(Circular) (Six-base) MAPSORT of: pFN21AB3863.seq Check: 2479 from: 1 to: 7108 pFN21AB3863 7108 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3886 3886 Size: 7108 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4918 278 Size: 53 83 186 936 3382 2468 Fragments arranged by size: 3382 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2332 2684 4451 1937 Size: 395 352 1767 4594 Fragments arranged by size: 4594 1767 395 352 Acc65I G'GTAC_C Cuts at: 4429 4429 Size: 7108 AclI AA'CG_TT Cuts at: 1563 5235 5608 1563 Size: 3672 373 3063 Fragments arranged by size: 3672 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2403 3420 4365 5171 6555 6823 1632 Size: 771 1017 945 806 1384 268 1917 Fragments arranged by size: 1917 1384 1017 945 806 771 268 AfeI AGC'GCT Cuts at: 2616 4106 4330 2616 Size: 1490 224 5394 Fragments arranged by size: 5394 1490 224 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6911 AflIII A'CryG_T Cuts at: 1203 6007 6984 1203 Size: 4804 977 1327 Fragments arranged by size: 4804 1327 977 AgeI A'CCGG_T Cuts at: 5939 5939 Size: 7108 AhdI GACnn_n'nnGTC Cuts at: 5837 5837 Size: 7108 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7108 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3011 1184 Size: 1827 5281 AlwNI CAG_nnn'CTG Cuts at: 4851 6423 4851 Size: 1572 5536 ApaI G_GGCC'C Cuts at: 2075 2075 Size: 7108 ApaLI G'TGCA_C Cuts at: 5165 6321 5165 Size: 1156 5952 ApoI r'AATT_y Cuts at: 1493 4417 4643 4795 6904 1493 Size: 2924 226 152 2109 1697 Fragments arranged by size: 2924 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5662 160 Size: 872 4630 1606 Fragments arranged by size: 4630 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7108 AvaI C'yCGr_G Cuts at: 1870 1943 4433 1870 Size: 73 2490 4545 Fragments arranged by size: 4545 2490 73 AvrII C'CTAG_G Cuts at: 3731 3731 Size: 7108 BamHI G'GATC_C Cuts at: 4438 4805 4438 Size: 367 6741 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2155 2647 3736 3839 4429 Size: 325 217 627 368 492 1089 103 590 Cuts at: 4429 5884 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1089 627 590 492 368 325 217 103 BanII G_rGCy'C Cuts at: 1391 2075 2141 3026 3473 3556 4026 4427 1391 Size: 684 66 885 447 83 470 401 4072 Fragments arranged by size: 4072 885 684 470 447 401 83 66 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3426 4371 928 Size: 971 1527 945 3665 Fragments arranged by size: 3665 1527 971 945 BbvCI CC'TCA_GC Cuts at: 3191 3191 Size: 7108 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5299 5333 1903 Size: 34 50 34 3278 34 3678 Fragments arranged by size: 3678 3278 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5000 6210 5000 Size: 1210 5898 BclI T'GATC_A Cuts at: 1548 1548 Size: 7108 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2302 2609 2885 Size: 107 122 71 631 605 630 307 276 Cuts at: 2885 3813 5719 136 Size: 928 1906 1525 Fragments arranged by size: 1906 1525 928 631 630 605 307 276 122 107 71 BglII A'GATC_T Cuts at: 7103 7103 Size: 7108 BlpI GC'TnA_GC Cuts at: 3562 3953 4525 3562 Size: 391 572 6145 Fragments arranged by size: 6145 572 391 Bme1580I G_kGCm'C Cuts at: 1790 2075 2641 3739 5169 6325 1790 Size: 285 566 1098 1430 1156 2573 Fragments arranged by size: 2573 1430 1156 1098 566 285 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7108 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2336 4839 5797 Size: 416 53 83 399 24 919 2503 958 Cuts at: 5797 442 Size: 1753 Fragments arranged by size: 2503 1753 958 919 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2907 1056 Size: 1851 5257 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3103 3490 4312 3103 Size: 387 822 5899 Fragments arranged by size: 5899 822 387 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2603 2793 2883 3430 3849 4324 5768 1239 Size: 1364 190 90 547 419 475 1444 2579 Fragments arranged by size: 2579 1444 1364 547 475 419 190 90 Bpu10I CC'TnA_GC Cuts at: 3191 4325 3191 Size: 1134 5974 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2741 2942 4061 4121 5233 6098 6396 2741 Size: 201 1119 60 1112 865 298 3453 Fragments arranged by size: 3453 1119 1112 865 298 201 60 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5771 882 Size: 617 4272 2219 Fragments arranged by size: 4272 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4034 4127 4899 493 Size: 711 2830 93 772 2702 Fragments arranged by size: 2830 2702 772 711 93 BsaBI GATnn'nnATC Cuts at: 7102 7102 Size: 7108 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4915 5297 5932 275 Size: 53 83 186 936 3382 382 635 1451 Fragments arranged by size: 3382 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2335 5541 5939 6213 6360 6771 2335 Size: 3206 398 274 147 411 2672 Fragments arranged by size: 3206 2672 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2769 2788 2799 2818 1178 Size: 30 165 30 1366 19 11 19 5468 Fragments arranged by size: 5468 1366 165 30 30 19 19 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2401 2679 2796 3463 3500 3503 3515 3524 Size: 1215 278 117 667 37 3 12 9 Cuts at: 3524 3920 3923 4348 4351 1186 Size: 396 3 425 3 3943 Fragments arranged by size: 3943 1215 667 425 396 278 117 37 12 9 3 3 3 BseYI C'CCAG_C Cuts at: 2062 2068 2814 3704 3988 6311 6807 2062 Size: 6 746 890 284 2323 496 2363 Fragments arranged by size: 2363 2323 890 746 496 284 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3126 4284 1163 Size: 573 1390 1158 3987 Fragments arranged by size: 3987 1390 1158 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4558 5319 6347 1540 Size: 275 186 2557 761 1028 2301 Fragments arranged by size: 2557 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3026 3473 5169 5254 6325 3026 Size: 447 1696 85 1071 3809 Fragments arranged by size: 3809 1696 1071 447 85 BsmI GAATG_Cn' Cuts at: 2056 4619 4712 2056 Size: 2563 93 4452 Fragments arranged by size: 4452 2563 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2662 2662 Size: 7108 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 2141 2641 3026 3473 3556 3739 Size: 399 285 66 500 385 447 83 183 Cuts at: 3739 4026 4427 5169 5254 6325 1391 Size: 287 401 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 500 447 401 399 385 287 285 183 85 83 66 BspEI T'CCGG_A Cuts at: 6771 6771 Size: 7108 BspHI T'CATG_A Cuts at: 4997 6727 4997 Size: 1730 5378 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3886 4463 844 Size: 3042 577 3489 Fragments arranged by size: 3489 3042 577 BsrBI CCG'CTC Cuts at: 1394 1823 2785 4995 1394 Size: 429 962 2210 3507 Fragments arranged by size: 3507 2210 962 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3058 3070 3321 5603 5777 65 Size: 1183 1810 12 251 2282 174 1396 Fragments arranged by size: 2282 1810 1396 1183 251 174 12 BsrFI r'CCGG_y Cuts at: 1700 1810 5752 5939 1700 Size: 110 3942 187 2869 Fragments arranged by size: 3942 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7108 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7108 BssSI C'ACGA_G Cuts at: 5168 6180 5168 Size: 1012 6096 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7108 BstBI TT'CG_AA Cuts at: 2104 5912 2104 Size: 3808 3300 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7108 BstYI r'GATC_y Cuts at: 1972 4438 4805 5190 5207 6659 6745 6757 7103 Size: 2466 367 385 17 1452 86 12 346 Cuts at: 7103 1972 Size: 1977 Fragments arranged by size: 2466 1977 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2685 2685 Size: 7108 Bsu36I CC'TnA_GG Cuts at: 2928 2928 Size: 7108 BtgI C'CryG_G Cuts at: 513 1065 3183 513 Size: 552 2118 4438 Fragments arranged by size: 4438 2118 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3542 487 Size: 683 56 2316 4053 Fragments arranged by size: 4053 2316 683 56 BtsI GCAGTG_nn' Cuts at: 2632 3469 3787 3799 4320 4624 5418 5438 2632 Size: 837 318 12 521 304 794 20 4302 Fragments arranged by size: 4302 837 794 521 318 304 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7108 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7073 DraI TTT'AAA Cuts at: 4412 4412 Size: 7108 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6115 809 Size: 507 4799 1802 Fragments arranged by size: 4799 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2294 3423 4555 Size: 54 1425 50 275 18 464 1129 1132 Cuts at: 4555 5444 7073 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1132 1129 889 464 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4555 1537 Size: 275 2743 4090 Fragments arranged by size: 4090 2743 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2090 2665 3477 3513 3909 4044 4369 5038 Size: 737 575 812 36 396 135 325 669 Cuts at: 5038 1353 Size: 3423 Fragments arranged by size: 3423 812 737 669 575 396 325 135 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5695 6069 6215 387 Size: 1064 138 4106 374 146 1280 Fragments arranged by size: 4106 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3024 3471 3024 Size: 447 6661 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2403 2603 2793 2883 3420 3430 3849 Size: 393 771 200 190 90 537 10 419 Cuts at: 3849 4324 4365 5171 5768 6555 6823 1239 Size: 475 41 806 597 787 268 1524 Fragments arranged by size: 1524 806 787 771 597 537 475 419 393 268 200 190 90 41 10 EcoNI CCTnn'n_nnAGG Cuts at: 2043 2252 2528 3828 2043 Size: 209 276 1300 5323 Fragments arranged by size: 5323 1300 276 209 EcoO109I rG'GnC_Cy Cuts at: 3386 3728 3806 3386 Size: 342 78 6688 Fragments arranged by size: 6688 342 78 EcoRI G'AATT_C Cuts at: 4417 4795 4417 Size: 378 6730 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3056 3056 Size: 7108 FspI TGC'GCA Cuts at: 5614 5614 Size: 7108 HaeII r_GCGC'y Cuts at: 1678 2618 4108 4332 6255 1678 Size: 940 1490 224 1923 2531 Fragments arranged by size: 2531 1923 1490 940 224 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2254 2286 2769 2801 4340 Size: 32 135 32 682 32 483 32 1539 Cuts at: 4340 4372 4385 4417 5749 5781 5823 5855 1373 Size: 32 13 32 1332 32 42 32 2626 Fragments arranged by size: 2626 1539 1332 682 483 135 42 32 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 4452 4698 669 Size: 988 281 2514 246 3079 Fragments arranged by size: 3079 2514 988 281 246 HindIII A'AGCT_T Cuts at: 748 3038 748 Size: 2290 4818 HpaI GTT'AAC Cuts at: 4698 4698 Size: 7108 KpnI G_GTAC'C Cuts at: 4433 4433 Size: 7108 MfeI C'AATT_G Cuts at: 4707 4707 Size: 7108 MluI A'CGCG_T Cuts at: 6984 6984 Size: 7108 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3297 6222 6406 1305 Size: 1992 2925 184 2007 Fragments arranged by size: 2925 2007 1992 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2296 3425 10 Size: 54 1425 343 464 1129 3693 Fragments arranged by size: 3693 1425 1129 464 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3063 5066 5425 5584 518 Size: 648 1897 2003 359 159 2042 Fragments arranged by size: 2042 2003 1897 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2239 3952 4236 4524 5201 6349 6594 1606 Size: 633 1713 284 288 677 1148 245 2120 Fragments arranged by size: 2120 1713 1148 677 633 288 284 245 NcoI C'CATG_G Cuts at: 513 1065 3183 513 Size: 552 2118 4438 Fragments arranged by size: 4438 2118 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7108 NheI G'CTAG_C Cuts at: 1052 2903 1052 Size: 1851 5257 NotI GC'GGCC_GC Cuts at: 4555 4555 Size: 7108 NspI r_CATG'y Cuts at: 1132 1229 3101 4466 6011 1132 Size: 97 1872 1365 1545 2229 Fragments arranged by size: 2229 1872 1545 1365 97 PasI CC'CwG_GG Cuts at: 1348 2039 3619 1348 Size: 691 1580 4837 Fragments arranged by size: 4837 1580 691 PciI A'CATG_T Cuts at: 6007 6007 Size: 7108 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7108 PmeI GTTT'AAAC Cuts at: 4412 4412 Size: 7108 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5177 5209 6690 6722 5177 Size: 32 1481 32 5563 Fragments arranged by size: 5563 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7108 PsiI TTA'TAA Cuts at: 2354 4678 2354 Size: 2324 4784 PspOMI G'GGCC_C Cuts at: 2071 2071 Size: 7108 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3209 3241 3209 Size: 32 7076 PstI C_TGCA'G Cuts at: 830 2803 4460 830 Size: 1973 1657 3478 Fragments arranged by size: 3478 1973 1657 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7108 PvuII CAG'CTG Cuts at: 2239 3952 2239 Size: 1713 5395 SacI G_AGCT'C Cuts at: 3026 3473 3026 Size: 447 6661 SalI G'TCGA_C Cuts at: 1936 4450 1936 Size: 2514 4594 SbfI CC_TGCA'GG Cuts at: 4460 4460 Size: 7108 ScaI AGT'ACT Cuts at: 5356 7068 5356 Size: 1712 5396 SfcI C'TryA_G Cuts at: 826 1045 2525 2799 2874 3329 3572 3888 4456 Size: 219 1480 274 75 455 243 316 568 Cuts at: 4456 5591 6272 6463 826 Size: 1135 681 191 1471 Fragments arranged by size: 1480 1471 1135 681 568 455 316 274 243 219 191 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7108 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7108 SmaI CCC'GGG Cuts at: 1872 4435 1872 Size: 2563 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2756 2921 4076 4100 5212 6113 Size: 197 926 813 165 1155 24 1112 901 Cuts at: 6113 6375 820 Size: 262 1553 Fragments arranged by size: 1553 1155 1112 926 901 813 262 197 165 24 SnaBI TAC'GTA Cuts at: 493 4127 4899 493 Size: 3634 772 2702 Fragments arranged by size: 3634 2702 772 SpeI A'CTAG_T Cuts at: 152 152 Size: 7108 SphI G_CATG'C Cuts at: 1132 3101 4466 1132 Size: 1969 1365 3774 Fragments arranged by size: 3774 1969 1365 SspI AAT'ATT Cuts at: 5 52 5032 5 Size: 47 4980 2081 Fragments arranged by size: 4980 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7108 StyI C'CwwG_G Cuts at: 513 1065 3183 3731 4547 513 Size: 552 2118 548 816 3074 Fragments arranged by size: 3074 2118 816 552 548 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2745 5135 5152 5305 5490 5935 325 Size: 1255 1165 2390 17 153 185 445 1498 Fragments arranged by size: 2390 1498 1255 1165 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3285 3592 5354 Size: 274 80 33 51 1443 1308 307 1762 Cuts at: 5354 7066 96 Size: 1712 138 Fragments arranged by size: 1762 1712 1443 1308 307 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7068 7100 7068 Size: 32 7076 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7108 XbaI T'CTAG_A Cuts at: 4444 4444 Size: 7108 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7108 XmaI C'CCGG_G Cuts at: 1870 4433 1870 Size: 2563 4545 XmnI GAAnn'nnTTC Cuts at: 3040 3582 5237 3040 Size: 542 1655 4911 Fragments arranged by size: 4911 1655 542 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4916 276 Size: 53 83 186 936 3382 2468 Fragments arranged by size: 3382 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BfrBI BsiWI BstXI DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI