Restriction map for
pFN21AB3831
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# NO CUT ENZYMES ( 89 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AgeI ApoI AscI AseI
AsnI Asp700I Asp718I AvrII BbrPI
BciVI BclI BfrI BglII BlnI
BsaAI BsaI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI DraI EarI
EcoRI EcoRV EcoT22I FbaI HincII
HindII HindIII HpaI KpnI Ksp632I
MfeI MluI MroI MseI MunI
NdeI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I XbaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 214 GACGTC GACGT^C
AflIII 779 ACGTGT A^C[GA][CT]GT
AhdI 214 GACGTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2038 GTGCAC G^TGCAC
Aor51HI 938 AGCGCT AGC^GCT
ApaLI 2038 GTGCAC G^TGCAC
AspEI 214 GACGTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 787 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1361 TGCGCA TGC^GCA
BamHI 704 GGATCC G^GATCC
BbeI 763 GGCGCC GGCGC^C
BbsI 1212 GAAGAC GAAGAC(2/6)
BbvCI 2011 GCTGAGG CCTCAGC(-5/-2)
BcgI 636 CGATGGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1212 GAAGAC GAAGAC(2/6)
BsaBI 2092 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1735 GAGACG CGTCTC(1/5)
BsrDI 272 CATTGC GCAATG(2/0)
BsrI 1222 ACTGG ACTGG(1/-1)
BstEII 792 GGTCACC G^GT[ACGT]ACC
BstPI 792 GGTCACC G^GT[ACGT]ACC
BstYI 704 GGATCC [GA]^GATC[CT]
CpoI 47 CGGTCCG CG^G[AT]CCG
DpnII 705 GATC ^GATC
Eam1105I 214 GACGTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 938 AGCGCT AGC^GCT
EcoO65I 792 GGTCACC G^GT[ACGT]ACC
EcoT14I 647 CCATGG C^C[AT][AT]GG
FseI 1503 GGCCGGCC GGCCGG^CC
FspI 1361 TGCGCA TGC^GCA
HinfI 7 GAGTC G^A[ACGT]TC
KasI 763 GGCGCC G^GCGCC
MamI 2092 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 705 GATC ^GATC
MflI 704 GGATCC [GA]^GATC[CT]
NarI 763 GGCGCC GG^CGCC
NcoI 647 CCATGG C^CATGG
NdeII 705 GATC ^GATC
NspI 357 GCATGT [GA]CATG^[CT]
PaeR7I 948 CTCGAG C^TCGAG
PflFI 787 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 7 GAGTC GAGTC(4/5)
PshAI 1737 GACGGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 47 CGGTCCG CG^G[AT]CCG
Sau3AI 705 GATC ^GATC
SexAI 572 ACCAGGT A^CC[AT]GGT
SfoI 763 GGCGCC GGC^GCC
SmlI 948 CTCGAG C^T[CT][GA]AG
StyI 647 CCATGG C^C[AT][AT]GG
Tth111I 787 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 948 CTCGAG C^TCGAG
XhoII 704 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwI 705 GATCC GGATC(4/5)
704 GGATC
AocI 861 CCTGAGG CC^T[ACGT]AGG
988 CCTCAGG
BalI 902 TGGCCA TGG^CCA
1196 TGGCCA
BlpI 747 GCTGAGC GC^T[ACGT]AGC
1494 GCTCAGC
BpmI 421 CTCCAG CTGGAG(16/14)
2080 CTGGAG
Bpu1102I 747 GCTGAGC GC^T[ACGT]AGC
1494 GCTCAGC
BsiEI 1506 CGGCCG CG[GA][CT]^CG
1817 CGGCCG
BstXI 1067 CCATCTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1199 CCAAGAGCCTGG
Bsu36I 861 CCTGAGG CC^T[ACGT]AGG
988 CCTCAGG
CelII 747 GCTGAGC GC^T[ACGT]AGC
1494 GCTCAGC
DraIII 801 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1828 CACACAGTG
DrdI 1233 GACACAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1408 GACGCGCTGGTC
EagI 1506 CGGCCG C^GGCCG
1817 CGGCCG
EclXI 1506 CGGCCG C^GGCCG
1817 CGGCCG
Eco52I 1506 CGGCCG C^GGCCG
1817 CGGCCG
Eco81I 861 CCTGAGG CC^T[ACGT]AGG
988 CCTCAGG
EcoNI 1359 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1986 CCTCGGGGAGG
MluNI 902 TGGCCA TGG^CCA
1196 TGGCCA
MscI 902 TGGCCA TGG^CCA
1196 TGGCCA
NheI 822 GCTAGC G^CTAGC
1935 GCTAGC
SacI 418 GAGCTC GAGCT^C
835 GAGCTC
SmaI 71 CCCGGG CCC^GGG
1999 CCCGGG
XmaCI 71 CCCGGG C^CCGGG
1999 CCCGGG
XmaI 71 CCCGGG C^CCGGG
1999 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
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AccII AciI AcyI AfaI AluI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BglI BmyI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BssKI BssSI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI KspI
MaeI MaeII MaeIII MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NgoMIV NlaIII
NlaIV PflMI PpuMI PstI PvuII
RsaI SacII Sau96I ScrFI SfaNI
SfcI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XcmI