Restriction map for
pFN21AB3856
(Circular) (Six-base) MAPSORT of: pFN21AB3856.seq Check: 9277 from: 1 to: 8428
pFN21AB3856 8428 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2277 2277
Size: 8428
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6238 278
Size: 53 83 186 936 4702 2468
Fragments arranged by size:
4702 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5771 1937
Size: 3834 4594
Acc65I G'GTAC_C
Cuts at: 5749 5749
Size: 8428
AclI AA'CG_TT
Cuts at: 1563 6555 6928 1563
Size: 4992 373 3063
Fragments arranged by size:
4992 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2693 3831 4035 6491 7875 8143 1632
Size: 1061 1138 204 2456 1384 268 1917
Fragments arranged by size:
2456 1917 1384 1138 1061 268 204
AfeI AGC'GCT
Cuts at: 3080 4248 3080
Size: 1168 7260
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8231
AflIII A'CryG_T
Cuts at: 1203 7327 8304 1203
Size: 6124 977 1327
Fragments arranged by size:
6124 1327 977
AgeI A'CCGG_T
Cuts at: 2832 7259 2832
Size: 4427 4001
AhdI GACnn_n'nnGTC
Cuts at: 7157 7157
Size: 8428
AleI CACnn'nnGTG
Cuts at: 1166 3294 1166
Size: 2128 6300
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3851 5627 1184
Size: 2667 1776 3985
Fragments arranged by size:
3985 2667 1776
AlwNI CAG_nnn'CTG
Cuts at: 2755 2797 3184 3947 4129 4213 4288 4888 6171
Size: 42 387 763 182 84 75 600 1283
Cuts at: 6171 7743 2755
Size: 1572 3440
Fragments arranged by size:
3440 1572 1283 763 600 387 182 84
75 42
ApaI G_GGCC'C
Cuts at: 2078 2309 2416 2575 2941 2953 3689 4314 2078
Size: 231 107 159 366 12 736 625 6192
Fragments arranged by size:
6192 736 625 366 231 159 107 12
ApaLI G'TGCA_C
Cuts at: 6485 7641 6485
Size: 1156 7272
ApoI r'AATT_y
Cuts at: 1493 3047 4763 5364 5737 5963 6115 8224 1493
Size: 1554 1716 601 373 226 152 2109 1697
Fragments arranged by size:
2109 1716 1697 1554 601 373 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6982 160
Size: 872 5950 1606
Fragments arranged by size:
5950 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8428
AvaI C'yCGr_G
Cuts at: 1870 1943 2096 2332 2415 2556 2659 2763 2934
Size: 73 153 236 83 141 103 104 171
Cuts at: 2934 2946 3688 4686 5753 1870
Size: 12 742 998 1067 4545
Fragments arranged by size:
4545 1067 998 742 236 171 153 141
104 103 83 73 12
BamHI G'GATC_C
Cuts at: 4794 5400 5758 6125 4794
Size: 606 358 367 7097
Fragments arranged by size:
7097 606 367 358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2022 2328 2654 2664 2691
Size: 325 217 627 235 306 326 10 27
Cuts at: 2691 3649 3655 4074 4277 4733 4822 5230 5749
Size: 958 6 419 203 456 89 408 519
Cuts at: 5749 7204 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 958 627 519 456 419 408
326 325 306 235 217 203 89 27
10 6
BanII G_rGCy'C
Cuts at: 1391 2078 2309 2416 2422 2575 2941 2953 3018
Size: 687 231 107 6 153 366 12 65
Cuts at: 3018 3132 3271 3350 3689 3960 4314 4967 5093
Size: 114 139 79 339 271 354 653 126
Cuts at: 5093 5747 1391
Size: 654 4072
Fragments arranged by size:
4072 687 654 653 366 354 339 271
231 153 139 126 114 107 79 65
12 6
BbeI G_GCGC'C
Cuts at: 2026 2332 2668 4078 2026
Size: 306 336 1410 6376
Fragments arranged by size:
6376 1410 336 306
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2115 2217 4208 4255 5008 928
Size: 971 216 102 1991 47 753 4348
Fragments arranged by size:
4348 1991 971 753 216 102 47
BbvCI CC'TCA_GC
Cuts at: 2406 2406
Size: 8428
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2636 2670 6619 6653 1903
Size: 34 699 34 3949 34 3678
Fragments arranged by size:
3949 3678 699 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6320 7530 6320
Size: 1210 7218
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8428
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2326 2402 3022
Size: 107 122 71 631 605 654 76 620
Cuts at: 3022 3152 3776 5564 7039 136
Size: 130 624 1788 1475 1525
Fragments arranged by size:
1788 1525 1475 654 631 624 620 605
130 122 107 76 71
BglII A'GATC_T
Cuts at: 4479 4539 8423 4479
Size: 60 3884 4484
Fragments arranged by size:
4484 3884 60
BlpI GC'TnA_GC
Cuts at: 2745 4570 4889 5845 2745
Size: 1825 319 956 5328
Fragments arranged by size:
5328 1825 956 319
Bme1580I G_kGCm'C
Cuts at: 1790 2078 2309 2416 2575 2694 2941 2953 3689
Size: 288 231 107 159 119 247 12 736
Cuts at: 3689 4282 4314 4827 5146 6489 7645 1790
Size: 593 32 513 319 1343 1156 2573
Fragments arranged by size:
2573 1343 1156 736 593 513 319 288
247 231 159 119 107 32 12
BmgBI CAC'GTC
Cuts at: 1318 3061 3397 1318
Size: 1743 336 6349
Fragments arranged by size:
6349 1743 336
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2748 3013 4893
Size: 416 53 83 399 24 1331 265 1880
Cuts at: 4893 6159 7117 442
Size: 1266 958 1753
Fragments arranged by size:
1880 1753 1331 1266 958 416 399 265
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8428
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3601 3601
Size: 8428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2370 3146 3453 3458 3545 3974 4235 4385
Size: 1131 776 307 5 87 429 261 150
Cuts at: 4385 4454 7088 1239
Size: 69 2634 2579
Fragments arranged by size:
2634 2579 1131 776 429 307 261 150
87 69 5
Bpu10I CC'TnA_GC
Cuts at: 2406 2406
Size: 8428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3890 6553 7418 7716 3890
Size: 2663 865 298 4602
Fragments arranged by size:
4602 2663 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2175 3422 4511 7091 882
Size: 617 676 1247 1089 2580 2219
Fragments arranged by size:
2580 2219 1247 1089 676 617
BsaAI yAC'GTr
Cuts at: 493 1204 3097 6219 493
Size: 711 1893 3122 2702
Fragments arranged by size:
3122 2702 1893 711
BsaBI GATnn'nnATC
Cuts at: 3221 8422 3221
Size: 5201 3227
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2023 2084 2329 2665
Size: 53 83 186 936 490 61 245 336
Cuts at: 2665 4075 6235 6617 7252 275
Size: 1410 2160 382 635 1451
Fragments arranged by size:
2160 1451 1410 936 635 490 382 336
245 186 83 61 53
BsaWI w'CCGG_w
Cuts at: 2275 2493 2832 5478 6861 7259 7533 7680 8091
Size: 218 339 2646 1383 398 274 147 411
Cuts at: 8091 2275
Size: 2612
Fragments arranged by size:
2646 2612 1383 411 398 339 274 218
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2153 2183 3005 3035 3086
Size: 30 165 30 750 30 822 30 51
Cuts at: 3086 3116 1178
Size: 30 6490
Fragments arranged by size:
6490 822 750 165 51 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 2198 2384 2443 2544 2609 2731 3080
Size: 976 36 186 59 101 65 122 349
Cuts at: 3080 3083 3878 3932 4343 1186
Size: 3 795 54 411 5271
Fragments arranged by size:
5271 976 795 411 349 186 122 101
65 59 54 36 3
BseYI C'CCAG_C
Cuts at: 2682 3081 3355 3682 3947 4192 4273 4281 4636
Size: 399 274 327 265 245 81 8 355
Cuts at: 4636 4661 4935 7631 8127 2682
Size: 25 274 2696 496 2983
Fragments arranged by size:
2983 2696 496 399 355 327 274 274
265 245 81 25 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2480 2511 3875 1163
Size: 573 744 31 1364 5716
Fragments arranged by size:
5716 1364 744 573 31
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2808 3724 5635 5878 6639 7667
Size: 275 186 807 916 1911 243 761 1028
Cuts at: 7667 1540
Size: 2301
Fragments arranged by size:
2301 1911 1028 916 807 761 275 243
186
BsiHKAI G_wGCw'C
Cuts at: 3132 3162 4554 4725 5358 5616 6489 6574 7645
Size: 30 1392 171 633 258 873 85 1071
Cuts at: 7645 3132
Size: 3915
Fragments arranged by size:
3915 1392 1071 873 633 258 171 85
30
BsmI GAATG_Cn'
Cuts at: 2884 3547 5939 6032 2884
Size: 663 2392 93 5280
Fragments arranged by size:
5280 2392 663 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4465 4465
Size: 8428
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2309 2416 2422 2575 2694 2941
Size: 399 288 231 107 6 153 119 247
Cuts at: 2941 2953 3018 3132 3162 3271 3350 3689 3960
Size: 12 65 114 30 109 79 339 271
Cuts at: 3960 4282 4314 4554 4725 4827 4967 5093 5146
Size: 322 32 240 171 102 140 126 53
Cuts at: 5146 5358 5616 5747 6489 6574 7645 1391
Size: 212 258 131 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 339 322 288 271
258 247 240 231 212 171 153 140
131 126 119 114 109 107 102 85
79 65 53 32 30 12 6
BspEI T'CCGG_A
Cuts at: 2275 5478 8091 2275
Size: 3203 2613 2612
Fragments arranged by size:
3203 2613 2612
BspHI T'CATG_A
Cuts at: 2820 5214 6317 8047 2820
Size: 2394 1103 1730 3201
Fragments arranged by size:
3201 2394 1730 1103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2277 3804 5118 5783 844
Size: 1433 1527 1314 665 3489
Fragments arranged by size:
3489 1527 1433 1314 665
BsrBI CCG'CTC
Cuts at: 1394 1823 2140 3495 3726 3787 5184 5416 5599
Size: 429 317 1355 231 61 1397 232 183
Cuts at: 5599 5686 6315 1394
Size: 87 629 3507
Fragments arranged by size:
3507 1397 1355 629 429 317 232 231
183 87 61
BsrDI GCAATG_nn'
Cuts at: 65 1248 6923 7097 65
Size: 1183 5675 174 1396
Fragments arranged by size:
5675 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2832 3652 3927 4730 4776 5671 7072
Size: 110 1022 820 275 803 46 895 1401
Cuts at: 7072 7259 1700
Size: 187 2869
Fragments arranged by size:
2869 1401 1022 895 820 803 275 187
110 46
BsrGI T'GTAC_A
Cuts at: 96 5373 96
Size: 5277 3151
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8428
BssSI C'ACGA_G
Cuts at: 2172 3509 3713 3761 6488 7500 2172
Size: 1337 204 48 2727 1012 3100
Fragments arranged by size:
3100 2727 1337 1012 204 48
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2755 2869 3184 4558 5428 794
Size: 1961 114 315 1374 870 3794
Fragments arranged by size:
3794 1961 1374 870 315 114
BstBI TT'CG_AA
Cuts at: 7232 7232
Size: 8428
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8428
BstXI CCAn_nnnn'nTGG
Cuts at: 5725 5725
Size: 8428
BstYI r'GATC_y
Cuts at: 1972 2151 3166 4479 4539 4794 5326 5340 5400
Size: 179 1015 1313 60 255 532 14 60
Cuts at: 5400 5758 6125 6510 6527 7979 8065 8077 8423
Size: 358 367 385 17 1452 86 12 346
Cuts at: 8423 1972
Size: 1977
Fragments arranged by size:
1977 1452 1313 1015 532 385 367 358
346 255 179 86 60 60 17 14
12
BtgI C'CryG_G
Cuts at: 513 1065 2772 2802 3148 3367 3552 3597 5127
Size: 552 1707 30 346 219 185 45 1530
Cuts at: 5127 513
Size: 3814
Fragments arranged by size:
3814 1707 1530 552 346 219 185 45
30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4729 4813 487
Size: 683 56 3503 84 4102
Fragments arranged by size:
4102 3503 683 84 56
BtsI GCAGTG_nn'
Cuts at: 2531 2631 2753 3645 5944 6738 6758 2531
Size: 100 122 892 2299 794 20 4201
Fragments arranged by size:
4201 2299 892 794 122 100 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8428
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8393
DraI TTT'AAA
Cuts at: 5732 5732
Size: 8428
DraIII CAC_nnn'GTG
Cuts at: 2869 2900 3205 2869
Size: 31 305 8092
Fragments arranged by size:
8092 305 31
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3395 7435 809
Size: 507 2079 4040 1802
Fragments arranged by size:
4040 2079 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2622 2799 2805
Size: 54 1425 50 275 18 792 177 6
Cuts at: 2805 2902 3721 3804 4131 4728 4789 5124 5349
Size: 97 819 83 327 597 61 335 225
Cuts at: 5349 5875 6764 8393 8
Size: 526 889 1629 43
Fragments arranged by size:
1629 1425 889 819 792 597 526 335
327 275 225 177 97 83 61 54
50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2805 3721 5875 1537
Size: 275 993 916 2154 4090
Fragments arranged by size:
4090 2154 993 916 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2819 3048 3677 4955 6358 1353
Size: 1466 229 629 1278 1403 3423
Fragments arranged by size:
3423 1466 1403 1278 629 229
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2615 2621 4048 7015 7389 7535
Size: 1064 138 1026 6 1427 2967 374 146
Cuts at: 7535 387
Size: 1280
Fragments arranged by size:
2967 1427 1280 1064 1026 374 146 138
6
EcoICRI GAG'CTC
Cuts at: 3130 3130
Size: 8428
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2370 2693 3146 3453 3458 3545 3831
Size: 393 738 323 453 307 5 87 286
Cuts at: 3831 3974 4035 4235 4385 4454 6491 7088 7875
Size: 143 61 200 150 69 2037 597 787
Cuts at: 7875 8143 1239
Size: 268 1524
Fragments arranged by size:
2037 1524 787 738 597 453 393 323
307 286 268 200 150 143 87 69
61 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2159 2159
Size: 8428
EcoO109I rG'GnC_Cy
Cuts at: 2075 2305 2412 2571 2767 2950 3138 3793 4210
Size: 230 107 159 196 183 188 655 417
Cuts at: 4210 4310 4311 2075
Size: 100 1 6192
Fragments arranged by size:
6192 655 417 230 196 188 183 159
107 100 1
EcoRI G'AATT_C
Cuts at: 5737 6115 5737
Size: 378 8050
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2132 4984 2132
Size: 2852 5576
FspI TGC'GCA
Cuts at: 4760 6934 4760
Size: 2174 6254
HaeII r_GCGC'y
Cuts at: 1678 2016 2026 2332 2668 3082 3898 3983 4078
Size: 338 10 306 336 414 816 85 95
Cuts at: 4078 4250 7575 1678
Size: 172 3325 2531
Fragments arranged by size:
3325 2531 816 414 338 336 306 172
95 85 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2153 2185 2497 2529 3947
Size: 32 135 32 581 32 312 32 1418
Cuts at: 3947 3979 5632 5664 7069 7101 7143 7175 1373
Size: 32 1653 32 1405 32 42 32 2626
Fragments arranged by size:
2626 1653 1418 1405 581 312 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5772 6018 669
Size: 988 281 3834 246 3079
Fragments arranged by size:
3834 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8428
HpaI GTT'AAC
Cuts at: 6018 6018
Size: 8428
KasI G'GCGC_C
Cuts at: 2022 2328 2664 4074 2022
Size: 306 336 1410 6376
Fragments arranged by size:
6376 1410 336 306
KpnI G_GTAC'C
Cuts at: 5753 5753
Size: 8428
MfeI C'AATT_G
Cuts at: 6027 6027
Size: 8428
MluI A'CGCG_T
Cuts at: 8304 8304
Size: 8428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3612 3624 4206 4602 7542 7726 1305
Size: 2307 12 582 396 2940 184 2007
Fragments arranged by size:
2940 2307 2007 582 396 184 12
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2624 2801 5126 10
Size: 54 1425 343 792 177 2325 3312
Fragments arranged by size:
3312 2325 1425 792 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3294 3366 3740 4844 5030 5213 5454
Size: 648 2128 72 374 1104 186 183 241
Cuts at: 5454 5609 5723 6386 6745 6904 518
Size: 155 114 663 359 159 2042
Fragments arranged by size:
2128 2042 1104 663 648 374 359 241
186 183 159 155 114 72
MspA1I CmG'CkG
Cuts at: 1606 3335 3482 4013 5272 5515 5555 5844 6521
Size: 1729 147 531 1259 243 40 289 677
Cuts at: 6521 7669 7914 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1729 1259 1148 677 531 289 245
243 147 40
NaeI GCC'GGC
Cuts at: 3929 4732 3929
Size: 803 7625
NarI GG'CG_CC
Cuts at: 2023 2329 2665 4075 2023
Size: 306 336 1410 6376
Fragments arranged by size:
6376 1410 336 306
NcoI C'CATG_G
Cuts at: 513 1065 3148 3367 3597 5127 513
Size: 552 2083 219 230 1530 3814
Fragments arranged by size:
3814 2083 1530 552 230 219
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8428
NgoMIV G'CCGG_C
Cuts at: 3927 4730 3927
Size: 803 7625
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8428
NotI GC'GGCC_GC
Cuts at: 5875 5875
Size: 8428
NspI r_CATG'y
Cuts at: 1132 1229 5080 5786 7331 1132
Size: 97 3851 706 1545 2229
Fragments arranged by size:
3851 2229 1545 706 97
PasI CC'CwG_GG
Cuts at: 1348 2078 2128 2302 3343 4951 1348
Size: 730 50 174 1041 1608 4825
Fragments arranged by size:
4825 1608 1041 730 174 50
PciI A'CATG_T
Cuts at: 7327 7327
Size: 8428
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2797 3205 3208 3482 4258 1108
Size: 1689 408 3 274 776 5278
Fragments arranged by size:
5278 1689 776 408 274 3
PfoI T'CCnGG_A
Cuts at: 2997 3123 3615 3819 4545 2997
Size: 126 492 204 726 6880
Fragments arranged by size:
6880 726 492 204 126
PmeI GTTT'AAAC
Cuts at: 5732 5732
Size: 8428
PmlI CAC'GTG
Cuts at: 3097 3097
Size: 8428
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6497 6529 8010 8042 6497
Size: 32 1481 32 6883
Fragments arranged by size:
6883 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2767 3138 3793 4210 2767
Size: 371 655 417 6985
Fragments arranged by size:
6985 655 417 371
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8428
PsiI TTA'TAA
Cuts at: 5998 5998
Size: 8428
PspOMI G'GGCC_C
Cuts at: 2074 2305 2412 2571 2937 2949 3685 4310 2074
Size: 231 107 159 366 12 736 625 6192
Fragments arranged by size:
6192 736 625 366 231 159 107 12
PstI C_TGCA'G
Cuts at: 830 2638 3830 3990 5085 5514 5780 830
Size: 1808 1192 160 1095 429 266 3478
Fragments arranged by size:
3478 1808 1192 1095 429 266 160
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8428
PvuII CAG'CTG
Cuts at: 3335 3482 4013 5515 5555 3335
Size: 147 531 1502 40 6208
Fragments arranged by size:
6208 1502 531 147 40
SacI G_AGCT'C
Cuts at: 3132 3132
Size: 8428
SalI G'TCGA_C
Cuts at: 1936 5770 1936
Size: 3834 4594
SanDI GG'GwC_CC
Cuts at: 2767 2767
Size: 8428
SapI GCTCTTCn'nnn_
Cuts at: 4955 4955
Size: 8428
SbfI CC_TGCA'GG
Cuts at: 5780 5780
Size: 8428
ScaI AGT'ACT
Cuts at: 6676 8388 6676
Size: 1712 6716
SexAI A'CCwGG_T
Cuts at: 3201 4371 3201
Size: 1170 7258
SfcI C'TryA_G
Cuts at: 826 1045 2156 2634 3826 3939 3986 4348 5081
Size: 219 1111 478 1192 113 47 362 733
Cuts at: 5081 5510 5776 6911 7592 7783 826
Size: 429 266 1135 681 191 1471
Fragments arranged by size:
1471 1192 1135 1111 733 681 478 429
362 266 219 191 113 47
SfoI GGC'GCC
Cuts at: 2024 2330 2666 4076 2024
Size: 306 336 1410 6376
Fragments arranged by size:
6376 1410 336 306
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8428
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8428
SmaI CCC'GGG
Cuts at: 1872 2334 2417 2765 2948 4688 5755 1872
Size: 462 83 348 183 1740 1067 4545
Fragments arranged by size:
4545 1740 1067 462 348 183 83
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3905 6532 7433 7695 820
Size: 197 926 1962 2627 901 262 1553
Fragments arranged by size:
2627 1962 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6219 493
Size: 5726 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8428
SphI G_CATG'C
Cuts at: 1132 5080 5786 1132
Size: 3948 706 3774
Fragments arranged by size:
3948 3774 706
SrfI GCCC'GGGC
Cuts at: 2417 2417
Size: 8428
SspI AAT'ATT
Cuts at: 5 52 6352 5
Size: 47 6300 2081
Fragments arranged by size:
6300 2081 47
StuI AGG'CCT
Cuts at: 1517 4334 1517
Size: 2817 5611
StyI C'CwwG_G
Cuts at: 513 1065 2031 3148 3367 3597 5127 5867 513
Size: 552 966 1117 219 230 1530 740 3074
Fragments arranged by size:
3074 1530 1117 966 740 552 230 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2268 2279 3306 4265 4487 5466 5649
Size: 1255 688 11 1027 959 222 979 183
Cuts at: 5649 6455 6472 6625 6810 7255 325
Size: 806 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1027 979 959 806 688 445
222 185 183 153 17 11
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5373 5703 6674
Size: 274 80 33 51 1443 3396 330 971
Cuts at: 6674 8386 96
Size: 1712 138
Fragments arranged by size:
3396 1712 1443 971 330 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2216 2243 2248 2275 2574 2606 3005 3037 3086
Size: 27 5 27 299 32 399 32 49
Cuts at: 3086 3118 4896 4928 8388 8420 2216
Size: 32 1778 32 3460 32 2224
Fragments arranged by size:
3460 2224 1778 399 299 49 32 32
32 32 32 27 27 5
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8428
XbaI T'CTAG_A
Cuts at: 5764 5764
Size: 8428
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4840 4840
Size: 8428
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8428
XmaI C'CCGG_G
Cuts at: 1870 2332 2415 2763 2946 4686 5753 1870
Size: 462 83 348 183 1740 1067 4545
Fragments arranged by size:
4545 1740 1067 462 348 183 83
XmnI GAAnn'nnTTC
Cuts at: 3308 4541 6557 3308
Size: 1233 2016 5179
Fragments arranged by size:
5179 2016 1233
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6236 276
Size: 53 83 186 936 4702 2468
Fragments arranged by size:
4702 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I
EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI
RsrII SacII SfiI SwaI