI BsaHI BsaI
BseAI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI BstAPI BstEII
BstPI BstUI BstXI BstYI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AocI 922 CCTAAGG CC^T[ACGT]AGG
ApaI 66 GGGCCC GGGCC^C
AvrII 1726 CCTAGG C^CTAGG
BbvCI 1185 CCTCAGC CCTCAGC(-5/-2)
BlnI 1726 CCTAGG C^CTAGG
BsaWI 330 ACCGGA [AT]^CCGG[AT]
BsmBI 651 CGTCTC CGTCTC(1/5)
BsmFI 1611 GTCCC GGGAC(10/14)
BsmI 45 GAATGC GAATGC(1/-1)
BspMI 1872 ACCTGC ACCTGC(4/8)
BsrBI 778 CCGCTC CCGCTC(-3/-3)
Bst1107I 678 GTATAC GTA^TAC
BstBI 98 TTCGAA TT^CGAA
BstZ17I 678 GTATAC GTA^TAC
Bsu36I 922 CCTAAGG CC^T[ACGT]AGG
DpnII 1294 GATC ^GATC
DsaI 1178 CCATGG C^C[GA][CT]GG
Eco81I 922 CCTAAGG CC^T[ACGT]AGG
HindIII 1033 AAGCTT A^AGCTT
HphI 446 TCACC GGTGA(8/7)
MboI 1294 GATC ^GATC
MslI 1054 CATTGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1178 CCATGG C^CATGG
NdeII 1294 GATC ^GATC
NheI 898 GCTAGC G^CTAGC
NspI 1092 GCATGC [GA]CATG^[CT]
NspV 98 TTCGAA TT^CGAA
PstI 794 CTGCAG CTGCA^G
Sau3AI 1294 GATC ^GATC
SfuI 98 TTCGAA TT^CGAA
SnaBI 2120 TACGTA TAC^GTA
SphI 1092 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 326 GTCTAC GT^[AC][GT]AC
678 GTATAC
Asp700I 1031 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1573 GAACAGCTTC
AspHI 1017 GAGCTC G[AT]GC[AT]^C
1464 GAGCTC
BalI 289 TGGCCA TGG^CCA
1418 TGGCCA
BbsI 1428 GTCTTC GAAGAC(2/6)
2373 GTCTTC
BlpI 1556 GCTTAGC GC^T[ACGT]AGC
1947 GCTGAGC
Bpu1102I 1556 GCTTAGC GC^T[ACGT]AGC
1947 GCTGAGC
BpuAI 1428 GTCTTC GAAGAC(2/6)
2373 GTCTTC
BsaAI 2027 TACGTG [CT]AC^GT[GA]
2120 TACGTA
BsgI 1136 CTGCAC GTGCAG(16/14)
2294 CTGCAC
BsiHKAI 1017 GAGCTC G[AT]GC[AT]^C
1464 GAGCTC
CelII 1556 GCTTAGC GC^T[ACGT]AGC
1947 GCTGAGC
EaeI 289 TGGCCA [CT]^GGCC[GA]
1418 TGGCCA
EcoT14I 1178 CCATGG C^C[AT][AT]GG
1726 CCTAGG
MluNI 289 TGGCCA TGG^CCA
1418 TGGCCA
MscI 289 TGGCCA TGG^CCA
1418 TGGCCA
PvuII 232 CAGCTG CAG^CTG
1945 CAGCTG
SacI 1017 GAGCTC GAGCT^C
1464 GAGCTC
StyI 1178 CCATGG C^C[AT][AT]GG
1726 CCTAGG
TaqI 99 TCGA T^CGA
1689 TCGA
TthHB8I 99 TCGA T^CGA
1689 TCGA
XmnI 1031 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1573 GAACAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI Aor51HI AvaII
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DraII EarI
Eco47III EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeI MaeII MaeIII MboII
MnlI MseI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PleI
RsaI Sau96I ScrFI SfaNI SfcI
SmlI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI