(Circular) (Six-base) MAPSORT of: pFN21AB3860.seq Check: 3201 from: 1 to: 5749 pFN21AB3860 5749 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2549 2733 2549 Size: 184 5565 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3559 278 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2154 3092 1937 Size: 217 938 4594 Fragments arranged by size: 4594 938 217 Acc65I G'GTAC_C Cuts at: 2121 3070 2121 Size: 949 4800 AclI AA'CG_TT Cuts at: 1563 3876 4249 1563 Size: 2313 373 3063 Fragments arranged by size: 3063 2313 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3812 5196 5464 1632 Size: 2180 1384 268 1917 Fragments arranged by size: 2180 1917 1384 268 AfeI AGC'GCT Cuts at: 2927 2927 Size: 5749 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5552 AflIII A'CryG_T Cuts at: 1203 2043 2577 2697 4648 5625 1203 Size: 840 534 120 1951 977 1327 Fragments arranged by size: 1951 1327 977 840 534 120 AgeI A'CCGG_T Cuts at: 4580 4580 Size: 5749 AhdI GACnn_n'nnGTC Cuts at: 4478 4478 Size: 5749 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5749 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5749 AlwNI CAG_nnn'CTG Cuts at: 2219 2255 2564 3492 5064 2219 Size: 36 309 928 1572 2904 Fragments arranged by size: 2904 1572 928 309 36 ApaI G_GGCC'C Cuts at: 2145 2145 Size: 5749 ApaLI G'TGCA_C Cuts at: 3806 4962 3806 Size: 1156 4593 ApoI r'AATT_y Cuts at: 1493 3058 3284 3436 5545 1493 Size: 1565 226 152 2109 1697 Fragments arranged by size: 2109 1697 1565 226 152 AscI GG'CGCG_CC Cuts at: 2100 2100 Size: 5749 AseI AT'TA_AT Cuts at: 160 1032 4303 160 Size: 872 3271 1606 Fragments arranged by size: 3271 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 AvaI C'yCGr_G Cuts at: 1870 1943 3074 1870 Size: 73 1131 4545 Fragments arranged by size: 4545 1131 73 BamHI G'GATC_C Cuts at: 2486 3079 3446 2486 Size: 593 367 4789 Fragments arranged by size: 4789 593 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2121 2528 2996 3070 4525 Size: 325 217 627 334 407 468 74 1455 Cuts at: 4525 618 Size: 1842 Fragments arranged by size: 1842 1455 627 468 407 334 325 217 74 BanII G_rGCy'C Cuts at: 1391 2145 2205 3068 1391 Size: 754 60 863 4072 Fragments arranged by size: 4072 863 754 60 BbeI G_GCGC'C Cuts at: 3000 3000 Size: 5749 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4778 BbvCI CC'TCA_GC Cuts at: 2126 2126 Size: 5749 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2077 2111 2839 2873 3940 Size: 34 50 34 56 34 728 34 1067 Cuts at: 3940 3974 1903 Size: 34 3678 Fragments arranged by size: 3678 1067 728 56 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3641 4851 3641 Size: 1210 4539 BclI T'GATC_A Cuts at: 1548 1548 Size: 5749 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2429 2508 4360 Size: 107 122 71 631 605 757 79 1852 Cuts at: 4360 136 Size: 1525 Fragments arranged by size: 1852 1525 757 631 605 122 107 79 71 BglII A'GATC_T Cuts at: 5744 5744 Size: 5749 BlpI GC'TnA_GC Cuts at: 3032 3166 3032 Size: 134 5615 Bme1580I G_kGCm'C Cuts at: 1790 2145 3810 4966 1790 Size: 355 1665 1156 2573 Fragments arranged by size: 2573 1665 1156 355 BmgBI CAC'GTC Cuts at: 1318 2610 2682 1318 Size: 1292 72 4385 Fragments arranged by size: 4385 1292 72 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3480 4438 442 Size: 416 53 83 399 24 2063 958 1753 Fragments arranged by size: 2063 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5749 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 4409 1239 Size: 845 2325 2579 Fragments arranged by size: 2579 2325 845 Bpu10I CC'TnA_GC Cuts at: 2126 2621 2126 Size: 495 5254 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2933 3874 4739 5037 2933 Size: 941 865 298 3645 Fragments arranged by size: 3645 941 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4412 882 Size: 617 2913 2219 Fragments arranged by size: 2913 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2137 2698 3540 493 Size: 711 933 561 842 2702 Fragments arranged by size: 2702 933 842 711 561 BsaBI GATnn'nnATC Cuts at: 5743 5743 Size: 5749 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2166 2349 2448 2997 Size: 53 83 186 936 633 183 99 549 Cuts at: 2997 3556 3938 4573 275 Size: 559 382 635 1451 Fragments arranged by size: 1451 936 635 633 559 549 382 186 183 99 83 53 BsaWI w'CCGG_w Cuts at: 4182 4580 4854 5001 5412 4182 Size: 398 274 147 411 4519 Fragments arranged by size: 4519 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2192 2222 1178 Size: 30 165 30 789 30 4705 Fragments arranged by size: 4705 789 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5749 BseYI C'CCAG_C Cuts at: 2233 4952 5448 2233 Size: 2719 496 2534 Fragments arranged by size: 2719 2534 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2093 3062 1163 Size: 573 357 969 3850 Fragments arranged by size: 3850 969 573 357 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2058 2325 2572 2866 3199 3960 Size: 275 186 57 267 247 294 333 761 Cuts at: 3960 4988 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 333 294 275 267 247 186 57 BsiHKAI G_wGCw'C Cuts at: 3810 3895 4966 3810 Size: 85 1071 4593 Fragments arranged by size: 4593 1071 85 BsmI GAATG_Cn' Cuts at: 2896 3260 3353 2896 Size: 364 93 5292 Fragments arranged by size: 5292 364 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2205 3068 3810 3895 4966 1391 Size: 399 355 60 863 742 85 1071 2174 Fragments arranged by size: 2174 1071 863 742 399 355 85 60 BspEI T'CCGG_A Cuts at: 5412 5412 Size: 5749 BspHI T'CATG_A Cuts at: 3638 5368 3638 Size: 1730 4019 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2549 2733 2748 2883 3104 844 Size: 1705 184 15 135 221 3489 Fragments arranged by size: 3489 1705 221 184 135 15 BsrBI CCG'CTC Cuts at: 1394 1823 2411 2868 3636 1394 Size: 429 588 457 768 3507 Fragments arranged by size: 3507 768 588 457 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4244 4418 65 Size: 1183 2996 174 1396 Fragments arranged by size: 2996 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2396 2571 2707 2816 4393 4580 1700 Size: 110 586 175 136 109 1577 187 2869 Fragments arranged by size: 2869 1577 586 187 175 136 110 109 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5749 BssHII G'CGCG_C Cuts at: 1925 2100 2515 2537 2539 2838 2878 2880 1925 Size: 175 415 22 2 299 40 2 4794 Fragments arranged by size: 4794 415 299 175 40 22 2 2 BssSI C'ACGA_G Cuts at: 3809 4821 3809 Size: 1012 4737 BstAPI GCAn_nnn'nTGC Cuts at: 794 2255 2740 2928 794 Size: 1461 485 188 3615 Fragments arranged by size: 3615 1461 485 188 BstBI TT'CG_AA Cuts at: 4553 4553 Size: 5749 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5749 BstYI r'GATC_y Cuts at: 1972 2486 3079 3446 3831 3848 5300 5386 5398 Size: 514 593 367 385 17 1452 86 12 Cuts at: 5398 5744 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 593 514 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2712 3009 513 Size: 552 1647 297 3253 Fragments arranged by size: 3253 1647 552 297 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5010 Fragments arranged by size: 5010 683 56 BtsI GCAGTG_nn' Cuts at: 2105 2842 3265 4059 4079 2105 Size: 737 423 794 20 3775 Fragments arranged by size: 3775 794 737 423 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5749 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5714 DraI TTT'AAA Cuts at: 3053 3053 Size: 5749 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2324 4756 809 Size: 507 1008 2432 1802 Fragments arranged by size: 2432 1802 1008 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2286 2322 Size: 54 1425 50 275 18 225 231 36 Cuts at: 2322 2569 2709 2715 3196 4085 5714 8 Size: 247 140 6 481 889 1629 43 Fragments arranged by size: 1629 1425 889 481 275 247 231 225 140 54 50 43 36 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2055 2322 2569 3196 1537 Size: 275 243 267 247 627 4090 Fragments arranged by size: 4090 627 275 267 247 243 EarI CTCTTCn'nnn_ Cuts at: 1353 2056 2930 3679 1353 Size: 703 874 749 3423 Fragments arranged by size: 3423 874 749 703 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2360 4336 4710 4856 387 Size: 1064 138 771 1976 374 146 1280 Fragments arranged by size: 1976 1280 1064 771 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 3812 4409 5196 5464 1239 Size: 393 452 1728 597 787 268 1524 Fragments arranged by size: 1728 1524 787 597 452 393 268 EcoO109I rG'GnC_Cy Cuts at: 2013 2141 2142 3005 2013 Size: 128 1 863 4757 Fragments arranged by size: 4757 863 128 1 EcoRI G'AATT_C Cuts at: 3058 3436 3058 Size: 378 5371 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2956 2956 Size: 5749 FspI TGC'GCA Cuts at: 4255 4255 Size: 5749 HaeII r_GCGC'y Cuts at: 1678 2073 2374 2674 2929 3000 4896 1678 Size: 395 301 300 255 71 1896 2531 Fragments arranged by size: 2531 1896 395 301 300 255 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2033 2065 2357 2389 4390 Size: 32 135 32 461 32 292 32 2001 Cuts at: 4390 4422 4464 4496 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2001 461 292 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3093 3339 669 Size: 988 281 1155 246 3079 Fragments arranged by size: 3079 1155 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5749 HpaI GTT'AAC Cuts at: 3339 3339 Size: 5749 KasI G'GCGC_C Cuts at: 2996 2996 Size: 5749 KpnI G_GTAC'C Cuts at: 2125 3074 2125 Size: 949 4800 MfeI C'AATT_G Cuts at: 3348 3348 Size: 5749 MluI A'CGCG_T Cuts at: 2043 2577 5625 2043 Size: 534 3048 2167 Fragments arranged by size: 3048 2167 534 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2895 4863 5047 1305 Size: 1590 1968 184 2007 Fragments arranged by size: 2007 1968 1590 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2288 2717 10 Size: 54 1425 343 456 429 3042 Fragments arranged by size: 3042 1425 456 429 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2717 3707 4066 4225 518 Size: 648 1551 990 359 159 2042 Fragments arranged by size: 2042 1551 990 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2219 2294 2662 3011 3165 3842 4990 5235 Size: 613 75 368 349 154 677 1148 245 Cuts at: 5235 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 613 368 349 245 154 75 NaeI GCC'GGC Cuts at: 2398 2573 2709 2398 Size: 175 136 5438 Fragments arranged by size: 5438 175 136 NarI GG'CG_CC Cuts at: 2997 2997 Size: 5749 NcoI C'CATG_G Cuts at: 513 1065 2712 513 Size: 552 1647 3550 Fragments arranged by size: 3550 1647 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5749 NgoMIV G'CCGG_C Cuts at: 2396 2571 2707 2396 Size: 175 136 5438 Fragments arranged by size: 5438 175 136 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5749 NotI GC'GGCC_GC Cuts at: 3196 3196 Size: 5749 NspI r_CATG'y Cuts at: 1132 1229 2806 2890 3107 4652 1132 Size: 97 1577 84 217 1545 2229 Fragments arranged by size: 2229 1577 1545 217 97 84 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5749 PciI A'CATG_T Cuts at: 4648 4648 Size: 5749 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5749 PmeI GTTT'AAAC Cuts at: 3053 3053 Size: 5749 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3818 3850 5331 5363 3818 Size: 32 1481 32 4204 Fragments arranged by size: 4204 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2013 2013 Size: 5749 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5749 PsiI TTA'TAA Cuts at: 3319 3319 Size: 5749 PspOMI G'GGCC_C Cuts at: 2141 2141 Size: 5749 PstI C_TGCA'G Cuts at: 830 2251 2796 3101 830 Size: 1421 545 305 3478 Fragments arranged by size: 3478 1421 545 305 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5749 PvuII CAG'CTG Cuts at: 2219 2662 2219 Size: 443 5306 SacII CC_GC'GG Cuts at: 3012 3012 Size: 5749 SalI G'TCGA_C Cuts at: 1936 3091 1936 Size: 1155 4594 SapI GCTCTTCn'nnn_ Cuts at: 2930 2930 Size: 5749 SbfI CC_TGCA'GG Cuts at: 3101 3101 Size: 5749 ScaI AGT'ACT Cuts at: 3997 5709 3997 Size: 1712 4037 SfcI C'TryA_G Cuts at: 826 1045 2247 2792 3097 4232 4913 5104 826 Size: 219 1202 545 305 1135 681 191 1471 Fragments arranged by size: 1471 1202 1135 681 545 305 219 191 SfoI GGC'GCC Cuts at: 2998 2998 Size: 5749 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 SgrAI Cr'CCGG_yG Cuts at: 1700 2816 1700 Size: 1116 4633 SmaI CCC'GGG Cuts at: 1872 3076 1872 Size: 1204 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2948 3853 4754 5016 820 Size: 197 926 1005 905 901 262 1553 Fragments arranged by size: 1553 1005 926 905 901 262 197 SnaBI TAC'GTA Cuts at: 493 3540 493 Size: 3047 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5749 SphI G_CATG'C Cuts at: 1132 3107 1132 Size: 1975 3774 SspI AAT'ATT Cuts at: 5 52 3673 5 Size: 47 3621 2081 Fragments arranged by size: 3621 2081 47 StuI AGG'CCT Cuts at: 1517 2855 1517 Size: 1338 4411 StyI C'CwwG_G Cuts at: 513 1065 2712 3188 513 Size: 552 1647 476 3074 Fragments arranged by size: 3074 1647 552 476 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3776 3793 3946 4131 4576 325 Size: 1255 2196 17 153 185 445 1498 Fragments arranged by size: 2196 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3995 5707 96 Size: 274 80 33 51 1443 2018 1712 138 Fragments arranged by size: 2018 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5709 5741 5709 Size: 32 5717 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5749 XbaI T'CTAG_A Cuts at: 3085 3085 Size: 5749 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2017 2710 2017 Size: 693 5056 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5749 XmaI C'CCGG_G Cuts at: 1870 3074 1870 Size: 1204 4545 XmnI GAAnn'nnTTC Cuts at: 2828 2939 3878 2828 Size: 111 939 4699 Fragments arranged by size: 4699 939 111 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3557 276 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI